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chemidoc mp imaging system  (Bio-Rad)


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    Structured Review

    Bio-Rad chemidoc mp imaging system
    Chemidoc Mp Imaging System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 22784 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagers/ChemiDoc+MP+Imaging+System/pmc13004575-86-15-19
    Average 99 stars, based on 22784 article reviews
    chemidoc mp imaging system - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: A common germline 3' UTR variant controls DDIT4 translation efficiency and stress responses in cancer cells.
    Article Snippet: .. Immunoblotted membranes were developed with ECLTM Select western blot detection reagents (Amersham Biosciences, UK) and imaged on the ChemiDocTM XRS imaging system (Bio-Rad). ..

    Article Title: Elucidating the Molecular Impact of DA29‐Induced ClpP Chemoactivation on Mitochondrial Function
    Article Snippet: Whole‐cell proteins (20‐30 μg), mitochondrial lysates (30 μg) or sucrose gradient fractions (10 μL) were denatured in 4X Laemmli sample buffer (Bio‐Rad) complemented with 10% 2‐mercaptoethanol, separated by SDS‐PAGE and subjected to immunoblotting as described in [ ], using the following primary antibodies: ClpP (#A306224, Antibodies.com), ClpX (#ab168338, Abcam), total OXPHOS cocktail (#ab110413, Abcam), HA‐tag (#3724, Cell Signaling Technology), uL13m (#16241–1‐AP, Proteintech), VDAC1 (#ab186321, Abcam), mS29 (# MA141279 , Thermo Fisher Scientific), α‐tubulin (#T6074, Sigma‐Aldrich) and β‐actin (#A2066, Sigma‐Aldrich). .. Chemiluminescent signals were detected using the Clarity Western ECL substrate (Bio‐Rad) and the ChemiDoc MP Imaging System (Bio‐Rad). .. Densitometric analysis of band intensities was carried out by ImageJ 1.54p (RRID:SCR_003070) and GelQuant.NET 1.7.8 software.

    Imaging:

    Article Title: A common germline 3' UTR variant controls DDIT4 translation efficiency and stress responses in cancer cells.
    Article Snippet: .. Immunoblotted membranes were developed with ECLTM Select western blot detection reagents (Amersham Biosciences, UK) and imaged on the ChemiDocTM XRS imaging system (Bio-Rad). ..

    Article Title: Dapagliflozin alleviates CYH33-induced hyperglycemia and preserves antitumor immunity against ovarian cancer in type 1 diabetic mice.
    Article Snippet: PI3K is frequently hyperactivated in ovarian cancer and has emerged as a promising therapeutic target.. CYH33 is a novel PI3Kα-selective inhibitor that has been approved in Japan for the treatment of ovarian clear cell carcinoma harboring PIK3CA mutations.. However, its on-target adverse effect of hyperglycemia limits its clinical application, particularly in patients susceptible to diabetes, underscoring the urgent need to optimize therapeutic strategies to improve both safety and efficacy.

    Article Title: Alternative splicing of a species- and skeletal muscle-specific microexon diversifies the menu of clathrin heavy chains in mice and rats.
    Article Snippet: .. PCR products were resolved via 6% polyacrylamide gel electrophoresis using TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA pH 8.5) for 3–4 h at 150–160 V. Polyacrylamide gels were stained with 0.4 μg/mL ethidium bromide for 10 min. All gels were imaged under ultraviolet illumination in a ChemiDoc XRS+ Imaging System (Bio-Rad). .. Alternative splicing was quantified by densitometry using the Image Lab software (version 6.0.1, Bio-Rad).

    Article Title: Elucidating the Molecular Impact of DA29‐Induced ClpP Chemoactivation on Mitochondrial Function
    Article Snippet: Whole‐cell proteins (20‐30 μg), mitochondrial lysates (30 μg) or sucrose gradient fractions (10 μL) were denatured in 4X Laemmli sample buffer (Bio‐Rad) complemented with 10% 2‐mercaptoethanol, separated by SDS‐PAGE and subjected to immunoblotting as described in [ ], using the following primary antibodies: ClpP (#A306224, Antibodies.com), ClpX (#ab168338, Abcam), total OXPHOS cocktail (#ab110413, Abcam), HA‐tag (#3724, Cell Signaling Technology), uL13m (#16241–1‐AP, Proteintech), VDAC1 (#ab186321, Abcam), mS29 (# MA141279 , Thermo Fisher Scientific), α‐tubulin (#T6074, Sigma‐Aldrich) and β‐actin (#A2066, Sigma‐Aldrich). .. Chemiluminescent signals were detected using the Clarity Western ECL substrate (Bio‐Rad) and the ChemiDoc MP Imaging System (Bio‐Rad). .. Densitometric analysis of band intensities was carried out by ImageJ 1.54p (RRID:SCR_003070) and GelQuant.NET 1.7.8 software.

    Article Title: Aerobic exercise ameliorates cardiac function in HFpEF mice by suppressing ALCAT1, neuroinflammation and oxidative stress in the paraventricular nucleus and nucleus tractus solitarius.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Effect of Microcurrent Stimulation on Cartilage Regeneration in Engineered Chondrocyte Spheroids.
    Article Snippet: Protein bands were visualized using an enhanced chemiluminescence detection kit (Thermo Fisher Scientific, Waltham, MA, USA). .. and captured using a chemiluminescence imaging system (ChemiDoc MP, Bio-Rad, Hercules, CA, USA). .. Band intensities were 2026 quantified using ImageJ software and normalized to β-actin

    Article Title: Modified tumor uptake and biodistribution of nanoparticles coated with small extracellular vesicle membranes derived from distinct tumor cell lines
    Article Snippet: Membranes were then washed with tris buffered saline containing 0.1% tween-20 followed by 2 h of hybridization with 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rat IgG (NB7115, Novus Biological) or goat anti-mouse IgG (170–6516, BioRad). .. After washing, protein bands were visualized using SuperSignal West Femto (34096, ThermoFisher) and imaged on ChemiDoc MP imaging system (Bio-Rad). .. The signal intensity of bands were quantified utilizing ImageLab software (Bio-Rad).

    Article Title: OGT regulates RNA-binding protein nucleocytoplasmic partitioning, but this fails in amyotrophic lateral sclerosis.
    Article Snippet: Membranes were subsequently iScience 29, 117807, November 20, 2026 13 incubated overnight at 4◦C with the primary antibodies against OGT (1:500; Santa Cruz, SC-74546) or O-GlcNAc RL2 (1:1,000; Abcam, ab2739) diluted in 5% milk-TBS-T. Membranes were washed three times in TBS-T for 5 min each, followed by incubation for 1 h at room temperature with the corresponding HRP-conjugated secondary antibodies (1:5,000; Cytiva) in TBS-T. After three additional 5-min washes in TBS-T, signals were detected using ECL reagent (Cytiva, RPN2232). .. All membranes were imaged using a ChemiDoc MP imaging system (Bio-Rad). .. Band intensities were analyzed using Image Lab software (Bio-Rad).

    Polymerase Chain Reaction:

    Article Title: Alternative splicing of a species- and skeletal muscle-specific microexon diversifies the menu of clathrin heavy chains in mice and rats.
    Article Snippet: .. PCR products were resolved via 6% polyacrylamide gel electrophoresis using TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA pH 8.5) for 3–4 h at 150–160 V. Polyacrylamide gels were stained with 0.4 μg/mL ethidium bromide for 10 min. All gels were imaged under ultraviolet illumination in a ChemiDoc XRS+ Imaging System (Bio-Rad). .. Alternative splicing was quantified by densitometry using the Image Lab software (version 6.0.1, Bio-Rad).

    Polyacrylamide Gel Electrophoresis:

    Article Title: Alternative splicing of a species- and skeletal muscle-specific microexon diversifies the menu of clathrin heavy chains in mice and rats.
    Article Snippet: .. PCR products were resolved via 6% polyacrylamide gel electrophoresis using TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA pH 8.5) for 3–4 h at 150–160 V. Polyacrylamide gels were stained with 0.4 μg/mL ethidium bromide for 10 min. All gels were imaged under ultraviolet illumination in a ChemiDoc XRS+ Imaging System (Bio-Rad). .. Alternative splicing was quantified by densitometry using the Image Lab software (version 6.0.1, Bio-Rad).

    Staining:

    Article Title: Alternative splicing of a species- and skeletal muscle-specific microexon diversifies the menu of clathrin heavy chains in mice and rats.
    Article Snippet: .. PCR products were resolved via 6% polyacrylamide gel electrophoresis using TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA pH 8.5) for 3–4 h at 150–160 V. Polyacrylamide gels were stained with 0.4 μg/mL ethidium bromide for 10 min. All gels were imaged under ultraviolet illumination in a ChemiDoc XRS+ Imaging System (Bio-Rad). .. Alternative splicing was quantified by densitometry using the Image Lab software (version 6.0.1, Bio-Rad).

    Incubation:

    Article Title: Aerobic exercise ameliorates cardiac function in HFpEF mice by suppressing ALCAT1, neuroinflammation and oxidative stress in the paraventricular nucleus and nucleus tractus solitarius.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.



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