Journal: BMC Biotechnology

Article Title: Accurate non-invasive image-based cytotoxicity assays for cultured cells

doi: 10.1186/1472-6750-10-43

Figure Lengend Snippet: Bland-Altman plot (pEC 50 value difference CloneSelect Imager-MTT vs average) showing the comparison of the pEC 50 values produced by cisplatin in: (A) the A2780 cells, (B) the A2780R cells, after 48 h of incubation, tested with CloneSelect Imager system and MTT assay . The limits of agreement (bias ± 1.96 SD) are denoted with horizontal solid lines.

Article Snippet: In summary, the CloneSelect™ Imager system has been compared with the MTT assay, and it displays lower intra experimental deviations in the dose response curves, with high reproducibility, and showing that the CloneSelect Imager system is a much preferred alternative to determine the antiproliferative activity of different drugs and possibly more accurate.

Techniques: Produced, Incubation, MTT Assay

Journal: BMC Biotechnology

Article Title: Accurate non-invasive image-based cytotoxicity assays for cultured cells

doi: 10.1186/1472-6750-10-43

Figure Lengend Snippet: Comparison of the pEC 50 values obtained with the CloneSelect Imager system analysis and the MTT assay.

Article Snippet: In summary, the CloneSelect™ Imager system has been compared with the MTT assay, and it displays lower intra experimental deviations in the dose response curves, with high reproducibility, and showing that the CloneSelect Imager system is a much preferred alternative to determine the antiproliferative activity of different drugs and possibly more accurate.

Techniques: MTT Assay

Journal: BMC Biotechnology

Article Title: Accurate non-invasive image-based cytotoxicity assays for cultured cells

doi: 10.1186/1472-6750-10-43

Figure Lengend Snippet: Comparison of the dose response plot of cisplatin after 72 h in the A2780R cells . Both plots display cell viability relative to non-treated cells vs -log [cisplatin]. (A) measure with the MTT assay and (B) measure with the CloneSelect Imager system. The pEC 50 value is denoted at the crossing of the solid lines. The concentration of cisplatin used (8.47 nM, 25.4 nM, 76.2 nM, 0.229 μM, 0.686 μM, 2.06 μM, 6.17 μM, 18.5 μM, 55.6 μM, and 167 μM) plotted as -log C vs cell viability. The error bars indicate the accuracy and reproducibility.

Article Snippet: In summary, the CloneSelect™ Imager system has been compared with the MTT assay, and it displays lower intra experimental deviations in the dose response curves, with high reproducibility, and showing that the CloneSelect Imager system is a much preferred alternative to determine the antiproliferative activity of different drugs and possibly more accurate.

Techniques: MTT Assay, Concentration Assay

Journal: BMC Biotechnology

Article Title: Accurate non-invasive image-based cytotoxicity assays for cultured cells

doi: 10.1186/1472-6750-10-43

Figure Lengend Snippet: EC 50 (μM) values obtained after 48 h and 72 h incubation with cisplatin and oxaliplatin on the suspension cultures L1210/0 and the L1210/2 cells obtained, using the CloneSelect Imager system

Article Snippet: In summary, the CloneSelect™ Imager system has been compared with the MTT assay, and it displays lower intra experimental deviations in the dose response curves, with high reproducibility, and showing that the CloneSelect Imager system is a much preferred alternative to determine the antiproliferative activity of different drugs and possibly more accurate.

Techniques: Incubation