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imagequant las 4000 software  (Danaher Inc)


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    Danaher Inc imagequant las 4000 software
    Imagequant Las 4000 Software, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 97/100, based on 6553 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagequant+data+analysis+software/ImageQuant+LAS+4000/pmc04644493-80-4-8
    Average 97 stars, based on 6553 article reviews
    imagequant las 4000 software - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: Tyrosine Kinase Inhibitors Diminish Renal Neoplasms in a Tuberous Sclerosis Model Via Induction of Apoptosis
    Article Snippet: .. All membranes were washed with TBS-T, incubated with peroxidase (POD)-labeled secondary antibodies at room temperature, and visualized with Pierce ECL reagent (No. 32106, Thermo Fisher Scientific) and ImageQuant LAS 4000 Series imager (GE Healthcare Life Sciences). ..

    Article Title: Adipocytes promote pancreatic cancer migration and invasion through fatty acid metabolic reprogramming
    Article Snippet: A total of 20 μg protein was loaded per lane onto 12% SDS-gels, resolved using SDS-PAGE, transferred to 0.22 μm PVDF membranes (MilliporeSigma), and blocked for 1 h at room temperature with 5% skim milk. .. Each membrane was immunoblotted with the indicated primary antibodies at 4°C overnight and then incubated with a secondary antibody at 37°C for 1 h. Immunoreactive bands were visualized using an enhanced chemiluminescence detection kit (Thermo Fisher Scientific, Inc.) using an ImageQuant LAS 4000 System (GE Healthcare). .. The following primary and secondary antibodies were used in the present study: AKT (1:1,000; ProteinTech Group, Inc.; cat. no. 10176-2-AP), p-AKT Ser473 (1:1,000, Cell Signaling Technology, Inc.; cat. no. CST4060S), HIF-1α (1:1,000; ProteinTech Group, Inc.; cat. no. 20960-1-AP), ANGPTL4 (1:1,000; ProteinTech Group, Inc.; cat. no. 18374-1-AP), GLUT1 (1:1,000; ProteinTech Group, Inc.; cat. no. 66290-1-lg), HK2 (1:1,000; ProteinTech Group, Inc.; cat. no. 22029-1-AP), LDHA (1:1,000; ProteinTech Group, Inc.; cat. no. 19987-1-AP), α-tubulin (1:1,000; ProteinTech Group, Inc.; cat. no. 66031-1-lg), p-AMPKα Thr172 (1:1,000; Cell Signaling Technology, Inc.; cat. no. CST2535), AMPKα (1:1,000; ProteinTech Group, Inc.; cat. no. 10929-2-AP), p-STAT3 Tyr705 (1:1,000; Cell Signaling Technology, Inc.; cat. no. CST9145), STAT3 (1:1,000; Cell Signaling Technology, Inc.; CST12640), Snail (1:1,000; CST; CST9782), horseradish peroxidase-conjugated anti-rabbit IgG antibody (1:5,000; ProteinTech Group, Inc.; cat. no. SA00001-2) and goat anti-mouse IgG secondary antibody HRP conjugated (1:5,000; Signalway Antibody LLC; cat. no. L3032).

    Western Blot:

    Article Title: A leaf-emanated signal orchestrates grain size and number in response to maternal resources.
    Article Snippet: .. Signals were developed and detected using ECL Prime Western Blotting Detection Reagent and ImageQuant LAS 4000 (both GE Healthcare Life Sciences). ..

    Article Title: Selective Inhibition of ATM-dependent Double-strand Break Repair and Checkpoint Control Synergistically Enhances the Efficacy of ATR Inhibitors
    Article Snippet: The primary antibodies used were: p-ATM S1981 (Abcam #ab81292), ATM (Santa Cruz Biotechnology #SC23921), p-KAP1 S824 (Abcam #ab133440), KAP1 (Abcam #ab22553), p-CHK2 T68 (Cell Signaling Technology #2197), CHK2 (Cell Signaling Technology #6334), p-CHK1 S317 (Cell Signaling Technology #12302), CHK1 (Cell Signaling Technology #2360), p-p53 S15 (Cell Signaling Technology #9284), p53 (Cell Signaling Technology #48818), MDM2 (Cell Signaling Technology #86934), p21 (Cell Signaling Technology #2947), and horseradish peroxidase–conjugated GAPDH (Cell Signaling Technology #3683). .. Western blots were analyzed using GE ImageQuant LAS 4000 with SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific #34080) and/or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific #34094). .. Cell-cycle analysis was performed using the FITC bromodeoxyuridine (BrdU) Flow Kit from BD Biosciences (#559619) according to the manufacturer's protocol.

    Membrane:

    Article Title: Adipocytes promote pancreatic cancer migration and invasion through fatty acid metabolic reprogramming
    Article Snippet: A total of 20 μg protein was loaded per lane onto 12% SDS-gels, resolved using SDS-PAGE, transferred to 0.22 μm PVDF membranes (MilliporeSigma), and blocked for 1 h at room temperature with 5% skim milk. .. Each membrane was immunoblotted with the indicated primary antibodies at 4°C overnight and then incubated with a secondary antibody at 37°C for 1 h. Immunoreactive bands were visualized using an enhanced chemiluminescence detection kit (Thermo Fisher Scientific, Inc.) using an ImageQuant LAS 4000 System (GE Healthcare). .. The following primary and secondary antibodies were used in the present study: AKT (1:1,000; ProteinTech Group, Inc.; cat. no. 10176-2-AP), p-AKT Ser473 (1:1,000, Cell Signaling Technology, Inc.; cat. no. CST4060S), HIF-1α (1:1,000; ProteinTech Group, Inc.; cat. no. 20960-1-AP), ANGPTL4 (1:1,000; ProteinTech Group, Inc.; cat. no. 18374-1-AP), GLUT1 (1:1,000; ProteinTech Group, Inc.; cat. no. 66290-1-lg), HK2 (1:1,000; ProteinTech Group, Inc.; cat. no. 22029-1-AP), LDHA (1:1,000; ProteinTech Group, Inc.; cat. no. 19987-1-AP), α-tubulin (1:1,000; ProteinTech Group, Inc.; cat. no. 66031-1-lg), p-AMPKα Thr172 (1:1,000; Cell Signaling Technology, Inc.; cat. no. CST2535), AMPKα (1:1,000; ProteinTech Group, Inc.; cat. no. 10929-2-AP), p-STAT3 Tyr705 (1:1,000; Cell Signaling Technology, Inc.; cat. no. CST9145), STAT3 (1:1,000; Cell Signaling Technology, Inc.; CST12640), Snail (1:1,000; CST; CST9782), horseradish peroxidase-conjugated anti-rabbit IgG antibody (1:5,000; ProteinTech Group, Inc.; cat. no. SA00001-2) and goat anti-mouse IgG secondary antibody HRP conjugated (1:5,000; Signalway Antibody LLC; cat. no. L3032).



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    Effect of CADO and PGE2 on cAMP production (a) and CREB (b) and Csk (c) phosphorylation. a TILs #2009 at stage II were incubated with CADO (50 μM) and/or PGE2 (0.5 μM) or CGS (50 μM) and/or butaprost (5 μM) for 10 min. The levels of cAMP in cellular extracts were analyzed using mass spectrometry. b, c TILs #2009 at stage II were stimulated with anti-CD3 in the presence of CADO (50 μM) and/or PGE2 (0.5 μM) for 30 min. TIL protein extracts (50 μg) were resolved using 10% SDS-PAGE and transferred to PVDF membranes. The level of CREB (b) and Csk (c) phosphorylation was analyzed using specific antibodies. The optic density (OD) ratio of the bands was analyzed using <t>ImageQuanT</t> data analysis software (Molecular Dynamics)
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    Image Search Results


    Effect of CADO and PGE2 on cAMP production (a) and CREB (b) and Csk (c) phosphorylation. a TILs #2009 at stage II were incubated with CADO (50 μM) and/or PGE2 (0.5 μM) or CGS (50 μM) and/or butaprost (5 μM) for 10 min. The levels of cAMP in cellular extracts were analyzed using mass spectrometry. b, c TILs #2009 at stage II were stimulated with anti-CD3 in the presence of CADO (50 μM) and/or PGE2 (0.5 μM) for 30 min. TIL protein extracts (50 μg) were resolved using 10% SDS-PAGE and transferred to PVDF membranes. The level of CREB (b) and Csk (c) phosphorylation was analyzed using specific antibodies. The optic density (OD) ratio of the bands was analyzed using ImageQuanT data analysis software (Molecular Dynamics)

    Journal: Cancer immunology, immunotherapy : CII

    Article Title: Receptor desensitization and blockade of the suppressive effects of prostaglandin E 2 and adenosine on the cytotoxic activity of human melanoma-infiltrating T lymphocytes

    doi: 10.1007/s00262-010-0924-z

    Figure Lengend Snippet: Effect of CADO and PGE2 on cAMP production (a) and CREB (b) and Csk (c) phosphorylation. a TILs #2009 at stage II were incubated with CADO (50 μM) and/or PGE2 (0.5 μM) or CGS (50 μM) and/or butaprost (5 μM) for 10 min. The levels of cAMP in cellular extracts were analyzed using mass spectrometry. b, c TILs #2009 at stage II were stimulated with anti-CD3 in the presence of CADO (50 μM) and/or PGE2 (0.5 μM) for 30 min. TIL protein extracts (50 μg) were resolved using 10% SDS-PAGE and transferred to PVDF membranes. The level of CREB (b) and Csk (c) phosphorylation was analyzed using specific antibodies. The optic density (OD) ratio of the bands was analyzed using ImageQuanT data analysis software (Molecular Dynamics)

    Article Snippet: Films were scanned and analyzed by ImageQuanT data analysis software (Molecular Dynamics) [ 17 ].

    Techniques: Phospho-proteomics, Incubation, Mass Spectrometry, SDS Page, Software