computerized image analysis program (imagej software)  (MetaMorph Inc)

Journal: Nature

Article Title: Fibroblastic reticular cells direct the initiation of T cell responses via CD44

doi: 10.1038/s41586-025-09988-8

Figure Lengend Snippet: a – d , Numbers of CD8 T cells ( a ), virus-specific IE1-tetramer + CD8 T cells ( b ) and percentage ( c ) and number ( d ) of MPEC (CD127 hi ) and SLEC (CD127 low ) IE1 + virus-specific CD8 T cells in spleens of uninfected mice or mice infected with wild-type or ∆vCD44BP MCMV (7 dpi). Data in a – d are pooled from three independent experiments (naive, n = 6; MCMV and ∆vCD44BP, n = 12). e , Numbers of cDC1s and cDC2s in spleens of uninfected mice or mice infected with MCMV or ∆vCD44BP. Data are pooled from two independent experiments ( n = 8 per group). f , Frequency of IV − dendritic cells in spleens of uninfected mice or mice at 36 h after infection with MCMV or ∆vCD44BP. Data are pooled from two independent experiments (naive, n = 5; MCMV and ∆vCD44BP, n = 8). g , Spleen sections from uninfected mice and mice infected with MCMV or ∆vCD44BP (2 dpi). ImageJ images processed with Imaris (for display purposes only) are shown. Scale bars, 250 μm. WP, white pulp; MZ, marginal zone; MZBC, marginal zone bridging channel. h , Mean CD11c staining intensities in T cell zones at 2 dpi. i , Representative confocal image showing CD8, IE1 and CD11c in the splenic white pulp of MCMV-infected mice (2 dpi). The white circle highlights an area in which MCMV-infected IE1 + cells are in close contact with CD11c + dendritic cells and CD8 + T cells. The image is representative of data from three independent experiments. Scale bar, 20 μm. All mice were infected with 5 × 10 3 PFU. Graphs show mean ± s.e.m.; violin plots show median and quartiles; significance tested by two-sided Mann–Whitney test.

Article Snippet: ImageJ images processed with Imaris (for display purposes only) are shown.

Techniques: Virus, Infection, Staining, MANN-WHITNEY