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imagej software  (Bio-Rad)


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    Structured Review

    Bio-Rad imagej software
    Imagej Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 35689 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagej+images/Image+Lab+Software/pm41895111-76-16-18
    Average 99 stars, based on 35689 article reviews
    imagej software - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    other:

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: After washing and incubating with an appropriate horse radish peroxidase (HRP) secondary antibody (HRP goat anti-mouse 51782504 and HRP goat anti-rabbit 17065156; Bio-Rad Laboratories) and following TBST washes, the membranes were coated with chemiluminescent substrate (West Femto; Thermo Fisher Scientific).

    Software:

    Article Title: Modified tumor uptake and biodistribution of nanoparticles coated with small extracellular vesicle membranes derived from distinct tumor cell lines
    Article Snippet: After washing, protein bands were visualized using SuperSignal West Femto (34096, ThermoFisher) and imaged on ChemiDoc MP imaging system (Bio-Rad). .. The signal intensity of bands were quantified utilizing ImageLab software (Bio-Rad). ..

    Article Title: Alternative splicing of a species- and skeletal muscle-specific microexon diversifies the menu of clathrin heavy chains in mice and rats.
    Article Snippet: PCR products were resolved via 6% polyacrylamide gel electrophoresis using TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA pH 8.5) for 3–4 h at 150–160 V. Polyacrylamide gels were stained with 0.4 μg/mL ethidium bromide for 10 min. All gels were imaged under ultraviolet illumination in a ChemiDoc XRS+ Imaging System (Bio-Rad). .. Alternative splicing was quantified by densitometry using the Image Lab software (version 6.0.1, Bio-Rad). .. The product length-dependent staining bias of ethidium bromide was corrected using a standard curve based upon the pUC19 ladder (Thermo Fisher Scientific, SM0221).

    Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice
    Article Snippet: Blots were covered in Clarity Max Chemiluminescent reagent (Bio-Rad) and visualized using the ChemiDoc XRS+ Imaging System (Bio-Rad). .. Densitometry was analyzed using ImageLab software (V6.0.1; Bio-Rad). .. Immunoblotting was performed with antibodies against lysocardiolipin acyltransferase 1(LCLAT1, PA5-25627; Thermo Fisher Scientific), PGC-1α (ab191838; Abcam, Cambridge, UK), MFN2 (PA5-118059; Thermo Fisher Scientific), ANP (sc-18811; Santa Cruz, Dallas, TX, USA), tumor necrosis factor alpha (TNFα, 3707; Cell Signaling, Danvers, MA, USA), phosphorylated adenosine monophosphate-activated protein kinase (AMPK, 2531; Cell Signaling), AMPKα (2532; Cell Signaling), Perilipin 5 (PA1-46215; Thermo Fisher Scientific), translocase of outer mitochondria membrane 70 (TOM70, 65675; Cell Signaling), cytochrome c oxidase subunit 4 (COXIV, 4844; Cell Signaling), and calnexin (208880; Merck, Rahway, NJ, USA).

    Article Title: Single-cell RNA sequencing identifies a proinflammatory S100a8/a9 + macrophage subset contributing to acute kidney injury.
    Article Snippet: 1 Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China 2 Kidney and Blood Purification Laboratory of Shanghai, Shanghai, China 3 Shanghai Medical Center of Kidney, Shanghai, China 4 Kidney and Dialysis Institute of Shanghai, Shanghai, China 5 Hemodialysis Quality Control Center of Shanghai, Shanghai, China Abstract Objective Macrophages represent a heterogeneous cell population in the kidney.. However, macrophage subsets and their role in endotoxemia-induced acute kidney injury (AKI) remain elucidated.. Material and methods Single-cell RNA sequencing was performed to identify macrophage subpopulations in mouse kidney following lipopolysaccharide (LPS) challenge.

    Article Title: Targeting activated hepatic stellate cells through ferroptosis: phenethyl isothiocyanate attenuates CCl 4 -induced liver fibrosis by regulating iron metabolism, redox homeostasis, and autophagy.
    Article Snippet: Phenethyl isothiocyanate (PEITC), a dietary compound from cruciferous vegetables, is known for its chemopreventive activity, but its effects on liver fibrosis remain largely unexplored.. In this study, we show that PEITC administration attenuated carbon tetrachloride (CCl4)-induced hepatic injury and fibrosis in mice, as evidenced by reduced collagen deposition, hydroxyproline content and fibrotic marker expression, and improved liver function.. PEITC inhibited cell survival and fibrogenic phenotypes in transforming growth factor β (TGF-β)-stimulated LX-2 human hepatic stellate cells (HSCs) while exhibiting minimal cytotoxicity towards normal hepatocytes.

    Article Title: OGT regulates RNA-binding protein nucleocytoplasmic partitioning, but this fails in amyotrophic lateral sclerosis.
    Article Snippet: All membranes were imaged using a ChemiDoc MP imaging system (Bio-Rad). .. Band intensities were analyzed using Image Lab software (Bio-Rad). .. Samples were fixed in 4% paraformaldehyde (Thermo Scientific, 043368.9M) diluted in PBS (Gibco, 10010015) and then blocked in 5% bovine serum albumin (BSA, Sigma-Aldrich, A7906) in PBS, containing 0.3% Triton X-100 (Thermo Scientific, 9002–93-1).

    Alternative Splicing:

    Article Title: Alternative splicing of a species- and skeletal muscle-specific microexon diversifies the menu of clathrin heavy chains in mice and rats.
    Article Snippet: PCR products were resolved via 6% polyacrylamide gel electrophoresis using TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA pH 8.5) for 3–4 h at 150–160 V. Polyacrylamide gels were stained with 0.4 μg/mL ethidium bromide for 10 min. All gels were imaged under ultraviolet illumination in a ChemiDoc XRS+ Imaging System (Bio-Rad). .. Alternative splicing was quantified by densitometry using the Image Lab software (version 6.0.1, Bio-Rad). .. The product length-dependent staining bias of ethidium bromide was corrected using a standard curve based upon the pUC19 ladder (Thermo Fisher Scientific, SM0221).

    Incubation:

    Article Title: Targeting activated hepatic stellate cells through ferroptosis: phenethyl isothiocyanate attenuates CCl 4 -induced liver fibrosis by regulating iron metabolism, redox homeostasis, and autophagy.
    Article Snippet: Phenethyl isothiocyanate (PEITC), a dietary compound from cruciferous vegetables, is known for its chemopreventive activity, but its effects on liver fibrosis remain largely unexplored.. In this study, we show that PEITC administration attenuated carbon tetrachloride (CCl4)-induced hepatic injury and fibrosis in mice, as evidenced by reduced collagen deposition, hydroxyproline content and fibrotic marker expression, and improved liver function.. PEITC inhibited cell survival and fibrogenic phenotypes in transforming growth factor β (TGF-β)-stimulated LX-2 human hepatic stellate cells (HSCs) while exhibiting minimal cytotoxicity towards normal hepatocytes.

    Automatic:

    Article Title: Targeting activated hepatic stellate cells through ferroptosis: phenethyl isothiocyanate attenuates CCl 4 -induced liver fibrosis by regulating iron metabolism, redox homeostasis, and autophagy.
    Article Snippet: Phenethyl isothiocyanate (PEITC), a dietary compound from cruciferous vegetables, is known for its chemopreventive activity, but its effects on liver fibrosis remain largely unexplored.. In this study, we show that PEITC administration attenuated carbon tetrachloride (CCl4)-induced hepatic injury and fibrosis in mice, as evidenced by reduced collagen deposition, hydroxyproline content and fibrotic marker expression, and improved liver function.. PEITC inhibited cell survival and fibrogenic phenotypes in transforming growth factor β (TGF-β)-stimulated LX-2 human hepatic stellate cells (HSCs) while exhibiting minimal cytotoxicity towards normal hepatocytes.

    Fluorescence:

    Article Title: Targeting activated hepatic stellate cells through ferroptosis: phenethyl isothiocyanate attenuates CCl 4 -induced liver fibrosis by regulating iron metabolism, redox homeostasis, and autophagy.
    Article Snippet: Phenethyl isothiocyanate (PEITC), a dietary compound from cruciferous vegetables, is known for its chemopreventive activity, but its effects on liver fibrosis remain largely unexplored.. In this study, we show that PEITC administration attenuated carbon tetrachloride (CCl4)-induced hepatic injury and fibrosis in mice, as evidenced by reduced collagen deposition, hydroxyproline content and fibrotic marker expression, and improved liver function.. PEITC inhibited cell survival and fibrogenic phenotypes in transforming growth factor β (TGF-β)-stimulated LX-2 human hepatic stellate cells (HSCs) while exhibiting minimal cytotoxicity towards normal hepatocytes.

    Imaging:

    Article Title: Targeting activated hepatic stellate cells through ferroptosis: phenethyl isothiocyanate attenuates CCl 4 -induced liver fibrosis by regulating iron metabolism, redox homeostasis, and autophagy.
    Article Snippet: Phenethyl isothiocyanate (PEITC), a dietary compound from cruciferous vegetables, is known for its chemopreventive activity, but its effects on liver fibrosis remain largely unexplored.. In this study, we show that PEITC administration attenuated carbon tetrachloride (CCl4)-induced hepatic injury and fibrosis in mice, as evidenced by reduced collagen deposition, hydroxyproline content and fibrotic marker expression, and improved liver function.. PEITC inhibited cell survival and fibrogenic phenotypes in transforming growth factor β (TGF-β)-stimulated LX-2 human hepatic stellate cells (HSCs) while exhibiting minimal cytotoxicity towards normal hepatocytes.

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: After a series of steps involving drying, re-activating, washing, and blocking, membranes were incubated in antibodies (for full details, see Latchman et al., ): firstly, anti-myosin heavy chain IIa (MHCIIa) (mouse, monoclonal IgG, A4.74; Developmental Studies Hybridoma Bank (DSHB), Iowa city, IA, USA), followed by stripping and then anti-MHCI (mouse monoclonal IgM, A4.840; DSHB). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories).



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    a – d , Numbers of CD8 T cells ( a ), virus-specific IE1-tetramer + CD8 T cells ( b ) and percentage ( c ) and number ( d ) of MPEC (CD127 hi ) and SLEC (CD127 low ) IE1 + virus-specific CD8 T cells in spleens of uninfected mice or mice infected with wild-type or ∆vCD44BP MCMV (7 dpi). Data in a – d are pooled from three independent experiments (naive, n = 6; MCMV and ∆vCD44BP, n = 12). e , Numbers of cDC1s and cDC2s in spleens of uninfected mice or mice infected with MCMV or ∆vCD44BP. Data are pooled from two independent experiments ( n = 8 per group). f , Frequency of IV − dendritic cells in spleens of uninfected mice or mice at 36 h after infection with MCMV or ∆vCD44BP. Data are pooled from two independent experiments (naive, n = 5; MCMV and ∆vCD44BP, n = 8). g , Spleen sections from uninfected mice and mice infected with MCMV or ∆vCD44BP (2 dpi). <t>ImageJ</t> images processed <t>with</t> <t>Imaris</t> (for display purposes only) are shown. Scale bars, 250 μm. WP, white pulp; MZ, marginal zone; MZBC, marginal zone bridging channel. h , Mean CD11c staining intensities in T cell zones at 2 dpi. i , Representative confocal image showing CD8, IE1 and CD11c in the splenic white pulp of MCMV-infected mice (2 dpi). The white circle highlights an area in which MCMV-infected IE1 + cells are in close contact with CD11c + dendritic cells and CD8 + T cells. The image is representative of data from three independent experiments. Scale bar, 20 μm. All mice were infected with 5 × 10 3 PFU. Graphs show mean ± s.e.m.; violin plots show median and quartiles; significance tested by two-sided Mann–Whitney test.
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    Image Search Results


    a – d , Numbers of CD8 T cells ( a ), virus-specific IE1-tetramer + CD8 T cells ( b ) and percentage ( c ) and number ( d ) of MPEC (CD127 hi ) and SLEC (CD127 low ) IE1 + virus-specific CD8 T cells in spleens of uninfected mice or mice infected with wild-type or ∆vCD44BP MCMV (7 dpi). Data in a – d are pooled from three independent experiments (naive, n = 6; MCMV and ∆vCD44BP, n = 12). e , Numbers of cDC1s and cDC2s in spleens of uninfected mice or mice infected with MCMV or ∆vCD44BP. Data are pooled from two independent experiments ( n = 8 per group). f , Frequency of IV − dendritic cells in spleens of uninfected mice or mice at 36 h after infection with MCMV or ∆vCD44BP. Data are pooled from two independent experiments (naive, n = 5; MCMV and ∆vCD44BP, n = 8). g , Spleen sections from uninfected mice and mice infected with MCMV or ∆vCD44BP (2 dpi). ImageJ images processed with Imaris (for display purposes only) are shown. Scale bars, 250 μm. WP, white pulp; MZ, marginal zone; MZBC, marginal zone bridging channel. h , Mean CD11c staining intensities in T cell zones at 2 dpi. i , Representative confocal image showing CD8, IE1 and CD11c in the splenic white pulp of MCMV-infected mice (2 dpi). The white circle highlights an area in which MCMV-infected IE1 + cells are in close contact with CD11c + dendritic cells and CD8 + T cells. The image is representative of data from three independent experiments. Scale bar, 20 μm. All mice were infected with 5 × 10 3 PFU. Graphs show mean ± s.e.m.; violin plots show median and quartiles; significance tested by two-sided Mann–Whitney test.

    Journal: Nature

    Article Title: Fibroblastic reticular cells direct the initiation of T cell responses via CD44

    doi: 10.1038/s41586-025-09988-8

    Figure Lengend Snippet: a – d , Numbers of CD8 T cells ( a ), virus-specific IE1-tetramer + CD8 T cells ( b ) and percentage ( c ) and number ( d ) of MPEC (CD127 hi ) and SLEC (CD127 low ) IE1 + virus-specific CD8 T cells in spleens of uninfected mice or mice infected with wild-type or ∆vCD44BP MCMV (7 dpi). Data in a – d are pooled from three independent experiments (naive, n = 6; MCMV and ∆vCD44BP, n = 12). e , Numbers of cDC1s and cDC2s in spleens of uninfected mice or mice infected with MCMV or ∆vCD44BP. Data are pooled from two independent experiments ( n = 8 per group). f , Frequency of IV − dendritic cells in spleens of uninfected mice or mice at 36 h after infection with MCMV or ∆vCD44BP. Data are pooled from two independent experiments (naive, n = 5; MCMV and ∆vCD44BP, n = 8). g , Spleen sections from uninfected mice and mice infected with MCMV or ∆vCD44BP (2 dpi). ImageJ images processed with Imaris (for display purposes only) are shown. Scale bars, 250 μm. WP, white pulp; MZ, marginal zone; MZBC, marginal zone bridging channel. h , Mean CD11c staining intensities in T cell zones at 2 dpi. i , Representative confocal image showing CD8, IE1 and CD11c in the splenic white pulp of MCMV-infected mice (2 dpi). The white circle highlights an area in which MCMV-infected IE1 + cells are in close contact with CD11c + dendritic cells and CD8 + T cells. The image is representative of data from three independent experiments. Scale bar, 20 μm. All mice were infected with 5 × 10 3 PFU. Graphs show mean ± s.e.m.; violin plots show median and quartiles; significance tested by two-sided Mann–Whitney test.

    Article Snippet: ImageJ images processed with Imaris (for display purposes only) are shown.

    Techniques: Virus, Infection, Staining, MANN-WHITNEY