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axiovision image analysis software zen2  (Carl Zeiss)


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    Structured Review

    Carl Zeiss axiovision image analysis software zen2
    Axiovision Image Analysis Software Zen2, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 95/100, based on 196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image+analysis/ZEN+Module+Image+Analysis/pm42853007-162-5-10
    Average 95 stars, based on 196 article reviews
    axiovision image analysis software zen2 - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    Analysis:

    Article Title: ACL injury reprograms quadriceps myonuclear epigenetic and transcriptional signatures in mice
    Article Snippet: Immunofluorescence was visualized at 10 × or 20 × magnification (Plan-Apochromat 10x, NA: 0.45, air; Plan-Apochromat 20x, NA: 0.8, air) using a Zeiss M2 Axioimager with an automatic stage (Zeiss, Oberkochen, Germany). .. Image analysis for EU intensity and RNA-FISH was performed by a single assessor (TC or SGV) using Zeiss Zen software (v3.1), blinded to group allocation and injury status of the muscle. ..

    Article Title: Photobiomodulation Triggers M1/M2 Macrophage Polarization in Zymosan-Induced Acute Arthritis in Mice.
    Article Snippet: Objective: To investigate whether photobiomodulation (PBM) modulates macrophage polarization in zymosaninduced acute joint inflammation, promoting an M1 to M2 phenotype shift.. Methods: Mice received intraarticular zymosan and PBM (830 nm, 10 mW) at 3 and 30 J/cm2.. Euthanasia occurred at 24, 48, and 72 h. Polarization was assessed via mRNA levels and protein expression.

    Article Title: Spontaneous Achromatopsia-Like Phenotype in Albino Swiss CD-1 Mice: Retinocortical Dysfunction, Neurovascular Remodeling, and Glial Activation.
    Article Snippet: It also features the following filter sets: a Zeiss 10 filter set for Alexa Fluor 488, a Zeiss 64 filter set for Alexa Fluor 594, and a Zeiss 49 filter set for Alexa Fluor 405. .. The equipment is fitted with AxioVision image analysis software ZEN2 (Carl Zeiss AG), which was used for capturing and analyzing the images, ensuring that lighting conditions and magnifications were consistently maintained throughout the imaging process. ..

    Article Title: Complex interplay of neuronal and hormonal gut-brain responses to essential amino acid deficit.
    Article Snippet: INTRODUCTION: Animals maintain nutrient homeostasis by adjusting feeding behavior according to internal nutritional needs.. Protein intake is particularly critical because essential amino acids (EAAs) cannot be synthesized de novo and must be obtained from the diet.. When dietary protein becomes limiting, animals develop a compensatory appetite that prioritizes proteinrich foods or EAAcontaining foods.

    Article Title: The endo-lysosomal system drives lumen formation in a human epiblast model.
    Article Snippet: Surface rendering feature in Imaris (Oxford Instruments) was used to generate 3D surfaces (1.0μm surface level) and to measure lumenal sphericity in Fig. 10E. .. Lumenal circularity and roundness in Fig. 10F, G were measured from all optical sections with an open central lumen labeled by PODXL using Image Analysis module in ZEN (Zeiss). ..

    Article Title: Neurotensin stimulates androgen production by theca cells in the monkey ovarian follicle
    Article Snippet: Cells were washed, counterstained with Hoechst 33342, and visualized via fluorescence inverted microscopy (ZEISS, Oberkochen, Germany). .. Three fields were imaged per treatment, and pHrodo-Green fluorescent area was calculated per Hoechst-stained nuclei count using ZEN Blue software image analysis (Zeiss). ..

    Software:

    Article Title: ACL injury reprograms quadriceps myonuclear epigenetic and transcriptional signatures in mice
    Article Snippet: Immunofluorescence was visualized at 10 × or 20 × magnification (Plan-Apochromat 10x, NA: 0.45, air; Plan-Apochromat 20x, NA: 0.8, air) using a Zeiss M2 Axioimager with an automatic stage (Zeiss, Oberkochen, Germany). .. Image analysis for EU intensity and RNA-FISH was performed by a single assessor (TC or SGV) using Zeiss Zen software (v3.1), blinded to group allocation and injury status of the muscle. ..

    Article Title: Temporal variations in microplastic- associated oxidative stress and fillet quality of whiting (Merlangius merlangus) and potential human intake: A multi-year field study.
    Article Snippet: While global microplastic (MPs) pollution threatens aquatic ecosystems and food safety, the widespread presence of MP sin marine fish and the associated health risks have raised significant concerns.. This study aims to detect/ characterize the presence of MP in whiting (Merlangius merlangus) fish caught in different regions of the Black Sea over two years on a seasonal basis, to determine the toxicity potential in fish (biochemical, histopathological, and immunohistochemical), fillet quality (protein oxidation), and the effects on potential MPs intake levels in humans during 2 years.. MPs were detected across all sampling regions, seasons, and examined tissues, although their occurrence and abundance varied according to year, season, and region, with relatively higher MP burdens observed in samples from the Western and Eastern Black Sea.

    Article Title: Photobiomodulation Triggers M1/M2 Macrophage Polarization in Zymosan-Induced Acute Arthritis in Mice.
    Article Snippet: Objective: To investigate whether photobiomodulation (PBM) modulates macrophage polarization in zymosaninduced acute joint inflammation, promoting an M1 to M2 phenotype shift.. Methods: Mice received intraarticular zymosan and PBM (830 nm, 10 mW) at 3 and 30 J/cm2.. Euthanasia occurred at 24, 48, and 72 h. Polarization was assessed via mRNA levels and protein expression.

    Article Title: Spontaneous Achromatopsia-Like Phenotype in Albino Swiss CD-1 Mice: Retinocortical Dysfunction, Neurovascular Remodeling, and Glial Activation.
    Article Snippet: It also features the following filter sets: a Zeiss 10 filter set for Alexa Fluor 488, a Zeiss 64 filter set for Alexa Fluor 594, and a Zeiss 49 filter set for Alexa Fluor 405. .. The equipment is fitted with AxioVision image analysis software ZEN2 (Carl Zeiss AG), which was used for capturing and analyzing the images, ensuring that lighting conditions and magnifications were consistently maintained throughout the imaging process. ..

    Article Title: Complex interplay of neuronal and hormonal gut-brain responses to essential amino acid deficit.
    Article Snippet: INTRODUCTION: Animals maintain nutrient homeostasis by adjusting feeding behavior according to internal nutritional needs.. Protein intake is particularly critical because essential amino acids (EAAs) cannot be synthesized de novo and must be obtained from the diet.. When dietary protein becomes limiting, animals develop a compensatory appetite that prioritizes proteinrich foods or EAAcontaining foods.

    Article Title: Mucoadhesive Chitosome-Based Buccal Delivery of Goat Placenta-Derived Bioactive Macromolecules: Effects on 5-Fluorouracil-Induced Gingival Fibroblast Injury
    Article Snippet: .. Fluorescence distribution throughout the tissue was semi-quantitatively analyzed using ZEISS ZEN software (version 2.3 SP1), which generated fluorescence intensity profiles along the central horizontal axis of each image. ..

    Article Title: Neurotensin stimulates androgen production by theca cells in the monkey ovarian follicle
    Article Snippet: Cells were washed, counterstained with Hoechst 33342, and visualized via fluorescence inverted microscopy (ZEISS, Oberkochen, Germany). .. Three fields were imaged per treatment, and pHrodo-Green fluorescent area was calculated per Hoechst-stained nuclei count using ZEN Blue software image analysis (Zeiss). ..

    Staining:

    Article Title: Temporal variations in microplastic- associated oxidative stress and fillet quality of whiting (Merlangius merlangus) and potential human intake: A multi-year field study.
    Article Snippet: While global microplastic (MPs) pollution threatens aquatic ecosystems and food safety, the widespread presence of MP sin marine fish and the associated health risks have raised significant concerns.. This study aims to detect/ characterize the presence of MP in whiting (Merlangius merlangus) fish caught in different regions of the Black Sea over two years on a seasonal basis, to determine the toxicity potential in fish (biochemical, histopathological, and immunohistochemical), fillet quality (protein oxidation), and the effects on potential MPs intake levels in humans during 2 years.. MPs were detected across all sampling regions, seasons, and examined tissues, although their occurrence and abundance varied according to year, season, and region, with relatively higher MP burdens observed in samples from the Western and Eastern Black Sea.

    Immunohistochemical staining:

    Article Title: Temporal variations in microplastic- associated oxidative stress and fillet quality of whiting (Merlangius merlangus) and potential human intake: A multi-year field study.
    Article Snippet: While global microplastic (MPs) pollution threatens aquatic ecosystems and food safety, the widespread presence of MP sin marine fish and the associated health risks have raised significant concerns.. This study aims to detect/ characterize the presence of MP in whiting (Merlangius merlangus) fish caught in different regions of the Black Sea over two years on a seasonal basis, to determine the toxicity potential in fish (biochemical, histopathological, and immunohistochemical), fillet quality (protein oxidation), and the effects on potential MPs intake levels in humans during 2 years.. MPs were detected across all sampling regions, seasons, and examined tissues, although their occurrence and abundance varied according to year, season, and region, with relatively higher MP burdens observed in samples from the Western and Eastern Black Sea.

    Imaging:

    Article Title: Temporal variations in microplastic- associated oxidative stress and fillet quality of whiting (Merlangius merlangus) and potential human intake: A multi-year field study.
    Article Snippet: While global microplastic (MPs) pollution threatens aquatic ecosystems and food safety, the widespread presence of MP sin marine fish and the associated health risks have raised significant concerns.. This study aims to detect/ characterize the presence of MP in whiting (Merlangius merlangus) fish caught in different regions of the Black Sea over two years on a seasonal basis, to determine the toxicity potential in fish (biochemical, histopathological, and immunohistochemical), fillet quality (protein oxidation), and the effects on potential MPs intake levels in humans during 2 years.. MPs were detected across all sampling regions, seasons, and examined tissues, although their occurrence and abundance varied according to year, season, and region, with relatively higher MP burdens observed in samples from the Western and Eastern Black Sea.

    Article Title: Spontaneous Achromatopsia-Like Phenotype in Albino Swiss CD-1 Mice: Retinocortical Dysfunction, Neurovascular Remodeling, and Glial Activation.
    Article Snippet: It also features the following filter sets: a Zeiss 10 filter set for Alexa Fluor 488, a Zeiss 64 filter set for Alexa Fluor 594, and a Zeiss 49 filter set for Alexa Fluor 405. .. The equipment is fitted with AxioVision image analysis software ZEN2 (Carl Zeiss AG), which was used for capturing and analyzing the images, ensuring that lighting conditions and magnifications were consistently maintained throughout the imaging process. ..

    Labeling:

    Article Title: The endo-lysosomal system drives lumen formation in a human epiblast model.
    Article Snippet: Surface rendering feature in Imaris (Oxford Instruments) was used to generate 3D surfaces (1.0μm surface level) and to measure lumenal sphericity in Fig. 10E. .. Lumenal circularity and roundness in Fig. 10F, G were measured from all optical sections with an open central lumen labeled by PODXL using Image Analysis module in ZEN (Zeiss). ..

    Fluorescence:

    Article Title: Mucoadhesive Chitosome-Based Buccal Delivery of Goat Placenta-Derived Bioactive Macromolecules: Effects on 5-Fluorouracil-Induced Gingival Fibroblast Injury
    Article Snippet: .. Fluorescence distribution throughout the tissue was semi-quantitatively analyzed using ZEISS ZEN software (version 2.3 SP1), which generated fluorescence intensity profiles along the central horizontal axis of each image. ..

    Tissue:

    Article Title: Mucoadhesive Chitosome-Based Buccal Delivery of Goat Placenta-Derived Bioactive Macromolecules: Effects on 5-Fluorouracil-Induced Gingival Fibroblast Injury
    Article Snippet: .. Fluorescence distribution throughout the tissue was semi-quantitatively analyzed using ZEISS ZEN software (version 2.3 SP1), which generated fluorescence intensity profiles along the central horizontal axis of each image. ..

    Generated:

    Article Title: Mucoadhesive Chitosome-Based Buccal Delivery of Goat Placenta-Derived Bioactive Macromolecules: Effects on 5-Fluorouracil-Induced Gingival Fibroblast Injury
    Article Snippet: .. Fluorescence distribution throughout the tissue was semi-quantitatively analyzed using ZEISS ZEN software (version 2.3 SP1), which generated fluorescence intensity profiles along the central horizontal axis of each image. ..



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    Image Search Results


    (A) Detection of SARS-CoV-2 infected cells using high-content imaging under control conditions. Fluorescent signals: green (anti-SARS-CoV NP mAb), blue (Hoechst). (B) Representative flow cytometry analysis plots showing NP+ cells. (C) Measurement of fluorescent intensity in NP+ cells. (D) Percentage of infected MSCs. (E) The supernatants of infected MSCs were collected and titrated on Vero-E6/TMPRSS2 cells to measure infectious viral particle production using the focus-forming assay. (F) After RNA extraction from infected MSCs, the expression of IDO1 was examined using real-time PCR. The graph displays the expression of IDO1 normalized to GAPDH , with the mock group serving as the control. Data are represented as means ± SEM from three independent biological replicates. For , the Delta condition was available from only two independent donors and was therefore presented descriptively and excluded from the statistical analysis. Statistical comparisons among the remaining groups were performed using one-way ANOVA followed by Tukey’s multiple-comparisons test. One-way ANOVA showed a significant overall difference among groups, F(2, 6) = 6.794, P = 0.0287. * P < 0.05, ** P < 0.01, *** P = 0.0001, **** P < 0.0001.

    Journal: PLOS One

    Article Title: Omicron SARS-CoV-2 shows NRP1-linked susceptibility in adipose-derived MSCs and alters primitive cord blood CD34⁺ HSPCs

    doi: 10.1371/journal.pone.0360439

    Figure Lengend Snippet: (A) Detection of SARS-CoV-2 infected cells using high-content imaging under control conditions. Fluorescent signals: green (anti-SARS-CoV NP mAb), blue (Hoechst). (B) Representative flow cytometry analysis plots showing NP+ cells. (C) Measurement of fluorescent intensity in NP+ cells. (D) Percentage of infected MSCs. (E) The supernatants of infected MSCs were collected and titrated on Vero-E6/TMPRSS2 cells to measure infectious viral particle production using the focus-forming assay. (F) After RNA extraction from infected MSCs, the expression of IDO1 was examined using real-time PCR. The graph displays the expression of IDO1 normalized to GAPDH , with the mock group serving as the control. Data are represented as means ± SEM from three independent biological replicates. For , the Delta condition was available from only two independent donors and was therefore presented descriptively and excluded from the statistical analysis. Statistical comparisons among the remaining groups were performed using one-way ANOVA followed by Tukey’s multiple-comparisons test. One-way ANOVA showed a significant overall difference among groups, F(2, 6) = 6.794, P = 0.0287. * P < 0.05, ** P < 0.01, *** P = 0.0001, **** P < 0.0001.

    Article Snippet: The Operetta high-content imaging system (PerkinElmer) was used to detect and analyze the fluorescent signals.

    Techniques: Infection, Imaging, Control, Flow Cytometry, Analysis, Focus Forming Assay, RNA Extraction, Expressing, Real-time Polymerase Chain Reaction

    (A) Representative western blot depicting the expression of NRP1 in AD-MSCs (No siRNA), cells transfected with non-targeting siRNA (siControl), or siNRP1 for 24 hours. β-actin was used as a loading control. (B) Graph displaying the quantification of NRP1 normalized to β-actin (n = 3). This experiment represents three independent biological replications. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test. (C) Transfected cells were visualized using high-content imaging. Fluorescent signals: green for anti-NRP1 mAb, blue for Hoechst. (D) Percentage of NRP1-expressing cells normalized to cells transfected with siControl. Data are presented as mean ± SEM from three independent experiments. Statistical analysis was conducted using a paired t-test. Next, siRNA-transfected cells were infected with different variants of SARS-CoV-2. (E) SARS-CoV-2-infected cells were detected using high-content imaging. Fluorescent signals: green for anti-SARS-CoV NP mAb, blue for Hoechst. (F) The fluorescent intensity of NP+ cells was measured. All data represent three independent experiments. Statistical analysis among each group of variant was performed using an unpaired t-test. * P < 0.05, ** P < 0.01, *** P = 0.0001, **** P < 0.0001.

    Journal: PLOS One

    Article Title: Omicron SARS-CoV-2 shows NRP1-linked susceptibility in adipose-derived MSCs and alters primitive cord blood CD34⁺ HSPCs

    doi: 10.1371/journal.pone.0360439

    Figure Lengend Snippet: (A) Representative western blot depicting the expression of NRP1 in AD-MSCs (No siRNA), cells transfected with non-targeting siRNA (siControl), or siNRP1 for 24 hours. β-actin was used as a loading control. (B) Graph displaying the quantification of NRP1 normalized to β-actin (n = 3). This experiment represents three independent biological replications. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test. (C) Transfected cells were visualized using high-content imaging. Fluorescent signals: green for anti-NRP1 mAb, blue for Hoechst. (D) Percentage of NRP1-expressing cells normalized to cells transfected with siControl. Data are presented as mean ± SEM from three independent experiments. Statistical analysis was conducted using a paired t-test. Next, siRNA-transfected cells were infected with different variants of SARS-CoV-2. (E) SARS-CoV-2-infected cells were detected using high-content imaging. Fluorescent signals: green for anti-SARS-CoV NP mAb, blue for Hoechst. (F) The fluorescent intensity of NP+ cells was measured. All data represent three independent experiments. Statistical analysis among each group of variant was performed using an unpaired t-test. * P < 0.05, ** P < 0.01, *** P = 0.0001, **** P < 0.0001.

    Article Snippet: The Operetta high-content imaging system (PerkinElmer) was used to detect and analyze the fluorescent signals.

    Techniques: Western Blot, Expressing, Transfection, Control, Analysis, Imaging, Infection, Variant Assay