Review



incucyte live cell imaging system  (Sartorius AG)


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    Structured Review

    Sartorius AG incucyte live cell imaging system
    ART6043 potentiates PARP inhibition in vitro . A, Representative CTG assay combination matrix for DLD-1 BRCA2 −/− (left) and DLD-1 wild-type (right) cells treated with titrated ART6043 and talazoparib for 14 days. Intensity of blue color indicates the level of synergy. % Control indicates relative viability. B, Representative <t>IncuCyte</t> growth curves for DLD-1 BRCA2 −/− cells treated with ART6043 or talazoparib as monotherapy or in combination at the indicated doses for 14 days. C, As for A but using olaparib instead of talazoparib. D, As for B but using olaparib instead of talazoparib. ART6043 concentrations represent free drug in tissue culture medium.
    Incucyte Live Cell Imaging System, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 16104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image+analysis/Live+Cell+Analysis+Instruments/pmc13628093-108-5-9
    Average 99 stars, based on 16104 article reviews
    incucyte live cell imaging system - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "The Pharmacologic Profile of ART6043, a First-in-Class Clinical DNA Polymerase Theta Polymerase Domain Inhibitor Potentiating PARP Inhibitor Efficacy"

    Article Title: The Pharmacologic Profile of ART6043, a First-in-Class Clinical DNA Polymerase Theta Polymerase Domain Inhibitor Potentiating PARP Inhibitor Efficacy

    Journal: Clinical Cancer Research

    doi: 10.1158/1078-0432.CCR-26-0493

    ART6043 potentiates PARP inhibition in vitro . A, Representative CTG assay combination matrix for DLD-1 BRCA2 −/− (left) and DLD-1 wild-type (right) cells treated with titrated ART6043 and talazoparib for 14 days. Intensity of blue color indicates the level of synergy. % Control indicates relative viability. B, Representative IncuCyte growth curves for DLD-1 BRCA2 −/− cells treated with ART6043 or talazoparib as monotherapy or in combination at the indicated doses for 14 days. C, As for A but using olaparib instead of talazoparib. D, As for B but using olaparib instead of talazoparib. ART6043 concentrations represent free drug in tissue culture medium.
    Figure Legend Snippet: ART6043 potentiates PARP inhibition in vitro . A, Representative CTG assay combination matrix for DLD-1 BRCA2 −/− (left) and DLD-1 wild-type (right) cells treated with titrated ART6043 and talazoparib for 14 days. Intensity of blue color indicates the level of synergy. % Control indicates relative viability. B, Representative IncuCyte growth curves for DLD-1 BRCA2 −/− cells treated with ART6043 or talazoparib as monotherapy or in combination at the indicated doses for 14 days. C, As for A but using olaparib instead of talazoparib. D, As for B but using olaparib instead of talazoparib. ART6043 concentrations represent free drug in tissue culture medium.

    Techniques Used: Inhibition, In Vitro, CTG Assay, Control, Tissue

    Related Articles

    Software:

    Article Title: Species-specific inflammatory responses of chondrocytes: Toward evidence-based model selection in OA research
    Article Snippet: .. IncuCyte software analysis , Sartorius , RRID:SCR_003935; https://www.sartorius.com/en/product. ..

    Article Title: Targeting CD28 on T lineage malignancies with chimeric antigen receptor T cells.
    Article Snippet: .. Quantification of cellular abundance was performed as recommended by measuring GFP integrated intensity in at least technical triplicates (Sartorius, Incucyte Software Version 2023A-2025B). ..

    Article Title: Astrocytes regulate axonal mitochondrial transport deficits in C9ORF72 amyotrophic lateral sclerosis motor neurons.
    Article Snippet: .. Software and code Policy information about availability of computer code Data collection Zeiss Zen software (Carl Zeiss), Seahorse Wave Desktop Software v2.6 (Agilent Technologies), Harmony software v4.9 (PerkinElmer), IncuCyte software (Sartorius), and ImageJ/Fiji v2.14. .. Data analysis GraphPad Prism v10, R v4.3.2 (including lme4, car, emmeans and DESeq2 packages), and ImageJ/Fiji v2.14 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers.

    Analysis:

    Article Title: Species-specific inflammatory responses of chondrocytes: Toward evidence-based model selection in OA research
    Article Snippet: .. IncuCyte software analysis , Sartorius , RRID:SCR_003935; https://www.sartorius.com/en/product. ..

    Article Title: tRNA dosage regulates lineage dependency and resistance in prostate cancer.
    Article Snippet: .. Cell growth was measured for 7 days using an Incucyte live-cell imaging and analysis system (Sartorius). ..

    Article Title: Differential effects of propranolol enantiomers on haemangioma-derived endothelial spheroids
    Article Snippet: The spheroids were embedded in Cultrex Reduced Growth Factor Basement Membrane Extract (R&D Systems, NE Minneapolis, MN, USA) at a concentration of 4–5 mg/mL, following removal of growth medium and incubated at 37 °C to allow polymerisation. .. Culture media was added on top and whole spheroid area and the sprouting area was monitored over 3 days with the Incucyte Live-Cell Analysis (Sartorius, Essen Bio, Ann Arbor, MI, USA) with scans performed every 24 h using a 4 × objective. ..

    Cell Characterization:

    Article Title: Targeting YAP/TEAD signaling disturbs RNA Pol II activity and enhances immunotherapy response via activated cytosolic DNA sensing pathway in gastroesophageal cancer
    Article Snippet: Cells were cultured in RPMI1640 (Invitrogen) or DMEM (Invitrogen) and supplemented with 10% fetal bovine serum (FBS) (Invitrogen), 100 U/ml penicillin, and 100 mg/ml streptomycin (Invitrogen), and maintained at 37°C in a humidified incubator of 5% CO2. .. Live-Cell proliferation was measured using the IncuCyte cell count proliferation assay (Sartorius). ..

    Proliferation Assay:

    Article Title: Targeting YAP/TEAD signaling disturbs RNA Pol II activity and enhances immunotherapy response via activated cytosolic DNA sensing pathway in gastroesophageal cancer
    Article Snippet: Cells were cultured in RPMI1640 (Invitrogen) or DMEM (Invitrogen) and supplemented with 10% fetal bovine serum (FBS) (Invitrogen), 100 U/ml penicillin, and 100 mg/ml streptomycin (Invitrogen), and maintained at 37°C in a humidified incubator of 5% CO2. .. Live-Cell proliferation was measured using the IncuCyte cell count proliferation assay (Sartorius). ..

    Live Cell Imaging:

    Article Title: tRNA dosage regulates lineage dependency and resistance in prostate cancer.
    Article Snippet: .. Cell growth was measured for 7 days using an Incucyte live-cell imaging and analysis system (Sartorius). ..

    Staining:

    Article Title: Gasdermin D-mediated delivery of caspase inhibitors to suppress pyroptosis.
    Article Snippet: Caspase-1, -4, -5 and -11 activate gasdermin D (GSDMD) pores, causing pyroptotic cell death and the release of the interleukins IL-1β and IL-18 (ref. 1).. Blocking this pathway holds therapeutic promise for the treatment of inflammatory disorders, but cellpermeable caspase inhibitors have not proved successful in clinical trials.. Here we describe covalent caspase inhibitors that selectively block pyroptosis and IL-1β secretion despite being excluded from healthy cells.



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    Image Search Results


    (A) Detection of SARS-CoV-2 infected cells using high-content imaging under control conditions. Fluorescent signals: green (anti-SARS-CoV NP mAb), blue (Hoechst). (B) Representative flow cytometry analysis plots showing NP+ cells. (C) Measurement of fluorescent intensity in NP+ cells. (D) Percentage of infected MSCs. (E) The supernatants of infected MSCs were collected and titrated on Vero-E6/TMPRSS2 cells to measure infectious viral particle production using the focus-forming assay. (F) After RNA extraction from infected MSCs, the expression of IDO1 was examined using real-time PCR. The graph displays the expression of IDO1 normalized to GAPDH , with the mock group serving as the control. Data are represented as means ± SEM from three independent biological replicates. For , the Delta condition was available from only two independent donors and was therefore presented descriptively and excluded from the statistical analysis. Statistical comparisons among the remaining groups were performed using one-way ANOVA followed by Tukey’s multiple-comparisons test. One-way ANOVA showed a significant overall difference among groups, F(2, 6) = 6.794, P = 0.0287. * P < 0.05, ** P < 0.01, *** P = 0.0001, **** P < 0.0001.

    Journal: PLOS One

    Article Title: Omicron SARS-CoV-2 shows NRP1-linked susceptibility in adipose-derived MSCs and alters primitive cord blood CD34⁺ HSPCs

    doi: 10.1371/journal.pone.0360439

    Figure Lengend Snippet: (A) Detection of SARS-CoV-2 infected cells using high-content imaging under control conditions. Fluorescent signals: green (anti-SARS-CoV NP mAb), blue (Hoechst). (B) Representative flow cytometry analysis plots showing NP+ cells. (C) Measurement of fluorescent intensity in NP+ cells. (D) Percentage of infected MSCs. (E) The supernatants of infected MSCs were collected and titrated on Vero-E6/TMPRSS2 cells to measure infectious viral particle production using the focus-forming assay. (F) After RNA extraction from infected MSCs, the expression of IDO1 was examined using real-time PCR. The graph displays the expression of IDO1 normalized to GAPDH , with the mock group serving as the control. Data are represented as means ± SEM from three independent biological replicates. For , the Delta condition was available from only two independent donors and was therefore presented descriptively and excluded from the statistical analysis. Statistical comparisons among the remaining groups were performed using one-way ANOVA followed by Tukey’s multiple-comparisons test. One-way ANOVA showed a significant overall difference among groups, F(2, 6) = 6.794, P = 0.0287. * P < 0.05, ** P < 0.01, *** P = 0.0001, **** P < 0.0001.

    Article Snippet: The Operetta high-content imaging system (PerkinElmer) was used to detect and analyze the fluorescent signals.

    Techniques: Infection, Imaging, Control, Flow Cytometry, Analysis, Focus Forming Assay, RNA Extraction, Expressing, Real-time Polymerase Chain Reaction

    (A) Representative western blot depicting the expression of NRP1 in AD-MSCs (No siRNA), cells transfected with non-targeting siRNA (siControl), or siNRP1 for 24 hours. β-actin was used as a loading control. (B) Graph displaying the quantification of NRP1 normalized to β-actin (n = 3). This experiment represents three independent biological replications. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test. (C) Transfected cells were visualized using high-content imaging. Fluorescent signals: green for anti-NRP1 mAb, blue for Hoechst. (D) Percentage of NRP1-expressing cells normalized to cells transfected with siControl. Data are presented as mean ± SEM from three independent experiments. Statistical analysis was conducted using a paired t-test. Next, siRNA-transfected cells were infected with different variants of SARS-CoV-2. (E) SARS-CoV-2-infected cells were detected using high-content imaging. Fluorescent signals: green for anti-SARS-CoV NP mAb, blue for Hoechst. (F) The fluorescent intensity of NP+ cells was measured. All data represent three independent experiments. Statistical analysis among each group of variant was performed using an unpaired t-test. * P < 0.05, ** P < 0.01, *** P = 0.0001, **** P < 0.0001.

    Journal: PLOS One

    Article Title: Omicron SARS-CoV-2 shows NRP1-linked susceptibility in adipose-derived MSCs and alters primitive cord blood CD34⁺ HSPCs

    doi: 10.1371/journal.pone.0360439

    Figure Lengend Snippet: (A) Representative western blot depicting the expression of NRP1 in AD-MSCs (No siRNA), cells transfected with non-targeting siRNA (siControl), or siNRP1 for 24 hours. β-actin was used as a loading control. (B) Graph displaying the quantification of NRP1 normalized to β-actin (n = 3). This experiment represents three independent biological replications. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test. (C) Transfected cells were visualized using high-content imaging. Fluorescent signals: green for anti-NRP1 mAb, blue for Hoechst. (D) Percentage of NRP1-expressing cells normalized to cells transfected with siControl. Data are presented as mean ± SEM from three independent experiments. Statistical analysis was conducted using a paired t-test. Next, siRNA-transfected cells were infected with different variants of SARS-CoV-2. (E) SARS-CoV-2-infected cells were detected using high-content imaging. Fluorescent signals: green for anti-SARS-CoV NP mAb, blue for Hoechst. (F) The fluorescent intensity of NP+ cells was measured. All data represent three independent experiments. Statistical analysis among each group of variant was performed using an unpaired t-test. * P < 0.05, ** P < 0.01, *** P = 0.0001, **** P < 0.0001.

    Article Snippet: The Operetta high-content imaging system (PerkinElmer) was used to detect and analyze the fluorescent signals.

    Techniques: Western Blot, Expressing, Transfection, Control, Analysis, Imaging, Infection, Variant Assay