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halo ai image analysis software v 3 5 (Indica Labs)

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Indica Labs halo ai image analysis software v 3 5
Halo Ai Image Analysis Software V 3 5, supplied by Indica Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+analysis+software/analysis+halo+image+software/pm42315253-299-7-13
Average 86 stars, based on 1 article reviews

halo ai image analysis software v 3 5 - by Bioz Stars, 2026-10

86/100 stars

Images

Multiplex Assay:

Article Title: Virus-specific resident memory T cell networks sustain immunity in human oral mucosa.
Article Snippet: Background: Durable protection against viral infections relies on tissue-resident memory T (TRM) cells at pathogen entry sites.. The oral cavity is a critical mucosal barrier frequently exposed to viral pathogens; however, the phenotypic characteristics and spatial organization of virus-specific T cells in the human oral mucosa remain poorly understood.. Objective: This study aimed to define how virus-specific T cells maintain long-term residency in the oral mucosa and to identify cellular interactions supporting their persistence.

Immunofluorescence:

Software:

Article Title: Integrin α6-targeted photodynamic therapy potentiates PD-1 blockade in MSS colorectal cancer.
Article Snippet: .. H-scores were quantified by HALO® quantitative image analysis software (Indica Labs, USA). .. Human and mouse colorectal adenocarcinoma cell lines (HCT116, SW480, CT26, MC38) and human normal colon epithelial cell lines (FHC, NCM460) were purchased from the American Type Culture Collection (ATCC; USA).

Article Title: POSTN + cancer-associated fibroblasts forming fortress-like barrier to mediate immunotherapy resistance in colorectal cancer.
Article Snippet: 35 Immune checkpoint blockade (ICB) therapy has demonstrated promising efficacy in 36 patients with mismatch repair-deficient (dMMR) or microsatellite instability-high 37 (MSI-H) colorectal cancer (CRC).. However, a substantial proportion of these patients 38 remain resistant to treatment.. This study aims to investigate the mechanisms underlying 39 ICB resistance in dMMR/MSI-H CRCs.

Article Title: Spatial cellular order underlies locally-confined mechanisms of immune resistance in oropharyngeal cancer.
Article Snippet: AR TI CL E IN P RE SS PD-1 Abcam [EPR4877(2)] ab137132 750 Leica ER2(AR9640) / 20 min Akoya OPAL POLYMER HRP MS + RB, 1X, (ARH1001EA) 570 150 CD8 Abcam [EPR10640- 2] ab215041 2000 Leica ER2(AR9640) / 20 min Akoya OPAL POLYMER HRP MS + RB, 1X, (ARH1001EA) 480 150 PanCK Santa Cruz [AE1/AE3] sc-81714 400 Leica ER2(AR9640) / 20 min Akoya OPAL POLYMER HRP MS + RB, 1X, (ARH1001EA) 780 50 .. Whole slide analysis of each stained slide was performed with HALO Image Analysis software (v3.3, Indica Labs). ..

Article Title: ZEB1 integrates stemness, differentiation blockade and immune suppression to orchestrate osteosarcoma aggressiveness.
Article Snippet: Masson's Trichrome staining were performed on murine samples with Diapath kit following the manufacturer’s instructions. .. Masson’s trichrome images were analyzed using the "Area Quantification v2.4.2" algorithm of HALO software (Indica Labs) to quantify the blue-stained collagen fibers. ..

Article Title: Melanoma cell states shape spatial tumor-immune ecosystems to dictate the efficacy of anti-PD1 immunotherapy.
Article Snippet: Segmentation of the scans was performed with inForm version 2.4.8 (Akoya Biosciences). .. The scans were then analyzed using the HALO- AI image analysis software V.3.5 (Indica Labs, London, UK). ..

Staining:

Derivative Assay:

Article Title: Single-Cell Reveal GALNT7-Dependent Ferroptosis Suppression as a Mechanism of Immunotherapy Resistance in Non-Small Cell Lung Cancer.
Article Snippet: Whole-slide luorescence images were acquired on a Zeiss Axio Scan.Z1 sing a Plan-Apochromat 20 ×/0.8 NA objective (whole slide) nd 40 ×/0.95 NA for high-magnification Merge composites and nlarge insets; exposure, gain, and illumination were fixed across amples. .. Quantification was performed in HALO (Indica Labs) 3.x with the HighPlex FL module, using thresholds derived from egative/isotype and single-stain controls and then batch-applied o the cohort. ..

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$Physical attributes of naturally aged mice were enhanced following mGFD‐CD3 treatment. (A) Maximal walking speed, treadmill endurance, hanging endurance, and grip strength of naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded at 6 months after the first infusion ( n = 5 mice per condition). (B) H&E staining of gastrocnemius collected from mice treated with mCD3 or mGFD‐CD3, sampled 6 months after the first infusion. Scale bar, 50 μm. Relative cell areas in the gastrocnemius were quantified using Image J ( n = 5 mice per condition). (C) Representative micro‐CT images of the distal femurs of the mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion. The relative bone volume fraction (BV/TV) of cancellous bone in the distal femur was quantified for each sample and analyzed by Imaris image analysis software ( n = 5 mice per condition). (D) The percentages of total lean mass and total adipose mass and the adipose mass/lean mass ratio of naturally aged mice treated with mCD3 or mGFD‐CD3 6 months after the first infusion were recorded using InAlyzer software ( n = 5 mice per condition). (E) (left) Micro‐CT images of SAT and bone of the naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded 6 months after the first infusion. Green, SAT; red, bone. (right) Quantification of the adipose tissue of mice based on the micro‐CT images ( left ). Data were normalized to mouse body weight ( n = 5 mice per condition). (F) Basal glucose levels after 12 h of starvation in mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion ( n = 5 mice per condition). (G) ( upper ) Levels of glucose before (0 min) and after the intraperitoneal administration of glucose (2 g per kg body weight) (samples as per in (f)). ( lower ) Area under the curve (AUC) for the results from (g) ( n = 5 mice per condition). The mouse experiments are representative results from two independent experiments. Data are presented as mean ± SD and analyzed by one‐way ANOVA followed by Tukey's multiple comparisons test (A–G). * p ≤ 0.05; ** p ≤ 0.01.$

Journal: Aging Cell

Article Title: uPAR ‐Targeting T Cell Engager Exerts Senolytic Effects in Mice and Non‐Human Primates With Serum Aminotransferase Activity as a Safety Monitor

doi: 10.1111/acel.70742

Figure Lengend Snippet: Physical attributes of naturally aged mice were enhanced following mGFD‐CD3 treatment. (A) Maximal walking speed, treadmill endurance, hanging endurance, and grip strength of naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded at 6 months after the first infusion ( n = 5 mice per condition). (B) H&E staining of gastrocnemius collected from mice treated with mCD3 or mGFD‐CD3, sampled 6 months after the first infusion. Scale bar, 50 μm. Relative cell areas in the gastrocnemius were quantified using Image J ( n = 5 mice per condition). (C) Representative micro‐CT images of the distal femurs of the mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion. The relative bone volume fraction (BV/TV) of cancellous bone in the distal femur was quantified for each sample and analyzed by Imaris image analysis software ( n = 5 mice per condition). (D) The percentages of total lean mass and total adipose mass and the adipose mass/lean mass ratio of naturally aged mice treated with mCD3 or mGFD‐CD3 6 months after the first infusion were recorded using InAlyzer software ( n = 5 mice per condition). (E) (left) Micro‐CT images of SAT and bone of the naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded 6 months after the first infusion. Green, SAT; red, bone. (right) Quantification of the adipose tissue of mice based on the micro‐CT images ( left ). Data were normalized to mouse body weight ( n = 5 mice per condition). (F) Basal glucose levels after 12 h of starvation in mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion ( n = 5 mice per condition). (G) ( upper ) Levels of glucose before (0 min) and after the intraperitoneal administration of glucose (2 g per kg body weight) (samples as per in (f)). ( lower ) Area under the curve (AUC) for the results from (g) ( n = 5 mice per condition). The mouse experiments are representative results from two independent experiments. Data are presented as mean ± SD and analyzed by one‐way ANOVA followed by Tukey's multiple comparisons test (A–G). * p ≤ 0.05; ** p ≤ 0.01.

Article Snippet: 3D image analysis was performed with Imaris image analysis software (version 10.1.0).

Techniques: Staining, Micro-CT, Analysis, Software, Tissue