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Structured Review

Proteintech il21
Il21, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il21/IL-21+Antibody/pm41896526-102-29-33
Average 93 stars, based on 7 article reviews
il21 - by Bioz Stars, 2026-10
93/100 stars

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Related Articles

Incubation:

Article Title: The causative effect of CXCR7 on experimental autoimmune prostatitis injury and fibrosis.
Article Snippet: .. The sections are incubated overnight with diluted specific primary antibodies at 4 ◦C (IL-21, 17625–1-AP, Proteintech; α-SMA, AF1032, Affinity). .. Subsequently, an appropriate secondary antibody (IL-17A, ab79056, Abcam) is applied and incubated for 2 h at room temperature in the dark.

Article Title: ZNRF1 deficiency disrupts Fas ligand trafficking and immune balance.
Article Snippet: .. The sections were blocked with 0.3% BSA in 0.1% Triton X-100 at room temperature for 1 h and incubated overnight at 4°C with primary antibodies: Ki67 (1:300, ARG53222, Arigo), IL21 (1:100, 17625- 1, Proteintech), CD3 (1:200, GB12014, Servicebio), and B220 (1:50, 103228, Biolegend). ..

Staining:

Article Title: IL‐21 promoting angiogenesis contributes to the development of psoriasis
Article Snippet: 1Department of Dermatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China 2Center for Translational Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China 3Department of Clinical Laboratory, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China 4Institute of Molecular and Translational Medicine, and Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, China 5Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education, Xi'an, China

Injection:

Article Title: Dual neutralization of TGF-β and IL-21 regulates Th17/Treg balance by suppressing inflammatory signalling in the splenic lymphocytes of Staphylococcus aureus infection-induced septic arthritic mice.
Article Snippet: Septic arthritis (SA) caused by Staphylococcus aureus is a severe inflammatory joint disease, characterized by synovitis accompanied with cartilage destruction and bone erosion.. The available antibiotic treatment alone is insufficient to resolve the inflammation that leads to high rates of morbidity and mortality.. Among the CD4+ T helper lymphocytes, the Th17 and Tregs are key regulators of immune homeostasis.

Infection:

Article Title: Dual neutralization of TGF-β and IL-21 regulates Th17/Treg balance by suppressing inflammatory signalling in the splenic lymphocytes of Staphylococcus aureus infection-induced septic arthritic mice.
Article Snippet: Septic arthritis (SA) caused by Staphylococcus aureus is a severe inflammatory joint disease, characterized by synovitis accompanied with cartilage destruction and bone erosion.. The available antibiotic treatment alone is insufficient to resolve the inflammation that leads to high rates of morbidity and mortality.. Among the CD4+ T helper lymphocytes, the Th17 and Tregs are key regulators of immune homeostasis.



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Expression of genes SOCS1, PTEN , and PIAS3 in PBMC between SLE patients and healthy controls. (A) SOCS1 mRNA expression between HC and SLE patients non-stimulated, (B) SOCS1 mRNA expression between HC and SLE patients after stimulation with PMA+IONO, (C) PTEN mRNA expression between HC and SLE patients non-stimulated, (D) PTEN mRNA expression between HC and SLE patients after stimulation with PMA+IONO, (E) PIAS3 mRNA expression between HC and SLE patients non-stimulated, (F) PIAS3 mRNA expression between HC and SLE patients after stimulation with PMA+IONO, (G) <t>IL21</t> mRNA expression between HC and SLE patients non-stimulated, (H) IL21 mRNA expression between HC and SLE patients after stimulation with PMA+IONO. Data are shown in median and IQR. p-value was obtained using Mann–Whitney’s U test. Fold change values (2 -ΔΔCT ) are relative to healthy controls in mRNA expression. GAPDH served as internal reference normalized for SOCS1 mRNA, PTEN mRNA, PIAS3 mRNA and IL21 mRNA.
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Expression of genes SOCS1, PTEN , and PIAS3 in PBMC between SLE patients and healthy controls. (A) SOCS1 mRNA expression between HC and SLE patients non-stimulated, (B) SOCS1 mRNA expression between HC and SLE patients after stimulation with PMA+IONO, (C) PTEN mRNA expression between HC and SLE patients non-stimulated, (D) PTEN mRNA expression between HC and SLE patients after stimulation with PMA+IONO, (E) PIAS3 mRNA expression between HC and SLE patients non-stimulated, (F) PIAS3 mRNA expression between HC and SLE patients after stimulation with PMA+IONO, (G) <t>IL21</t> mRNA expression between HC and SLE patients non-stimulated, (H) IL21 mRNA expression between HC and SLE patients after stimulation with PMA+IONO. Data are shown in median and IQR. p-value was obtained using Mann–Whitney’s U test. Fold change values (2 -ΔΔCT ) are relative to healthy controls in mRNA expression. GAPDH served as internal reference normalized for SOCS1 mRNA, PTEN mRNA, PIAS3 mRNA and IL21 mRNA.
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Image Search Results


Expression of genes SOCS1, PTEN , and PIAS3 in PBMC between SLE patients and healthy controls. (A) SOCS1 mRNA expression between HC and SLE patients non-stimulated, (B) SOCS1 mRNA expression between HC and SLE patients after stimulation with PMA+IONO, (C) PTEN mRNA expression between HC and SLE patients non-stimulated, (D) PTEN mRNA expression between HC and SLE patients after stimulation with PMA+IONO, (E) PIAS3 mRNA expression between HC and SLE patients non-stimulated, (F) PIAS3 mRNA expression between HC and SLE patients after stimulation with PMA+IONO, (G) IL21 mRNA expression between HC and SLE patients non-stimulated, (H) IL21 mRNA expression between HC and SLE patients after stimulation with PMA+IONO. Data are shown in median and IQR. p-value was obtained using Mann–Whitney’s U test. Fold change values (2 -ΔΔCT ) are relative to healthy controls in mRNA expression. GAPDH served as internal reference normalized for SOCS1 mRNA, PTEN mRNA, PIAS3 mRNA and IL21 mRNA.

Journal: Frontiers in Immunology

Article Title: Aberrant STAT3 activation and overproduction of IL-21 in systemic lupus erythematosus: role of miR-155 and miR-21 in target genes SOCS1, PTEN and PIAS3

doi: 10.3389/fimmu.2026.1664409

Figure Lengend Snippet: Expression of genes SOCS1, PTEN , and PIAS3 in PBMC between SLE patients and healthy controls. (A) SOCS1 mRNA expression between HC and SLE patients non-stimulated, (B) SOCS1 mRNA expression between HC and SLE patients after stimulation with PMA+IONO, (C) PTEN mRNA expression between HC and SLE patients non-stimulated, (D) PTEN mRNA expression between HC and SLE patients after stimulation with PMA+IONO, (E) PIAS3 mRNA expression between HC and SLE patients non-stimulated, (F) PIAS3 mRNA expression between HC and SLE patients after stimulation with PMA+IONO, (G) IL21 mRNA expression between HC and SLE patients non-stimulated, (H) IL21 mRNA expression between HC and SLE patients after stimulation with PMA+IONO. Data are shown in median and IQR. p-value was obtained using Mann–Whitney’s U test. Fold change values (2 -ΔΔCT ) are relative to healthy controls in mRNA expression. GAPDH served as internal reference normalized for SOCS1 mRNA, PTEN mRNA, PIAS3 mRNA and IL21 mRNA.

Article Snippet: TaqMan probes (Applied Biosystems, Foster City, CA, USA) were used for qPCR: SOCS1 (Hs00705164_s1), PTEN (Hs0262621230_s1), PIAS3 (Hs00966035_m1) and IL21 (Hs00222327_m1), using the following incubation conditions: 50°C for 2 minutes, 95°C for 20 seconds and 40 cycles of 95°C for 1 second and 60°C for 20 seconds.

Techniques: Expressing

Protein expression of IL-21, p-STAT3, SOCS1, PTEN and PIAS3. (A) IL-21 expression in HC and SLE patients non-stimulated, (B) p-STAT3 expression in HC and SLE patients non-stimulated, (C) SOCS1 expression in HC and SLE patients non-stimulated, (D) PTEN expression in HC and SLE patients non-stimulated, (E) PIAS3 expression in HC and SLE patients non-stimulated, (F) IL-21 expression after stimulation with PMA+IONO in HC and SLE patients, (G) p-STAT3 expression after stimulation with PMA+IONO in HC and SLE patients, (H) SOCS1 expression after stimulation with PMA+IONO in HC and SLE patients, (I) PTEN expression after stimulation with PMA+IONO in HC and SLE patients, (J) PIAS3 expression after stimulation with PMA+IONO in HC and SLE patients. Data are shown in median and IQR. p-value was obtained through Mann-Whitney’s U test. β-actin served as an internal reference.

Journal: Frontiers in Immunology

Article Title: Aberrant STAT3 activation and overproduction of IL-21 in systemic lupus erythematosus: role of miR-155 and miR-21 in target genes SOCS1, PTEN and PIAS3

doi: 10.3389/fimmu.2026.1664409

Figure Lengend Snippet: Protein expression of IL-21, p-STAT3, SOCS1, PTEN and PIAS3. (A) IL-21 expression in HC and SLE patients non-stimulated, (B) p-STAT3 expression in HC and SLE patients non-stimulated, (C) SOCS1 expression in HC and SLE patients non-stimulated, (D) PTEN expression in HC and SLE patients non-stimulated, (E) PIAS3 expression in HC and SLE patients non-stimulated, (F) IL-21 expression after stimulation with PMA+IONO in HC and SLE patients, (G) p-STAT3 expression after stimulation with PMA+IONO in HC and SLE patients, (H) SOCS1 expression after stimulation with PMA+IONO in HC and SLE patients, (I) PTEN expression after stimulation with PMA+IONO in HC and SLE patients, (J) PIAS3 expression after stimulation with PMA+IONO in HC and SLE patients. Data are shown in median and IQR. p-value was obtained through Mann-Whitney’s U test. β-actin served as an internal reference.

Article Snippet: TaqMan probes (Applied Biosystems, Foster City, CA, USA) were used for qPCR: SOCS1 (Hs00705164_s1), PTEN (Hs0262621230_s1), PIAS3 (Hs00966035_m1) and IL21 (Hs00222327_m1), using the following incubation conditions: 50°C for 2 minutes, 95°C for 20 seconds and 40 cycles of 95°C for 1 second and 60°C for 20 seconds.

Techniques: Expressing

Cytokines IL-21 and IL-17A levels. (A) IL-21 plasma levels in HC and SLE patients, (B) comparison of IL-21 levels between disease activity groups according to Mex-SLEDAI, (C) IL-21 cytokine measurement in the cell culture supernatant of PMA+IONO-stimulated PBMC, (D) IL-17A levels measurement in the cell culture supernatant of PMA+IONO-stimulated PBMC. Data are shown in median and IQR. p-value was obtained through Mann-Whitney’s U test and Kruskal-Wallis with Dunn’s post-hoc , according to the case.

Journal: Frontiers in Immunology

Article Title: Aberrant STAT3 activation and overproduction of IL-21 in systemic lupus erythematosus: role of miR-155 and miR-21 in target genes SOCS1, PTEN and PIAS3

doi: 10.3389/fimmu.2026.1664409

Figure Lengend Snippet: Cytokines IL-21 and IL-17A levels. (A) IL-21 plasma levels in HC and SLE patients, (B) comparison of IL-21 levels between disease activity groups according to Mex-SLEDAI, (C) IL-21 cytokine measurement in the cell culture supernatant of PMA+IONO-stimulated PBMC, (D) IL-17A levels measurement in the cell culture supernatant of PMA+IONO-stimulated PBMC. Data are shown in median and IQR. p-value was obtained through Mann-Whitney’s U test and Kruskal-Wallis with Dunn’s post-hoc , according to the case.

Article Snippet: TaqMan probes (Applied Biosystems, Foster City, CA, USA) were used for qPCR: SOCS1 (Hs00705164_s1), PTEN (Hs0262621230_s1), PIAS3 (Hs00966035_m1) and IL21 (Hs00222327_m1), using the following incubation conditions: 50°C for 2 minutes, 95°C for 20 seconds and 40 cycles of 95°C for 1 second and 60°C for 20 seconds.

Techniques: Clinical Proteomics, Comparison, Activity Assay, Cell Culture

Correlation among miR-155, miR-21, SOCS1 mRNA, PTEN mRNA, PIAS3 mRNA and IL21 mRNA in HC and SLE patients. (A) correlation between the miR-155, miR-21, SOCS1 mRNA, PTEN mRNA, PIAS3 mRNA and IL21 mRNA non-stimulated in HC, (B) correlation between the miR-155, miR-21, SOCS1 mRNA, PTEN mRNA, PIAS3 mRNA and IL21 mRNA after stimulation with PMA+IONO in HC, (C) correlation between the miR-155, miR-21, SOCS1 mRNA, PTEN mRNA, PIAS3 mRNA and IL21 mRNA non-stimulated in SLE patients, (D) correlation between the miR-155, miR-21, SOCS1 mRNA, PTEN mRNA, PIAS3 mRNA and IL21 mRNA after stimulation with PMA+IONO in SLE patients. p-value was obtained using Spearman’s correlation. *p ≤ 0.05, **p ≤ 0.001, ***p ≤ 0.0001.

Journal: Frontiers in Immunology

Article Title: Aberrant STAT3 activation and overproduction of IL-21 in systemic lupus erythematosus: role of miR-155 and miR-21 in target genes SOCS1, PTEN and PIAS3

doi: 10.3389/fimmu.2026.1664409

Figure Lengend Snippet: Correlation among miR-155, miR-21, SOCS1 mRNA, PTEN mRNA, PIAS3 mRNA and IL21 mRNA in HC and SLE patients. (A) correlation between the miR-155, miR-21, SOCS1 mRNA, PTEN mRNA, PIAS3 mRNA and IL21 mRNA non-stimulated in HC, (B) correlation between the miR-155, miR-21, SOCS1 mRNA, PTEN mRNA, PIAS3 mRNA and IL21 mRNA after stimulation with PMA+IONO in HC, (C) correlation between the miR-155, miR-21, SOCS1 mRNA, PTEN mRNA, PIAS3 mRNA and IL21 mRNA non-stimulated in SLE patients, (D) correlation between the miR-155, miR-21, SOCS1 mRNA, PTEN mRNA, PIAS3 mRNA and IL21 mRNA after stimulation with PMA+IONO in SLE patients. p-value was obtained using Spearman’s correlation. *p ≤ 0.05, **p ≤ 0.001, ***p ≤ 0.0001.

Article Snippet: TaqMan probes (Applied Biosystems, Foster City, CA, USA) were used for qPCR: SOCS1 (Hs00705164_s1), PTEN (Hs0262621230_s1), PIAS3 (Hs00966035_m1) and IL21 (Hs00222327_m1), using the following incubation conditions: 50°C for 2 minutes, 95°C for 20 seconds and 40 cycles of 95°C for 1 second and 60°C for 20 seconds.

Techniques: