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sarcomas  (Novus Biologicals)


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    Structured Review

    Novus Biologicals sarcomas
    Sarcomas, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+tissue+microarray/Human+Various+Tissue+MicroArray+(Sarcoma)/pmc08319167-181-9-16
    Average 90 stars, based on 3 article reviews
    sarcomas - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Immunohistochemistry:

    Article Title: PIK3R3, part of the regulatory domain of PI3K, is upregulated in sarcoma stem-like cells and promotes invasion, migration, and chemotherapy resistance
    Article Snippet: Commercially available paraffin-embedded tissue array slides containing 79 human sarcomas and 4 human normal tissues (NBP2-30332, Novus Biologicals, and T242, US Biomax, Inc., USA) were purchased.. Sections were deparaffinized, then incubated with anti-human CD133, PIK3R3 (SR44-06; Novus Biologicals), and Nanog (ab80892; Abcam) in a solution of PBS with 1% BSA and 0.1% Triton X-100 at 4 °C overnight.Sections were deparaffinized, then incubated with anti-human CD133, PIK3R3 (SR44-06; Novus Biologicals), and Nanog (ab80892; Abcam) in a solution of PBS with 1% BSA and 0.1% Triton X-100 at 4 °C overnight.

    Article Title: PIK3R3, part of the regulatory domain of PI3K, is upregulated in sarcoma stem-like cells and promotes invasion, migration, and chemotherapy resistance
    Article Snippet: Protein expression of PIK3R3 and the stem cell transcription factor Nanog were greater in CD133+ compared with CD133– cells (Fig. ), while levels of other stem cell transcription factors including Sox2, Oct-4, and c-Myc were not consistently increased.Protein expression of PIK3R3 and the stem cell transcription factor Nanog were greater in CD133+ compared with CD133– cells (Fig. ), while levels of other stem cell transcription factors including Sox2, Oct-4, and c-Myc were not consistently increased.. Finally, to examine whether PIK3R3 is generally overexpressed in human sarcomas, we immunostained commercially available tissue microarrays containing 79 human sarcomas (including diverse subtypes) and 4 human normal tissues (NBP2-30332, Novus Biologicals, and T242, US Biomax).. Positive cells of CD133, Nanog, or PIK3R3 were 5.2–12.7-fold higher in tumor tissue compared with normal tissue as determined by immunostaining (Fig. ).Positive cells of CD133, Nanog, or PIK3R3 were 5.2–12.7-fold higher in tumor tissue compared with normal tissue as determined by immunostaining (Fig. ).

    Article Title: PIK3R3, part of the regulatory domain of PI3K, is upregulated in sarcoma stem-like cells and promotes invasion, migration, and chemotherapy resistance
    Article Snippet: examine whether PIK3R3 is generally overexpressed in human sarcomas, we immunostained commercially available tissue microarrays containing 79 human sarcomas (including diverse subtypes) and 4 human normal tissues (NBP2-30332, Novus Biologicals, and T242, US Biomax). Positive cells of CD133, Nanog, or PIK3R3 were 5.2–12.7-fold higher in tumor tissue compared with normal tissue as determined by immunostaining (Fig. 1F ). Together, these results show

    Article Title: Enrichment of the embryonic stem cell reprogramming factors Oct4, Nanog, Myc, and Sox2 in benign and malignant vascular tumors
    Article Snippet: Blood vessel disease spectrum tissue arrays containing various vascular tumors and non-diseased controls were purchased from US Biomax, Inc. (#SO8010).Blood vessel disease spectrum tissue arrays containing various vascular tumors and non-diseased controls were purchased from US Biomax, Inc. (#SO8010).. The sarcoma tissue arrays were purchased from Novus Biologicals (#NBP2 = 30332).. For detection of protein expression, tissue arrays were labeled with anti-Myc (Cat# ab32072; Abcam), anti-Oct4 (Cat# ab18976; Abcam), anti-Sox2 (Cat# ab97959; Abcam), anti-Klf4 (Cat# ab118961; Abcam), and anti-Nanog (Cat# ab80892; Abcam) antibodies.For detection of protein expression, tissue arrays were labeled with anti-Myc (Cat# ab32072; Abcam), anti-Oct4 (Cat# ab18976; Abcam), anti-Sox2 (Cat# ab97959; Abcam), anti-Klf4 (Cat# ab118961; Abcam), and anti-Nanog (Cat# ab80892; Abcam) antibodies.

    Immunofluorescence:

    Article Title: PIK3R3, part of the regulatory domain of PI3K, is upregulated in sarcoma stem-like cells and promotes invasion, migration, and chemotherapy resistance
    Article Snippet: Commercially available paraffin-embedded tissue array slides containing 79 human sarcomas and 4 human normal tissues (NBP2-30332, Novus Biologicals, and T242, US Biomax, Inc., USA) were purchased.. Sections were deparaffinized, then incubated with anti-human CD133, PIK3R3 (SR44-06; Novus Biologicals), and Nanog (ab80892; Abcam) in a solution of PBS with 1% BSA and 0.1% Triton X-100 at 4 °C overnight.Sections were deparaffinized, then incubated with anti-human CD133, PIK3R3 (SR44-06; Novus Biologicals), and Nanog (ab80892; Abcam) in a solution of PBS with 1% BSA and 0.1% Triton X-100 at 4 °C overnight.

    Article Title: PIK3R3, part of the regulatory domain of PI3K, is upregulated in sarcoma stem-like cells and promotes invasion, migration, and chemotherapy resistance
    Article Snippet: Protein expression of PIK3R3 and the stem cell transcription factor Nanog were greater in CD133+ compared with CD133– cells (Fig. ), while levels of other stem cell transcription factors including Sox2, Oct-4, and c-Myc were not consistently increased.Protein expression of PIK3R3 and the stem cell transcription factor Nanog were greater in CD133+ compared with CD133– cells (Fig. ), while levels of other stem cell transcription factors including Sox2, Oct-4, and c-Myc were not consistently increased.. Finally, to examine whether PIK3R3 is generally overexpressed in human sarcomas, we immunostained commercially available tissue microarrays containing 79 human sarcomas (including diverse subtypes) and 4 human normal tissues (NBP2-30332, Novus Biologicals, and T242, US Biomax).. Positive cells of CD133, Nanog, or PIK3R3 were 5.2–12.7-fold higher in tumor tissue compared with normal tissue as determined by immunostaining (Fig. ).Positive cells of CD133, Nanog, or PIK3R3 were 5.2–12.7-fold higher in tumor tissue compared with normal tissue as determined by immunostaining (Fig. ).

    Article Title: PIK3R3, part of the regulatory domain of PI3K, is upregulated in sarcoma stem-like cells and promotes invasion, migration, and chemotherapy resistance
    Article Snippet: examine whether PIK3R3 is generally overexpressed in human sarcomas, we immunostained commercially available tissue microarrays containing 79 human sarcomas (including diverse subtypes) and 4 human normal tissues (NBP2-30332, Novus Biologicals, and T242, US Biomax). Positive cells of CD133, Nanog, or PIK3R3 were 5.2–12.7-fold higher in tumor tissue compared with normal tissue as determined by immunostaining (Fig. 1F ). Together, these results show

    Article Title: Enrichment of the embryonic stem cell reprogramming factors Oct4, Nanog, Myc, and Sox2 in benign and malignant vascular tumors
    Article Snippet: Blood vessel disease spectrum tissue arrays containing various vascular tumors and non-diseased controls were purchased from US Biomax, Inc. (#SO8010).Blood vessel disease spectrum tissue arrays containing various vascular tumors and non-diseased controls were purchased from US Biomax, Inc. (#SO8010).. The sarcoma tissue arrays were purchased from Novus Biologicals (#NBP2 = 30332).. For detection of protein expression, tissue arrays were labeled with anti-Myc (Cat# ab32072; Abcam), anti-Oct4 (Cat# ab18976; Abcam), anti-Sox2 (Cat# ab97959; Abcam), anti-Klf4 (Cat# ab118961; Abcam), and anti-Nanog (Cat# ab80892; Abcam) antibodies.For detection of protein expression, tissue arrays were labeled with anti-Myc (Cat# ab32072; Abcam), anti-Oct4 (Cat# ab18976; Abcam), anti-Sox2 (Cat# ab97959; Abcam), anti-Klf4 (Cat# ab118961; Abcam), and anti-Nanog (Cat# ab80892; Abcam) antibodies.



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    Image Search Results


    Overexpression of DNPH1 in human breast tumors (A) DNPH1 mRNA expression in various breast cancer subtypes was analyzed in published microarray data. Mean with standard deviation and statistical significance compared to normal breast tissue are indicated; ∗∗∗∗, p < 0.0001 (one-way ANOVA, Tukey’s multiple comparisons test). (B) High (top quartile) DNPH1 mRNA levels predict reduced survival. Data derived from published microarray experiments ; p < 0.0001 (log rank test). (C) DNPH1 protein expression in human breast cell lines assayed by Western blotting. (D) Examples of immunohistochemical staining with DNPH1 antibodies on matching normal and cancerous breast tissue. Scale bars, 0.1 mm. (E) DNPH1 protein expression was measured by immunohistochemistry in 20 matching normal and cancerous human breast tissues; two-tailed, paired t test.

    Journal: iScience

    Article Title: Promotion of breast cancer by the DNPH1 enzyme

    doi: 10.1016/j.isci.2026.115227

    Figure Lengend Snippet: Overexpression of DNPH1 in human breast tumors (A) DNPH1 mRNA expression in various breast cancer subtypes was analyzed in published microarray data. Mean with standard deviation and statistical significance compared to normal breast tissue are indicated; ∗∗∗∗, p < 0.0001 (one-way ANOVA, Tukey’s multiple comparisons test). (B) High (top quartile) DNPH1 mRNA levels predict reduced survival. Data derived from published microarray experiments ; p < 0.0001 (log rank test). (C) DNPH1 protein expression in human breast cell lines assayed by Western blotting. (D) Examples of immunohistochemical staining with DNPH1 antibodies on matching normal and cancerous breast tissue. Scale bars, 0.1 mm. (E) DNPH1 protein expression was measured by immunohistochemistry in 20 matching normal and cancerous human breast tissues; two-tailed, paired t test.

    Article Snippet: Human breast tissue microarray , ISU Abxis , A312.

    Techniques: Over Expression, Expressing, Microarray, Standard Deviation, Derivative Assay, Western Blot, Immunohistochemical staining, Staining, Immunohistochemistry, Two Tailed Test

    Myxoid liposarcoma tissues present low levels of CD99 expression. A Representative image of tissue microarray staining of CD99 in Ewing’s sarcoma, liposarcoma myxoid, osteosarcoma, and chondrosarcoma taken with Objective 10 ×. B Quantification of CD99 signal in cancer tissue microarray. Each dot represents one patient-derived sample, where the signal was normalized to Ewing’s sarcoma signal, and then regrouped by cancer type. N = 2 independent experiments were averaged. Ewing’s sarcoma is represented by a dotted line at 100% and serves as a positive control for CD99 abundance level as previously reported

    Journal: BMC Biology

    Article Title: Proteomics analysis of human mesenchymal stromal/stem cell sarcomagenesis model identifies ALDH1A3 and CD99 as potential targets in the transformation process

    doi: 10.1186/s12915-025-02498-z

    Figure Lengend Snippet: Myxoid liposarcoma tissues present low levels of CD99 expression. A Representative image of tissue microarray staining of CD99 in Ewing’s sarcoma, liposarcoma myxoid, osteosarcoma, and chondrosarcoma taken with Objective 10 ×. B Quantification of CD99 signal in cancer tissue microarray. Each dot represents one patient-derived sample, where the signal was normalized to Ewing’s sarcoma signal, and then regrouped by cancer type. N = 2 independent experiments were averaged. Ewing’s sarcoma is represented by a dotted line at 100% and serves as a positive control for CD99 abundance level as previously reported

    Article Snippet: The tissue microarray (#NBP2-30,330) was purchased from Novus Biologicals (Toronto, ON, Canada).

    Techniques: Expressing, Microarray, Staining, Derivative Assay, Positive Control