nbp2  (Novus Biologicals)

Journal: iScience

Article Title: Promotion of breast cancer by the DNPH1 enzyme

doi: 10.1016/j.isci.2026.115227

Figure Lengend Snippet: Overexpression of DNPH1 in human breast tumors (A) DNPH1 mRNA expression in various breast cancer subtypes was analyzed in published microarray data. Mean with standard deviation and statistical significance compared to normal breast tissue are indicated; ∗∗∗∗, p < 0.0001 (one-way ANOVA, Tukey’s multiple comparisons test). (B) High (top quartile) DNPH1 mRNA levels predict reduced survival. Data derived from published microarray experiments ; p < 0.0001 (log rank test). (C) DNPH1 protein expression in human breast cell lines assayed by Western blotting. (D) Examples of immunohistochemical staining with DNPH1 antibodies on matching normal and cancerous breast tissue. Scale bars, 0.1 mm. (E) DNPH1 protein expression was measured by immunohistochemistry in 20 matching normal and cancerous human breast tissues; two-tailed, paired t test.

Article Snippet: Human breast tissue microarray , ISU Abxis , A312.

Techniques: Over Expression, Expressing, Microarray, Standard Deviation, Derivative Assay, Western Blot, Immunohistochemical staining, Staining, Immunohistochemistry, Two Tailed Test

Journal: BMC Biology

Article Title: Proteomics analysis of human mesenchymal stromal/stem cell sarcomagenesis model identifies ALDH1A3 and CD99 as potential targets in the transformation process

doi: 10.1186/s12915-025-02498-z

Figure Lengend Snippet: Myxoid liposarcoma tissues present low levels of CD99 expression. A Representative image of tissue microarray staining of CD99 in Ewing’s sarcoma, liposarcoma myxoid, osteosarcoma, and chondrosarcoma taken with Objective 10 ×. B Quantification of CD99 signal in cancer tissue microarray. Each dot represents one patient-derived sample, where the signal was normalized to Ewing’s sarcoma signal, and then regrouped by cancer type. N = 2 independent experiments were averaged. Ewing’s sarcoma is represented by a dotted line at 100% and serves as a positive control for CD99 abundance level as previously reported

Article Snippet: The tissue microarray (#NBP2-30,330) was purchased from Novus Biologicals (Toronto, ON, Canada).

Techniques: Expressing, Microarray, Staining, Derivative Assay, Positive Control