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normal human serum standard  (Quidel)


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    Quidel normal human serum standard
    Normal Human Serum Standard, supplied by Quidel, used in various techniques. Bioz Stars score: 95/100, based on 232 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+complement+serum/Normal+Human+Serum+-+Complement/pm41707327-64-0-12
    Average 95 stars, based on 232 article reviews
    normal human serum standard - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: EMB ‐01, a Tetravalent Bispecific Antibody, Inducing Co‐Degradation of EGFR and c‐Met for Enhanced Anti‐Tumor Efficacy
    Article Snippet: Cytotoxicity was measured using the CytoTox‐ONE Homogeneous Membrane Integrity Assay (Promega, Cat G7891), following the manufacturer's instructions. .. HCC827 cells were incubated with serial dilutions of EMB‐01 or control antibody, followed by the addition of normal human serum complement (Quidel Corporation, Cat A113). .. After overnight incubation at 37°C, cell viability was assessed using the CellTiter‐Glo Luminescent Cell Viability Assay (Promega, Cat G7573).

    Article Title: Xenopus IgX informs engineering strategies of IgM and IgG hexamers
    Article Snippet: .. The RTX-Fcμ or RTX-Fcγ proteins were incubated with equal volumes of cell cultures (~20,000 cells) and 12% normal human serum complement (Quidel) sequentially and then transferred into a 96-microwell plate. .. After 8 hours of incubation at 37°C, 50 μl of CellTiter-Glo reagent (Promega, G7572) was added to each well and incubated for 10 min at room temperature.

    Article Title: Xenopus IgX informs engineering strategies of IgM and IgG hexamers.
    Article Snippet: .. The RTX- Fcμ or RTX- Fcγ proteins were incubated with equal volumes of cell cultures (~20,000 cells) and 12% normal human serum complement (Quidel) sequentially and then transferred into a 96- microwell plate. .. After 8 hours of incubation at 37°C, 50 μl of CellTiter- Glo reagent (Promega, G7572) was added to each well and incubated for 10 min at room temperature.

    Article Title: RNA particles comprising polysarcosine
    Article Snippet: Complement Activation In vitro C3a levels were determined using Human C3a EIA kit (Quidel). .. Briefly, LNPs and controls (positive (Cremophore El) and negative (1×PBS and EDTA (18 mM)) were incubated with Normal Human Serum Complement (NHS, Quidel) at a ratio of 20:80 (Specimen:NHS) for 1 h at 37° C. LNPs were tested at 5×, 1× and 0.02× based on a theoretical plasma concentration of 1 mg/kg mRNA dose. ..

    Article Title: EMB-01, a Tetravalent Bispecific Antibody, Inducing Co-Degradation of EGFR and c-Met for Enhanced Anti-Tumor Efficacy.
    Article Snippet: Cytotoxicity was measured using the CytoTox- ONE Homogeneous Membrane Integrity Assay (Promega, Cat G7891), following the manufacturer's instructions. nloaded from https://onlinelibrary.w iley.com /doi/10.1111/cas.70280, W iley O nline L ibrary on [20/12/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 3Cancer Science, 2025 HCC827 cells were incubated with serial dilutions of EMB- 01 or control antibody, followed by the addition of normal human serum complement (Quidel Corporation, Cat A113). .. After overnight incubation at 37°C, cell viability was assessed using the CellTiter- Glo Luminescent Cell Viability Assay (Promega, Cat G7573).

    Control:

    Article Title: EMB ‐01, a Tetravalent Bispecific Antibody, Inducing Co‐Degradation of EGFR and c‐Met for Enhanced Anti‐Tumor Efficacy
    Article Snippet: Cytotoxicity was measured using the CytoTox‐ONE Homogeneous Membrane Integrity Assay (Promega, Cat G7891), following the manufacturer's instructions. .. HCC827 cells were incubated with serial dilutions of EMB‐01 or control antibody, followed by the addition of normal human serum complement (Quidel Corporation, Cat A113). .. After overnight incubation at 37°C, cell viability was assessed using the CellTiter‐Glo Luminescent Cell Viability Assay (Promega, Cat G7573).

    Article Title: EMB-01, a Tetravalent Bispecific Antibody, Inducing Co-Degradation of EGFR and c-Met for Enhanced Anti-Tumor Efficacy.
    Article Snippet: Cytotoxicity was measured using the CytoTox- ONE Homogeneous Membrane Integrity Assay (Promega, Cat G7891), following the manufacturer's instructions. nloaded from https://onlinelibrary.w iley.com /doi/10.1111/cas.70280, W iley O nline L ibrary on [20/12/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 3Cancer Science, 2025 HCC827 cells were incubated with serial dilutions of EMB- 01 or control antibody, followed by the addition of normal human serum complement (Quidel Corporation, Cat A113). .. After overnight incubation at 37°C, cell viability was assessed using the CellTiter- Glo Luminescent Cell Viability Assay (Promega, Cat G7573).

    Clinical Proteomics:

    Article Title: RNA particles comprising polysarcosine
    Article Snippet: Complement Activation In vitro C3a levels were determined using Human C3a EIA kit (Quidel). .. Briefly, LNPs and controls (positive (Cremophore El) and negative (1×PBS and EDTA (18 mM)) were incubated with Normal Human Serum Complement (NHS, Quidel) at a ratio of 20:80 (Specimen:NHS) for 1 h at 37° C. LNPs were tested at 5×, 1× and 0.02× based on a theoretical plasma concentration of 1 mg/kg mRNA dose. ..

    Concentration Assay:

    Article Title: RNA particles comprising polysarcosine
    Article Snippet: Complement Activation In vitro C3a levels were determined using Human C3a EIA kit (Quidel). .. Briefly, LNPs and controls (positive (Cremophore El) and negative (1×PBS and EDTA (18 mM)) were incubated with Normal Human Serum Complement (NHS, Quidel) at a ratio of 20:80 (Specimen:NHS) for 1 h at 37° C. LNPs were tested at 5×, 1× and 0.02× based on a theoretical plasma concentration of 1 mg/kg mRNA dose. ..

    Article Title: Compositions and methods for using bispecific antibodies to bind complement and a target antigen
    Article Snippet: .. Bacteria were subsequently mixed with an equal volume of normal human serum (Quidel) or human serum depleted of a terminal complement protein (Quidel) such that the final concentration of serum was 50%. ..

    Bacteria:

    Article Title: Compositions and methods for using bispecific antibodies to bind complement and a target antigen
    Article Snippet: .. Bacteria were subsequently mixed with an equal volume of normal human serum (Quidel) or human serum depleted of a terminal complement protein (Quidel) such that the final concentration of serum was 50%. ..

    Transfection:

    Article Title: Anti-PD-L1 antibodies
    Article Snippet: TABLE 1 Commercial materials Materials Vendor Cat. PE mouse anti-human CD274 Ab eBioscience Cat. #12-5983-42 PE rat anti-mouse CD274 Ab eBioscience Cat. #12-4317-87 PE goat anti-mouse IgG Fc Abcam Cat. #ab98742 R-PE goat anti-human IgG Fc Jackson Immuno Cat. #109-115-098 Research PE goat anti-mouse IgG Fc Abcam Cat. #Ab98742 Human PD-L1, ECD, mFc tag Sino Biological Cat. #10084-H05H SA-PE eBioscience Cat. #12-4317-87 HRP goat anti-human IgG Fc Bethyl Cat. #A80-304P Ficoll-Paque TM PLUS GE Healthcare Cat. #17-1440-02 CD14 MicroBeads, human Milteny Cat. #130-050-201 Human CD4+ T Cell Enrichment kit STEMCELL Cat. #19052 Recombinant human GM-CSF Amoytop Cat. #S10980039 Biotech Recombinant human IL-4 R&D Cat. #204-IL-010 Standard recombinant human IFN-γ PeproTech Cat. #300-02 Human IFN-γ capture antibody Pierce Cat. #M700A Human IFN-γ detection antibody Pierce Cat. #M701B SA-HRP Invitrogen Cat. #SNN1004 Standard recombinant human IL-2 R&D Cat. #202-IL-010 Human IL-2 capture antibody R&D Cat. #MAB602 Human IL-2 detection antibody R&D Cat. #BAF202 Mouse IL-2 ELISA kit BD Cat. #2614KI CMV pp65 peptide Miltenyi Biotec Cat. #130-093-435 LPS Sigma Cat. #L5418 H3-thymidine PerkinElmer Cat. #NET027001MC MicroScint-20 PerkinElmer Cat. #6013621 Herceptin Roche Lot. .. #N3550 Human Serum Complement Quidel Cat. #A112 Cytotoxicity Detection Kit Roche Cat. #11644793001 CellTiter Glo Kit Promega Cat. #G7571 BT-474 cell line ATCC Cat. # HTB-20 Raji cell line ATCC Cat. #CCL-86 Lipofectamine TM 2000 Transfection Invitrogen Cat. #11668019 Reagent Ham's F-12K (Kaighn's) Medium Gibco Cat. #21127022 FreeStyle TM 293 Expression Medium Gibco Cat. #12338002 Fetal Bovine Serum HyClone Cat. #SV30087.03 Blasticidin Gibco Cat. #R21001 1.2 Material Code The codes or abbreviation for the materials including benchmark antibodies, the extracellular domains and the cells are summarized in Table 2. ..

    Expressing:

    Article Title: Anti-PD-L1 antibodies
    Article Snippet: TABLE 1 Commercial materials Materials Vendor Cat. PE mouse anti-human CD274 Ab eBioscience Cat. #12-5983-42 PE rat anti-mouse CD274 Ab eBioscience Cat. #12-4317-87 PE goat anti-mouse IgG Fc Abcam Cat. #ab98742 R-PE goat anti-human IgG Fc Jackson Immuno Cat. #109-115-098 Research PE goat anti-mouse IgG Fc Abcam Cat. #Ab98742 Human PD-L1, ECD, mFc tag Sino Biological Cat. #10084-H05H SA-PE eBioscience Cat. #12-4317-87 HRP goat anti-human IgG Fc Bethyl Cat. #A80-304P Ficoll-Paque TM PLUS GE Healthcare Cat. #17-1440-02 CD14 MicroBeads, human Milteny Cat. #130-050-201 Human CD4+ T Cell Enrichment kit STEMCELL Cat. #19052 Recombinant human GM-CSF Amoytop Cat. #S10980039 Biotech Recombinant human IL-4 R&D Cat. #204-IL-010 Standard recombinant human IFN-γ PeproTech Cat. #300-02 Human IFN-γ capture antibody Pierce Cat. #M700A Human IFN-γ detection antibody Pierce Cat. #M701B SA-HRP Invitrogen Cat. #SNN1004 Standard recombinant human IL-2 R&D Cat. #202-IL-010 Human IL-2 capture antibody R&D Cat. #MAB602 Human IL-2 detection antibody R&D Cat. #BAF202 Mouse IL-2 ELISA kit BD Cat. #2614KI CMV pp65 peptide Miltenyi Biotec Cat. #130-093-435 LPS Sigma Cat. #L5418 H3-thymidine PerkinElmer Cat. #NET027001MC MicroScint-20 PerkinElmer Cat. #6013621 Herceptin Roche Lot. .. #N3550 Human Serum Complement Quidel Cat. #A112 Cytotoxicity Detection Kit Roche Cat. #11644793001 CellTiter Glo Kit Promega Cat. #G7571 BT-474 cell line ATCC Cat. # HTB-20 Raji cell line ATCC Cat. #CCL-86 Lipofectamine TM 2000 Transfection Invitrogen Cat. #11668019 Reagent Ham's F-12K (Kaighn's) Medium Gibco Cat. #21127022 FreeStyle TM 293 Expression Medium Gibco Cat. #12338002 Fetal Bovine Serum HyClone Cat. #SV30087.03 Blasticidin Gibco Cat. #R21001 1.2 Material Code The codes or abbreviation for the materials including benchmark antibodies, the extracellular domains and the cells are summarized in Table 2. ..



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    Quidel normal human serum complement
    ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human <t>complement.</t> Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.
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    95
    Quidel human serum
    ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human <t>complement.</t> Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.
    Human Serum, supplied by Quidel, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human complement. Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.

    Journal: Science Advances

    Article Title: Xenopus IgX informs engineering strategies of IgM and IgG hexamers

    doi: 10.1126/sciadv.aea3737

    Figure Lengend Snippet: ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human complement. Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.

    Article Snippet: The RTX-Fcμ or RTX-Fcγ proteins were incubated with equal volumes of cell cultures (~20,000 cells) and 12% normal human serum complement (Quidel) sequentially and then transferred into a 96-microwell plate.

    Techniques: Cell Culture