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human serum complement  (Quidel)


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    Quidel human serum complement
    Human Serum Complement, supplied by Quidel, used in various techniques. Bioz Stars score: 96/100, based on 184 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+complement+serum/Human+Complement+Standard/us12497457-1471-0-3
    Average 96 stars, based on 184 article reviews
    human serum complement - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    other:

    Article Title: Methods of treatment or prevention of immune responses and inflammatory diseases with antagonistic CD40 monoclonal antibodies
    Article Snippet: The target cells were Raji cells which endogenously express CD40 (obtained from ATCC).

    Article Title: Antagonistic CD40 monoclonal antibodies and uses thereof
    Article Snippet: Luminescence data was then acquired with a PerkinElmer EnVision® Plate Reader (PerkinElmer, Waltham, MA).

    Incubation:

    Article Title: Compositions and methods related to engineered Fc constructs
    Article Snippet: Female C57BL/6 mice weighing 18-22 grams were purchased from Charles River Labs and used in the studies after one week of acclimation. .. Human serum complement (Quidel) was added to all wells at 25% v/v and incubated for 2 h at 37° C. Cells were incubated for 12 h at 37° C. after addition of WST-1 cell proliferation reagent (Roche Applied Science). ..

    Binding Assay:

    Article Title: Comparative Preclinical Evaluation of Tuznue Versus Referent Herceptin: A Registered Trastuzumab Biosimilar.
    Article Snippet: .. The binding affinity of Tuznue®, US-based Herceptin® (USHerceptin®), or EU-based Herceptin® (EU-Herceptin®) to human C1q complement was determined using CIC-C1q EIA kit (MicroVueTM; QuidelOrtho Corporation, San Diego, CA, USA). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Comparative Preclinical Evaluation of Tuznue Versus Referent Herceptin: A Registered Trastuzumab Biosimilar.
    Article Snippet: .. The binding affinity of Tuznue®, US-based Herceptin® (USHerceptin®), or EU-based Herceptin® (EU-Herceptin®) to human C1q complement was determined using CIC-C1q EIA kit (MicroVueTM; QuidelOrtho Corporation, San Diego, CA, USA). ..



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    normal human serum complement  (Quidel)
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    Quidel normal human serum complement
    ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human <t>complement.</t> Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.
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    ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human complement. Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.

    Journal: Science Advances

    Article Title: Xenopus IgX informs engineering strategies of IgM and IgG hexamers

    doi: 10.1126/sciadv.aea3737

    Figure Lengend Snippet: ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human complement. Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.

    Article Snippet: The RTX-Fcμ or RTX-Fcγ proteins were incubated with equal volumes of cell cultures (~20,000 cells) and 12% normal human serum complement (Quidel) sequentially and then transferred into a 96-microwell plate.

    Techniques: Cell Culture