Review



human complement sera  (Innovative Research Inc)


Bioz Verified Symbol Innovative Research Inc is a verified supplier
Bioz Manufacturer Symbol Innovative Research Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Innovative Research Inc human complement sera
    Optimization of the liposome composition to generate stealth liposomes. a Homogeneous <t>complement</t> assay of 10 mM SRB-encapsulating liposomes (10 µM total lipids; batches –6) showing the cholesterol dependency of liposome stealthiness in active serum (10 vol% PHS). Statistical analysis (two-sample independent t-test) revealed a significant difference between active and inactive serum samples at a cholesterol content of 10 mol% (p = 0.026) and above (p < 0.001). In contrast, no significant difference was observed at a cholesterol content of 5 mol% (p = 0.881). b Liposome stealthiness in active serum depending on SRB and cholesterol content (batches –10). 10 vol% human serum was used as a complement source (IRS41174). Statistical analysis (two-sample independent t-test) showed no significant difference between active and inactive serum samples of liposomes containing 10 mM SRB and 5 mol% cholesterol (p = 0.714), while in the other cases either a statistically significant amount of complement lysis (p < 0.001) or general serum stability was observed. Fluorescence measurements were carried out for 1 h at 37 °C in LCB, iaS, aS, and 30 mM OG + aS. Fluorescence intensities were normalized to the endpoint fluorescence of the positive control. λ Ex = 565(5 or 8) nm and λ Em = 585(5 or 8) nm; gain 150. T = 37 °C. n = 3. c Heterogeneous complement assay of biotin-modified chemiluminescence liposomes (30 mM m -carboxy luminol; batches CL1–2). Statistical analysis (two-sample independent t-test) showed a significant difference between active and inactive serum samples of high-cholesterol liposomes. Low-cholesterol liposomes remained stealth and showed no statistical difference in 5 and 10 vol% serum, whereas 25 vol% led to minor lysis in active serum. Liposomes (50 μM total lipids) were immobilized overnight on a streptavidin-coated MTP and incubated for 1 h at 37 °C in either inactive serum or active serum (5, 10, or 25 vol% PHS in LCB). Chemiluminescence measurements were performed by adding 50 μL of 4 μM hemin and 50 μL 40 mM H 2 O 2 in 0.01 M CBS, pH 10.5, 2 s integration time, gain 80, RH 1 mm, T = 25 °C, n = 3. d Flow chart of the homogeneous complement assay procedure for SRB-encapsulating liposomes
    Human Complement Sera, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+complement+plasma/Pooled+Human+Complement+Plasma/pmc12122657-67-1-14
    Average 93 stars, based on 2 article reviews
    human complement sera - by Bioz Stars, 2026-11
    93/100 stars

    Images

    1) Product Images from "A homogeneous immunoassay technology based on liposomes and the complement system enables one-step, no-wash, rapid diagnostics directly in serum"

    Article Title: A homogeneous immunoassay technology based on liposomes and the complement system enables one-step, no-wash, rapid diagnostics directly in serum

    Journal: Analytical and Bioanalytical Chemistry

    doi: 10.1007/s00216-025-05882-4

    Optimization of the liposome composition to generate stealth liposomes. a Homogeneous complement assay of 10 mM SRB-encapsulating liposomes (10 µM total lipids; batches –6) showing the cholesterol dependency of liposome stealthiness in active serum (10 vol% PHS). Statistical analysis (two-sample independent t-test) revealed a significant difference between active and inactive serum samples at a cholesterol content of 10 mol% (p = 0.026) and above (p < 0.001). In contrast, no significant difference was observed at a cholesterol content of 5 mol% (p = 0.881). b Liposome stealthiness in active serum depending on SRB and cholesterol content (batches –10). 10 vol% human serum was used as a complement source (IRS41174). Statistical analysis (two-sample independent t-test) showed no significant difference between active and inactive serum samples of liposomes containing 10 mM SRB and 5 mol% cholesterol (p = 0.714), while in the other cases either a statistically significant amount of complement lysis (p < 0.001) or general serum stability was observed. Fluorescence measurements were carried out for 1 h at 37 °C in LCB, iaS, aS, and 30 mM OG + aS. Fluorescence intensities were normalized to the endpoint fluorescence of the positive control. λ Ex = 565(5 or 8) nm and λ Em = 585(5 or 8) nm; gain 150. T = 37 °C. n = 3. c Heterogeneous complement assay of biotin-modified chemiluminescence liposomes (30 mM m -carboxy luminol; batches CL1–2). Statistical analysis (two-sample independent t-test) showed a significant difference between active and inactive serum samples of high-cholesterol liposomes. Low-cholesterol liposomes remained stealth and showed no statistical difference in 5 and 10 vol% serum, whereas 25 vol% led to minor lysis in active serum. Liposomes (50 μM total lipids) were immobilized overnight on a streptavidin-coated MTP and incubated for 1 h at 37 °C in either inactive serum or active serum (5, 10, or 25 vol% PHS in LCB). Chemiluminescence measurements were performed by adding 50 μL of 4 μM hemin and 50 μL 40 mM H 2 O 2 in 0.01 M CBS, pH 10.5, 2 s integration time, gain 80, RH 1 mm, T = 25 °C, n = 3. d Flow chart of the homogeneous complement assay procedure for SRB-encapsulating liposomes
    Figure Legend Snippet: Optimization of the liposome composition to generate stealth liposomes. a Homogeneous complement assay of 10 mM SRB-encapsulating liposomes (10 µM total lipids; batches –6) showing the cholesterol dependency of liposome stealthiness in active serum (10 vol% PHS). Statistical analysis (two-sample independent t-test) revealed a significant difference between active and inactive serum samples at a cholesterol content of 10 mol% (p = 0.026) and above (p < 0.001). In contrast, no significant difference was observed at a cholesterol content of 5 mol% (p = 0.881). b Liposome stealthiness in active serum depending on SRB and cholesterol content (batches –10). 10 vol% human serum was used as a complement source (IRS41174). Statistical analysis (two-sample independent t-test) showed no significant difference between active and inactive serum samples of liposomes containing 10 mM SRB and 5 mol% cholesterol (p = 0.714), while in the other cases either a statistically significant amount of complement lysis (p < 0.001) or general serum stability was observed. Fluorescence measurements were carried out for 1 h at 37 °C in LCB, iaS, aS, and 30 mM OG + aS. Fluorescence intensities were normalized to the endpoint fluorescence of the positive control. λ Ex = 565(5 or 8) nm and λ Em = 585(5 or 8) nm; gain 150. T = 37 °C. n = 3. c Heterogeneous complement assay of biotin-modified chemiluminescence liposomes (30 mM m -carboxy luminol; batches CL1–2). Statistical analysis (two-sample independent t-test) showed a significant difference between active and inactive serum samples of high-cholesterol liposomes. Low-cholesterol liposomes remained stealth and showed no statistical difference in 5 and 10 vol% serum, whereas 25 vol% led to minor lysis in active serum. Liposomes (50 μM total lipids) were immobilized overnight on a streptavidin-coated MTP and incubated for 1 h at 37 °C in either inactive serum or active serum (5, 10, or 25 vol% PHS in LCB). Chemiluminescence measurements were performed by adding 50 μL of 4 μM hemin and 50 μL 40 mM H 2 O 2 in 0.01 M CBS, pH 10.5, 2 s integration time, gain 80, RH 1 mm, T = 25 °C, n = 3. d Flow chart of the homogeneous complement assay procedure for SRB-encapsulating liposomes

    Techniques Used: Liposomes, Complement Assay, Lysis, Fluorescence, Positive Control, Modification, Incubation

    Homogeneous complement assay of 10 mM SRB-liposomes using antibodies as complement trigger. Time-resolved normalized fluorescence intensities of 0.5 mol% ARMS-modified liposomes (10 µM total lipids; batch ) a without or d with 0.5 mol% complement-triggering anti-ARMS antibody (A626). ARMS-liposomes were incubated with the A626 antibody for 1 h at 300 rpm. 10 vol% human serum was used as complement source (IRS35577). Time-resolved fluorescence intensities of biotinylated liposomes (1 µM total lipids; batch ) b without or e with 0.5 mol% complement-triggering anti-biotin antibody. Biotin-liposomes were incubated with the anti-biotin antibody for 1 h at 300 rpm. 5 vol% human serum was used as complement source (IRS45270). Time-resolved fluorescence intensities of PEGylated liposomes (1 µM total lipids; batch ) c without or f with 0.05 mol% complement-triggering anti-PEG antibody (clone RM105). PEG-liposomes were incubated with the anti-PEG antibody for 1 h at 300 rpm in 5 µL HSS. 5 vol% human serum was used as complement source (IRS45270). Fluorescence measurements were carried out for 1 h at 37 °C in LCB, iaS, aS and 30 mM OG + aS and normalized to the endpoint fluorescence of the positive control. λ Ex = 565(8) nm and λ Em = 585(8) nm; gain 150. T = 37 °C. n = 3
    Figure Legend Snippet: Homogeneous complement assay of 10 mM SRB-liposomes using antibodies as complement trigger. Time-resolved normalized fluorescence intensities of 0.5 mol% ARMS-modified liposomes (10 µM total lipids; batch ) a without or d with 0.5 mol% complement-triggering anti-ARMS antibody (A626). ARMS-liposomes were incubated with the A626 antibody for 1 h at 300 rpm. 10 vol% human serum was used as complement source (IRS35577). Time-resolved fluorescence intensities of biotinylated liposomes (1 µM total lipids; batch ) b without or e with 0.5 mol% complement-triggering anti-biotin antibody. Biotin-liposomes were incubated with the anti-biotin antibody for 1 h at 300 rpm. 5 vol% human serum was used as complement source (IRS45270). Time-resolved fluorescence intensities of PEGylated liposomes (1 µM total lipids; batch ) c without or f with 0.05 mol% complement-triggering anti-PEG antibody (clone RM105). PEG-liposomes were incubated with the anti-PEG antibody for 1 h at 300 rpm in 5 µL HSS. 5 vol% human serum was used as complement source (IRS45270). Fluorescence measurements were carried out for 1 h at 37 °C in LCB, iaS, aS and 30 mM OG + aS and normalized to the endpoint fluorescence of the positive control. λ Ex = 565(8) nm and λ Em = 585(8) nm; gain 150. T = 37 °C. n = 3

    Techniques Used: Complement Assay, Liposomes, Fluorescence, Modification, Incubation, Positive Control

    Dose–response curves of liposome lysis dependent on the antibody concentration. Corrected lysis values of a Biotin- (batch ) or b , c PEG-biotin-liposomes (batch ) (10 mM SRB, 1 µM total lipids) in a homogeneous complement assay performing an anti-biotin or anti-PEG (clone RM105 or clone 6.3) antibody titration. d Different combinations of these antibodies (0.03 mol% clone RM105, 0.1 mol% clone 6.3, 0.3 mol% anti-biotin antibody) with PEG biotin-liposomes (batch ). A one-way ANOVA, including a post hoc Tukey test, was performed for statistical analysis (p < 0.05) of the antibody combinations. Liposomes were incubated with the respective amount of antibodies for 1 h at 300 rpm. 5 vol% human serum was used as a complement source (IRS45270). Fluorescence intensities were adjusted by the iaS signal and normalized to the endpoint fluorescence of the positive control. λ Ex = 565(8) nm and λ Em = 585(8) nm; gain 150. T = 37 °C. n = 3
    Figure Legend Snippet: Dose–response curves of liposome lysis dependent on the antibody concentration. Corrected lysis values of a Biotin- (batch ) or b , c PEG-biotin-liposomes (batch ) (10 mM SRB, 1 µM total lipids) in a homogeneous complement assay performing an anti-biotin or anti-PEG (clone RM105 or clone 6.3) antibody titration. d Different combinations of these antibodies (0.03 mol% clone RM105, 0.1 mol% clone 6.3, 0.3 mol% anti-biotin antibody) with PEG biotin-liposomes (batch ). A one-way ANOVA, including a post hoc Tukey test, was performed for statistical analysis (p < 0.05) of the antibody combinations. Liposomes were incubated with the respective amount of antibodies for 1 h at 300 rpm. 5 vol% human serum was used as a complement source (IRS45270). Fluorescence intensities were adjusted by the iaS signal and normalized to the endpoint fluorescence of the positive control. λ Ex = 565(8) nm and λ Em = 585(8) nm; gain 150. T = 37 °C. n = 3

    Techniques Used: Lysis, Concentration Assay, Liposomes, Complement Assay, Titration, Incubation, Fluorescence, Positive Control

    Screening of anti-PEG antibodies in human sera. Corrected lysis values of PEGylated 10 mM SRB-liposomes (1 µM total lipids; batch ) in a homogeneous complement assay performing a serum titration of different commercial sera (IRS batches 31,758, 35,577, 41,174, and 45,270). 0.1–10 vol% of human sera were used. Fluorescence intensities were adjusted by the iaS signal and normalized to the endpoint fluorescence of the positive control. λ Ex = 565(8) nm and λ Em = 585(8) nm; gain 150. T = 37 °C. n = 3
    Figure Legend Snippet: Screening of anti-PEG antibodies in human sera. Corrected lysis values of PEGylated 10 mM SRB-liposomes (1 µM total lipids; batch ) in a homogeneous complement assay performing a serum titration of different commercial sera (IRS batches 31,758, 35,577, 41,174, and 45,270). 0.1–10 vol% of human sera were used. Fluorescence intensities were adjusted by the iaS signal and normalized to the endpoint fluorescence of the positive control. λ Ex = 565(8) nm and λ Em = 585(8) nm; gain 150. T = 37 °C. n = 3

    Techniques Used: Lysis, Liposomes, Complement Assay, Titration, Fluorescence, Positive Control

    Long-term storage stability study of 10 mM SRB-liposomes. Normalized fluorescence intensities of SRB-liposomes (10 µM total lipids) with different lipid compositions: a low-cholesterol, carboxylated (batch ); b low-cholesterol, PEGylated (batch ); and c high-cholesterol, biotinylated (batch ) in LCB, aS, or iaS throughout the long-term storage stability study up to 40 months at 4 °C. 10 vol% human serum was used as a complement source (PHS for 0–10 months and IRS45270 for the 40 months datapoint). Fluorescence intensities were normalized to the endpoint fluorescence of the positive control. λ Ex = 565(5) nm and λ Em = 585(5) nm; gain 150. T = 37 °C. n = 3. d Summary of the liposome storage stability at 4 °C, RT, and 37 °C
    Figure Legend Snippet: Long-term storage stability study of 10 mM SRB-liposomes. Normalized fluorescence intensities of SRB-liposomes (10 µM total lipids) with different lipid compositions: a low-cholesterol, carboxylated (batch ); b low-cholesterol, PEGylated (batch ); and c high-cholesterol, biotinylated (batch ) in LCB, aS, or iaS throughout the long-term storage stability study up to 40 months at 4 °C. 10 vol% human serum was used as a complement source (PHS for 0–10 months and IRS45270 for the 40 months datapoint). Fluorescence intensities were normalized to the endpoint fluorescence of the positive control. λ Ex = 565(5) nm and λ Em = 585(5) nm; gain 150. T = 37 °C. n = 3. d Summary of the liposome storage stability at 4 °C, RT, and 37 °C

    Techniques Used: Liposomes, Fluorescence, Positive Control

    Related Articles

    Sequencing:

    Article Title: A homogeneous immunoassay technology based on liposomes and the complement system enables one-step, no-wash, rapid diagnostics directly in serum
    Article Snippet: The phospholipids 1,2-dipalmitoyl- sn -glycero- 3-phosphocholine (DPPC), 1,2-dipalmitoyl- sn -glycero- 3-phospho-(1′-rac-glycerol) (sodium salt) (DPPG), 1,2-dimyristoyl- sn -glycero- 3-phosphoethanolamine- N -[methoxy(polyethylene glycol)− 2000] (ammonium salt) (DMPE-PEG2000), 1,2-dipalmitoyl- sn -glycero- 3-phosphoethanolamine- N -(biotinyl) (sodium salt) (DPPE-biotin) and the extruder set were purchased from Avanti Polar Lipids (Alabaster, AL, USA); 1,2-dimyristoyl- sn -glycero- 3-phosphoethanolamine- N -[biotinyl(polyethylene glycol)− 2000] (DMPE-PEG2000-biotin) was purchased from Nanocs Inc. (USA); 1,2-dipalmitoyl- sn -glycero- 3-phosphoethanolamine- N -(glutaryl) (sodium salt) ( N -glutaryl-DPPE) from Coatsome; Cholesterol (≥ 99%, C8667), N -hydroxysulfosuccinimide sodium salt (sulfo-NHS) (≥ 98%, 56,485), hemin, hydrogen peroxide solution, potassium hexacyanoferrate(II) trihydrate (≥ 98.5%, P9387), hexaamineruthenium(III) chloride (RuHex) (262,005), 2-(2-Methoxyethoxy)ethanamine (901,159; PEG-amine), bovine serum albumin fraction V (BSA), polyclonal goat anti-biotin antibody (B3640), monoclonal rabbit anti-PEG antibody clone RM105 (MABS1214), monoclonal mouse anti-PEG clone 6.3 (MABS1966), Sephadex G- 50, and human complement serum (S1764) were purchased from Sigma-Aldrich/Merck (Darmstadt, Germany); Sulforhodamine B (SRB) (S1307), (1-ethyl- 3-(3-dimethylaminopropyl) carbodiimide-hydrochloride) (EDC) (PG82079), Nunc MaxiSorp high binding microplates (437,111), polyclonal goat anti-rabbit antibody (A16098), polyclonal rabbit anti-mouse antibody (A16162) and polyclonal donkey anti-goat antibody (A16001) were purchased from Thermo Fisher Scientific (Germany); n -Octyl-β- d -glucopyranoside (OG) (≥ 98%, CN23), 2-( N -morpholino)-ethane sulphonic acid (MES) (≥ 99%, 4259), calcium chloride dihydrate (≥ 99%, 5239) and N − 2-Hydroxyethylpiperazine- N ′− 2-ethane sulphonic acid (HEPES) (≥ 99.5%, HN78) from Carl Roth (Karlsruhe, Germany); DropSens screen-printed carbon electrodes were purchased from Metrohm Germany; m -carboxy luminol (purity: 73.6 ± 2.8 wt%) was custom-made by Taros Chemicals GmbH & Co. KG (Germany). .. Pooled human complement sera (batches 31,758, 33,478, 35,577, 41,174 and 45,270) were purchased from Innovative Research (Novi, MI, USA); ARMS peptide (peptide sequence: IHTELCLPAFFSPAGTQRRFQQPQHHLTLSIIHTAAR) was purchased from ProteoGenix (France). ..

    Article Title: A homogeneous immunoassay technology based on liposomes and the complement system enables one-step, no-wash, rapid diagnostics directly in serum.
    Article Snippet: The phospholipids 1,2-dipalmitoyl-sn-glycero- 3-phosphocholine (DPPC), 1,2-dipalmitoyl-sn-glycero- 3-phospho-(1′-racglycerol) (sodium salt) (DPPG), 1,2-dimyristoyl-sn-glycero- 3-phosphoethanolamine-N-[methoxy(polyethylene glycol)− 2000] (ammonium salt) (DMPE-PEG2000), 1,2-dipalmitoyl-sn-glycero- 3-phosphoethanolamineN-(biotinyl) (sodium salt) (DPPE-biotin) and the extruder set were purchased from Avanti Polar Lipids (Alabaster, AL, USA); 1,2-dimyristoyl-sn-glycero- 3-phosphoethanolamineN-[biotinyl(polyethylene glycol)− 2000] (DMPE-PEG2000biotin) was purchased from Nanocs Inc. (USA); 1,2-dipalmitoyl-sn-glycero- 3-phosphoethanolamine-N-(glutaryl) (sodium salt) (N-glutaryl-DPPE) from Coatsome; Cholesterol (≥ 99%, C8667), N-hydroxysulfosuccinimide sodium salt (sulfo-NHS) (≥ 98%, 56,485), hemin, hydrogen peroxide solution, potassium hexacyanoferrate(II) trihydrate (≥ 98.5%, P9387), hexaamineruthenium(III) chloride (RuHex) (262,005), 2-(2-Methoxyethoxy)ethanamine (901,159; PEGamine), bovine serum albumin fraction V (BSA), polyclonal goat anti-biotin antibody (B3640), monoclonal rabbit antiPEG antibody clone RM105 (MABS1214), monoclonal mouse anti-PEG clone 6.3 (MABS1966), Sephadex G- 50, and human complement serum (S1764) were purchased from Sigma-Aldrich/Merck (Darmstadt, Germany); Sulforhodamine B (SRB) (S1307), (1-ethyl- 3-(3-dimethylaminopropyl) carbodiimide-hydrochloride) (EDC) (PG82079), Nunc MaxiSorp high binding microplates (437,111), polyclonal goat anti-rabbit antibody (A16098), polyclonal rabbit anti-mouse antibody (A16162) and polyclonal donkey anti-goat antibody (A16001) were purchased from Thermo Fisher Scientific (Germany); n-Octyl-β-d-glucopyranoside (OG) (≥ 98%, CN23), 2-(N-morpholino)-ethane sulphonic acid (MES) (≥ 99%, 4259), calcium chloride dihydrate (≥ 99%, 5239) and N− 2-Hydroxyethylpiperazine-N′− 2-ethane sulphonic acid (HEPES) (≥ 99.5%, HN78) from Carl Roth (Karlsruhe, Germany); DropSens screen-printed carbon electrodes were purchased from Metrohm Germany; m-carboxy luminol (purity: 73.6 ± 2.8 wt%) was custom-made by Taros Chemicals GmbH & Co. KG (Germany). .. Pooled human complement sera (batches 31,758, 33,478, 35,577, 41,174 and 45,270) were purchased from Innovative Research (Novi, MI, USA); ARMS peptide (peptide sequence: IHTELCLPAFFSPAGTQRRFQQPQHHLTLSIIHTAAR) was purchased from ProteoGenix (France). ..

    other:

    Article Title: HPLC-UV Method Validation for Amobarbital and Pharmaceutical Stability Evaluation When Dispersed in a Hyaluronic Acid Hydrogel: A New Concept for Post-Traumatic Osteoarthritis Prevention.
    Article Snippet: HPLC sample measurements were made using an Agilent 1100 Series HPLC station coupled with a UV−vis diode array detector (Agilent Technologies, Santa Clara, CA).



    Similar Products

    human complement sera  (Innovative Research Inc)
    93
    Innovative Research Inc human complement sera
    Human Complement Sera, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+complement+plasma/Pooled+Human+Complement+Plasma/pm40314805-41-1-14
    Average 93 stars, based on 1 article reviews
    human complement sera - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier
    iplasnac2ml  (Assaypro)
    91
    Assaypro iplasnac2ml
    Iplasnac2ml, supplied by Assaypro, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+complement+plasma/Human+Complement+Factor+H+Deficient+Plasma/pm37191558-245-60-64
    Average 91 stars, based on 1 article reviews
    iplasnac2ml - by Bioz Stars, 2026-11
    91/100 stars
    		  Buy from Supplier
    human male ab plasma as a source of human complement  (Millipore)
    90
    Millipore human male ab plasma as a source of human complement
    Anti-KLRG1 monoclonal antibodies exert no apoptosis but induce ADCP, ADCC, and <t>complement-mediated</t> cytotoxicity (CDC). A, SMZ1-WT and SMZ1-KLRG1 cells were incubated with the indicated antibodies (mAb208, 13F12F2, or its isotype control mIgG2aκ) at increasing concentrations from 1 to 100 μg/mL or 0.2 or 1 μmol/L staurosporine (positive control) for 24 hours, and the percentage of apoptotic cells was quantified by flow cytometry. Three independent experiments were conducted. Shown are the mean values of technical triplicates with error bars representing SEM from one representative experiment. B–C, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human monocyte-derived macrophages (hMDM) from three healthy donors and bone-marrow–derived murine macrophages (mBMDM) from different lots of mice in the presence of indicated antibodies for 2 hours. Anti-CD47 mAb, MIAP410-mediated phagocytosis of Jurkat cells relative to its isotype control, mIgG1κ, was used as a positive control. The percentage of CFSE + macrophages (of human CD14 + or murine CD11b + cells) was determined by flow cytometry and plotted. D–E, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human-derived PBMCs from three healthy donors in the presence of indicated antibodies at increasing concentrations for 4 hours. The percentage of propidium iodide–positive (% PI pos; of CFSE + ) cells was determined by flow cytometry and plotted. Rituximab and Raji cells were used as a positive control. F–G, ADCC reporter assay measuring luminescence with murine FcγRIIIA-expressing Jurkat cells was performed in triplicate in three independent experiments at an effector:target ratio of 25:1 with indicated antibodies. Fold induction is relative to isotype control (mIgG2aκ for mAb208 and 13F12F2). Human anti-CD20 mAb and Raji cells were used as positive control along with its isotype control, hIgG1. Representative results from one experiment are shown. H–K, CDC assay with 30% murine and human complement was performed in triplicate in three independent experiments, and % PI pos cells were reported. Rituximab and Raji cells were used as positive controls. Shown are the mean values of technical triplicates, with error bars representing SEM from one representative experiment. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by two-sided Welch t test.
    Human Male Ab Plasma As A Source Of Human Complement, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+complement+plasma/human+plasma+complement+factor+h++cfh/pmc11145167-34-8-13
    Average 90 stars, based on 1 article reviews
    human male ab plasma as a source of human complement - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    human plasma complement factor h (cfh  (Millipore)
    90
    Millipore human plasma complement factor h (cfh
    Anti-KLRG1 monoclonal antibodies exert no apoptosis but induce ADCP, ADCC, and <t>complement-mediated</t> cytotoxicity (CDC). A, SMZ1-WT and SMZ1-KLRG1 cells were incubated with the indicated antibodies (mAb208, 13F12F2, or its isotype control mIgG2aκ) at increasing concentrations from 1 to 100 μg/mL or 0.2 or 1 μmol/L staurosporine (positive control) for 24 hours, and the percentage of apoptotic cells was quantified by flow cytometry. Three independent experiments were conducted. Shown are the mean values of technical triplicates with error bars representing SEM from one representative experiment. B–C, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human monocyte-derived macrophages (hMDM) from three healthy donors and bone-marrow–derived murine macrophages (mBMDM) from different lots of mice in the presence of indicated antibodies for 2 hours. Anti-CD47 mAb, MIAP410-mediated phagocytosis of Jurkat cells relative to its isotype control, mIgG1κ, was used as a positive control. The percentage of CFSE + macrophages (of human CD14 + or murine CD11b + cells) was determined by flow cytometry and plotted. D–E, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human-derived PBMCs from three healthy donors in the presence of indicated antibodies at increasing concentrations for 4 hours. The percentage of propidium iodide–positive (% PI pos; of CFSE + ) cells was determined by flow cytometry and plotted. Rituximab and Raji cells were used as a positive control. F–G, ADCC reporter assay measuring luminescence with murine FcγRIIIA-expressing Jurkat cells was performed in triplicate in three independent experiments at an effector:target ratio of 25:1 with indicated antibodies. Fold induction is relative to isotype control (mIgG2aκ for mAb208 and 13F12F2). Human anti-CD20 mAb and Raji cells were used as positive control along with its isotype control, hIgG1. Representative results from one experiment are shown. H–K, CDC assay with 30% murine and human complement was performed in triplicate in three independent experiments, and % PI pos cells were reported. Rituximab and Raji cells were used as positive controls. Shown are the mean values of technical triplicates, with error bars representing SEM from one representative experiment. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by two-sided Welch t test.
    Human Plasma Complement Factor H (Cfh, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+complement+plasma/human+plasma+complement+factor+h++cfh/pmc10451625-76-18-25
    Average 90 stars, based on 1 article reviews
    human plasma complement factor h (cfh - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    plasma  (Assaypro)
    91
    Assaypro plasma
    Anti-KLRG1 monoclonal antibodies exert no apoptosis but induce ADCP, ADCC, and <t>complement-mediated</t> cytotoxicity (CDC). A, SMZ1-WT and SMZ1-KLRG1 cells were incubated with the indicated antibodies (mAb208, 13F12F2, or its isotype control mIgG2aκ) at increasing concentrations from 1 to 100 μg/mL or 0.2 or 1 μmol/L staurosporine (positive control) for 24 hours, and the percentage of apoptotic cells was quantified by flow cytometry. Three independent experiments were conducted. Shown are the mean values of technical triplicates with error bars representing SEM from one representative experiment. B–C, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human monocyte-derived macrophages (hMDM) from three healthy donors and bone-marrow–derived murine macrophages (mBMDM) from different lots of mice in the presence of indicated antibodies for 2 hours. Anti-CD47 mAb, MIAP410-mediated phagocytosis of Jurkat cells relative to its isotype control, mIgG1κ, was used as a positive control. The percentage of CFSE + macrophages (of human CD14 + or murine CD11b + cells) was determined by flow cytometry and plotted. D–E, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human-derived PBMCs from three healthy donors in the presence of indicated antibodies at increasing concentrations for 4 hours. The percentage of propidium iodide–positive (% PI pos; of CFSE + ) cells was determined by flow cytometry and plotted. Rituximab and Raji cells were used as a positive control. F–G, ADCC reporter assay measuring luminescence with murine FcγRIIIA-expressing Jurkat cells was performed in triplicate in three independent experiments at an effector:target ratio of 25:1 with indicated antibodies. Fold induction is relative to isotype control (mIgG2aκ for mAb208 and 13F12F2). Human anti-CD20 mAb and Raji cells were used as positive control along with its isotype control, hIgG1. Representative results from one experiment are shown. H–K, CDC assay with 30% murine and human complement was performed in triplicate in three independent experiments, and % PI pos cells were reported. Rituximab and Raji cells were used as positive controls. Shown are the mean values of technical triplicates, with error bars representing SEM from one representative experiment. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by two-sided Welch t test.
    Plasma, supplied by Assaypro, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+complement+plasma/Human+Complement+Factor+H+Deficient+Plasma/pm37191558-245-63-64
    Average 91 stars, based on 1 article reviews
    plasma - by Bioz Stars, 2026-11
    91/100 stars
    		  Buy from Supplier
    fluid phase plasma complement bb  (Quidel)
    96
    Quidel fluid phase plasma complement bb
    Anti-KLRG1 monoclonal antibodies exert no apoptosis but induce ADCP, ADCC, and <t>complement-mediated</t> cytotoxicity (CDC). A, SMZ1-WT and SMZ1-KLRG1 cells were incubated with the indicated antibodies (mAb208, 13F12F2, or its isotype control mIgG2aκ) at increasing concentrations from 1 to 100 μg/mL or 0.2 or 1 μmol/L staurosporine (positive control) for 24 hours, and the percentage of apoptotic cells was quantified by flow cytometry. Three independent experiments were conducted. Shown are the mean values of technical triplicates with error bars representing SEM from one representative experiment. B–C, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human monocyte-derived macrophages (hMDM) from three healthy donors and bone-marrow–derived murine macrophages (mBMDM) from different lots of mice in the presence of indicated antibodies for 2 hours. Anti-CD47 mAb, MIAP410-mediated phagocytosis of Jurkat cells relative to its isotype control, mIgG1κ, was used as a positive control. The percentage of CFSE + macrophages (of human CD14 + or murine CD11b + cells) was determined by flow cytometry and plotted. D–E, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human-derived PBMCs from three healthy donors in the presence of indicated antibodies at increasing concentrations for 4 hours. The percentage of propidium iodide–positive (% PI pos; of CFSE + ) cells was determined by flow cytometry and plotted. Rituximab and Raji cells were used as a positive control. F–G, ADCC reporter assay measuring luminescence with murine FcγRIIIA-expressing Jurkat cells was performed in triplicate in three independent experiments at an effector:target ratio of 25:1 with indicated antibodies. Fold induction is relative to isotype control (mIgG2aκ for mAb208 and 13F12F2). Human anti-CD20 mAb and Raji cells were used as positive control along with its isotype control, hIgG1. Representative results from one experiment are shown. H–K, CDC assay with 30% murine and human complement was performed in triplicate in three independent experiments, and % PI pos cells were reported. Rituximab and Raji cells were used as positive controls. Shown are the mean values of technical triplicates, with error bars representing SEM from one representative experiment. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by two-sided Welch t test.
    Fluid Phase Plasma Complement Bb, supplied by Quidel, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+complement+plasma/pm37093125-119-0-18
    Average 96 stars, based on 1 article reviews
    fluid phase plasma complement bb - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Anti-KLRG1 monoclonal antibodies exert no apoptosis but induce ADCP, ADCC, and complement-mediated cytotoxicity (CDC). A, SMZ1-WT and SMZ1-KLRG1 cells were incubated with the indicated antibodies (mAb208, 13F12F2, or its isotype control mIgG2aκ) at increasing concentrations from 1 to 100 μg/mL or 0.2 or 1 μmol/L staurosporine (positive control) for 24 hours, and the percentage of apoptotic cells was quantified by flow cytometry. Three independent experiments were conducted. Shown are the mean values of technical triplicates with error bars representing SEM from one representative experiment. B–C, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human monocyte-derived macrophages (hMDM) from three healthy donors and bone-marrow–derived murine macrophages (mBMDM) from different lots of mice in the presence of indicated antibodies for 2 hours. Anti-CD47 mAb, MIAP410-mediated phagocytosis of Jurkat cells relative to its isotype control, mIgG1κ, was used as a positive control. The percentage of CFSE + macrophages (of human CD14 + or murine CD11b + cells) was determined by flow cytometry and plotted. D–E, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human-derived PBMCs from three healthy donors in the presence of indicated antibodies at increasing concentrations for 4 hours. The percentage of propidium iodide–positive (% PI pos; of CFSE + ) cells was determined by flow cytometry and plotted. Rituximab and Raji cells were used as a positive control. F–G, ADCC reporter assay measuring luminescence with murine FcγRIIIA-expressing Jurkat cells was performed in triplicate in three independent experiments at an effector:target ratio of 25:1 with indicated antibodies. Fold induction is relative to isotype control (mIgG2aκ for mAb208 and 13F12F2). Human anti-CD20 mAb and Raji cells were used as positive control along with its isotype control, hIgG1. Representative results from one experiment are shown. H–K, CDC assay with 30% murine and human complement was performed in triplicate in three independent experiments, and % PI pos cells were reported. Rituximab and Raji cells were used as positive controls. Shown are the mean values of technical triplicates, with error bars representing SEM from one representative experiment. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by two-sided Welch t test.

    Journal: Clinical Cancer Research

    Article Title: KLRG1 Cell Depletion as a Novel Therapeutic Strategy in Patients with Mature T-Cell Lymphoma Subtypes

    doi: 10.1158/1078-0432.CCR-23-3504

    Figure Lengend Snippet: Anti-KLRG1 monoclonal antibodies exert no apoptosis but induce ADCP, ADCC, and complement-mediated cytotoxicity (CDC). A, SMZ1-WT and SMZ1-KLRG1 cells were incubated with the indicated antibodies (mAb208, 13F12F2, or its isotype control mIgG2aκ) at increasing concentrations from 1 to 100 μg/mL or 0.2 or 1 μmol/L staurosporine (positive control) for 24 hours, and the percentage of apoptotic cells was quantified by flow cytometry. Three independent experiments were conducted. Shown are the mean values of technical triplicates with error bars representing SEM from one representative experiment. B–C, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human monocyte-derived macrophages (hMDM) from three healthy donors and bone-marrow–derived murine macrophages (mBMDM) from different lots of mice in the presence of indicated antibodies for 2 hours. Anti-CD47 mAb, MIAP410-mediated phagocytosis of Jurkat cells relative to its isotype control, mIgG1κ, was used as a positive control. The percentage of CFSE + macrophages (of human CD14 + or murine CD11b + cells) was determined by flow cytometry and plotted. D–E, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human-derived PBMCs from three healthy donors in the presence of indicated antibodies at increasing concentrations for 4 hours. The percentage of propidium iodide–positive (% PI pos; of CFSE + ) cells was determined by flow cytometry and plotted. Rituximab and Raji cells were used as a positive control. F–G, ADCC reporter assay measuring luminescence with murine FcγRIIIA-expressing Jurkat cells was performed in triplicate in three independent experiments at an effector:target ratio of 25:1 with indicated antibodies. Fold induction is relative to isotype control (mIgG2aκ for mAb208 and 13F12F2). Human anti-CD20 mAb and Raji cells were used as positive control along with its isotype control, hIgG1. Representative results from one experiment are shown. H–K, CDC assay with 30% murine and human complement was performed in triplicate in three independent experiments, and % PI pos cells were reported. Rituximab and Raji cells were used as positive controls. Shown are the mean values of technical triplicates, with error bars representing SEM from one representative experiment. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by two-sided Welch t test.

    Article Snippet: Human male AB plasma as a source of human complement was obtained from Sigma-Aldrich.

    Techniques: Flow Cytometry Complement-Mediated Cytotoxicity Assay, Incubation, Positive Control, Flow Cytometry, Labeling, Derivative Assay, Reporter Assay, Expressing, CDC Assay