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human complement sera  (Innovative Research Inc)


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    Innovative Research Inc human complement sera
    Human Complement Sera, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+complement+plasma/Pooled+Human+Complement+Plasma/pm40314805-41-1-14
    Average 93 stars, based on 2 article reviews
    human complement sera - by Bioz Stars, 2026-10
    93/100 stars

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    Sequencing:

    Article Title: A homogeneous immunoassay technology based on liposomes and the complement system enables one-step, no-wash, rapid diagnostics directly in serum
    Article Snippet: The phospholipids 1,2-dipalmitoyl- sn -glycero- 3-phosphocholine (DPPC), 1,2-dipalmitoyl- sn -glycero- 3-phospho-(1′-rac-glycerol) (sodium salt) (DPPG), 1,2-dimyristoyl- sn -glycero- 3-phosphoethanolamine- N -[methoxy(polyethylene glycol)− 2000] (ammonium salt) (DMPE-PEG2000), 1,2-dipalmitoyl- sn -glycero- 3-phosphoethanolamine- N -(biotinyl) (sodium salt) (DPPE-biotin) and the extruder set were purchased from Avanti Polar Lipids (Alabaster, AL, USA); 1,2-dimyristoyl- sn -glycero- 3-phosphoethanolamine- N -[biotinyl(polyethylene glycol)− 2000] (DMPE-PEG2000-biotin) was purchased from Nanocs Inc. (USA); 1,2-dipalmitoyl- sn -glycero- 3-phosphoethanolamine- N -(glutaryl) (sodium salt) ( N -glutaryl-DPPE) from Coatsome; Cholesterol (≥ 99%, C8667), N -hydroxysulfosuccinimide sodium salt (sulfo-NHS) (≥ 98%, 56,485), hemin, hydrogen peroxide solution, potassium hexacyanoferrate(II) trihydrate (≥ 98.5%, P9387), hexaamineruthenium(III) chloride (RuHex) (262,005), 2-(2-Methoxyethoxy)ethanamine (901,159; PEG-amine), bovine serum albumin fraction V (BSA), polyclonal goat anti-biotin antibody (B3640), monoclonal rabbit anti-PEG antibody clone RM105 (MABS1214), monoclonal mouse anti-PEG clone 6.3 (MABS1966), Sephadex G- 50, and human complement serum (S1764) were purchased from Sigma-Aldrich/Merck (Darmstadt, Germany); Sulforhodamine B (SRB) (S1307), (1-ethyl- 3-(3-dimethylaminopropyl) carbodiimide-hydrochloride) (EDC) (PG82079), Nunc MaxiSorp high binding microplates (437,111), polyclonal goat anti-rabbit antibody (A16098), polyclonal rabbit anti-mouse antibody (A16162) and polyclonal donkey anti-goat antibody (A16001) were purchased from Thermo Fisher Scientific (Germany); n -Octyl-β- d -glucopyranoside (OG) (≥ 98%, CN23), 2-( N -morpholino)-ethane sulphonic acid (MES) (≥ 99%, 4259), calcium chloride dihydrate (≥ 99%, 5239) and N − 2-Hydroxyethylpiperazine- N ′− 2-ethane sulphonic acid (HEPES) (≥ 99.5%, HN78) from Carl Roth (Karlsruhe, Germany); DropSens screen-printed carbon electrodes were purchased from Metrohm Germany; m -carboxy luminol (purity: 73.6 ± 2.8 wt%) was custom-made by Taros Chemicals GmbH & Co. KG (Germany). .. Pooled human complement sera (batches 31,758, 33,478, 35,577, 41,174 and 45,270) were purchased from Innovative Research (Novi, MI, USA); ARMS peptide (peptide sequence: IHTELCLPAFFSPAGTQRRFQQPQHHLTLSIIHTAAR) was purchased from ProteoGenix (France). ..

    Article Title: A homogeneous immunoassay technology based on liposomes and the complement system enables one-step, no-wash, rapid diagnostics directly in serum.
    Article Snippet: The phospholipids 1,2-dipalmitoyl-sn-glycero- 3-phosphocholine (DPPC), 1,2-dipalmitoyl-sn-glycero- 3-phospho-(1′-racglycerol) (sodium salt) (DPPG), 1,2-dimyristoyl-sn-glycero- 3-phosphoethanolamine-N-[methoxy(polyethylene glycol)− 2000] (ammonium salt) (DMPE-PEG2000), 1,2-dipalmitoyl-sn-glycero- 3-phosphoethanolamineN-(biotinyl) (sodium salt) (DPPE-biotin) and the extruder set were purchased from Avanti Polar Lipids (Alabaster, AL, USA); 1,2-dimyristoyl-sn-glycero- 3-phosphoethanolamineN-[biotinyl(polyethylene glycol)− 2000] (DMPE-PEG2000biotin) was purchased from Nanocs Inc. (USA); 1,2-dipalmitoyl-sn-glycero- 3-phosphoethanolamine-N-(glutaryl) (sodium salt) (N-glutaryl-DPPE) from Coatsome; Cholesterol (≥ 99%, C8667), N-hydroxysulfosuccinimide sodium salt (sulfo-NHS) (≥ 98%, 56,485), hemin, hydrogen peroxide solution, potassium hexacyanoferrate(II) trihydrate (≥ 98.5%, P9387), hexaamineruthenium(III) chloride (RuHex) (262,005), 2-(2-Methoxyethoxy)ethanamine (901,159; PEGamine), bovine serum albumin fraction V (BSA), polyclonal goat anti-biotin antibody (B3640), monoclonal rabbit antiPEG antibody clone RM105 (MABS1214), monoclonal mouse anti-PEG clone 6.3 (MABS1966), Sephadex G- 50, and human complement serum (S1764) were purchased from Sigma-Aldrich/Merck (Darmstadt, Germany); Sulforhodamine B (SRB) (S1307), (1-ethyl- 3-(3-dimethylaminopropyl) carbodiimide-hydrochloride) (EDC) (PG82079), Nunc MaxiSorp high binding microplates (437,111), polyclonal goat anti-rabbit antibody (A16098), polyclonal rabbit anti-mouse antibody (A16162) and polyclonal donkey anti-goat antibody (A16001) were purchased from Thermo Fisher Scientific (Germany); n-Octyl-β-d-glucopyranoside (OG) (≥ 98%, CN23), 2-(N-morpholino)-ethane sulphonic acid (MES) (≥ 99%, 4259), calcium chloride dihydrate (≥ 99%, 5239) and N− 2-Hydroxyethylpiperazine-N′− 2-ethane sulphonic acid (HEPES) (≥ 99.5%, HN78) from Carl Roth (Karlsruhe, Germany); DropSens screen-printed carbon electrodes were purchased from Metrohm Germany; m-carboxy luminol (purity: 73.6 ± 2.8 wt%) was custom-made by Taros Chemicals GmbH & Co. KG (Germany). .. Pooled human complement sera (batches 31,758, 33,478, 35,577, 41,174 and 45,270) were purchased from Innovative Research (Novi, MI, USA); ARMS peptide (peptide sequence: IHTELCLPAFFSPAGTQRRFQQPQHHLTLSIIHTAAR) was purchased from ProteoGenix (France). ..

    other:

    Article Title: HPLC-UV Method Validation for Amobarbital and Pharmaceutical Stability Evaluation When Dispersed in a Hyaluronic Acid Hydrogel: A New Concept for Post-Traumatic Osteoarthritis Prevention.
    Article Snippet: HPLC sample measurements were made using an Agilent 1100 Series HPLC station coupled with a UV−vis diode array detector (Agilent Technologies, Santa Clara, CA).



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    Image Search Results


    Anti-KLRG1 monoclonal antibodies exert no apoptosis but induce ADCP, ADCC, and complement-mediated cytotoxicity (CDC). A, SMZ1-WT and SMZ1-KLRG1 cells were incubated with the indicated antibodies (mAb208, 13F12F2, or its isotype control mIgG2aκ) at increasing concentrations from 1 to 100 μg/mL or 0.2 or 1 μmol/L staurosporine (positive control) for 24 hours, and the percentage of apoptotic cells was quantified by flow cytometry. Three independent experiments were conducted. Shown are the mean values of technical triplicates with error bars representing SEM from one representative experiment. B–C, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human monocyte-derived macrophages (hMDM) from three healthy donors and bone-marrow–derived murine macrophages (mBMDM) from different lots of mice in the presence of indicated antibodies for 2 hours. Anti-CD47 mAb, MIAP410-mediated phagocytosis of Jurkat cells relative to its isotype control, mIgG1κ, was used as a positive control. The percentage of CFSE + macrophages (of human CD14 + or murine CD11b + cells) was determined by flow cytometry and plotted. D–E, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human-derived PBMCs from three healthy donors in the presence of indicated antibodies at increasing concentrations for 4 hours. The percentage of propidium iodide–positive (% PI pos; of CFSE + ) cells was determined by flow cytometry and plotted. Rituximab and Raji cells were used as a positive control. F–G, ADCC reporter assay measuring luminescence with murine FcγRIIIA-expressing Jurkat cells was performed in triplicate in three independent experiments at an effector:target ratio of 25:1 with indicated antibodies. Fold induction is relative to isotype control (mIgG2aκ for mAb208 and 13F12F2). Human anti-CD20 mAb and Raji cells were used as positive control along with its isotype control, hIgG1. Representative results from one experiment are shown. H–K, CDC assay with 30% murine and human complement was performed in triplicate in three independent experiments, and % PI pos cells were reported. Rituximab and Raji cells were used as positive controls. Shown are the mean values of technical triplicates, with error bars representing SEM from one representative experiment. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by two-sided Welch t test.

    Journal: Clinical Cancer Research

    Article Title: KLRG1 Cell Depletion as a Novel Therapeutic Strategy in Patients with Mature T-Cell Lymphoma Subtypes

    doi: 10.1158/1078-0432.CCR-23-3504

    Figure Lengend Snippet: Anti-KLRG1 monoclonal antibodies exert no apoptosis but induce ADCP, ADCC, and complement-mediated cytotoxicity (CDC). A, SMZ1-WT and SMZ1-KLRG1 cells were incubated with the indicated antibodies (mAb208, 13F12F2, or its isotype control mIgG2aκ) at increasing concentrations from 1 to 100 μg/mL or 0.2 or 1 μmol/L staurosporine (positive control) for 24 hours, and the percentage of apoptotic cells was quantified by flow cytometry. Three independent experiments were conducted. Shown are the mean values of technical triplicates with error bars representing SEM from one representative experiment. B–C, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human monocyte-derived macrophages (hMDM) from three healthy donors and bone-marrow–derived murine macrophages (mBMDM) from different lots of mice in the presence of indicated antibodies for 2 hours. Anti-CD47 mAb, MIAP410-mediated phagocytosis of Jurkat cells relative to its isotype control, mIgG1κ, was used as a positive control. The percentage of CFSE + macrophages (of human CD14 + or murine CD11b + cells) was determined by flow cytometry and plotted. D–E, CFSE-labeled SMZ1-WT and SMZ1-KLRG1 cells were incubated with human-derived PBMCs from three healthy donors in the presence of indicated antibodies at increasing concentrations for 4 hours. The percentage of propidium iodide–positive (% PI pos; of CFSE + ) cells was determined by flow cytometry and plotted. Rituximab and Raji cells were used as a positive control. F–G, ADCC reporter assay measuring luminescence with murine FcγRIIIA-expressing Jurkat cells was performed in triplicate in three independent experiments at an effector:target ratio of 25:1 with indicated antibodies. Fold induction is relative to isotype control (mIgG2aκ for mAb208 and 13F12F2). Human anti-CD20 mAb and Raji cells were used as positive control along with its isotype control, hIgG1. Representative results from one experiment are shown. H–K, CDC assay with 30% murine and human complement was performed in triplicate in three independent experiments, and % PI pos cells were reported. Rituximab and Raji cells were used as positive controls. Shown are the mean values of technical triplicates, with error bars representing SEM from one representative experiment. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by two-sided Welch t test.

    Article Snippet: Human male AB plasma as a source of human complement was obtained from Sigma-Aldrich.

    Techniques: Flow Cytometry Complement-Mediated Cytotoxicity Assay, Incubation, Positive Control, Flow Cytometry, Labeling, Derivative Assay, Reporter Assay, Expressing, CDC Assay