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preparation hprec  (ATCC)


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    ATCC preparation hprec
    Preparation Hprec, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 552 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hprec/Primary+Prostate+Epithelial+Cells%3B+Normal%2C+Human/pm40594601-252-20-22
    Average 99 stars, based on 552 article reviews
    preparation hprec - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    other:

    Article Title: Photodynamic Therapy Combined with Gemcitabine for Prostate Cancer Using Nanoprobe for in-vivo CEST Imaging
    Article Snippet: The mouse prostate cancer PNEC30 cell line was purchased from the Cell Bank of the Chinese Academy of Sciences, and the human normal prostate epithelial ATCC cell line was purchased from Wuhan Ponsure Life Science and Technology Co., Ltd.

    Article Title: N-glycome composition of extracellular vesicles released by urological cancer cells and reference non-malignant cells.
    Article Snippet: Transport of bioactive molecules via extracellular vesicles (EVs) is one of the mechanisms of intercellular communication.. EVs regulate a number of physiological processes in recipient cells, but also contribute to development and progression of many diseases, including cancer.. Many glycoproteins are incorporated into EVs, but little is known about their biological significance.

    Cell Culture:

    Article Title: Maternal helminths rewire the microbiota to promote offspring antiviral immunity.
    Article Snippet: .. Primary human bronchial epithelial cells Primary normal human bronchial epithelial (NHBE) cells were obtained from ATCC and cultured according to the supplier’s recommendations. ..

    Article Title: Salvianolic acids are natural senolytics and increase lifespan in old age
    Article Snippet: The human adipose tissue-derived mesenchymal stem cell (AT-MSC) line was from ATCC and cultured in MSC basal medium (2% FBS) with 10 ng/ml bFGF and 10 μg/ml recombinant human insulin as reported . .. The human prostate epithelial cancer cell line, PC3, was from ATCC and cultured with F-12K media (10% FBS). ..

    Expressing:

    Article Title: Bispecific antigen binding molecules that bind HER2, and methods of use thereof
    Article Snippet: .. TABLE 7 Cell Lines and Relative HER2 Expression Relative HER2 Cell Line Source Catalog # Expression Primary Myocardial Promo C12811 6% cells Cell Primary Prostate ATCC PCS-440-010 1% Epithelial cells Primary Lung ATCC PCS-300-010 1% Epithelial cells Primary Mammary ATCC PCS-600-010 1% Epithelial cells MDA-MB468 ATCC HTB-132 0% MDA-MB231 ATCC HTB-26 3% HepG2 ATCC HB-8065 13% EBC-1 6% (Lung Carcinoma) MCF7 ATCC HTB-22 15% T47D ATCC HTB-133 24% BT483 ATCC HTB121 37% ZR751 ATCC CRL-1500 39% JIMT1 DSMZ ACC589 52% MDA-MB361 ATCC HTB-27 82% MDA-MB453 ATCC HTB-131 60% SK-BR-3 ATCC HTB-30 100% B1474 ATCC HTB-20 89% N87 (NCI-N87) ATCC HTB-5822 93% Calu-3 ATCC HTB-55 84% SKOV-3 ATCC HTB-77 93% Cells were plated on collagen-coated, black wall, 96-well, optical plates (Greiner, Cat #655936) at 2.5×105 cells per well in their appropriate culture media and incubated overnight at 37° C. in 5% CO2. .. The following day, cell were incubated for 30 minutes with 10 ug/mL of Alexa647 labeled (Thermo, Cat #A37573) antibodies in 50 uL of DMEM at 4° C. After, cells were washed twice with cold DMEM+10% FBS and incubated with Δ % paraformaldehyde (Electron Microcopy Sciences, Cat #15710)+0.075% Saponin (Sigma, Cat #S4521)+10 ug/ml Hoechst (Lifetech, #H3569) in PBS for 10 minutes at room temperature.

    Multiple Displacement Amplification:

    Article Title: Bispecific antigen binding molecules that bind HER2, and methods of use thereof
    Article Snippet: .. TABLE 7 Cell Lines and Relative HER2 Expression Relative HER2 Cell Line Source Catalog # Expression Primary Myocardial Promo C12811 6% cells Cell Primary Prostate ATCC PCS-440-010 1% Epithelial cells Primary Lung ATCC PCS-300-010 1% Epithelial cells Primary Mammary ATCC PCS-600-010 1% Epithelial cells MDA-MB468 ATCC HTB-132 0% MDA-MB231 ATCC HTB-26 3% HepG2 ATCC HB-8065 13% EBC-1 6% (Lung Carcinoma) MCF7 ATCC HTB-22 15% T47D ATCC HTB-133 24% BT483 ATCC HTB121 37% ZR751 ATCC CRL-1500 39% JIMT1 DSMZ ACC589 52% MDA-MB361 ATCC HTB-27 82% MDA-MB453 ATCC HTB-131 60% SK-BR-3 ATCC HTB-30 100% B1474 ATCC HTB-20 89% N87 (NCI-N87) ATCC HTB-5822 93% Calu-3 ATCC HTB-55 84% SKOV-3 ATCC HTB-77 93% Cells were plated on collagen-coated, black wall, 96-well, optical plates (Greiner, Cat #655936) at 2.5×105 cells per well in their appropriate culture media and incubated overnight at 37° C. in 5% CO2. .. The following day, cell were incubated for 30 minutes with 10 ug/mL of Alexa647 labeled (Thermo, Cat #A37573) antibodies in 50 uL of DMEM at 4° C. After, cells were washed twice with cold DMEM+10% FBS and incubated with Δ % paraformaldehyde (Electron Microcopy Sciences, Cat #15710)+0.075% Saponin (Sigma, Cat #S4521)+10 ug/ml Hoechst (Lifetech, #H3569) in PBS for 10 minutes at room temperature.

    Incubation:

    Article Title: Bispecific antigen binding molecules that bind HER2, and methods of use thereof
    Article Snippet: .. TABLE 7 Cell Lines and Relative HER2 Expression Relative HER2 Cell Line Source Catalog # Expression Primary Myocardial Promo C12811 6% cells Cell Primary Prostate ATCC PCS-440-010 1% Epithelial cells Primary Lung ATCC PCS-300-010 1% Epithelial cells Primary Mammary ATCC PCS-600-010 1% Epithelial cells MDA-MB468 ATCC HTB-132 0% MDA-MB231 ATCC HTB-26 3% HepG2 ATCC HB-8065 13% EBC-1 6% (Lung Carcinoma) MCF7 ATCC HTB-22 15% T47D ATCC HTB-133 24% BT483 ATCC HTB121 37% ZR751 ATCC CRL-1500 39% JIMT1 DSMZ ACC589 52% MDA-MB361 ATCC HTB-27 82% MDA-MB453 ATCC HTB-131 60% SK-BR-3 ATCC HTB-30 100% B1474 ATCC HTB-20 89% N87 (NCI-N87) ATCC HTB-5822 93% Calu-3 ATCC HTB-55 84% SKOV-3 ATCC HTB-77 93% Cells were plated on collagen-coated, black wall, 96-well, optical plates (Greiner, Cat #655936) at 2.5×105 cells per well in their appropriate culture media and incubated overnight at 37° C. in 5% CO2. .. The following day, cell were incubated for 30 minutes with 10 ug/mL of Alexa647 labeled (Thermo, Cat #A37573) antibodies in 50 uL of DMEM at 4° C. After, cells were washed twice with cold DMEM+10% FBS and incubated with Δ % paraformaldehyde (Electron Microcopy Sciences, Cat #15710)+0.075% Saponin (Sigma, Cat #S4521)+10 ug/ml Hoechst (Lifetech, #H3569) in PBS for 10 minutes at room temperature.



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    Genistein and butein reduce cell viability in prostate cancer cells. ( A ) Chemical structures of GEN and BTN. ( B ) Viability of PC-3 prostate cancer cells and <t>HPrEC</t> following exposure to increasing concentrations of GEN or BTN for 24 or 48 h, evaluated using the MTT assay. ( C ) Cell viability measured by means of trypan blue exclusion under identical treatment conditions. Results are normalized to untreated controls, and IC 50 values were calculated from 48 h treatment data where indicated. Values represent the mean ± SD of at least three independent experiments; * p < 0.05 versus control. HPrEC: normal human prostate <t>epithelial</t> cells; GEN: genistein; BTN: butein; Conc.: concentration.
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    Genistein and butein reduce cell viability in prostate cancer cells. ( A ) Chemical structures of GEN and BTN. ( B ) Viability of PC-3 prostate cancer cells and <t>HPrEC</t> following exposure to increasing concentrations of GEN or BTN for 24 or 48 h, evaluated using the MTT assay. ( C ) Cell viability measured by means of trypan blue exclusion under identical treatment conditions. Results are normalized to untreated controls, and IC 50 values were calculated from 48 h treatment data where indicated. Values represent the mean ± SD of at least three independent experiments; * p < 0.05 versus control. HPrEC: normal human prostate <t>epithelial</t> cells; GEN: genistein; BTN: butein; Conc.: concentration.
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    Genistein and butein reduce cell viability in prostate cancer cells. ( A ) Chemical structures of GEN and BTN. ( B ) Viability of PC-3 prostate cancer cells and <t>HPrEC</t> following exposure to increasing concentrations of GEN or BTN for 24 or 48 h, evaluated using the MTT assay. ( C ) Cell viability measured by means of trypan blue exclusion under identical treatment conditions. Results are normalized to untreated controls, and IC 50 values were calculated from 48 h treatment data where indicated. Values represent the mean ± SD of at least three independent experiments; * p < 0.05 versus control. HPrEC: normal human prostate <t>epithelial</t> cells; GEN: genistein; BTN: butein; Conc.: concentration.
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    Genistein and butein reduce cell viability in prostate cancer cells. ( A ) Chemical structures of GEN and BTN. ( B ) Viability of PC-3 prostate cancer cells and <t>HPrEC</t> following exposure to increasing concentrations of GEN or BTN for 24 or 48 h, evaluated using the MTT assay. ( C ) Cell viability measured by means of trypan blue exclusion under identical treatment conditions. Results are normalized to untreated controls, and IC 50 values were calculated from 48 h treatment data where indicated. Values represent the mean ± SD of at least three independent experiments; * p < 0.05 versus control. HPrEC: normal human prostate <t>epithelial</t> cells; GEN: genistein; BTN: butein; Conc.: concentration.
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    Genistein and butein reduce cell viability in prostate cancer cells. ( A ) Chemical structures of GEN and BTN. ( B ) Viability of PC-3 prostate cancer cells and <t>HPrEC</t> following exposure to increasing concentrations of GEN or BTN for 24 or 48 h, evaluated using the MTT assay. ( C ) Cell viability measured by means of trypan blue exclusion under identical treatment conditions. Results are normalized to untreated controls, and IC 50 values were calculated from 48 h treatment data where indicated. Values represent the mean ± SD of at least three independent experiments; * p < 0.05 versus control. HPrEC: normal human prostate <t>epithelial</t> cells; GEN: genistein; BTN: butein; Conc.: concentration.
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    Genistein and butein reduce cell viability in prostate cancer cells. ( A ) Chemical structures of GEN and BTN. ( B ) Viability of PC-3 prostate cancer cells and <t>HPrEC</t> following exposure to increasing concentrations of GEN or BTN for 24 or 48 h, evaluated using the MTT assay. ( C ) Cell viability measured by means of trypan blue exclusion under identical treatment conditions. Results are normalized to untreated controls, and IC 50 values were calculated from 48 h treatment data where indicated. Values represent the mean ± SD of at least three independent experiments; * p < 0.05 versus control. HPrEC: normal human prostate <t>epithelial</t> cells; GEN: genistein; BTN: butein; Conc.: concentration.
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    Image Search Results


    Genistein and butein reduce cell viability in prostate cancer cells. ( A ) Chemical structures of GEN and BTN. ( B ) Viability of PC-3 prostate cancer cells and HPrEC following exposure to increasing concentrations of GEN or BTN for 24 or 48 h, evaluated using the MTT assay. ( C ) Cell viability measured by means of trypan blue exclusion under identical treatment conditions. Results are normalized to untreated controls, and IC 50 values were calculated from 48 h treatment data where indicated. Values represent the mean ± SD of at least three independent experiments; * p < 0.05 versus control. HPrEC: normal human prostate epithelial cells; GEN: genistein; BTN: butein; Conc.: concentration.

    Journal: Current Issues in Molecular Biology

    Article Title: Genistein–Butein Co-Treatment Suppresses Glycolytic Metabolism and Induces Apoptotic Signaling in PC-3 Prostate Cancer Cells

    doi: 10.3390/cimb48030258

    Figure Lengend Snippet: Genistein and butein reduce cell viability in prostate cancer cells. ( A ) Chemical structures of GEN and BTN. ( B ) Viability of PC-3 prostate cancer cells and HPrEC following exposure to increasing concentrations of GEN or BTN for 24 or 48 h, evaluated using the MTT assay. ( C ) Cell viability measured by means of trypan blue exclusion under identical treatment conditions. Results are normalized to untreated controls, and IC 50 values were calculated from 48 h treatment data where indicated. Values represent the mean ± SD of at least three independent experiments; * p < 0.05 versus control. HPrEC: normal human prostate epithelial cells; GEN: genistein; BTN: butein; Conc.: concentration.

    Article Snippet: Human prostate cancer PC-3 cells and normal human prostate epithelial cells (HPrEC) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA).

    Techniques: MTT Assay, Control, Concentration Assay

    Combined genistein and butein treatment impairs glycolytic metabolism in PC-3 cells. HPrEC and PC-3 cells were treated with GEN, BTN, or GEN/BTN for 48 h. ( A , B ) Extracellular lactate production and glucose consumption were quantified and normalized to control levels. Enzymatic activities of hexokinase (HK) and pyruvate dehydrogenase (PDH) were assessed following treatment. GEN/BTN co-treatment produced a pronounced suppression of glycolytic activity in PC-3 cells, whereas metabolic parameters in HPrEC cells remained largely unchanged. Data are shown as the mean ± SD (n = 3). Statistical significance is indicated by asterisks (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001). HPrEC: normal human prostate epithelial cells; GEN: genistein; BTN: butein.

    Journal: Current Issues in Molecular Biology

    Article Title: Genistein–Butein Co-Treatment Suppresses Glycolytic Metabolism and Induces Apoptotic Signaling in PC-3 Prostate Cancer Cells

    doi: 10.3390/cimb48030258

    Figure Lengend Snippet: Combined genistein and butein treatment impairs glycolytic metabolism in PC-3 cells. HPrEC and PC-3 cells were treated with GEN, BTN, or GEN/BTN for 48 h. ( A , B ) Extracellular lactate production and glucose consumption were quantified and normalized to control levels. Enzymatic activities of hexokinase (HK) and pyruvate dehydrogenase (PDH) were assessed following treatment. GEN/BTN co-treatment produced a pronounced suppression of glycolytic activity in PC-3 cells, whereas metabolic parameters in HPrEC cells remained largely unchanged. Data are shown as the mean ± SD (n = 3). Statistical significance is indicated by asterisks (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001). HPrEC: normal human prostate epithelial cells; GEN: genistein; BTN: butein.

    Article Snippet: Human prostate cancer PC-3 cells and normal human prostate epithelial cells (HPrEC) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA).

    Techniques: Control, Produced, Activity Assay

    Genistein–butein co-treatment decreases ATP levels and clonogenic survival in PC-3 cells. ( A ) Intracellular ATP content was determined after 48 h exposure to GEN, BTN, or GEN/BTN, and expressed relative to control cells (n = 3 independent experiments). ( B ) Crystal violet staining images illustrating treatment-dependent changes in cell survival. ( C ) Quantitative analysis of crystal violet staining measured at 590 nm. Each dot represents an individual replicate, and data are presented as mean ± SD (total n = 8 pooled from at least three independent experiments). Following GEN/BTN co-treatment in PC-3 cells, a significant reduction in ATP production and cell survival was observed, while normal HPrEC cells showed minimal changes. Results represent the mean ± SD from three independent experiments. Statistical significance is indicated by asterisks (* p < 0.05; ** p < 0.01; **** p < 0.0001). HPrEC: normal human prostate epithelial cells; GEN: genistein; BTN: butein.

    Journal: Current Issues in Molecular Biology

    Article Title: Genistein–Butein Co-Treatment Suppresses Glycolytic Metabolism and Induces Apoptotic Signaling in PC-3 Prostate Cancer Cells

    doi: 10.3390/cimb48030258

    Figure Lengend Snippet: Genistein–butein co-treatment decreases ATP levels and clonogenic survival in PC-3 cells. ( A ) Intracellular ATP content was determined after 48 h exposure to GEN, BTN, or GEN/BTN, and expressed relative to control cells (n = 3 independent experiments). ( B ) Crystal violet staining images illustrating treatment-dependent changes in cell survival. ( C ) Quantitative analysis of crystal violet staining measured at 590 nm. Each dot represents an individual replicate, and data are presented as mean ± SD (total n = 8 pooled from at least three independent experiments). Following GEN/BTN co-treatment in PC-3 cells, a significant reduction in ATP production and cell survival was observed, while normal HPrEC cells showed minimal changes. Results represent the mean ± SD from three independent experiments. Statistical significance is indicated by asterisks (* p < 0.05; ** p < 0.01; **** p < 0.0001). HPrEC: normal human prostate epithelial cells; GEN: genistein; BTN: butein.

    Article Snippet: Human prostate cancer PC-3 cells and normal human prostate epithelial cells (HPrEC) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA).

    Techniques: Control, Staining

    Genistein–butein co-treatment promotes apoptosis and suppresses AKT/ERK signaling in PC-3 cells. ( A ) Apoptotic cell populations were evaluated using Annexin V and viability staining after 48 h treatment. Representative dot plots are shown for HPrEC and PC-3 cells. ( B ) Expression levels of hexokinase II and PDH were analyzed by means of Western blotting, with β-actin serving as a loading control. Corresponding densitometric analyses are presented. ( C ) Phosphorylation status of AKT and ERK, along with levels of cleaved caspase-3 and cleaved PARP, was examined by Western blot analysis. GEN/BTN co-treatment selectively inhibited survival signaling and enhanced apoptotic marker cleavage in PC-3 cells. Data are presented as the mean ± SD. Statistical significance is indicated. Statistical significance is indicated by asterisks (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001). HPrEC: normal human prostate epithelial cells; GEN: genistein; BTN: butein; PDH: pyruvate dehydrogenase.

    Journal: Current Issues in Molecular Biology

    Article Title: Genistein–Butein Co-Treatment Suppresses Glycolytic Metabolism and Induces Apoptotic Signaling in PC-3 Prostate Cancer Cells

    doi: 10.3390/cimb48030258

    Figure Lengend Snippet: Genistein–butein co-treatment promotes apoptosis and suppresses AKT/ERK signaling in PC-3 cells. ( A ) Apoptotic cell populations were evaluated using Annexin V and viability staining after 48 h treatment. Representative dot plots are shown for HPrEC and PC-3 cells. ( B ) Expression levels of hexokinase II and PDH were analyzed by means of Western blotting, with β-actin serving as a loading control. Corresponding densitometric analyses are presented. ( C ) Phosphorylation status of AKT and ERK, along with levels of cleaved caspase-3 and cleaved PARP, was examined by Western blot analysis. GEN/BTN co-treatment selectively inhibited survival signaling and enhanced apoptotic marker cleavage in PC-3 cells. Data are presented as the mean ± SD. Statistical significance is indicated. Statistical significance is indicated by asterisks (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001). HPrEC: normal human prostate epithelial cells; GEN: genistein; BTN: butein; PDH: pyruvate dehydrogenase.

    Article Snippet: Human prostate cancer PC-3 cells and normal human prostate epithelial cells (HPrEC) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA).

    Techniques: Staining, Expressing, Western Blot, Control, Phospho-proteomics, Marker