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inverted microscope using hoffman modulation contrast  (Nikon)


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    Nikon inverted microscope using hoffman modulation contrast
    Inverted Microscope Using Hoffman Modulation Contrast, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hoffman+modulation+contrast+microscope/pmc08669404-97-15-22
    Average 90 stars, based on 1 article reviews
    inverted microscope using hoffman modulation contrast - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: SLC7A1 , SGK1 and HMGB2 are overexpressed in cervical cancer tissues and the miR-23b-3p/HMGB2 axis regulates cell migration and invasion
    Article Snippet: Images of invading cells were obtained with an inverted microscope (Eclipse TS2; Nikon Corporation) with 10X objective.

    Article Title: Transcriptomic signatures of neonatal acute respiratory distress syndrome in a prospective cohort of respiratory distress
    Article Snippet: Images were captured using a Nikon DS-U microscope (Tokyo, Japan).

    Article Title: A novel defined manganese metabolism-related gene signature for predicting the prognosis of pancreatic ductal adenocarcinoma
    Article Snippet: Wound closure was observed under a microscope (Nikon Eclipse Ts2; Nikon Corporation) and images were captured at 0, 24 and 48 h post-scratch.

    Article Title: Inhibition of gastric adenocarcinoma proliferation by WSGC@MS: Role of KEAP1/NRF2 signaling pathway and autophagy regulation
    Article Snippet: The TUNEL assay was performed using the TUNEL apoptosis detection kit (S7100, Sigma-Aldrich, USA) following the manufacturer's instructions to confirm in situ cell apoptosis on 5 μm-thick paraffin sections.

    Article Title: SLC7A1 , SGK1 and HMGB2 are overexpressed in cervical cancer tissues and the miR-23b-3p/HMGB2 axis regulates cell migration and invasion
    Article Snippet: The culture medium was replaced every 24 h. Images of wound closure were captured with an inverted microscope (Eclipse TS2; Nikon Corporation) at magnification, ×10 at 0, 12, 24, 24, 36 and 48 h after scratching.

    Article Title: Integrative machine learning reveals the biological function and prognostic significance of α-ketoglutarate in gastric cancer
    Article Snippet: Flow cytometric data were analysed using FlowJo software (version 10.8.1; BD Biosciences, http://www.flowjo.com/ ).

    Article Title: M Ⅱ -polyphosphate-based wax-like material with osteogenesis and blood occlusion
    Article Snippet: After 14 days of cultivation, calcium deposition of h-BMSCs was detected using Alizarin red staining (Cyagen, HUXMA-90,021, China).

    Microscopy:

    Article Title: Sulfhydryl-functionalized anisotropic photonic crystal hydrogels for visual Hg 2+ detection and adsorption in cinnabar mine water area.
    Article Snippet: Emissions of mercury ions from human activities during mining and smelting processes cannot be overlooked due to their potential for environmental pollution.. Consequently, developing a material that offers both visual detection capability and efficient adsorption for Hg2+ is crucial.. Inspired by cephalopod skin, we have prepared a sulfhydryl-functionalized anisotropic photonic crystal hydrogel (PDGI/PAAm-SH).



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    Knockdown of LEDGF/p75, JPO2, menin, or HRP2 impairs tumorsphere formation in PC3-DR and DU145-DR cells. siRNA-mediated knockdowns of LEDGF/p75 ( A ), JPO2 ( B ), menin ( C ), and HRP2 ( D ) were confirmed by immunoblotting in PC3-DR and DU145-DR cells transfected with specific siRNAs (KD) compared to cells transfected with SD control oligos. Representative images of tumorsphere formation assays showing a decrease in spheroid size in PC3-DR and DU145-DR cells after individual siRNA-mediated depletion of LEDGF/p75, JPO2, menin, or HRP2 compared to SD control spheres. Tumorspheres were visualized using phase contrast or <t>Hoffman</t> <t>Modulation</t> Contrast microscopy. Tumorsphere area was quantified (bottom graphs) from triplicate images per experiment using ImageJ software. Data are from at least 3 independent experiments and are represented as mean SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001.
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    inverted microscope using hoffman modulation contrast  (Nikon)
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    Nikon inverted microscope using hoffman modulation contrast
    Knockdown of LEDGF/p75, JPO2, menin, or HRP2 impairs tumorsphere formation in PC3-DR and DU145-DR cells. siRNA-mediated knockdowns of LEDGF/p75 ( A ), JPO2 ( B ), menin ( C ), and HRP2 ( D ) were confirmed by immunoblotting in PC3-DR and DU145-DR cells transfected with specific siRNAs (KD) compared to cells transfected with SD control oligos. Representative images of tumorsphere formation assays showing a decrease in spheroid size in PC3-DR and DU145-DR cells after individual siRNA-mediated depletion of LEDGF/p75, JPO2, menin, or HRP2 compared to SD control spheres. Tumorspheres were visualized using phase contrast or <t>Hoffman</t> <t>Modulation</t> Contrast microscopy. Tumorsphere area was quantified (bottom graphs) from triplicate images per experiment using ImageJ software. Data are from at least 3 independent experiments and are represented as mean SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001.
    Inverted Microscope Using Hoffman Modulation Contrast, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Knockdown of LEDGF/p75, JPO2, menin, or HRP2 impairs tumorsphere formation in PC3-DR and DU145-DR cells. siRNA-mediated knockdowns of LEDGF/p75 ( A ), JPO2 ( B ), menin ( C ), and HRP2 ( D ) were confirmed by immunoblotting in PC3-DR and DU145-DR cells transfected with specific siRNAs (KD) compared to cells transfected with SD control oligos. Representative images of tumorsphere formation assays showing a decrease in spheroid size in PC3-DR and DU145-DR cells after individual siRNA-mediated depletion of LEDGF/p75, JPO2, menin, or HRP2 compared to SD control spheres. Tumorspheres were visualized using phase contrast or Hoffman Modulation Contrast microscopy. Tumorsphere area was quantified (bottom graphs) from triplicate images per experiment using ImageJ software. Data are from at least 3 independent experiments and are represented as mean SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001.

    Journal: Cells

    Article Title: The LEDGF/p75 Integrase Binding Domain Interactome Contributes to the Survival, Clonogenicity, and Tumorsphere Formation of Docetaxel-Resistant Prostate Cancer Cells

    doi: 10.3390/cells10102723

    Figure Lengend Snippet: Knockdown of LEDGF/p75, JPO2, menin, or HRP2 impairs tumorsphere formation in PC3-DR and DU145-DR cells. siRNA-mediated knockdowns of LEDGF/p75 ( A ), JPO2 ( B ), menin ( C ), and HRP2 ( D ) were confirmed by immunoblotting in PC3-DR and DU145-DR cells transfected with specific siRNAs (KD) compared to cells transfected with SD control oligos. Representative images of tumorsphere formation assays showing a decrease in spheroid size in PC3-DR and DU145-DR cells after individual siRNA-mediated depletion of LEDGF/p75, JPO2, menin, or HRP2 compared to SD control spheres. Tumorspheres were visualized using phase contrast or Hoffman Modulation Contrast microscopy. Tumorsphere area was quantified (bottom graphs) from triplicate images per experiment using ImageJ software. Data are from at least 3 independent experiments and are represented as mean SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001.

    Article Snippet: Tumorspheres were grown for 4 days at 37 °C/5% CO 2 and visualized in an Olympus IX70 microscope equipped with Phase Contrast and Hoffman Modulation Contrast, and a SPOT imaging system.

    Techniques: Western Blot, Transfection, Microscopy, Software