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inverted microscope hoffman modulation contrast equipment  (Nikon)


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    Nikon inverted microscope hoffman modulation contrast equipment
    Inverted Microscope Hoffman Modulation Contrast Equipment, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hoffman+modulation+contrast+microscope/te2000/pmc04793165-72-26-30
    Average 90 stars, based on 1 article reviews
    inverted microscope hoffman modulation contrast equipment - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Injection:

    Article Title: A force-sensitive mutation reveals a non-canonical role for dynein in anaphase progression
    Article Snippet: Colchicine (Stratech Scientific) was dissolved in DMSO to make a stock solution of 100 mg/ml, which was diluted on the day of the experiment to 1 mg/ml with dH 2 O. Embryos from a 60-min egg lay were aged for a further 60 min before being dechorionated and lined up in rows on an apple juice plate with a fine paintbrush, transferred to a 9 × 35-mm coverslip coated with heptane glue, and desiccated for 4–5 min with silica gel (Sigma-Aldrich). .. 1.5–2.5 h after egg laying, embryos were injected with ∼100 pl of either 1 mg/ml colchicine or 1% DMSO (vehicle control) using a laser-pulled borosilicate glass needle mounted on a Narishige micromanipulator that was attached to a Nikon Eclipse TS100 microscope. .. 1.5–2.5 h after egg laying, embryos were injected with ∼100 pl of either 1 mg/ml colchicine or 1% DMSO (vehicle control) using a laser-pulled borosilicate glass needle mounted on a Narishige micromanipulator that was attached to a Nikon Eclipse TS100 microscope.

    Control:

    Article Title: A force-sensitive mutation reveals a non-canonical role for dynein in anaphase progression
    Article Snippet: Colchicine (Stratech Scientific) was dissolved in DMSO to make a stock solution of 100 mg/ml, which was diluted on the day of the experiment to 1 mg/ml with dH 2 O. Embryos from a 60-min egg lay were aged for a further 60 min before being dechorionated and lined up in rows on an apple juice plate with a fine paintbrush, transferred to a 9 × 35-mm coverslip coated with heptane glue, and desiccated for 4–5 min with silica gel (Sigma-Aldrich). .. 1.5–2.5 h after egg laying, embryos were injected with ∼100 pl of either 1 mg/ml colchicine or 1% DMSO (vehicle control) using a laser-pulled borosilicate glass needle mounted on a Narishige micromanipulator that was attached to a Nikon Eclipse TS100 microscope. .. 1.5–2.5 h after egg laying, embryos were injected with ∼100 pl of either 1 mg/ml colchicine or 1% DMSO (vehicle control) using a laser-pulled borosilicate glass needle mounted on a Narishige micromanipulator that was attached to a Nikon Eclipse TS100 microscope.

    Microscopy:

    Article Title: A force-sensitive mutation reveals a non-canonical role for dynein in anaphase progression
    Article Snippet: Colchicine (Stratech Scientific) was dissolved in DMSO to make a stock solution of 100 mg/ml, which was diluted on the day of the experiment to 1 mg/ml with dH 2 O. Embryos from a 60-min egg lay were aged for a further 60 min before being dechorionated and lined up in rows on an apple juice plate with a fine paintbrush, transferred to a 9 × 35-mm coverslip coated with heptane glue, and desiccated for 4–5 min with silica gel (Sigma-Aldrich). .. 1.5–2.5 h after egg laying, embryos were injected with ∼100 pl of either 1 mg/ml colchicine or 1% DMSO (vehicle control) using a laser-pulled borosilicate glass needle mounted on a Narishige micromanipulator that was attached to a Nikon Eclipse TS100 microscope. .. 1.5–2.5 h after egg laying, embryos were injected with ∼100 pl of either 1 mg/ml colchicine or 1% DMSO (vehicle control) using a laser-pulled borosilicate glass needle mounted on a Narishige micromanipulator that was attached to a Nikon Eclipse TS100 microscope.

    Article Title: Comapping Cellular Content and Extracellular Matrix with Hemodynamics in Intact Arterial Tissues Using Scanning Immunofluorescent Multiphoton Microscopy
    Article Snippet: Scanned images were reconstructed with IMARIS 9.5.0 (BitPlane AG, Zurich, Switzerland). .. Imaging with scanning multiphoton microscope A scanning multiphoton microscope (Nikon A1R MP HD, Tokyo, Japan) with the Nikon Ni-E upright motorized system and Chameleon Laser vision was used for large area scans of the artery and aneurysm specimens as well as for 500 μm x 500 μm scans of sectioned artery tissue (scanning feature not used). ..

    Article Title: Click chemistry-mediated enrichment of circulating tumor cells and tumor-derived extracellular vesicles for dual liquid biopsy in differentiated thyroid cancer
    Article Snippet: Circulating tumor cells (CTCs) and tumor-derived extracellular vesicles (tEVs) are two crucial methodologies of liquid biopsy.. Given their distinct size differences and release dynamics, CTCs and tEVs potentially offer synergistic capabilities in the non-invasive detection of differentiated thyroid cancer (DTC), a typically indolent tumor.. We present the Combined DTC CTC/tEV Assay, integrating dual liquid biopsy processes: i) DTC CTC enrichment by Click Chips, followed by analysis of seven DTC-specific genes, and ii) DTC tEV enrichment by Click Beads, succeeded by mRNA cargo quantification in DTC tEVs.

    other:

    Article Title: Efficacy of Platelet-rich Plasma Versus Platelet-rich Fibrin Versus Conventional Treatment in Chronic Non-healing Skin Ulcers: A Comparative Study
    Article Snippet: Outcome Measure: Objective assessment of ulcer size for effectiveness, using Image J software.. Measure Description: Photographs will be obtained at baseline, one month after and at end of eight sessions (two months) with a Nikon D3300 AF-P 18-55 VR 24.2 Megapixels® digital camera and will be standardized for light and distance from the ulcer.. Time Frame: Four months of follow up after eight sessions of treatment (six months total)

    Article Title: pHusion – a robust and versatile toolset for automated detection and analysis of exocytosis
    Article Snippet: The system was equipped with a Nikon Perfect Focus System (PFS) and images were acquired with the NIS-Elements AR 5.30.05 software.

    Article Title: Early endocannabinoid-mediated depolarization-induced suppression of excitation delays the appearance of the epileptic phenotype in synapsin II knockout mice
    Article Snippet: The immunostained sections were observed with a Nikon Eclipse Ti2 confocal microscope (Nikon, Florence, Italy) equipped with an x–y–z motorized stage, a digital camera DS-Qi2 and the acquisition and image analysis software NIS-Elements C (Nikon, The Netherlands).

    Article Title: A spatiotemporal “bulk erosion” mode in selective laser melted magnesium alloys and the resulting adverse cell & tissue responses
    Article Snippet: Selective laser melting (SLM) has attracted great attention in the fabrication of magnesium-based biodegradable implants.. However, current SLMed magnesium alloys are generally suffered from rapid corrosion, which is deadly detrimental to their use.. Herein, we thoroughly revealed why they are so vulnerable to corrosion through a typical SLMed AZ91D material model. An abnormally spatiotemporal “bulk erosion” mechanism was found, not the well-known “surface corrosion” mode of traditionally plastic-deformed alloys.

    Imaging:

    Article Title: Comapping Cellular Content and Extracellular Matrix with Hemodynamics in Intact Arterial Tissues Using Scanning Immunofluorescent Multiphoton Microscopy
    Article Snippet: Scanned images were reconstructed with IMARIS 9.5.0 (BitPlane AG, Zurich, Switzerland). .. Imaging with scanning multiphoton microscope A scanning multiphoton microscope (Nikon A1R MP HD, Tokyo, Japan) with the Nikon Ni-E upright motorized system and Chameleon Laser vision was used for large area scans of the artery and aneurysm specimens as well as for 500 μm x 500 μm scans of sectioned artery tissue (scanning feature not used). ..

    Fluorescence:

    Article Title: Click chemistry-mediated enrichment of circulating tumor cells and tumor-derived extracellular vesicles for dual liquid biopsy in differentiated thyroid cancer
    Article Snippet: Circulating tumor cells (CTCs) and tumor-derived extracellular vesicles (tEVs) are two crucial methodologies of liquid biopsy.. Given their distinct size differences and release dynamics, CTCs and tEVs potentially offer synergistic capabilities in the non-invasive detection of differentiated thyroid cancer (DTC), a typically indolent tumor.. We present the Combined DTC CTC/tEV Assay, integrating dual liquid biopsy processes: i) DTC CTC enrichment by Click Chips, followed by analysis of seven DTC-specific genes, and ii) DTC tEV enrichment by Click Beads, succeeded by mRNA cargo quantification in DTC tEVs.



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    Knockdown of LEDGF/p75, JPO2, menin, or HRP2 impairs tumorsphere formation in PC3-DR and DU145-DR cells. siRNA-mediated knockdowns of LEDGF/p75 ( A ), JPO2 ( B ), menin ( C ), and HRP2 ( D ) were confirmed by immunoblotting in PC3-DR and DU145-DR cells transfected with specific siRNAs (KD) compared to cells transfected with SD control oligos. Representative images of tumorsphere formation assays showing a decrease in spheroid size in PC3-DR and DU145-DR cells after individual siRNA-mediated depletion of LEDGF/p75, JPO2, menin, or HRP2 compared to SD control spheres. Tumorspheres were visualized using phase contrast or <t>Hoffman</t> <t>Modulation</t> Contrast microscopy. Tumorsphere area was quantified (bottom graphs) from triplicate images per experiment using ImageJ software. Data are from at least 3 independent experiments and are represented as mean SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001.
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    Knockdown of LEDGF/p75, JPO2, menin, or HRP2 impairs tumorsphere formation in PC3-DR and DU145-DR cells. siRNA-mediated knockdowns of LEDGF/p75 ( A ), JPO2 ( B ), menin ( C ), and HRP2 ( D ) were confirmed by immunoblotting in PC3-DR and DU145-DR cells transfected with specific siRNAs (KD) compared to cells transfected with SD control oligos. Representative images of tumorsphere formation assays showing a decrease in spheroid size in PC3-DR and DU145-DR cells after individual siRNA-mediated depletion of LEDGF/p75, JPO2, menin, or HRP2 compared to SD control spheres. Tumorspheres were visualized using phase contrast or <t>Hoffman</t> <t>Modulation</t> Contrast microscopy. Tumorsphere area was quantified (bottom graphs) from triplicate images per experiment using ImageJ software. Data are from at least 3 independent experiments and are represented as mean SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001.
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    Knockdown of LEDGF/p75, JPO2, menin, or HRP2 impairs tumorsphere formation in PC3-DR and DU145-DR cells. siRNA-mediated knockdowns of LEDGF/p75 ( A ), JPO2 ( B ), menin ( C ), and HRP2 ( D ) were confirmed by immunoblotting in PC3-DR and DU145-DR cells transfected with specific siRNAs (KD) compared to cells transfected with SD control oligos. Representative images of tumorsphere formation assays showing a decrease in spheroid size in PC3-DR and DU145-DR cells after individual siRNA-mediated depletion of LEDGF/p75, JPO2, menin, or HRP2 compared to SD control spheres. Tumorspheres were visualized using phase contrast or <t>Hoffman</t> <t>Modulation</t> Contrast microscopy. Tumorsphere area was quantified (bottom graphs) from triplicate images per experiment using ImageJ software. Data are from at least 3 independent experiments and are represented as mean SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001.
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    Image Search Results


    Knockdown of LEDGF/p75, JPO2, menin, or HRP2 impairs tumorsphere formation in PC3-DR and DU145-DR cells. siRNA-mediated knockdowns of LEDGF/p75 ( A ), JPO2 ( B ), menin ( C ), and HRP2 ( D ) were confirmed by immunoblotting in PC3-DR and DU145-DR cells transfected with specific siRNAs (KD) compared to cells transfected with SD control oligos. Representative images of tumorsphere formation assays showing a decrease in spheroid size in PC3-DR and DU145-DR cells after individual siRNA-mediated depletion of LEDGF/p75, JPO2, menin, or HRP2 compared to SD control spheres. Tumorspheres were visualized using phase contrast or Hoffman Modulation Contrast microscopy. Tumorsphere area was quantified (bottom graphs) from triplicate images per experiment using ImageJ software. Data are from at least 3 independent experiments and are represented as mean SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001.

    Journal: Cells

    Article Title: The LEDGF/p75 Integrase Binding Domain Interactome Contributes to the Survival, Clonogenicity, and Tumorsphere Formation of Docetaxel-Resistant Prostate Cancer Cells

    doi: 10.3390/cells10102723

    Figure Lengend Snippet: Knockdown of LEDGF/p75, JPO2, menin, or HRP2 impairs tumorsphere formation in PC3-DR and DU145-DR cells. siRNA-mediated knockdowns of LEDGF/p75 ( A ), JPO2 ( B ), menin ( C ), and HRP2 ( D ) were confirmed by immunoblotting in PC3-DR and DU145-DR cells transfected with specific siRNAs (KD) compared to cells transfected with SD control oligos. Representative images of tumorsphere formation assays showing a decrease in spheroid size in PC3-DR and DU145-DR cells after individual siRNA-mediated depletion of LEDGF/p75, JPO2, menin, or HRP2 compared to SD control spheres. Tumorspheres were visualized using phase contrast or Hoffman Modulation Contrast microscopy. Tumorsphere area was quantified (bottom graphs) from triplicate images per experiment using ImageJ software. Data are from at least 3 independent experiments and are represented as mean SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001.

    Article Snippet: Tumorspheres were grown for 4 days at 37 °C/5% CO 2 and visualized in an Olympus IX70 microscope equipped with Phase Contrast and Hoffman Modulation Contrast, and a SPOT imaging system.

    Techniques: Western Blot, Transfection, Microscopy, Software