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Beijing Genomics Institute Shenzhen high-throughput whole transcriptome sequencing
High Throughput Whole Transcriptome Sequencing, supplied by Beijing Genomics Institute Shenzhen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+throughput+transcriptome+sequencing/transcriptome+sequencing/pm40659783-62-0-13
Average 90 stars, based on 1 article reviews
high-throughput whole transcriptome sequencing - by Bioz Stars, 2026-09
90/100 stars

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Sequencing:

Article Title: TBK1 phagosomal recruitment enhances antifungal immunity via positive feedback regulation with SRC.
Article Snippet: .. Transcriptome sequencing was conducted by BGI (Beijing Genomics Institute). .. In brief, RNA was extracted using the TRIzol method,57 and the integrity of the extracted RNA was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologie).

Article Title: RN7SL1 overexpression promotes cell proliferation in cutaneous T-cell lymphoma via miR-34a-5p/MYCN axis.
Article Snippet: Background: Cutaneous T-cell lymphoma (CTCL) is a type of lymphoma that presents in skin tissue without evidence of extracutaneous disease.. Emerging evidence indicates that long noncoding RNA-RN7SL1 serves as crucial effectors in modulating progression of different malignancies, including breast cancer, liver cancer, and other neoplasms.. Objective: To figure out the role of RN7SL1 in the pathogenesis of CTCL.

Article Title: Nematocidal activity of the oomycete Lagenidium juracyae against pine wood nematode.
Article Snippet: .. 2.4.1 RNA preparation and transcriptome sequencing Transcriptome sequencing was conducted with the assistance of the Beijing Genomics Institute (BGI, Shenzhen, China). .. Total RNA was extracted from the frozen pine wood nematodes using the RNAprep kit (Tiangen, Beijing, China) with three biological replicates and then purified further with the RNA clean kit (Tiangen, Beijing, China), according to the manufacturer's instructions.

Article Title: Genome-Wide Identification and Characterization of the Class III Peroxidase Gene Family in Radish ( Raphanus sativus ) with Insights into Their Roles in Anthocyanin Metabolism.
Article Snippet: .. Transcriptome sequencing of the tissues of the chimeric-colored radish mutant was completed by BGI (Beijing Genomics Institute, Shenzhen, China). ..

Article Title: Schisandrin B targets CDK4/6 to suppress proliferation and enhance radiosensitivity in nasopharyngeal carcinoma by inducing cell cycle arrest.
Article Snippet: 250 μL PI/RNase (Roche, Shanghai, China) was added, following by incubation in dark at room temperature for 15 min. After screening, cell cycle distribution was analyzed by a BD flow cytometer (Franklin Lakes, NJ, USA). .. Transcriptome sequencing and differential expression analysis We prepared samples of HONE-1, HONE-1-SchB, NP69, and NP69-SchB to send to the Beijing Genomics Institute (BGI) for sequencing (Drug effects for 48 h), and sequencing was repeated three times. ..

Article Title: The complete synthetic pathway of echinacoside from Cistanche deserticola and its de novo biosynthesis in yeast.
Article Snippet: The medium used was 1/2 MS 482 medium supplemented with 20 g/L sucrose, 500 mg/L amicase, and 0.6 g/L activated 483 charcoal, with pH adjusted to 5.6-6.0, and sterilized at 115°C for 20 min. 484 Transcriptome sequencing and analysis of sampled plants and callus of C. 485 deserticola 486 After high-performance liquid chromatography (HPLC) analysis of all samples from plant 487 samples and callus samples, those showing significant differences in ECH or acteoside 488 production across different batches (artificially planted plant samples) or between the 489 treatment and control groups (callus) underwent transcriptome sequencing. .. Samples 490 stored at -80°C were used for total RNA extraction, mRNA sequencing library construction, 491 cDNA sequencing, and de novo assembly, with transcriptome sequencing completed by 492 the Beijing Genomics Institution. ..

Article Title: Deletion of Nrf1α exacerbates oxidative stress-induced cellular senescence by disrupting cell homeostasis.
Article Snippet: Cellular senescence is recognized as a fundamental hallmark contributing to ageing and various age-related diseases, with oxidative stress playing a critical initiating role in their pathological processes.. However, the anti-senescence potential of the antioxidant nuclear factor erythroid-derived 2-like 1 (Nrf1, encoded by Nfe2l1) remains elusive, despite accumulating evidence demonstrating its role as an indispensable redox-determining transcription factor for maintaining cellular homeostasis and organ integrity.. This study reveals that deletion of Nrf1α significantly elevates senescence characteristics in Nrf1α /− -deficient cells, as evidenced by two distinct experimental models.

Mutagenesis:

Article Title: Genome-Wide Identification and Characterization of the Class III Peroxidase Gene Family in Radish ( Raphanus sativus ) with Insights into Their Roles in Anthocyanin Metabolism.
Article Snippet: .. Transcriptome sequencing of the tissues of the chimeric-colored radish mutant was completed by BGI (Beijing Genomics Institute, Shenzhen, China). ..

Quantitative Proteomics:

Article Title: Schisandrin B targets CDK4/6 to suppress proliferation and enhance radiosensitivity in nasopharyngeal carcinoma by inducing cell cycle arrest.
Article Snippet: 250 μL PI/RNase (Roche, Shanghai, China) was added, following by incubation in dark at room temperature for 15 min. After screening, cell cycle distribution was analyzed by a BD flow cytometer (Franklin Lakes, NJ, USA). .. Transcriptome sequencing and differential expression analysis We prepared samples of HONE-1, HONE-1-SchB, NP69, and NP69-SchB to send to the Beijing Genomics Institute (BGI) for sequencing (Drug effects for 48 h), and sequencing was repeated three times. ..

RNA Extraction:

Article Title: The complete synthetic pathway of echinacoside from Cistanche deserticola and its de novo biosynthesis in yeast.
Article Snippet: The medium used was 1/2 MS 482 medium supplemented with 20 g/L sucrose, 500 mg/L amicase, and 0.6 g/L activated 483 charcoal, with pH adjusted to 5.6-6.0, and sterilized at 115°C for 20 min. 484 Transcriptome sequencing and analysis of sampled plants and callus of C. 485 deserticola 486 After high-performance liquid chromatography (HPLC) analysis of all samples from plant 487 samples and callus samples, those showing significant differences in ECH or acteoside 488 production across different batches (artificially planted plant samples) or between the 489 treatment and control groups (callus) underwent transcriptome sequencing. .. Samples 490 stored at -80°C were used for total RNA extraction, mRNA sequencing library construction, 491 cDNA sequencing, and de novo assembly, with transcriptome sequencing completed by 492 the Beijing Genomics Institution. ..

Article Title: Deletion of Nrf1α exacerbates oxidative stress-induced cellular senescence by disrupting cell homeostasis.
Article Snippet: Cellular senescence is recognized as a fundamental hallmark contributing to ageing and various age-related diseases, with oxidative stress playing a critical initiating role in their pathological processes.. However, the anti-senescence potential of the antioxidant nuclear factor erythroid-derived 2-like 1 (Nrf1, encoded by Nfe2l1) remains elusive, despite accumulating evidence demonstrating its role as an indispensable redox-determining transcription factor for maintaining cellular homeostasis and organ integrity.. This study reveals that deletion of Nrf1α significantly elevates senescence characteristics in Nrf1α /− -deficient cells, as evidenced by two distinct experimental models.

Isolation:

Article Title: Deletion of Nrf1α exacerbates oxidative stress-induced cellular senescence by disrupting cell homeostasis.
Article Snippet: Cellular senescence is recognized as a fundamental hallmark contributing to ageing and various age-related diseases, with oxidative stress playing a critical initiating role in their pathological processes.. However, the anti-senescence potential of the antioxidant nuclear factor erythroid-derived 2-like 1 (Nrf1, encoded by Nfe2l1) remains elusive, despite accumulating evidence demonstrating its role as an indispensable redox-determining transcription factor for maintaining cellular homeostasis and organ integrity.. This study reveals that deletion of Nrf1α significantly elevates senescence characteristics in Nrf1α /− -deficient cells, as evidenced by two distinct experimental models.

Purification:

Article Title: Deletion of Nrf1α exacerbates oxidative stress-induced cellular senescence by disrupting cell homeostasis.
Article Snippet: Cellular senescence is recognized as a fundamental hallmark contributing to ageing and various age-related diseases, with oxidative stress playing a critical initiating role in their pathological processes.. However, the anti-senescence potential of the antioxidant nuclear factor erythroid-derived 2-like 1 (Nrf1, encoded by Nfe2l1) remains elusive, despite accumulating evidence demonstrating its role as an indispensable redox-determining transcription factor for maintaining cellular homeostasis and organ integrity.. This study reveals that deletion of Nrf1α significantly elevates senescence characteristics in Nrf1α /− -deficient cells, as evidenced by two distinct experimental models.

other:

Article Title: CENPF overexpression in bladder cancer cells enhances proliferation, migration, invasion, and apoptosis.
Article Snippet: High-throughput whole transcriptome sequencing and data analysis were conducted in collaboration with the Beijing Genomics Institute (Shenzhen, China).



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