rgpx4 (OriGene)
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rgpx4
Rgpx4, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gpx4/Glutathione+Peroxidase+4+(GPX4)+(NM_002085)+Human+Recombinant+Protein/pmc10690572-33-0-2
Average 91 stars, based on 1 article reviews
Rgpx4, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gpx4/Glutathione+Peroxidase+4+(GPX4)+(NM_002085)+Human+Recombinant+Protein/pmc10690572-33-0-2
Average 91 stars, based on 1 article reviews
rgpx4 - by Bioz Stars,
2026-09
91/100 stars
Images
1) Product Images from "TXNDC12 inhibits lipid peroxidation and ferroptosis"
Article Title: TXNDC12 inhibits lipid peroxidation and ferroptosis
Journal: iScience
doi: 10.1016/j.isci.2023.108393
Figure Legend Snippet: TXNDC12 inhibits ferroptosis independent on GPX4 (A) The protein expression of GPX4 was analyzed by western blot in indicated K562 cells with or without GPX4 overexpression. (B) Cell death analysis was performed on the indicated K562 cells with or without GPX4 overexpression in response to erastin (10 μM) or RSL3 (0.5 μM) for 4–24 h. (C) The levels of MDA were analyzed in the indicated K562 cells with or without GPX4 overexpression in response to erastin (10 μM) or RSL3 (0.5 μM) for 24 h. (D) The protein expression of TXNDC12 was analyzed by western blot in indicated WT and Gpx4 -knockout Pfa-1 cells with or without Txndc12 overexpression. (E) Cell death analysis was performed on the indicated Pfa-1 cells with or without Txndc12 overexpression. (F) The levels of MDA were analyzed in the indicated Pfa-1 cells at 72 h. (G) Cell death analysis was performed on the indicated Pfa-1 cells in response to erastin (5 μM) for 24 h. The data represent the means ± SD from three independent samples. Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons test. ∗p < 0.05 versus WT group. (H) IP analysis was performed on K562 cells in response to erastin (10 μM) for 12 h. (I) Fluorescent calcein dye was entrapped within liposomes, which were subsequently exposed to Fe 2+ (10 μM) under conditions either with or without the presence of ferrostain-1 (1 μM), rGPX4 (200 nM), or rTXNDC1 (200 nM) for a duration of 20 min. The release of calcein from the liposomes was assessed by measuring changes in calcein fluorescence intensity. The data represent the means ± SD from three independent samples. Statistical analysis was performed using one-way ANOVA with Tukey’s multiple comparisons test. ∗p < 0.05 versus Fe group.
Techniques Used: Expressing, Western Blot, Over Expression, Knock-Out, Liposomes, Fluorescence
Figure Legend Snippet:
Techniques Used: Recombinant, Lysis, Bicinchoninic Acid Protein Assay, CCK-8 Assay, Transfection, Reporter Assay, Iron Assay, Multiple Displacement Amplification, Magnetic Beads, Mutagenesis, Chromatin Immunoprecipitation, Purification, Enzyme-linked Immunosorbent Assay, shRNA, Software
Related Articles
Liposomes:Article Title: TXNDC12 inhibits lipid peroxidation and ferroptosis Article Snippet: The free calcein was removed by gel filtration on a Sephadex G-50 column (GE Healthcare, 17004201), and the liposomes were collected and adjusted to a lipid concentration of 0.8 mg/mL before use. .. The liposomes were incubated with Fe 2+ (10 μM) under conditions either with or without the presence of ferrostain-1 (1 μM), Incubation:Article Title: TXNDC12 inhibits lipid peroxidation and ferroptosis Article Snippet: The free calcein was removed by gel filtration on a Sephadex G-50 column (GE Healthcare, 17004201), and the liposomes were collected and adjusted to a lipid concentration of 0.8 mg/mL before use. .. The liposomes were incubated with Fe 2+ (10 μM) under conditions either with or without the presence of ferrostain-1 (1 μM), |
