Review




Structured Review

Promega goscripttm qpcr master mix
MG-Exos protect endothelia cells against the oxidative effects by modulating the keap1/Nrf2/HO-1 signaling pathway. (a) Representative immunofluorescence images of the Nrf2's expression of bEnd.3 after Vehicle or MG-Exo treatment when exposed to H 2 O 2 -induced oxidative stress. Scale bar = 100 μ m. (b) Quantification of mean fluorescence intensity (MFI) in (a). (c) Western blotting analysis of the protein levels of keap1, Nrf2, and HO-1 in H 2 O 2 plus Vehicle or MG-Exo treatment groups. (d–g) <t>qPCR</t> verification of the mRNA levels of downstream antioxidative-related genes including NQo1 , Gclc , Cat , and Gsx1 per group when exposed to H 2 O 2 -induced oxidative stress. The data are presented as the means ± SD; n = 6 per group. ∗ p < 0.05 and ∗∗ p < 0.01 compared with different treatment groups.
Goscripttm Qpcr Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscripttm+rt+system/goscripttm+rt+master+mix/pmc08413072-104-31-35
Average 90 stars, based on 1 article reviews
goscripttm qpcr master mix - by Bioz Stars, 2026-11
90/100 stars

Images

1) Product Images from "Microglia-Derived Exosomes Improve Spinal Cord Functional Recovery after Injury via Inhibiting Oxidative Stress and Promoting the Survival and Function of Endothelia Cells"

Article Title: Microglia-Derived Exosomes Improve Spinal Cord Functional Recovery after Injury via Inhibiting Oxidative Stress and Promoting the Survival and Function of Endothelia Cells

Journal: Oxidative Medicine and Cellular Longevity

doi: 10.1155/2021/1695087

MG-Exos protect endothelia cells against the oxidative effects by modulating the keap1/Nrf2/HO-1 signaling pathway. (a) Representative immunofluorescence images of the Nrf2's expression of bEnd.3 after Vehicle or MG-Exo treatment when exposed to H 2 O 2 -induced oxidative stress. Scale bar = 100 μ m. (b) Quantification of mean fluorescence intensity (MFI) in (a). (c) Western blotting analysis of the protein levels of keap1, Nrf2, and HO-1 in H 2 O 2 plus Vehicle or MG-Exo treatment groups. (d–g) qPCR verification of the mRNA levels of downstream antioxidative-related genes including NQo1 , Gclc , Cat , and Gsx1 per group when exposed to H 2 O 2 -induced oxidative stress. The data are presented as the means ± SD; n = 6 per group. ∗ p < 0.05 and ∗∗ p < 0.01 compared with different treatment groups.
Figure Legend Snippet: MG-Exos protect endothelia cells against the oxidative effects by modulating the keap1/Nrf2/HO-1 signaling pathway. (a) Representative immunofluorescence images of the Nrf2's expression of bEnd.3 after Vehicle or MG-Exo treatment when exposed to H 2 O 2 -induced oxidative stress. Scale bar = 100 μ m. (b) Quantification of mean fluorescence intensity (MFI) in (a). (c) Western blotting analysis of the protein levels of keap1, Nrf2, and HO-1 in H 2 O 2 plus Vehicle or MG-Exo treatment groups. (d–g) qPCR verification of the mRNA levels of downstream antioxidative-related genes including NQo1 , Gclc , Cat , and Gsx1 per group when exposed to H 2 O 2 -induced oxidative stress. The data are presented as the means ± SD; n = 6 per group. ∗ p < 0.05 and ∗∗ p < 0.01 compared with different treatment groups.

Techniques Used: Immunofluorescence, Expressing, Fluorescence, Western Blot

Related Articles

Reverse Transcription:

Article Title: Downregulation of CRTC1 Is Involved in CUMS-Induced Depression-Like Behavior in the Hippocampus and Its RNA Sequencing Analysis.
Article Snippet: Chronic stress is an important risk factor for mood disorders including depression.. The decreased level of CREB (cAMPresponsive element binding)-regulated transcription coactivator 1 (CRTC1) expression in hippocampus may be involved in depression-like behavior in some stress-induced depression models.. But the mechanism of CRTC1 in mediating depressionlike behavior remains unknown.

Article Title: Fabrication of aptamer-guided siRNA loaded lipopolyplexes for gene silencing of notch 1 in MDA-mb-231 triple negative breast cancer cell line
Article Snippet: Triple negative breast cancer is an aggressive type of cancer with limited therapeutic options.. Aptamerfunctionalized liposomes to deliver short interference RNAs (siRNAs) offer a promising therapeutic option.. The current study aimed at preparing an aptamer functionalized liposome loaded with siRNA-protamine polyplexes for selective delivery of Notch 1 siRNA into cancer cells.

Article Title: Enhancing chemosensitivity of PANC1 pancreatic cancer cells to gemcitabine using ANGTPL4, Notch1 and NF-κβ1 siRNAs
Article Snippet: The extracted RNA was quantified by a Nanodrop (Thermo Fisher Scientific). .. In order to synthesize cDNA, cDNA synthesis is required for the next step, so 0.5 μg of total RNA was reverse transcribed by GoScriptTM RT Master Mix (Promega, USA) according to manufacturer protocol. .. The reverse transcription reaction was performed in a PCR instrument (T100TM Thermal cycler, BioRad).

Article Title: Enhancing chemosensitivity of wild-type and drug-resistant MDA-MB-231 triple-negative breast cancer cell line to doxorubicin by silencing of STAT 3, Notch-1, and β-catenin genes.
Article Snippet: Background/objective The absence of receptors in triple-negative breast cancer limits therapeutic choices utilized in clinical management of the disease.. Doxorubicin is an important member of therapeutic regimens that is hindered by emergence of resistance.. The current work aim to investigate of therapeutic potential of single and combinations of siRNA molecules designed for silencing STAT 3, Notch-1, and β-catenin genes in wild type and doxorubicin resistant MDA-MB-231 triple negative breast cancer cell line.

Article Title: Targeted delivery of CD163 + macrophage-derived small extracellular vesicles via RGD peptides promote vascular regeneration and stabilization after spinal cord injury.
Article Snippet: Targeted delivery of small extracellular vesicles (sEVs) with low immunogenicity and fewer undesirable side effects are needed for spinal cord injury (SCI) therapy.. Here, we show that RGD (Arg-Gly-Asp) peptide-decorated CD163 macrophage-derived sEVs can deliver TGF-β to the neovascular endothelial cells of the injured site and improve neurological function after SCI.. CD163 macrophages are M2 macrophages that express TGF-β and are reported to promote angiogenesis and vascular stabilization in various diseases.

Article Title: Nucleus‐localized circSLC39A5 suppresses hepatocellular carcinoma development by binding to STAT1 to regulate TDG transcription
Article Snippet: Total RNA was extracted from tissues or cells using the TRIzol reagent (Invitrogen), and RNA was extracted from plasma using the TRIzol LS reagent (Invitrogen). .. Then, complementary DNA (cDNA) was synthesized according to the instructions of the GoScriptTM Reverse Transcription System (Promega). qPCR was performed using GoScriptTM qPCR Master Mix (Promega) and QuantStudioTM7 (Q7) Flex Real‐Time PCR System. ..

Expressing:

Article Title: Enhancing chemosensitivity of wild-type and drug-resistant MDA-MB-231 triple-negative breast cancer cell line to doxorubicin by silencing of STAT 3, Notch-1, and β-catenin genes.
Article Snippet: Background/objective The absence of receptors in triple-negative breast cancer limits therapeutic choices utilized in clinical management of the disease.. Doxorubicin is an important member of therapeutic regimens that is hindered by emergence of resistance.. The current work aim to investigate of therapeutic potential of single and combinations of siRNA molecules designed for silencing STAT 3, Notch-1, and β-catenin genes in wild type and doxorubicin resistant MDA-MB-231 triple negative breast cancer cell line.

cDNA Synthesis:

Article Title: Fabrication of aptamer-guided siRNA loaded lipopolyplexes for gene silencing of notch 1 in MDA-mb-231 triple negative breast cancer cell line
Article Snippet: Triple negative breast cancer is an aggressive type of cancer with limited therapeutic options.. Aptamerfunctionalized liposomes to deliver short interference RNAs (siRNAs) offer a promising therapeutic option.. The current study aimed at preparing an aptamer functionalized liposome loaded with siRNA-protamine polyplexes for selective delivery of Notch 1 siRNA into cancer cells.

Article Title: Enhancing chemosensitivity of PANC1 pancreatic cancer cells to gemcitabine using ANGTPL4, Notch1 and NF-κβ1 siRNAs
Article Snippet: The extracted RNA was quantified by a Nanodrop (Thermo Fisher Scientific). .. In order to synthesize cDNA, cDNA synthesis is required for the next step, so 0.5 μg of total RNA was reverse transcribed by GoScriptTM RT Master Mix (Promega, USA) according to manufacturer protocol. .. The reverse transcription reaction was performed in a PCR instrument (T100TM Thermal cycler, BioRad).

other:


Quantitative RT-PCR:

Article Title: Targeted delivery of CD163 + macrophage-derived small extracellular vesicles via RGD peptides promote vascular regeneration and stabilization after spinal cord injury.
Article Snippet: Targeted delivery of small extracellular vesicles (sEVs) with low immunogenicity and fewer undesirable side effects are needed for spinal cord injury (SCI) therapy.. Here, we show that RGD (Arg-Gly-Asp) peptide-decorated CD163 macrophage-derived sEVs can deliver TGF-β to the neovascular endothelial cells of the injured site and improve neurological function after SCI.. CD163 macrophages are M2 macrophages that express TGF-β and are reported to promote angiogenesis and vascular stabilization in various diseases.

Real-time Polymerase Chain Reaction:

Article Title: Targeted delivery of CD163 + macrophage-derived small extracellular vesicles via RGD peptides promote vascular regeneration and stabilization after spinal cord injury.
Article Snippet: Targeted delivery of small extracellular vesicles (sEVs) with low immunogenicity and fewer undesirable side effects are needed for spinal cord injury (SCI) therapy.. Here, we show that RGD (Arg-Gly-Asp) peptide-decorated CD163 macrophage-derived sEVs can deliver TGF-β to the neovascular endothelial cells of the injured site and improve neurological function after SCI.. CD163 macrophages are M2 macrophages that express TGF-β and are reported to promote angiogenesis and vascular stabilization in various diseases.

Article Title: Nucleus‐localized circSLC39A5 suppresses hepatocellular carcinoma development by binding to STAT1 to regulate TDG transcription
Article Snippet: Total RNA was extracted from tissues or cells using the TRIzol reagent (Invitrogen), and RNA was extracted from plasma using the TRIzol LS reagent (Invitrogen). .. Then, complementary DNA (cDNA) was synthesized according to the instructions of the GoScriptTM Reverse Transcription System (Promega). qPCR was performed using GoScriptTM qPCR Master Mix (Promega) and QuantStudioTM7 (Q7) Flex Real‐Time PCR System. ..

Synthesized:

Article Title: Nucleus‐localized circSLC39A5 suppresses hepatocellular carcinoma development by binding to STAT1 to regulate TDG transcription
Article Snippet: Total RNA was extracted from tissues or cells using the TRIzol reagent (Invitrogen), and RNA was extracted from plasma using the TRIzol LS reagent (Invitrogen). .. Then, complementary DNA (cDNA) was synthesized according to the instructions of the GoScriptTM Reverse Transcription System (Promega). qPCR was performed using GoScriptTM qPCR Master Mix (Promega) and QuantStudioTM7 (Q7) Flex Real‐Time PCR System. ..



Similar Products

90
Promega goscripttm reverse transcription (rt) system
Goscripttm Reverse Transcription (Rt) System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscripttm+rt+system/goscripttm+reverse+transcription+system/10__1016_slash_j__vas__2025__100471-63-12-17
Average 90 stars, based on 1 article reviews
goscripttm reverse transcription (rt) system - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

90
Promega goscripttm reverse transcription system kit for rt-qpcr
Goscripttm Reverse Transcription System Kit For Rt Qpcr, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscripttm+rt+system/goscripttm+reverse+transcription+system+protocol/pm40280059-66-16-17
Average 90 stars, based on 1 article reviews
goscripttm reverse transcription system kit for rt-qpcr - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

90
Promega goscripttm rt system
Goscripttm Rt System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscripttm+rt+system/goscript+rt+system/10__3390_slash_nu17060966-79-14-17
Average 90 stars, based on 1 article reviews
goscripttm rt system - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

90
Promega goscripttm reverse transcription (rt-pcr) kit
Goscripttm Reverse Transcription (Rt Pcr) Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscripttm+rt+system/goscripttm+reverse+transcription+system/pmc11602827-71-1-9
Average 90 stars, based on 1 article reviews
goscripttm reverse transcription (rt-pcr) kit - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

90
Promega goscripttm 5x rt reaction buffer
Goscripttm 5x Rt Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscripttm+rt+system/goscript+5x+reaction+buffer/pmc11680345-65-10-15
Average 90 stars, based on 1 article reviews
goscripttm 5x rt reaction buffer - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

90
Promega goscripttm rt reagent kit (promega, madison, wi, usa)
Goscripttm Rt Reagent Kit (Promega, Madison, Wi, Usa), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscripttm+rt+system/goscripttm+rt+kit/pm39590363-102-22-26
Average 90 stars, based on 1 article reviews
goscripttm rt reagent kit (promega, madison, wi, usa) - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

90
Promega rt kit promega goscripttm reverse transcription system
Rt Kit Promega Goscripttm Reverse Transcription System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscripttm+rt+system/goscripttm+reverse+transcription+system/pm39021735-100-6-8
Average 90 stars, based on 1 article reviews
rt kit promega goscripttm reverse transcription system - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

90
Promega goscripttm rt mix for 1-step rt-qpcr
Goscripttm Rt Mix For 1 Step Rt Qpcr, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscripttm+rt+system/gotaq+qpcr+master+mix/pmc11312051-118-23-31
Average 90 stars, based on 1 article reviews
goscripttm rt mix for 1-step rt-qpcr - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

Image Search Results