goscripttm rt reagent kit (promega, madison, wi, usa) (Promega)

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Promega goscripttm rt reagent kit (promega, madison, wi, usa)
Goscripttm Rt Reagent Kit (Promega, Madison, Wi, Usa), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscripttm+rt+system/goscripttm+rt+kit/pm39590363-102-22-26
Average 90 stars, based on 1 article reviews

goscripttm rt reagent kit (promega, madison, wi, usa) - by Bioz Stars, 2026-11

90/100 stars

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Article Title: Radix Angelica Sinensis and Radix Hedysari Ultrafiltration Extract Protects against X-Irradiation-Induced Cardiac Fibrosis in Rats
Article Snippet: First-strand cDNA was synthesized from mRNA with a GoScript RT reagent kit (Promega, Beijing, China) according to the protocol provided by the manufacturer.

Reverse Transcription:

Article Title: Association of Circular RNAs levels in blood and Essential Hypertension with Carotid Plaque.
Article Snippet: The concentration and purity of RNA were determined by the MultiskanTM GO Microplate Reader (Thermo Fisher Scientific, Waltham, MA, USA). .. According to the manufacturer’s instructions, the reverse transcription (RT) mixture system was prepared using the GoScriptTM RT Reagent Kit (Promega, Madison, WI, USA). .. Then, the RT reaction was performed by the Mastercycler gradient PCR instrument (Eppendorf, Hamburg, DE).

Article Title: Identification and verification of circRNA biomarkers for coronary artery disease based on WGCNA and the LASSO algorithm
Article Snippet: The concentration and purity of total RNA were quantified by Multiskan GO Microplate Reader (ThermoFisher, Waltham, MA, USA). .. The GoScriptTM RT Reagent Kit (Promega, Madison, WI, USA) was utilized to prepare the reverse transcription mixture system. .. The expression levels of circRNAs were quantified by qRT-PCR using SYBR Green GoTaq Master Mix (Promega, Madison, WI, USA) on the LightCycler 480II Real-Time PCR System (Roche, Rotkreuz, Switzerland) in triplicate for each sample.

Article Title: Genome-wide characterization of tobacco B-BOX gene family identified two close members play contrast roles under cold stress
Article Snippet: Cold stress influences the growth and geographical distribution of plants.. Tobacco (Nicotiana tabacum L.), a model in plant biology studies and a world-widely cultivated economic plant from tropical America, exhibits extreme sensitivity to low temperatures.. The conserved B-BOX (BBX) transcription factors play vital roles in plant photomorphogenesis and flowering regulation, and their functions in abiotic stresses, including cold stress, have been uncovered recently.

Article Title: Transcriptome analysis to reveal the mechanism of the effect of Echinops latifolius polysaccharide B on palmitate-induced insulin-resistant.
Article Snippet: .. Reverse transcription was performed with GoScriptTM RT reagent Kit (Promega, USA), and the qPCR was performed using a GoTaq qPCR Master Mix (Promega, USA). ..

Article Title: miR-361 enhances sensitivity to 5-fluorouracil by targeting the FOXM1-ABCC5/10 signaling pathway in colorectal cancer
Article Snippet: Total RNA was isolated using TRIzol ® reagent (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. miRNA was obtained using an miRcute miRNA extraction kit (Tiangen Biotech Co. Ltd.). .. RNA and miRNA were reverse transcribed into cDNA using a GoScriptTM RT reagent kit (Promega Corporation) according to the manufacturer's protocol. qPCR analysis was subsequently performed using SYBR ® Green Premix Ex TaqTM II (Takara Biotechnology Co., Ltd.) and an ABI Prism 7900 Sequence Detection system (Applied Biosystems; Thermo Fisher Scientific, Inc.). .. The miR-361 mimic (sequence 5′-ACCCCUGGAGAUUCUGAUAAUU-3′), miR-361 mimic negative control (NC) (sequence 5′-UUCUCCGAACGUGUCACGUTT-3′), Forkhead box M1 (FOXM1) small interfering (si)RNA (siFOXM1 sequence: Sense: 5′-GGACCACUUUUCCCUACUUUDTDT-3′, antisense: 5′-AAAGUAGGGAAAGUGGUCCDTDT-3′) and siRNA NC (sequence 5′-UUCUCCGAACGUGUCACGUTT-3′, antisense: 5′-ACGUGACACGUUCGGAGAATT-3′) were designed and synthesized by Shanghai GenePharma Co., Ltd. A total of 2×10 5 HCT116-Res and HT29-Res cells were seeded in 6-well plates.

Real-time Polymerase Chain Reaction:

Extraction:

Article Title: Ultrafiltration Extract of Radix Angelica Sinensis and Radix Hedysari Attenuates Risk of Low-Dose X-Ray Radiation-Induced Myocardial Fibrosis In Vitro.
Article Snippet: .. According to indication of manufacture, extraction of Total cellular RNA by TRIZOL reagent (Ambion, Carlsbad, CA), Synthetism of First-strand cDNA was from mRNA with a Go ScriptTM RT reagent kit (Promega, Beijing, China). .. +e mRNA expression levels of TGF-β1, Col1, α-SMA, and GAPDH (internal control) were analyzed by real-time PCR with a Go Taq ® qPCR MasterMix kit (Promega, Beijing, China).

SYBR Green Assay:

Sequencing:

Isolation:

Article Title: Construction of Immune-Related circRNA-miRNA-mRNA Network and Identification of circRNAs as Biomarkers in Coronary Atherosclerotic Heart Disease.
Article Snippet: To establish a cell model of atherosclerosis, THP-1 macrophages were stimulated by 100 μg/mL ox-LDL (Yiyuan Biotechnologies, Guangzhou, China) for 48 h to form foam cells. .. Total RNAs were isolated from the blood samples and THP-1 macrophages with TRIzolTM kit (Bioteke, Beijing, China) and converted to cDNA using GoScriptTM RT Reagent Kit (Promega, Madison, WI, USA). .. The expression levels of the circRNAs were measured by qRT-PCR using the LightCycler 480II Real-Time PCR System (Roche, Rotkreuz, Switzerland).

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