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truseq stranded total rna library prep kit  (Illumina Inc)


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    Structured Review

    Illumina Inc truseq stranded total rna library prep kit
    Heatmap of the DEGs in neuroprogenitors ( A ) and cortical neurons ( B ) and validation by qPCR ( C ). ( A ) Heatmap showing the expressions (z-scores) of differentially expressed genes in neuroprogenitor cells between controls and cells with duplication (fourth independent differentiations). ( B ) Heatmap showing the expressions (z-scores) of differentially expressed genes in cortical neurons between controls and cells with duplication, a pink brain next to the gene name represents a neuronal/synapse related function and a blue brain next to the gene name represents genes associated with neurodevelopmental disorders. ( C ) qRT-PCR validation from randomly selected upregulated and downregulated genes; <t>total</t> <t>RNA</t> from genotypes at day 14 and day 63 was used to analyze expression levels, and data were normalized using TBP as the reference gene.
    Truseq Stranded Total Rna Library Prep Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 98/100, based on 6176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goscript+rt-+pcr+kit/TruSeq+Stranded+Total+RNA+Library+Prep+Gold/pmc11764630-79-19-34
    Average 98 stars, based on 6176 article reviews
    truseq stranded total rna library prep kit - by Bioz Stars, 2026-09
    98/100 stars

    Images

    1) Product Images from "Chromosome 4 Duplication Associated with Strabismus Leads to Gene Expression Changes in iPSC-Derived Cortical Neurons"

    Article Title: Chromosome 4 Duplication Associated with Strabismus Leads to Gene Expression Changes in iPSC-Derived Cortical Neurons

    Journal: Genes

    doi: 10.3390/genes16010080

    Heatmap of the DEGs in neuroprogenitors ( A ) and cortical neurons ( B ) and validation by qPCR ( C ). ( A ) Heatmap showing the expressions (z-scores) of differentially expressed genes in neuroprogenitor cells between controls and cells with duplication (fourth independent differentiations). ( B ) Heatmap showing the expressions (z-scores) of differentially expressed genes in cortical neurons between controls and cells with duplication, a pink brain next to the gene name represents a neuronal/synapse related function and a blue brain next to the gene name represents genes associated with neurodevelopmental disorders. ( C ) qRT-PCR validation from randomly selected upregulated and downregulated genes; total RNA from genotypes at day 14 and day 63 was used to analyze expression levels, and data were normalized using TBP as the reference gene.
    Figure Legend Snippet: Heatmap of the DEGs in neuroprogenitors ( A ) and cortical neurons ( B ) and validation by qPCR ( C ). ( A ) Heatmap showing the expressions (z-scores) of differentially expressed genes in neuroprogenitor cells between controls and cells with duplication (fourth independent differentiations). ( B ) Heatmap showing the expressions (z-scores) of differentially expressed genes in cortical neurons between controls and cells with duplication, a pink brain next to the gene name represents a neuronal/synapse related function and a blue brain next to the gene name represents genes associated with neurodevelopmental disorders. ( C ) qRT-PCR validation from randomly selected upregulated and downregulated genes; total RNA from genotypes at day 14 and day 63 was used to analyze expression levels, and data were normalized using TBP as the reference gene.

    Techniques Used: Biomarker Discovery, Quantitative RT-PCR, Expressing

    Related Articles

    Sequencing:

    Article Title: Dissociation Between Metabolic Improvements and Mitochondrial Transcriptional Changes Following Exercise Training in Women with Polycystic Ovary Syndrome.
    Article Snippet: Agilent 153 RNA 600 Nano kit and Bioanalyzer instrument (Agilent Technologies) were used to assess 154 the quality of the total RNA samples. .. Sequencing libraries were prepared from 500 ng of 155 RNA using the Illumina TruSeq Stranded Total RNA kit with the Ribo-Zero Gold protocol 156 (Illumina), as previously described (17, 32). .. Quantification of libraries was performed using 157 the Qubit dsDNA HS assay kit (Invitrogen) to ensure optimum cluster densities.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Integrating tumor and immune cell transcriptomics to predict immune checkpoint inhibitor primary resistance in metastatic melanoma
    Article Snippet: .. RNA from the 46 tumor samples of the Discovery FFPE bulk RNA-seq subcohort was extracted with the RNeasy FFPE kit following the manufacturer's instructions (Qiagen, Düsseldorf, Germany; Ref. 73504) and RNA-Seq libraries were prepared using the TruSeq Stranded Total RNA Gold kit (Illumina, Ref. 20020598) with IDT for Illumina TruSeq RNA UD Indexes (Illumina, Ref. 20020591), capturing both coding and noncoding RNA through double ribosomal RNA depletion. ..

    Article Title: Integrating tumor and immune cell transcriptomics to predict immune checkpoint inhibitor primary resistance in metastatic melanoma
    Article Snippet: .. RNA from the 46 tumor samples of the Discovery FFPE bulk RNA-seq subcohort was extracted with the RNeasy FFPE kit following the manufacturer's instructions (Qiagen, Düsseldorf, Germany; Ref. 73504) and RNA-Seq libraries were prepared using the TruSeq Stranded Total RNA Gold kit (Illumina, Ref. 20020598) with IDT for Illumina TruSeq RNA UD Indexes (Illumina, Ref. 20020591), capturing both coding and noncoding RNA through double ribosomal RNA depletion. ..

    RNA Sequencing:

    Article Title: Integrating tumor and immune cell transcriptomics to predict immune checkpoint inhibitor primary resistance in metastatic melanoma
    Article Snippet: .. RNA from the 46 tumor samples of the Discovery FFPE bulk RNA-seq subcohort was extracted with the RNeasy FFPE kit following the manufacturer's instructions (Qiagen, Düsseldorf, Germany; Ref. 73504) and RNA-Seq libraries were prepared using the TruSeq Stranded Total RNA Gold kit (Illumina, Ref. 20020598) with IDT for Illumina TruSeq RNA UD Indexes (Illumina, Ref. 20020591), capturing both coding and noncoding RNA through double ribosomal RNA depletion. ..

    Article Title: Transcriptomic insights into thermal manipulation and heat stress in skeletal muscles of broiler chickens.
    Article Snippet: .. RNA-seq libraries were generated using the TruSeq Stranded Total RNA Library Prep Gold Kit (Illumina, CA, USA), following the manufacturer’s protocol (TruSeq Stranded Total RNA Reference Guide, 1000000040499 v00). .. Bulk RNA-seq was performed by Macrogen Inc., Korea, using Illumina sequencer, generating 101 bp paired-end reads.

    Article Title: Integrating tumor and immune cell transcriptomics to predict immune checkpoint inhibitor primary resistance in metastatic melanoma
    Article Snippet: .. RNA from the 46 tumor samples of the Discovery FFPE bulk RNA-seq subcohort was extracted with the RNeasy FFPE kit following the manufacturer's instructions (Qiagen, Düsseldorf, Germany; Ref. 73504) and RNA-Seq libraries were prepared using the TruSeq Stranded Total RNA Gold kit (Illumina, Ref. 20020598) with IDT for Illumina TruSeq RNA UD Indexes (Illumina, Ref. 20020591), capturing both coding and noncoding RNA through double ribosomal RNA depletion. ..

    RNA sequencing:

    Article Title: Integrating tumor and immune cell transcriptomics to predict immune checkpoint inhibitor primary resistance in metastatic melanoma
    Article Snippet: .. RNA from the 46 tumor samples of the Discovery FFPE bulk RNA-seq subcohort was extracted with the RNeasy FFPE kit following the manufacturer's instructions (Qiagen, Düsseldorf, Germany; Ref. 73504) and RNA-Seq libraries were prepared using the TruSeq Stranded Total RNA Gold kit (Illumina, Ref. 20020598) with IDT for Illumina TruSeq RNA UD Indexes (Illumina, Ref. 20020591), capturing both coding and noncoding RNA through double ribosomal RNA depletion. ..

    Article Title: Integrating tumor and immune cell transcriptomics to predict immune checkpoint inhibitor primary resistance in metastatic melanoma
    Article Snippet: .. RNA from the 46 tumor samples of the Discovery FFPE bulk RNA-seq subcohort was extracted with the RNeasy FFPE kit following the manufacturer's instructions (Qiagen, Düsseldorf, Germany; Ref. 73504) and RNA-Seq libraries were prepared using the TruSeq Stranded Total RNA Gold kit (Illumina, Ref. 20020598) with IDT for Illumina TruSeq RNA UD Indexes (Illumina, Ref. 20020591), capturing both coding and noncoding RNA through double ribosomal RNA depletion. ..

    Generated:

    Article Title: Transcriptomic insights into thermal manipulation and heat stress in skeletal muscles of broiler chickens.
    Article Snippet: .. RNA-seq libraries were generated using the TruSeq Stranded Total RNA Library Prep Gold Kit (Illumina, CA, USA), following the manufacturer’s protocol (TruSeq Stranded Total RNA Reference Guide, 1000000040499 v00). .. Bulk RNA-seq was performed by Macrogen Inc., Korea, using Illumina sequencer, generating 101 bp paired-end reads.

    Construct:

    Article Title: IL-17RA signaling promotes the dedifferentiation of Paneth progenitors through ADAM17 to regenerate gut epithelium post-irradiation.
    Article Snippet: .. Illumina-compatible cDNA libraries with RiboZero Gold depletion were constructed according to the TruSeq Stranded Total RNA Reference Guide (Illumina Document #1000000040499v00, TruSeq Stranded Total RNA Gold Illumina Kit #20020598). .. Final cDNA library concentrations were determined using the Qubit dsDNA BR assay kit (Thermo Fisher Scientific: Guide MAN0002325 MP 32850, Kit #Q32850).



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    a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) <t>PCR</t> demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , <t>RT-PCR</t> of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.
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    a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) <t>PCR</t> demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , <t>RT-PCR</t> of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.
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    Image Search Results


    a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) PCR demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , RT-PCR of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.

    Journal: Nature microbiology

    Article Title: A metabolic pathway for catabolizing levulinic acid in bacteria

    doi: 10.1038/s41564-017-0028-z

    Figure Lengend Snippet: a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) PCR demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , RT-PCR of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.

    Article Snippet: The Promega GoScript RT PCR kit was used to generate cDNA using 1 μL of a 10 μM gene specific oligo (JMR2 for lvaR and JMR287 for lvaA ) instead of the random oligo mixture.

    Techniques: Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Negative Control, Fluorescence, Clone Assay, Plasmid Preparation