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Promega rt/pcr kit goscript
Rt/Pcr Kit Goscript, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscript+rt-+pcr+kit/goscript+kit/pmc06350585-79-11-14
Average 90 stars, based on 1 article reviews
rt/pcr kit goscript - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Produced:

Article Title: The Stylo Cysteine-Rich Peptide SgSnakin1 Is Involved in Aluminum Tolerance through Enhancing Reactive Oxygen Species Scavenging
Article Snippet: For qRT-PCR analysis, the RNA of plant tissue was extracted using an RNA-solve reagent (OMEGA Bio-Tek, Norcross, GA, USA) following the manufacturer’s instructions. .. The complementary DNA was produced by 2 μg RNA through reverse transcription according to the GoScript kit (Promega, Madison, WI, USA). qRT-PCR analysis was performed using the SYBR Green PCR master mix kit and ABI7500 real-time PCR system (Thermo Fisher Scientific, Waltham, MA, USA). ..

Reverse Transcription:

Article Title: The Stylo Cysteine-Rich Peptide SgSnakin1 Is Involved in Aluminum Tolerance through Enhancing Reactive Oxygen Species Scavenging
Article Snippet: For qRT-PCR analysis, the RNA of plant tissue was extracted using an RNA-solve reagent (OMEGA Bio-Tek, Norcross, GA, USA) following the manufacturer’s instructions. .. The complementary DNA was produced by 2 μg RNA through reverse transcription according to the GoScript kit (Promega, Madison, WI, USA). qRT-PCR analysis was performed using the SYBR Green PCR master mix kit and ABI7500 real-time PCR system (Thermo Fisher Scientific, Waltham, MA, USA). ..

Article Title: Sanghuangporus vaninii extract ameliorates hyperlipidemia in rats by mechanisms identified with transcriptome analysis
Article Snippet: .. For real‐time quantitative evaluation of gene expression, RNA was extracted from liver samples with the SV Total RNA Isolation System (Promega, USA), and reverse transcribed using the GoScriptTM kit (Promega). .. Subsequently, the products were amplified using GoTaq® qPCR (Promega) in a quantitative PCR (qPCR) system (LineGene 9620, Hangzhou Bioer Technology Co., Ltd, China).

Article Title: <scp>TAD</scp>‐dependent sub‐<scp>TAD</scp> is required for enhancer–promoter interaction enabling the β‐globin transcription
Article Snippet: .. Total RNA was extracted from MEL/ch11 (2 × 106) cells using QIAzol (Qiagen) and reverse- transcribed into cDNA using a GoScript kit (Promega). ..

Article Title: Colony-stimulating factor 2 (CSF2) does not significantly affect cellular and molecular parameters, or blastocyst rates under in vitro culture conditions with reduced nutrient concentrations.
Article Snippet: Colony-stimulating factor 2 (CSF2) is an embryokine used between days 5 and 7 of culture to improve embryonic development.. We evaluated its use from Day 4 and throughout the culture period in a reduced nutrient concentrations culture medium.. Presumptive zygotes were assigned to three treatments: Control – synthetic oviduct fluid (SOF), CSF2 D1 (SOF + CSF2 from Day 1–7), CSF2 D4 (SOF + CSF2 from Day 4–7).

Article Title: Magnesium lithospermate B enhances the potential of human-induced pluripotent stem cell-derived cardiomyocytes for myocardial repair
Article Snippet: .. After isolating cellular total RNA, cDNA was generated by reverse transcription utilizing a GoScript kit (Promega, Madison, Wisconsin USA), as instructed by the manufacturer. .. After being collected, cDNA was employed as a template for qPCR, which was conducted with SYBR green on an ABI Prism 7900 Sequence Detection System as directed by the manufacturer.

Article Title: KH-like Domains in PARP9/DTX3L and PARP14 Coordinate Protein–Protein Interactions to Promote Cancer Cell Survival
Article Snippet: Total RNA was isolated using the Qiagen RNeasy RNA extraction kit (Qiagen, UK) following the manufacturer's instructions. .. Subsequently, 1 μg of the total RNA was subjected to reverse transcription using the GoScript kit (Promega, US) as per the supplier's protocol. ..

Quantitative RT-PCR:

Article Title: The Stylo Cysteine-Rich Peptide SgSnakin1 Is Involved in Aluminum Tolerance through Enhancing Reactive Oxygen Species Scavenging
Article Snippet: For qRT-PCR analysis, the RNA of plant tissue was extracted using an RNA-solve reagent (OMEGA Bio-Tek, Norcross, GA, USA) following the manufacturer’s instructions. .. The complementary DNA was produced by 2 μg RNA through reverse transcription according to the GoScript kit (Promega, Madison, WI, USA). qRT-PCR analysis was performed using the SYBR Green PCR master mix kit and ABI7500 real-time PCR system (Thermo Fisher Scientific, Waltham, MA, USA). ..

SYBR Green Assay:

Article Title: The Stylo Cysteine-Rich Peptide SgSnakin1 Is Involved in Aluminum Tolerance through Enhancing Reactive Oxygen Species Scavenging
Article Snippet: For qRT-PCR analysis, the RNA of plant tissue was extracted using an RNA-solve reagent (OMEGA Bio-Tek, Norcross, GA, USA) following the manufacturer’s instructions. .. The complementary DNA was produced by 2 μg RNA through reverse transcription according to the GoScript kit (Promega, Madison, WI, USA). qRT-PCR analysis was performed using the SYBR Green PCR master mix kit and ABI7500 real-time PCR system (Thermo Fisher Scientific, Waltham, MA, USA). ..

Polymerase Chain Reaction:

Article Title: The Stylo Cysteine-Rich Peptide SgSnakin1 Is Involved in Aluminum Tolerance through Enhancing Reactive Oxygen Species Scavenging
Article Snippet: For qRT-PCR analysis, the RNA of plant tissue was extracted using an RNA-solve reagent (OMEGA Bio-Tek, Norcross, GA, USA) following the manufacturer’s instructions. .. The complementary DNA was produced by 2 μg RNA through reverse transcription according to the GoScript kit (Promega, Madison, WI, USA). qRT-PCR analysis was performed using the SYBR Green PCR master mix kit and ABI7500 real-time PCR system (Thermo Fisher Scientific, Waltham, MA, USA). ..

Real-time Polymerase Chain Reaction:

Article Title: The Stylo Cysteine-Rich Peptide SgSnakin1 Is Involved in Aluminum Tolerance through Enhancing Reactive Oxygen Species Scavenging
Article Snippet: For qRT-PCR analysis, the RNA of plant tissue was extracted using an RNA-solve reagent (OMEGA Bio-Tek, Norcross, GA, USA) following the manufacturer’s instructions. .. The complementary DNA was produced by 2 μg RNA through reverse transcription according to the GoScript kit (Promega, Madison, WI, USA). qRT-PCR analysis was performed using the SYBR Green PCR master mix kit and ABI7500 real-time PCR system (Thermo Fisher Scientific, Waltham, MA, USA). ..

Gene Expression:

Article Title: Sanghuangporus vaninii extract ameliorates hyperlipidemia in rats by mechanisms identified with transcriptome analysis
Article Snippet: .. For real‐time quantitative evaluation of gene expression, RNA was extracted from liver samples with the SV Total RNA Isolation System (Promega, USA), and reverse transcribed using the GoScriptTM kit (Promega). .. Subsequently, the products were amplified using GoTaq® qPCR (Promega) in a quantitative PCR (qPCR) system (LineGene 9620, Hangzhou Bioer Technology Co., Ltd, China).

Isolation:

Article Title: Sanghuangporus vaninii extract ameliorates hyperlipidemia in rats by mechanisms identified with transcriptome analysis
Article Snippet: .. For real‐time quantitative evaluation of gene expression, RNA was extracted from liver samples with the SV Total RNA Isolation System (Promega, USA), and reverse transcribed using the GoScriptTM kit (Promega). .. Subsequently, the products were amplified using GoTaq® qPCR (Promega) in a quantitative PCR (qPCR) system (LineGene 9620, Hangzhou Bioer Technology Co., Ltd, China).

Article Title: Colony-stimulating factor 2 (CSF2) does not significantly affect cellular and molecular parameters, or blastocyst rates under in vitro culture conditions with reduced nutrient concentrations.
Article Snippet: Colony-stimulating factor 2 (CSF2) is an embryokine used between days 5 and 7 of culture to improve embryonic development.. We evaluated its use from Day 4 and throughout the culture period in a reduced nutrient concentrations culture medium.. Presumptive zygotes were assigned to three treatments: Control – synthetic oviduct fluid (SOF), CSF2 D1 (SOF + CSF2 from Day 1–7), CSF2 D4 (SOF + CSF2 from Day 4–7).

Synthesized:

Article Title: Genomic and Phenotypic Variations Among Thai-53 and Mycobacterium leprae Clinical Isolates: Implications for Leprosy Pathogenesis and Research
Article Snippet: After centrifugation at 13,800× g at 15 min, DNA was precipitated from the aqueous phase after adding 300 μL of isopropanol (Sigma, St. Louis, MO, USA) and incubated overnight at −20 °C. .. DNA contaminants in the RNA samples were removed using the TURBO DNA-free kit (Ambion, Austin, TX, USA) and complementary DNA (cDNA) were synthesized using the GoScript kit (Promega, Madison, WI, USA) according to manufacturer’s instructions. ..

cDNA Synthesis:

Article Title: Colony-stimulating factor 2 (CSF2) does not significantly affect cellular and molecular parameters, or blastocyst rates under in vitro culture conditions with reduced nutrient concentrations.
Article Snippet: Colony-stimulating factor 2 (CSF2) is an embryokine used between days 5 and 7 of culture to improve embryonic development.. We evaluated its use from Day 4 and throughout the culture period in a reduced nutrient concentrations culture medium.. Presumptive zygotes were assigned to three treatments: Control – synthetic oviduct fluid (SOF), CSF2 D1 (SOF + CSF2 from Day 1–7), CSF2 D4 (SOF + CSF2 from Day 4–7).

Recombinant:

Article Title: Colony-stimulating factor 2 (CSF2) does not significantly affect cellular and molecular parameters, or blastocyst rates under in vitro culture conditions with reduced nutrient concentrations.
Article Snippet: Colony-stimulating factor 2 (CSF2) is an embryokine used between days 5 and 7 of culture to improve embryonic development.. We evaluated its use from Day 4 and throughout the culture period in a reduced nutrient concentrations culture medium.. Presumptive zygotes were assigned to three treatments: Control – synthetic oviduct fluid (SOF), CSF2 D1 (SOF + CSF2 from Day 1–7), CSF2 D4 (SOF + CSF2 from Day 4–7).

Generated:

Article Title: Magnesium lithospermate B enhances the potential of human-induced pluripotent stem cell-derived cardiomyocytes for myocardial repair
Article Snippet: .. After isolating cellular total RNA, cDNA was generated by reverse transcription utilizing a GoScript kit (Promega, Madison, Wisconsin USA), as instructed by the manufacturer. .. After being collected, cDNA was employed as a template for qPCR, which was conducted with SYBR green on an ABI Prism 7900 Sequence Detection System as directed by the manufacturer.



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a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) <t>PCR</t> demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , <t>RT-PCR</t> of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.
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a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) <t>PCR</t> demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , <t>RT-PCR</t> of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.
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a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) PCR demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , RT-PCR of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.

Journal: Nature microbiology

Article Title: A metabolic pathway for catabolizing levulinic acid in bacteria

doi: 10.1038/s41564-017-0028-z

Figure Lengend Snippet: a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) PCR demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , RT-PCR of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.

Article Snippet: The Promega GoScript RT PCR kit was used to generate cDNA using 1 μL of a 10 μM gene specific oligo (JMR2 for lvaR and JMR287 for lvaA ) instead of the random oligo mixture.

Techniques: Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Negative Control, Fluorescence, Clone Assay, Plasmid Preparation