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taqman gene expression master mix  (Thermo Fisher)


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    Thermo Fisher taqman gene expression master mix
    Taqman Gene Expression Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9894 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goscript+rt-+pcr+kit/TaqMa+Gene+Expression+Cells-to-CT+Kit/pmc05880723-116-34-39
    Average 99 stars, based on 9894 article reviews
    taqman gene expression master mix - by Bioz Stars, 2026-11
    99/100 stars

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    other:

    Article Title: Cellular iron governs the host response to malaria
    Article Snippet: Gene expression analysis by quantitative real-time PCR, was performed on liver samples preserved in RNAlater Stabilization Solution and stored at −80° C (AM7020, Thermo Fisher Scientific).

    Article Title: Immune-interacting lymphatic endothelial subtype at capillary terminals drives lymphatic malformation
    Article Snippet: Total RNA was extracted from FACS-sorted BECs and LECs, or CD45 + CD11b + Ly6G − F4/80 + myeloid cells, macrophages (Cd11b + MerTK + CD64 + ) and dendritic cells (MerTK − CD64 − CD11c + MHCII + ), using RNeasy Micro Kit (QIAGEN), according to the manufacturer’s instructions with additional DNaseI treatment (RNAse-Free DNase Set, QIAGEN).

    Article Title: Predictive biomarkers for the responsiveness of recurrent glioblastomas to activated killer cell immunotherapy
    Article Snippet: RT-qPCR quantitation was performed using TaqMan TM Gene expression assays [FAM] (Applied Biosystems, Carlsbad, CA, USA) for TNFSF4 (Hs01911853_s1), TNFRSF18 (Hs00188346_m1), and IL12RB2 (Hs00155486_m1) and TaqMan TM Gene expression Master Mix (Applied Biosystems, Foster City, CA, USA) on a Bio-Rad CFX96 Real-Time PCR Detection System (Bio-Rad).

    Polymerase Chain Reaction:

    Article Title: LDLR-mediated targeting and productive uptake of siRNA-peptide ligand conjugates in vitro and in vivo
    Article Snippet: The real-time quantitative PCR (qPCR) assays were performed using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad). .. Amplifications were carried out in a 10 μL final reaction solution containing 12.5ng of cDNA, 1X of TaqManTM Fast Universal PCR Master Mix (2X), no AmpEraseTM UNG (Invitrogen), 1X of TaqMan ® Gene Expression Assay Mix (Life Technologies) and RNase free water, according to the manufacturer’s recommendations. .. The following primers were used: Mm01344233_g1 SOD1(mouse), Mm02526700_g1 RpL13 (mouse), Mm01352366_m1 SDHA (mouse), Mm00457191_m1 PSMC4 (mouse).

    Gene Expression:

    Article Title: LDLR-mediated targeting and productive uptake of siRNA-peptide ligand conjugates in vitro and in vivo
    Article Snippet: The real-time quantitative PCR (qPCR) assays were performed using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad). .. Amplifications were carried out in a 10 μL final reaction solution containing 12.5ng of cDNA, 1X of TaqManTM Fast Universal PCR Master Mix (2X), no AmpEraseTM UNG (Invitrogen), 1X of TaqMan ® Gene Expression Assay Mix (Life Technologies) and RNase free water, according to the manufacturer’s recommendations. .. The following primers were used: Mm01344233_g1 SOD1(mouse), Mm02526700_g1 RpL13 (mouse), Mm01352366_m1 SDHA (mouse), Mm00457191_m1 PSMC4 (mouse).

    Article Title: Nuclease-dead S. aureus Cas9 downregulates alpha-synuclein and reduces mtDNA damage and oxidative stress levels in patient-derived stem cell model of Parkinson’s disease
    Article Snippet: .. TaqMan® Gene Expression Master Mix (ThermoFisher, Cat. No. 4369016) and TaqMan probes for FAM- SNCA (ThermoFisher, Assay ID: Hs00240906_m1) and VIC- GAPDH (ThermoFisher, Assay ID: Hs99999905_m1) were used to amplify cDNA. qPCR reactions were run in CFX96 Real Time System thermal cycler (Bio Rad) and Cq values were obtained using built-in software. ..

    Article Title: LSD1/PRMT6-targeting gene therapy to attenuate androgen receptor toxic gain-of-function ameliorates spinobulbar muscular atrophy phenotypes in flies and mice
    Article Snippet: The complete list of primer sequences (Eurofins) is provided in Supplementary Table . .. For RT-PCR in iPSC-derived motor neurons, 500 ng of RNA was reverse transcribed with the cDNA Reverse Transcription kit (Invitrogen, #4368814) and qPCR was performed in triplicate wells per sample using the Taqman Gene Expression Master Mix (Thermo Fisher Scientific, #4369510) on the QuantStudio 6 Flex machine (Thermo Fisher Scientific). .. Cells were then incubated at room temperature for 2 h with the following primary antibodies: AR (H280, Santa Cruz, #sc-13062, 1:500), PRMT6 (Santa Cruz, #sc-55702, 1:500), and LSD1 (Abcam, #ab17721, 1:500).

    Article Title: The phosphodiesterase 2A regulates lymphatic endothelial development via cGMP-mediated control of Notch signaling
    Article Snippet: RNA was reverse transcribed using SuperScript® IV VILO cDNA Synthesis Kit (Cat#: 11754050, Thermo Fisher Scientific) according to the manufacturer’s instructions. cDNA from sorted murine ECs was additionally pre-amplified using the TaqMan PreAmp Master Mix (Cat#: 4391128, Applied Biosystems). .. Gene expression levels were analyzed using TaqMan Gene Expression Assay (Cat#: 4369016, Applied Biosystems) and a StepOnePlus RT PCR system (Applied Biosystems). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Nuclease-dead S. aureus Cas9 downregulates alpha-synuclein and reduces mtDNA damage and oxidative stress levels in patient-derived stem cell model of Parkinson’s disease
    Article Snippet: .. TaqMan® Gene Expression Master Mix (ThermoFisher, Cat. No. 4369016) and TaqMan probes for FAM- SNCA (ThermoFisher, Assay ID: Hs00240906_m1) and VIC- GAPDH (ThermoFisher, Assay ID: Hs99999905_m1) were used to amplify cDNA. qPCR reactions were run in CFX96 Real Time System thermal cycler (Bio Rad) and Cq values were obtained using built-in software. ..

    Article Title: LSD1/PRMT6-targeting gene therapy to attenuate androgen receptor toxic gain-of-function ameliorates spinobulbar muscular atrophy phenotypes in flies and mice
    Article Snippet: The complete list of primer sequences (Eurofins) is provided in Supplementary Table . .. For RT-PCR in iPSC-derived motor neurons, 500 ng of RNA was reverse transcribed with the cDNA Reverse Transcription kit (Invitrogen, #4368814) and qPCR was performed in triplicate wells per sample using the Taqman Gene Expression Master Mix (Thermo Fisher Scientific, #4369510) on the QuantStudio 6 Flex machine (Thermo Fisher Scientific). .. Cells were then incubated at room temperature for 2 h with the following primary antibodies: AR (H280, Santa Cruz, #sc-13062, 1:500), PRMT6 (Santa Cruz, #sc-55702, 1:500), and LSD1 (Abcam, #ab17721, 1:500).

    Software:

    Article Title: Nuclease-dead S. aureus Cas9 downregulates alpha-synuclein and reduces mtDNA damage and oxidative stress levels in patient-derived stem cell model of Parkinson’s disease
    Article Snippet: .. TaqMan® Gene Expression Master Mix (ThermoFisher, Cat. No. 4369016) and TaqMan probes for FAM- SNCA (ThermoFisher, Assay ID: Hs00240906_m1) and VIC- GAPDH (ThermoFisher, Assay ID: Hs99999905_m1) were used to amplify cDNA. qPCR reactions were run in CFX96 Real Time System thermal cycler (Bio Rad) and Cq values were obtained using built-in software. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: LSD1/PRMT6-targeting gene therapy to attenuate androgen receptor toxic gain-of-function ameliorates spinobulbar muscular atrophy phenotypes in flies and mice
    Article Snippet: The complete list of primer sequences (Eurofins) is provided in Supplementary Table . .. For RT-PCR in iPSC-derived motor neurons, 500 ng of RNA was reverse transcribed with the cDNA Reverse Transcription kit (Invitrogen, #4368814) and qPCR was performed in triplicate wells per sample using the Taqman Gene Expression Master Mix (Thermo Fisher Scientific, #4369510) on the QuantStudio 6 Flex machine (Thermo Fisher Scientific). .. Cells were then incubated at room temperature for 2 h with the following primary antibodies: AR (H280, Santa Cruz, #sc-13062, 1:500), PRMT6 (Santa Cruz, #sc-55702, 1:500), and LSD1 (Abcam, #ab17721, 1:500).

    Article Title: The phosphodiesterase 2A regulates lymphatic endothelial development via cGMP-mediated control of Notch signaling
    Article Snippet: RNA was reverse transcribed using SuperScript® IV VILO cDNA Synthesis Kit (Cat#: 11754050, Thermo Fisher Scientific) according to the manufacturer’s instructions. cDNA from sorted murine ECs was additionally pre-amplified using the TaqMan PreAmp Master Mix (Cat#: 4391128, Applied Biosystems). .. Gene expression levels were analyzed using TaqMan Gene Expression Assay (Cat#: 4369016, Applied Biosystems) and a StepOnePlus RT PCR system (Applied Biosystems). ..

    Reverse Transcription:

    Article Title: LSD1/PRMT6-targeting gene therapy to attenuate androgen receptor toxic gain-of-function ameliorates spinobulbar muscular atrophy phenotypes in flies and mice
    Article Snippet: The complete list of primer sequences (Eurofins) is provided in Supplementary Table . .. For RT-PCR in iPSC-derived motor neurons, 500 ng of RNA was reverse transcribed with the cDNA Reverse Transcription kit (Invitrogen, #4368814) and qPCR was performed in triplicate wells per sample using the Taqman Gene Expression Master Mix (Thermo Fisher Scientific, #4369510) on the QuantStudio 6 Flex machine (Thermo Fisher Scientific). .. Cells were then incubated at room temperature for 2 h with the following primary antibodies: AR (H280, Santa Cruz, #sc-13062, 1:500), PRMT6 (Santa Cruz, #sc-55702, 1:500), and LSD1 (Abcam, #ab17721, 1:500).

    Construct:

    Article Title: Identification of mosquito proteins that differentially interact with alphavirus nonstructural protein 3, a determinant of vector specificity
    Article Snippet: Prior to shearing using the IONXpress Plus Fragment Library Kit, gDNA quantity was determined using a Qubit 2.0 Fluorometer (ThermoFisher Scientific) to optimize fragment lengths. .. Constructed libraries were barcoded using the Ion Xpress Barcode Adapter Kit (Life Technologies) to allow for multiplexed analysis on the Ion Torrent PGM. .. To assess the base-pair size profile as well as the quantity of amplified library, the sheared and purified cDNA library was analyzed on a Bioanalyzer (Agilent Technologies).



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    a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) <t>PCR</t> demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , <t>RT-PCR</t> of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.
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    a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) <t>PCR</t> demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , <t>RT-PCR</t> of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.
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    a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) PCR demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , RT-PCR of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.

    Journal: Nature microbiology

    Article Title: A metabolic pathway for catabolizing levulinic acid in bacteria

    doi: 10.1038/s41564-017-0028-z

    Figure Lengend Snippet: a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) PCR demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , RT-PCR of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.

    Article Snippet: The Promega GoScript RT PCR kit was used to generate cDNA using 1 μL of a 10 μM gene specific oligo (JMR2 for lvaR and JMR287 for lvaA ) instead of the random oligo mixture.

    Techniques: Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Negative Control, Fluorescence, Clone Assay, Plasmid Preparation