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truseq small rna library preparation kit  (Illumina Inc)


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    Structured Review

    Illumina Inc truseq small rna library preparation kit
    Figure 1—Hepatic miR-29 levels are upregulated in diet-induced insulin resistance in mice. A: Fasting blood glucose levels of C57BL/6J female mice on HFD for 16 weeks (n = 3) and matched LFD for 16 weeks (n = 3) are shown. B: miRNA expression levels (RPMM, reads per million mapped reads) based on deep sequenc- ing analysis of <t>small</t> <t>RNAs</t> from the livers of HFD-fed (n = 2) and LFD-fed (n = 2) C57BL/6J female mice are shown. Each circle rep- resents a miRNA that is expressed at RPMM >100 in at least one murine liver sample. Dashed blue lines represent 1.5-fold difference in expression between HFD-fed and LFD-fed mice. C: Relative lev- els (based on sequencing) of miR-29a, miR-29b, and miR-29c in the livers of HFD-fed (n = 2) and LFD-fed (n = 2) C57BL/6J female mice are shown. D: Relative levels (based on RT-qPCR) of miR-29a, miR- 29b, and miR-29c in the livers of HFD-fed (n = 3) and LFD-fed (n = 3) C57BL/6J female mice are shown. P values were calculated according to the one-tailed unpaired Student t test. a.u., arbitrary unit. *P < 0.05; **P < 0.01; ***P < 0.001.
    Truseq Small Rna Library Preparation Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 20641 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goscript+rt-+pcr+kit/TruSeq+RNA+Library+Preparation+Kit+v2/pm24722248-58-18-17
    Average 99 stars, based on 20641 article reviews
    truseq small rna library preparation kit - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "MicroRNA-29 fine-tunes the expression of key FOXA2-activated lipid metabolism genes and is dysregulated in animal models of insulin resistance and diabetes."

    Article Title: MicroRNA-29 fine-tunes the expression of key FOXA2-activated lipid metabolism genes and is dysregulated in animal models of insulin resistance and diabetes.

    Journal: Diabetes

    doi: 10.2337/db13-1015

    Figure 1—Hepatic miR-29 levels are upregulated in diet-induced insulin resistance in mice. A: Fasting blood glucose levels of C57BL/6J female mice on HFD for 16 weeks (n = 3) and matched LFD for 16 weeks (n = 3) are shown. B: miRNA expression levels (RPMM, reads per million mapped reads) based on deep sequenc- ing analysis of small RNAs from the livers of HFD-fed (n = 2) and LFD-fed (n = 2) C57BL/6J female mice are shown. Each circle rep- resents a miRNA that is expressed at RPMM >100 in at least one murine liver sample. Dashed blue lines represent 1.5-fold difference in expression between HFD-fed and LFD-fed mice. C: Relative lev- els (based on sequencing) of miR-29a, miR-29b, and miR-29c in the livers of HFD-fed (n = 2) and LFD-fed (n = 2) C57BL/6J female mice are shown. D: Relative levels (based on RT-qPCR) of miR-29a, miR- 29b, and miR-29c in the livers of HFD-fed (n = 3) and LFD-fed (n = 3) C57BL/6J female mice are shown. P values were calculated according to the one-tailed unpaired Student t test. a.u., arbitrary unit. *P < 0.05; **P < 0.01; ***P < 0.001.
    Figure Legend Snippet: Figure 1—Hepatic miR-29 levels are upregulated in diet-induced insulin resistance in mice. A: Fasting blood glucose levels of C57BL/6J female mice on HFD for 16 weeks (n = 3) and matched LFD for 16 weeks (n = 3) are shown. B: miRNA expression levels (RPMM, reads per million mapped reads) based on deep sequenc- ing analysis of small RNAs from the livers of HFD-fed (n = 2) and LFD-fed (n = 2) C57BL/6J female mice are shown. Each circle rep- resents a miRNA that is expressed at RPMM >100 in at least one murine liver sample. Dashed blue lines represent 1.5-fold difference in expression between HFD-fed and LFD-fed mice. C: Relative lev- els (based on sequencing) of miR-29a, miR-29b, and miR-29c in the livers of HFD-fed (n = 2) and LFD-fed (n = 2) C57BL/6J female mice are shown. D: Relative levels (based on RT-qPCR) of miR-29a, miR- 29b, and miR-29c in the livers of HFD-fed (n = 3) and LFD-fed (n = 3) C57BL/6J female mice are shown. P values were calculated according to the one-tailed unpaired Student t test. a.u., arbitrary unit. *P < 0.05; **P < 0.01; ***P < 0.001.

    Techniques Used: Expressing, Sequencing, Quantitative RT-PCR, One-tailed Test

    Related Articles

    cDNA Library Assay:

    Article Title: RNA-binding protein DAZAP1 promotes gastric cancer metastasis by enhancing NOTCH1 and JAG1 mRNA stability
    Article Snippet: .. DAZAP1-bound RNAs were extracted via TRIzol, and a cDNA library was created via the TruSeq RNA library preparation kit (Illumina, Inc. cat. no. RS-122-2201), the quality and integrity of the processed RNA samples were verified using a Agilent 2100 Bioanalyzer (Agilent Technologies) prior to library construction. .. Sequencing was performed on the Illumina HiSeq X Ten platform by ABLife Inc. with 150 bp paired-end sequencing; the sequencing reaction was conducted using the HiSeq X Ten Reagent kit v2.5 (300 cycles; cat. no. FC-510-1003; Illumina, Inc.).

    RNA Library Preparation:

    Article Title: RNA-binding protein DAZAP1 promotes gastric cancer metastasis by enhancing NOTCH1 and JAG1 mRNA stability
    Article Snippet: .. DAZAP1-bound RNAs were extracted via TRIzol, and a cDNA library was created via the TruSeq RNA library preparation kit (Illumina, Inc. cat. no. RS-122-2201), the quality and integrity of the processed RNA samples were verified using a Agilent 2100 Bioanalyzer (Agilent Technologies) prior to library construction. .. Sequencing was performed on the Illumina HiSeq X Ten platform by ABLife Inc. with 150 bp paired-end sequencing; the sequencing reaction was conducted using the HiSeq X Ten Reagent kit v2.5 (300 cycles; cat. no. FC-510-1003; Illumina, Inc.).

    Generated:

    Article Title: Gestational choline supplementation regulates hippocampal granule neuron development and emotion-like behavior.
    Article Snippet: RNA-Sequence (RNA-Seq) and bioinformatics processing of the data Total RNA was extracted from the hippocampi of F1GCS and F1CON mice with 3 mice per group at each age at key postnatal developmental stages, including P0 (newborn), P10 (peak of synaptogenesis), and P30 (near maturity), using the RNeasy Mini Kit (QIAGEN, Germany) for bulk RNA-seq analysis, enabling the capture of transcriptional dynamics across development. .. The RNA quality was assessed using the Bioanalyser 2100 RNA 6000 Nano Kit (Agilent Technologies, USA). mRNA-Seq libraries were generated from total RNA with polyA+ selection of mRNA using the TruSeq RNA Sample Prep Kit v2 (Illumina, USA), and transcriptomes were sequenced using the HiSeq 2000 Sequencing System (Illumina) in paired-end mode. ..

    Article Title: B-type lamins maintain transcriptional homeostasis by spatially controlling chromatin–speckle proximity
    Article Snippet: .. According to the manufacturer’s instructions, the TruSeq mRNA Library Prep Kit (Illumina) generated the RNA-Seq library. .. To be brief, 100 ng of total cells were extracted, and then reverse transcription was carried out using an oligo-dT primer that had an Illumina-compatible sequence at its 5′ end.

    Article Title: Engineering of plant-derived P450scc for de novo biosynthesis of pregnenolone
    Article Snippet: .. Sequencing libraries were generated using the Illumina TruSeq RNA sample preparation kit (Illumina, USA) following the standard protocol. ..

    Selection:

    Article Title: Gestational choline supplementation regulates hippocampal granule neuron development and emotion-like behavior.
    Article Snippet: RNA-Sequence (RNA-Seq) and bioinformatics processing of the data Total RNA was extracted from the hippocampi of F1GCS and F1CON mice with 3 mice per group at each age at key postnatal developmental stages, including P0 (newborn), P10 (peak of synaptogenesis), and P30 (near maturity), using the RNeasy Mini Kit (QIAGEN, Germany) for bulk RNA-seq analysis, enabling the capture of transcriptional dynamics across development. .. The RNA quality was assessed using the Bioanalyser 2100 RNA 6000 Nano Kit (Agilent Technologies, USA). mRNA-Seq libraries were generated from total RNA with polyA+ selection of mRNA using the TruSeq RNA Sample Prep Kit v2 (Illumina, USA), and transcriptomes were sequenced using the HiSeq 2000 Sequencing System (Illumina) in paired-end mode. ..

    Sample Prep:

    Article Title: Gestational choline supplementation regulates hippocampal granule neuron development and emotion-like behavior.
    Article Snippet: RNA-Sequence (RNA-Seq) and bioinformatics processing of the data Total RNA was extracted from the hippocampi of F1GCS and F1CON mice with 3 mice per group at each age at key postnatal developmental stages, including P0 (newborn), P10 (peak of synaptogenesis), and P30 (near maturity), using the RNeasy Mini Kit (QIAGEN, Germany) for bulk RNA-seq analysis, enabling the capture of transcriptional dynamics across development. .. The RNA quality was assessed using the Bioanalyser 2100 RNA 6000 Nano Kit (Agilent Technologies, USA). mRNA-Seq libraries were generated from total RNA with polyA+ selection of mRNA using the TruSeq RNA Sample Prep Kit v2 (Illumina, USA), and transcriptomes were sequenced using the HiSeq 2000 Sequencing System (Illumina) in paired-end mode. ..

    Article Title: Engineering of plant-derived P450scc for de novo biosynthesis of pregnenolone
    Article Snippet: .. Sequencing libraries were generated using the Illumina TruSeq RNA sample preparation kit (Illumina, USA) following the standard protocol. ..

    Article Title: Physical crowding in 3D niche regulates neural stem cell differentiation via Cx43-mediated gap junction communication
    Article Snippet: RNeasy Mini Kit , Qiagen , Cat# 74104. .. TruSeq RNA sample preparation kit , Illumina , Cat# RS-122. ..

    Article Title: Loss of luminal lineage drives resistance to next-generation ERα antagonists in pretreated ER + HER2 - locally-advanced or metastatic breast cancer.
    Article Snippet: The concentration and the integrity of total RNA samples was determined using a 2200 TapeStation instrument (Agilent Technologies). .. 1ug of total RNA was used as an input material for library preparation using TruSeq RNA Sample Preparation Kit v2 (Illumina). .. Library size was confirmed using the 2200 TapeStation and High Sensitivity D1K screen tape (Agilent Technologies) and their concentration was determined by qPCR based method using Library quantification kit (KAPA).

    Sequencing:

    Article Title: Gestational choline supplementation regulates hippocampal granule neuron development and emotion-like behavior.
    Article Snippet: RNA-Sequence (RNA-Seq) and bioinformatics processing of the data Total RNA was extracted from the hippocampi of F1GCS and F1CON mice with 3 mice per group at each age at key postnatal developmental stages, including P0 (newborn), P10 (peak of synaptogenesis), and P30 (near maturity), using the RNeasy Mini Kit (QIAGEN, Germany) for bulk RNA-seq analysis, enabling the capture of transcriptional dynamics across development. .. The RNA quality was assessed using the Bioanalyser 2100 RNA 6000 Nano Kit (Agilent Technologies, USA). mRNA-Seq libraries were generated from total RNA with polyA+ selection of mRNA using the TruSeq RNA Sample Prep Kit v2 (Illumina, USA), and transcriptomes were sequenced using the HiSeq 2000 Sequencing System (Illumina) in paired-end mode. ..

    Article Title: Engineering of plant-derived P450scc for de novo biosynthesis of pregnenolone
    Article Snippet: .. Sequencing libraries were generated using the Illumina TruSeq RNA sample preparation kit (Illumina, USA) following the standard protocol. ..

    Article Title: Fibrin defines tissue stiffness and biomechanical signaling in regenerating zebrafish hearts as revealed by high-resolution stiffness mapping
    Article Snippet: .. CDNA libraries of 4 samples per timepoint were prepared with Illumina TruSeq Stranded Total RNA Library Prep Kit and run on Illumina platform for sequencing. ..

    RNA Sequencing:

    Article Title: Trans-histone crosstalk establishes distinct H3K79 methylation zones with differential transcriptional functions
    Article Snippet: WT and dot1Δ strains were grown in YPD to an OD 600 of 0.5–0.6, after which half of each culture was shifted to 0.15× YP for 4 h. Total RNA was extracted from 50 ml of cells using the hot phenol method, followed by DNase I treatment (Thermo Scientific Fisher). .. RNA sequencing libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit (Illumina) after rRNA was depleted using the Ribo-Zero yeast kit (Epicenter), as recommended by the manufacturers. .. Paired-end sequencing of the 101-mer read length was carried out on an Illumina NextSeq 500 system.

    RNA sequencing:

    Article Title: B-type lamins maintain transcriptional homeostasis by spatially controlling chromatin–speckle proximity
    Article Snippet: .. According to the manufacturer’s instructions, the TruSeq mRNA Library Prep Kit (Illumina) generated the RNA-Seq library. .. To be brief, 100 ng of total cells were extracted, and then reverse transcription was carried out using an oligo-dT primer that had an Illumina-compatible sequence at its 5′ end.



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    a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) <t>PCR</t> demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , <t>RT-PCR</t> of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.
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    Image Search Results


    a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) PCR demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , RT-PCR of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.

    Journal: Nature microbiology

    Article Title: A metabolic pathway for catabolizing levulinic acid in bacteria

    doi: 10.1038/s41564-017-0028-z

    Figure Lengend Snippet: a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) PCR demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , RT-PCR of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.

    Article Snippet: The Promega GoScript RT PCR kit was used to generate cDNA using 1 μL of a 10 μM gene specific oligo (JMR2 for lvaR and JMR287 for lvaA ) instead of the random oligo mixture.

    Techniques: Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Negative Control, Fluorescence, Clone Assay, Plasmid Preparation