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Nanjing Jiancheng Bioengineering Research Institute Co Ltd giemsa staining kit diff-quick
Effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . (A) <t>Giemsa</t> <t>staining</t> was used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . Black arrows indicate activated macrophages and red arrows indicate multinucleated giant cells. (B) Immunofluorescence staining were used to examine F4/80 and CD163 expression to identify the characteristics of activated macrophages and multinucleated giant cells. White arrows indicate activated macrophages and red arrow indicate multinucleated giant cells. Quantification of (C and D) the percentage of activated macrophages and (E and F) the percentage of multinucleated giant cells in the different groups. Data are presented as the mean ± SD of one independent experiment and the independent experiments were repeated in triplicate. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. ns, not significant (P>0.05); VSCC-SCs, verrucous squamous cell carcinoma-associated stromal cells; SCC-SCs, squamous cell carcinoma-associated stromal cells; HDFs, human dermal fibroblasts.
Giemsa Staining Kit Diff Quick, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/giemsa-diff-quick/giemsa+staining+kit+diff+quick/pmc09097767-51-6-10
Average 90 stars, based on 1 article reviews
giemsa staining kit diff-quick - by Bioz Stars, 2026-10
90/100 stars

Images

1) Product Images from "Crosstalk between cancer and different cancer stroma subtypes promotes the infiltration of tumor-associated macrophages into the tumor microenvironment of oral squamous cell carcinoma"

Article Title: Crosstalk between cancer and different cancer stroma subtypes promotes the infiltration of tumor-associated macrophages into the tumor microenvironment of oral squamous cell carcinoma

Journal: International Journal of Oncology

doi: 10.3892/ijo.2022.5368

Effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . (A) Giemsa staining was used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . Black arrows indicate activated macrophages and red arrows indicate multinucleated giant cells. (B) Immunofluorescence staining were used to examine F4/80 and CD163 expression to identify the characteristics of activated macrophages and multinucleated giant cells. White arrows indicate activated macrophages and red arrow indicate multinucleated giant cells. Quantification of (C and D) the percentage of activated macrophages and (E and F) the percentage of multinucleated giant cells in the different groups. Data are presented as the mean ± SD of one independent experiment and the independent experiments were repeated in triplicate. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. ns, not significant (P>0.05); VSCC-SCs, verrucous squamous cell carcinoma-associated stromal cells; SCC-SCs, squamous cell carcinoma-associated stromal cells; HDFs, human dermal fibroblasts.
Figure Legend Snippet: Effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . (A) Giemsa staining was used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . Black arrows indicate activated macrophages and red arrows indicate multinucleated giant cells. (B) Immunofluorescence staining were used to examine F4/80 and CD163 expression to identify the characteristics of activated macrophages and multinucleated giant cells. White arrows indicate activated macrophages and red arrow indicate multinucleated giant cells. Quantification of (C and D) the percentage of activated macrophages and (E and F) the percentage of multinucleated giant cells in the different groups. Data are presented as the mean ± SD of one independent experiment and the independent experiments were repeated in triplicate. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. ns, not significant (P>0.05); VSCC-SCs, verrucous squamous cell carcinoma-associated stromal cells; SCC-SCs, squamous cell carcinoma-associated stromal cells; HDFs, human dermal fibroblasts.

Techniques Used: Activation Assay, In Vitro, Staining, Immunofluorescence, Expressing

Effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages following crosstalk with HSC-3 cells in vitro . (A) Transwell (migration) and Giemsa staining were used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages. Black arrows indicate spindle-shaped macrophages and red arrows indicate round-shaped macrophages. (B) Transwell (migration) and Giemsa staining were used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages following crosstalk with HSC-3 cells in vitro . Black arrows indicate spindle-shaped macrophages and red arrows indicate round-shaped macrophages. (C and D) Quantification of cell migration number in the different groups. Data are shown as the mean ± SD of one independent experiment and the independent experiments were repeated in triplicate. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05 and **** P<0.0001. ns, not significant (P>0.05); VSCC-SCs, verrucous squamous cell carcinoma-associated stromal cells; SCC-SCs, squamous cell carcinoma-associated stromal cells; HDFs, human dermal fibroblasts.
Figure Legend Snippet: Effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages following crosstalk with HSC-3 cells in vitro . (A) Transwell (migration) and Giemsa staining were used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages. Black arrows indicate spindle-shaped macrophages and red arrows indicate round-shaped macrophages. (B) Transwell (migration) and Giemsa staining were used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages following crosstalk with HSC-3 cells in vitro . Black arrows indicate spindle-shaped macrophages and red arrows indicate round-shaped macrophages. (C and D) Quantification of cell migration number in the different groups. Data are shown as the mean ± SD of one independent experiment and the independent experiments were repeated in triplicate. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05 and **** P<0.0001. ns, not significant (P>0.05); VSCC-SCs, verrucous squamous cell carcinoma-associated stromal cells; SCC-SCs, squamous cell carcinoma-associated stromal cells; HDFs, human dermal fibroblasts.

Techniques Used: Migration, In Vitro, Staining

Related Articles

Incubation:

Article Title: Crosstalk between cancer and different cancer stroma subtypes promotes the infiltration of tumor‑associated macrophages into the tumor microenvironment of oral squamous cell carcinoma.
Article Snippet: The RAW264.7 cells were seeded into the upper chamber of 8‐μm Transwell filters without Matrigel in 24‐well plates (Corning, Falcon cell culture inserts; BD Biosciences) in α‐MEM without FBS at a density of 2x104 cells/500 μl. .. Following incubation for 1 day at 37 ̊C in a humidified atmo‐ sphere of 5% CO2 and 95% air, the upper chambers were stained using the Giemsa staining kit (Diff‐Quick, Nanjing Jiancheng Bioengineering Institute). ..

Article Title: The Origin of Stroma Influences the Biological Characteristics of Oral Squamous Cell Carcinoma
Article Snippet: .. After incubation for 1 d, the slides, with the cells attached, were stained with the Giemsa Staining Kit (Diff-Quick, Nanjing Jiancheng Bioengineering Institute, Nanjing, China). .. The stained cells were photographed using a bright field microscope (BX51, Olympus, Tokyo, Japan), and the experiment was repeated three times, independently.

Staining:

Article Title: Crosstalk between cancer and different cancer stroma subtypes promotes the infiltration of tumor‑associated macrophages into the tumor microenvironment of oral squamous cell carcinoma.
Article Snippet: The RAW264.7 cells were seeded into the upper chamber of 8‐μm Transwell filters without Matrigel in 24‐well plates (Corning, Falcon cell culture inserts; BD Biosciences) in α‐MEM without FBS at a density of 2x104 cells/500 μl. .. Following incubation for 1 day at 37 ̊C in a humidified atmo‐ sphere of 5% CO2 and 95% air, the upper chambers were stained using the Giemsa staining kit (Diff‐Quick, Nanjing Jiancheng Bioengineering Institute). ..

Article Title: The Origin of Stroma Influences the Biological Characteristics of Oral Squamous Cell Carcinoma
Article Snippet: .. After incubation for 1 d, the slides, with the cells attached, were stained with the Giemsa Staining Kit (Diff-Quick, Nanjing Jiancheng Bioengineering Institute, Nanjing, China). .. The stained cells were photographed using a bright field microscope (BX51, Olympus, Tokyo, Japan), and the experiment was repeated three times, independently.

Article Title: Crosstalk between cancer and different cancer stroma subtypes promotes the infiltration of tumor-associated macrophages into the tumor microenvironment of oral squamous cell carcinoma
Article Snippet: .. Giemsa staining was conducted using a Giemsa staining kit (Diff-Quick, Nanjing Jiancheng Bioengineering Institute). ..

Article Title: Crosstalk between cancer and different cancer stroma subtypes promotes the infiltration of tumor‑associated macrophages into the tumor microenvironment of oral squamous cell carcinoma.
Article Snippet: .. Giemsa staining was conducted using a Giemsa staining kit (Diff‐Quick, Nanjing Jiancheng Bioengineering Institute). ..

Diff-Quik:

Article Title: Crosstalk between cancer and different cancer stroma subtypes promotes the infiltration of tumor‑associated macrophages into the tumor microenvironment of oral squamous cell carcinoma.
Article Snippet: The RAW264.7 cells were seeded into the upper chamber of 8‐μm Transwell filters without Matrigel in 24‐well plates (Corning, Falcon cell culture inserts; BD Biosciences) in α‐MEM without FBS at a density of 2x104 cells/500 μl. .. Following incubation for 1 day at 37 ̊C in a humidified atmo‐ sphere of 5% CO2 and 95% air, the upper chambers were stained using the Giemsa staining kit (Diff‐Quick, Nanjing Jiancheng Bioengineering Institute). ..

Article Title: The Origin of Stroma Influences the Biological Characteristics of Oral Squamous Cell Carcinoma
Article Snippet: .. After incubation for 1 d, the slides, with the cells attached, were stained with the Giemsa Staining Kit (Diff-Quick, Nanjing Jiancheng Bioengineering Institute, Nanjing, China). .. The stained cells were photographed using a bright field microscope (BX51, Olympus, Tokyo, Japan), and the experiment was repeated three times, independently.

Article Title: Crosstalk between cancer and different cancer stroma subtypes promotes the infiltration of tumor-associated macrophages into the tumor microenvironment of oral squamous cell carcinoma
Article Snippet: .. Giemsa staining was conducted using a Giemsa staining kit (Diff-Quick, Nanjing Jiancheng Bioengineering Institute). ..

Article Title: Crosstalk between cancer and different cancer stroma subtypes promotes the infiltration of tumor‑associated macrophages into the tumor microenvironment of oral squamous cell carcinoma.
Article Snippet: .. Giemsa staining was conducted using a Giemsa staining kit (Diff‐Quick, Nanjing Jiancheng Bioengineering Institute). ..



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Effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . (A) <t>Giemsa</t> <t>staining</t> was used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . Black arrows indicate activated macrophages and red arrows indicate multinucleated giant cells. (B) Immunofluorescence staining were used to examine F4/80 and CD163 expression to identify the characteristics of activated macrophages and multinucleated giant cells. White arrows indicate activated macrophages and red arrow indicate multinucleated giant cells. Quantification of (C and D) the percentage of activated macrophages and (E and F) the percentage of multinucleated giant cells in the different groups. Data are presented as the mean ± SD of one independent experiment and the independent experiments were repeated in triplicate. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. ns, not significant (P>0.05); VSCC-SCs, verrucous squamous cell carcinoma-associated stromal cells; SCC-SCs, squamous cell carcinoma-associated stromal cells; HDFs, human dermal fibroblasts.
Giemsa Staining Kit Diff Quick, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/giemsa-diff-quick/giemsa+staining+kit+diff+quick/pmc09097767-51-6-10
Average 90 stars, based on 1 article reviews
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Image Search Results


Effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . (A) Giemsa staining was used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . Black arrows indicate activated macrophages and red arrows indicate multinucleated giant cells. (B) Immunofluorescence staining were used to examine F4/80 and CD163 expression to identify the characteristics of activated macrophages and multinucleated giant cells. White arrows indicate activated macrophages and red arrow indicate multinucleated giant cells. Quantification of (C and D) the percentage of activated macrophages and (E and F) the percentage of multinucleated giant cells in the different groups. Data are presented as the mean ± SD of one independent experiment and the independent experiments were repeated in triplicate. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. ns, not significant (P>0.05); VSCC-SCs, verrucous squamous cell carcinoma-associated stromal cells; SCC-SCs, squamous cell carcinoma-associated stromal cells; HDFs, human dermal fibroblasts.

Journal: International Journal of Oncology

Article Title: Crosstalk between cancer and different cancer stroma subtypes promotes the infiltration of tumor-associated macrophages into the tumor microenvironment of oral squamous cell carcinoma

doi: 10.3892/ijo.2022.5368

Figure Lengend Snippet: Effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . (A) Giemsa staining was used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . Black arrows indicate activated macrophages and red arrows indicate multinucleated giant cells. (B) Immunofluorescence staining were used to examine F4/80 and CD163 expression to identify the characteristics of activated macrophages and multinucleated giant cells. White arrows indicate activated macrophages and red arrow indicate multinucleated giant cells. Quantification of (C and D) the percentage of activated macrophages and (E and F) the percentage of multinucleated giant cells in the different groups. Data are presented as the mean ± SD of one independent experiment and the independent experiments were repeated in triplicate. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. ns, not significant (P>0.05); VSCC-SCs, verrucous squamous cell carcinoma-associated stromal cells; SCC-SCs, squamous cell carcinoma-associated stromal cells; HDFs, human dermal fibroblasts.

Article Snippet: Giemsa staining was conducted using a Giemsa staining kit (Diff-Quick, Nanjing Jiancheng Bioengineering Institute).

Techniques: Activation Assay, In Vitro, Staining, Immunofluorescence, Expressing

Effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages following crosstalk with HSC-3 cells in vitro . (A) Transwell (migration) and Giemsa staining were used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages. Black arrows indicate spindle-shaped macrophages and red arrows indicate round-shaped macrophages. (B) Transwell (migration) and Giemsa staining were used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages following crosstalk with HSC-3 cells in vitro . Black arrows indicate spindle-shaped macrophages and red arrows indicate round-shaped macrophages. (C and D) Quantification of cell migration number in the different groups. Data are shown as the mean ± SD of one independent experiment and the independent experiments were repeated in triplicate. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05 and **** P<0.0001. ns, not significant (P>0.05); VSCC-SCs, verrucous squamous cell carcinoma-associated stromal cells; SCC-SCs, squamous cell carcinoma-associated stromal cells; HDFs, human dermal fibroblasts.

Journal: International Journal of Oncology

Article Title: Crosstalk between cancer and different cancer stroma subtypes promotes the infiltration of tumor-associated macrophages into the tumor microenvironment of oral squamous cell carcinoma

doi: 10.3892/ijo.2022.5368

Figure Lengend Snippet: Effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages following crosstalk with HSC-3 cells in vitro . (A) Transwell (migration) and Giemsa staining were used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages. Black arrows indicate spindle-shaped macrophages and red arrows indicate round-shaped macrophages. (B) Transwell (migration) and Giemsa staining were used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages following crosstalk with HSC-3 cells in vitro . Black arrows indicate spindle-shaped macrophages and red arrows indicate round-shaped macrophages. (C and D) Quantification of cell migration number in the different groups. Data are shown as the mean ± SD of one independent experiment and the independent experiments were repeated in triplicate. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05 and **** P<0.0001. ns, not significant (P>0.05); VSCC-SCs, verrucous squamous cell carcinoma-associated stromal cells; SCC-SCs, squamous cell carcinoma-associated stromal cells; HDFs, human dermal fibroblasts.

Article Snippet: Giemsa staining was conducted using a Giemsa staining kit (Diff-Quick, Nanjing Jiancheng Bioengineering Institute).

Techniques: Migration, In Vitro, Staining