microgrid ii microarray spotter (Zinsser Analytic)

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Zinsser Analytic microgrid ii microarray spotter
Biotype and genome location of ncRNAs from the neuro-ncRNA <t>microarray.</t> ncRNAs are sorted by ncRNA biotype and genomic locations. Frequencies are illustrated in log 2 scale. Frequency of zero is indicated in gray. Strandness of ncRNAs is included in the biotype names (sense: “_SE,” antisense: “_AS”). Biotypes follow GENCODE/ENSEMBL annotation. Missing annotations are designated as “unknown.”

Biotype and genome location of ncRNAs from the neuro-ncRNA <t>microarray.</t> ncRNAs are sorted by ncRNA biotype and genomic locations. Frequencies are illustrated in log 2 scale. Frequency of zero is indicated in gray. Strandness of ncRNAs is included in the biotype names (sense: “_SE,” antisense: “_AS”). Biotypes follow GENCODE/ENSEMBL annotation. Missing annotations are designated as “unknown.”

Microgrid Ii Microarray Spotter, supplied by Zinsser Analytic, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/generation+iii+microarray+spotter+device/microgrid+ii+microarrayer/pmc04238357-188-7-11
Average 90 stars, based on 1 article reviews

microgrid ii microarray spotter - by Bioz Stars, 2026-10

90/100 stars

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1) Product Images from "Generation of a neuro-specific microarray reveals novel differentially expressed noncoding RNAs in mouse models for neurodegenerative diseases"

Article Title: Generation of a neuro-specific microarray reveals novel differentially expressed noncoding RNAs in mouse models for neurodegenerative diseases

Journal: RNA

doi: 10.1261/rna.047225.114

Biotype and genome location of ncRNAs from the neuro-ncRNA microarray. ncRNAs are sorted by ncRNA biotype and genomic locations. Frequencies are illustrated in log 2 scale. Frequency of zero is indicated in gray. Strandness of ncRNAs is included in the biotype names (sense: “_SE,” antisense: “_AS”). Biotypes follow GENCODE/ENSEMBL annotation. Missing annotations are designated as “unknown.”

Figure Legend Snippet: Biotype and genome location of ncRNAs from the neuro-ncRNA microarray. ncRNAs are sorted by ncRNA biotype and genomic locations. Frequencies are illustrated in log 2 scale. Frequency of zero is indicated in gray. Strandness of ncRNAs is included in the biotype names (sense: “_SE,” antisense: “_AS”). Biotypes follow GENCODE/ENSEMBL annotation. Missing annotations are designated as “unknown.”

Techniques Used: Microarray

Comparison of expression analysis between Northern blot (black bars) and microarray analysis (gray bars) of 14 randomly chosen ncRNAs. ( A ) Expression changes of ncRNAs between brain and muscle tissues. ( B ) Expression changes of ncRNAs between brain and liver tissues. Candidate IDs are indicated on top . Details are listed in Supplemental Table 2. Fold changes from Northern blot analysis were quantified by using ImageQuant 8.1 (GE Healthcare).

Figure Legend Snippet: Comparison of expression analysis between Northern blot (black bars) and microarray analysis (gray bars) of 14 randomly chosen ncRNAs. ( A ) Expression changes of ncRNAs between brain and muscle tissues. ( B ) Expression changes of ncRNAs between brain and liver tissues. Candidate IDs are indicated on top . Details are listed in Supplemental Table 2. Fold changes from Northern blot analysis were quantified by using ImageQuant 8.1 (GE Healthcare).

Techniques Used: Comparison, Expressing, Northern Blot, Microarray

Differential expression analysis of ncRNAs from selected brain regions of mouse models for voltage-gated calcium channel activity compared with wild type. ( A ) Cacnb4 mutant mouse: cerebellum. ( B ) Ca V 1.3 −/− : hippocampus. ( C ) Ca V 1.3 −/− : striatum. Box plots represent fold changes of ncRNAs (colored data points), which showed significant changes in expression relative to wild-type controls in microarray experiments. Fold changes are illustrated in log 2 scale. Whiskers extend to ±1.5-fold of the interquartile range (IQR). Biotypes are designated by color code: miRNAs (red), snoRNA (orange), tRF (blue), other biotypes (green), and unknown biotype (gray). ( D ) Venn diagram illustrating the overlap of differentially expressed candidates between the models analyzed. ( E ) Fold change and annotation of ncRNA candidates highlighted in D .

Figure Legend Snippet: Differential expression analysis of ncRNAs from selected brain regions of mouse models for voltage-gated calcium channel activity compared with wild type. ( A ) Cacnb4 mutant mouse: cerebellum. ( B ) Ca V 1.3 −/− : hippocampus. ( C ) Ca V 1.3 −/− : striatum. Box plots represent fold changes of ncRNAs (colored data points), which showed significant changes in expression relative to wild-type controls in microarray experiments. Fold changes are illustrated in log 2 scale. Whiskers extend to ±1.5-fold of the interquartile range (IQR). Biotypes are designated by color code: miRNAs (red), snoRNA (orange), tRF (blue), other biotypes (green), and unknown biotype (gray). ( D ) Venn diagram illustrating the overlap of differentially expressed candidates between the models analyzed. ( E ) Fold change and annotation of ncRNA candidates highlighted in D .

Techniques Used: Quantitative Proteomics, Activity Assay, Mutagenesis, Expressing, Microarray

Differential expression of ncRNAs from cortices of a triple-transgenic (3xTG) mouse model for Alzheimer's disease compared with wild-type controls at the age of 3, 10, and 20 mo, respectively. ( A ) Box plots represent fold changes of ncRNAs (colored data points), which showed significant changes in expression relative to their wild-type controls in microarray experiments. The experiments are illustrated from left to right : 3-, 10-, and 20-mo-old mice. Fold changes are illustrated in log 2 scale. Whiskers extend to ±1.5-fold of the interquartile range (IQR). Biotypes are designated by color code: miRNAs (red), snoRNA (orange), tRF (blue), other biotypes (green), and unknown biotype (gray). ( B ) Venn diagram illustrating the overlap of differentially expressed candidates between the different age groups. ( C ) Fold change and annotation of ncRNA candidates highlighted in B .

Figure Legend Snippet: Differential expression of ncRNAs from cortices of a triple-transgenic (3xTG) mouse model for Alzheimer's disease compared with wild-type controls at the age of 3, 10, and 20 mo, respectively. ( A ) Box plots represent fold changes of ncRNAs (colored data points), which showed significant changes in expression relative to their wild-type controls in microarray experiments. The experiments are illustrated from left to right : 3-, 10-, and 20-mo-old mice. Fold changes are illustrated in log 2 scale. Whiskers extend to ±1.5-fold of the interquartile range (IQR). Biotypes are designated by color code: miRNAs (red), snoRNA (orange), tRF (blue), other biotypes (green), and unknown biotype (gray). ( B ) Venn diagram illustrating the overlap of differentially expressed candidates between the different age groups. ( C ) Fold change and annotation of ncRNA candidates highlighted in B .

Techniques Used: Quantitative Proteomics, Transgenic Assay, Expressing, Microarray

Differentially expressed snoRNAs in young 3xTG AD mice. ( A ) Candidate e307: sequence similarity of mouse (mm9 assembly) to the human (hg19 assembly) snoRNA homologs (located on chromosome 5), and ( left ) predicted secondary structure illustrated by VARNA ( http://varna.lri.fr/ ). ( Right ) Northern blot analysis of e307 from total RNA of cortices of 10-mo-old 3xTG mice compared with wild-type controls. ( B ) Candidate e470: genome positions of the two (red and green bars) potential loci of transcription (on chromosome 5), which differ by a single SNP (indicated in bold). Sequence similarity of HBII-336 and MBII-336; ( left ) predicted secondary structure illustrated by VARNA. ( Right ) Northern blot analysis from total RNA of cortices of 10-mo-old 3xTG mice compared with wild-type controls. C/D boxes are illustrated in red and sequences targeted by the oligonucleotide from the microarray e307 and e470, respectively, are highlighted in blue.

Figure Legend Snippet: Differentially expressed snoRNAs in young 3xTG AD mice. ( A ) Candidate e307: sequence similarity of mouse (mm9 assembly) to the human (hg19 assembly) snoRNA homologs (located on chromosome 5), and ( left ) predicted secondary structure illustrated by VARNA ( http://varna.lri.fr/ ). ( Right ) Northern blot analysis of e307 from total RNA of cortices of 10-mo-old 3xTG mice compared with wild-type controls. ( B ) Candidate e470: genome positions of the two (red and green bars) potential loci of transcription (on chromosome 5), which differ by a single SNP (indicated in bold). Sequence similarity of HBII-336 and MBII-336; ( left ) predicted secondary structure illustrated by VARNA. ( Right ) Northern blot analysis from total RNA of cortices of 10-mo-old 3xTG mice compared with wild-type controls. C/D boxes are illustrated in red and sequences targeted by the oligonucleotide from the microarray e307 and e470, respectively, are highlighted in blue.

Techniques Used: Sequencing, Northern Blot, Microarray

Microarray:

Article Title: Generation of a neuro-specific microarray reveals novel differentially expressed noncoding RNAs in mouse models for neurodegenerative diseases
Article Snippet: .. The neuro-ncRNA microarray was generated using the MicroGrid II Microarray Spotter (Zinsser Analytic). .. Thereby, 3977 oligonucleotides were dissolved in spotting buffer consisting of 3× SSC, 1.5 M betaine, yielding a concentration of 25 μm, and spotted on HiSens epoxy-coated glass slides (SCHOTT).

Article Title: Genome-wide gene expression analysis of the switch between acidogenesis and solventogenesis in continuous cultures of Clostridium acetobutylicum.
Article Snippet: The integrity of the RNA was checked by RTPCR of ATPase genes after ensuring absence of contaminating DNA by PCR and additionally controlled in a BioAnalyzer (Agilent, Böblingen, Germany) run. .. DNA Microarray The C. acetobutylicum array was constructed by spotting 5 amino-C6-modified oligonucleotides with a length of 60–70 bases on CodeLink microarray slides (SurModics, Eden Prairie, Minn., USA) using a MicroGrid II microarray spotter (Zinsser Analytic, Frankfurt, Germany). ..

Article Title: Expression Profiling of a Heterogeneous Population of ncRNAs Employing a Mixed DNA/LNA Microarray
Article Snippet: .. The LNA-based capture probe set for short ncRNAs as well as the self-designed DNA-based capture probe set for long ncRNAs was spotted on HiSens epoxy-coated glass slides (Nexterion) using the MicroGrid II Microarray Spotter (Zinsser Analytic). ..

Article Title: RACK1/Asc1p, a Ribosomal Node in Cellular Signaling
Article Snippet: Microarray Analysis The array was constructed by spotting a 15 μ m solution of 5′ amino-C6-modified oligonucleotides in 50 m m sodium phosphate pH 8.5 with a length of 70 bases on CodeLink microarray slides (SurModics, Eden Prairie, MN) using a MicroGrid II microarray spotter (Zinsser Analytic, Frankfurt, Germany). .. Microarray Analysis The array was constructed by spotting a 15 μ m solution of 5′ amino-C6-modified oligonucleotides in 50 m m sodium phosphate pH 8.5 with a length of 70 bases on CodeLink microarray slides (SurModics, Eden Prairie, MN) using a MicroGrid II microarray spotter (Zinsser Analytic, Frankfurt, Germany). ..

Generated:

Produced:

Article Title: Identification of differential and functionally active miRNAs in both anaplastic lymphoma kinase (ALK) + and ALK − anaplastic large-cell lymphoma
Article Snippet: .. miRNA chips were produced by spotting the LNA-modified miRCURY LNA miRNA Array ready-to-spot probe set no. 208010-A (Exiqon), which consists of 2,056 capture probes designed to have a uniform temperature of 72 °C and covers all miRNAs of miRBase version 9.2 ( http://www.mirbase.org/ ) in eight replicates on Hisens epoxy-coated glass slides (Nexterion) using the MicroGrid II Microarrayer (Zinsser Analytic). .. Microarrays with immobilized LNA-modified capture probes were hybridized for 16 h at 65 °C on a Slide Booster (Advalytix) in hybridization buffer, followed by three washes in buffers of increasing stringency according to the manufacturer's instructions (Exiqon).

Construct:

other:

Article Title: UCP1 (thermogenin)—inducing agents for use in the treatment of a disorder of the energy homeostasis
Article Snippet: Two cell populations, termed hMADS-2 and hMADS-3 cells respectively, were used for cell culture experiments which were proliferated in 100 mm cell culture dishes (Greiner Bio-One, Cat. no. 664160).

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