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Gene expression levels determined by the cDNA <t>microarray</t> and real time PCR methods for RHOA , LIMK and TIMP2. The same RNA samples were used in both methods. The ΔΔct-values represent the log ratio (base 2).
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generation iii microarrays spotter device  (Amersham Pharmacia Biotech Ltd)
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Gene expression levels determined by the cDNA <t>microarray</t> and real time PCR methods for RHOA , LIMK and TIMP2. The same RNA samples were used in both methods. The ΔΔct-values represent the log ratio (base 2).
Generation Iii Microarrays Spotter Device, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Gene expression levels determined by the cDNA microarray and real time PCR methods for RHOA , LIMK and TIMP2. The same RNA samples were used in both methods. The ΔΔct-values represent the log ratio (base 2).

Journal: Genetics and Molecular Biology

Article Title: Alterations in gene expression profiles correlated with cisplatin cytotoxicity in the glioma U343 cell line

doi: 10.1590/S1415-47572010005000013

Figure Lengend Snippet: Gene expression levels determined by the cDNA microarray and real time PCR methods for RHOA , LIMK and TIMP2. The same RNA samples were used in both methods. The ΔΔct-values represent the log ratio (base 2).

Article Snippet: The PCR products were purified and spotted in duplicate onto Hybond N+ membranes (Amersham Pharmacia Biotech, Buckinghamshire, UK), using the Generation III Microarray Spotter device (Amersham Pharmacia Biotech, Buckinghamshire, UK).

Techniques: Expressing, Microarray, Real-time Polymerase Chain Reaction