gene expression microarray experiments (Welgene Biotech)

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Welgene Biotech gene expression microarray experiments

ABCG2 upregulation is associated with SIK3 attenuation. (A) List of ABC family proteins from <t>microarray</t> data for which the expression changed more than 1.5-fold compared with control cells. (B) The mRNA expression levels of ABC proteins were verified by real-time PCR in OVCAR4 and SKOV3 cells. The white bar (shLuc) represents control cells, and the gray bar (shSIK3#01) and black bar (shSIK3#61) represent indicated cells with SIK3 knockdown. Data are represented as the mean ± SEM from three independent experiments and analyzed by one-way ANOVA. *: p <0.05 and **: p <0.01 (compared to the shLuc control). Note that ABCG1 and ABCG2 were both significantly upregulated in cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (C) The protein expression levels of ABCG1 and ABCG2 were further verified by Western blotting. Note that only ABCG2 was upregulated in OVCAR4 and SKOV3 cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (D) The functional MDR activity of the ABCG2 protein was determined using an MDR assay kit (Abcam). A total of 2 x 10 5 suspended cells were pretreated with the inhibitor novobiocin (50 nM) or DMSO. Then, diluted Efflux Gold Detection Reagent was added at 37°C for 30 minutes. The cellular orange fluorescence signal of the Efflux Gold Detection Reagent was measured immediately by flow cytometry in the living (PI-negative) cell population. The numbers in the upper left corners are MAF values.

Gene Expression Microarray Experiments, supplied by Welgene Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+expression+microarray+experiments/microarray+analysis/pmc06856590-85-14-9
Average 90 stars, based on 1 article reviews

gene expression microarray experiments - by Bioz Stars, 2026-09

90/100 stars

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1) Product Images from "Downregulated Salt-inducible Kinase 3 Expression Promotes Chemoresistance in Serous Ovarian Cancer via the ATP‐binding Cassette Protein ABCG2"

Article Title: Downregulated Salt-inducible Kinase 3 Expression Promotes Chemoresistance in Serous Ovarian Cancer via the ATP‐binding Cassette Protein ABCG2

Journal: Journal of Cancer

doi: 10.7150/jca.34886

ABCG2 upregulation is associated with SIK3 attenuation. (A) List of ABC family proteins from microarray data for which the expression changed more than 1.5-fold compared with control cells. (B) The mRNA expression levels of ABC proteins were verified by real-time PCR in OVCAR4 and SKOV3 cells. The white bar (shLuc) represents control cells, and the gray bar (shSIK3#01) and black bar (shSIK3#61) represent indicated cells with SIK3 knockdown. Data are represented as the mean ± SEM from three independent experiments and analyzed by one-way ANOVA. *: p <0.05 and **: p <0.01 (compared to the shLuc control). Note that ABCG1 and ABCG2 were both significantly upregulated in cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (C) The protein expression levels of ABCG1 and ABCG2 were further verified by Western blotting. Note that only ABCG2 was upregulated in OVCAR4 and SKOV3 cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (D) The functional MDR activity of the ABCG2 protein was determined using an MDR assay kit (Abcam). A total of 2 x 10 5 suspended cells were pretreated with the inhibitor novobiocin (50 nM) or DMSO. Then, diluted Efflux Gold Detection Reagent was added at 37°C for 30 minutes. The cellular orange fluorescence signal of the Efflux Gold Detection Reagent was measured immediately by flow cytometry in the living (PI-negative) cell population. The numbers in the upper left corners are MAF values.

Figure Legend Snippet: ABCG2 upregulation is associated with SIK3 attenuation. (A) List of ABC family proteins from microarray data for which the expression changed more than 1.5-fold compared with control cells. (B) The mRNA expression levels of ABC proteins were verified by real-time PCR in OVCAR4 and SKOV3 cells. The white bar (shLuc) represents control cells, and the gray bar (shSIK3#01) and black bar (shSIK3#61) represent indicated cells with SIK3 knockdown. Data are represented as the mean ± SEM from three independent experiments and analyzed by one-way ANOVA. *: p <0.05 and **: p <0.01 (compared to the shLuc control). Note that ABCG1 and ABCG2 were both significantly upregulated in cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (C) The protein expression levels of ABCG1 and ABCG2 were further verified by Western blotting. Note that only ABCG2 was upregulated in OVCAR4 and SKOV3 cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (D) The functional MDR activity of the ABCG2 protein was determined using an MDR assay kit (Abcam). A total of 2 x 10 5 suspended cells were pretreated with the inhibitor novobiocin (50 nM) or DMSO. Then, diluted Efflux Gold Detection Reagent was added at 37°C for 30 minutes. The cellular orange fluorescence signal of the Efflux Gold Detection Reagent was measured immediately by flow cytometry in the living (PI-negative) cell population. The numbers in the upper left corners are MAF values.

Techniques Used: Microarray, Expressing, Control, Real-time Polymerase Chain Reaction, Knockdown, Western Blot, Functional Assay, Activity Assay, Fluorescence, Flow Cytometry

Microarray:

Article Title: Increased Serum Levels of Brain-Derived Neurotrophic Factor Contribute to Inflammatory Responses in Patients with Rheumatoid Arthritis
Article Snippet: .. The microarray analysis was performed by Welgene Biotech (Taipei, Taiwan) as in our previous study [ ]. .. In brief, total RNA was extracted by TRIzol reagent (Invitrogen, Carlsbad, CA, USA), then quantified at OD 260nm by an ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and quantified using a Bioanalyzer 2100 (Agilent Technology, USA) with an RNA 6000 lab chip kit (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Second harmonic generation microscopy reveals the spatial orientation of glutamine-potentiated liver regeneration after hepatectomy
Article Snippet: .. Liver samples were prepared for mRNA expression microarray from day 1 to day 6 after PHx by Welgene Biotech Co. Ltd. .. Briefly, microarray analyses were performed using the SurePrint G3 GeneChip Mouse Gene Express V2 Array Kit (Agilent) according to the manufacturer’s protocols.

Article Title: Switched phenotypes of macrophages during the different stages of Schistosoma japonicum infection influenced the subsequent trends of immune responses.
Article Snippet: .. The microarray experiment was also commissioned to Welgene Biotech, Co., Ltd. to make the experimental analysis of gene arrays. .. This experiment adopted Agilent Technologies, Inc. (CA) and Agilent custom mouse immune arrays.

Article Title: Second harmonic generation microscopy reveals the spatial orientation of glutamine-potentiated liver regeneration after hepatectomy
Article Snippet: .. Gene expression microarray and gene set enrichment analysis Liver samples were prepared for mRNA expression microarray from day 1 to day 6 after PHx by Welgene Biotech Co. Ltd. .. Briefly, microarray analyses were performed using the SurePrint G3 GeneChip Mouse Gene Express V2 Array Kit (Agilent) according to the manufacturer’s protocols.

Article Title: Ultrarapid Inflammation of the Olfactory Bulb After Spinal Cord Injury: Protective Effects of the Granulocyte Colony-Stimulating Factor on Early Neurodegeneration in the Brain
Article Snippet: .. Microarray analysis was performed using the Agilent mouse gene expression microarray kit (Welgene Biotech, Taipei, Taiwan), following the manufacturer’s instructions. .. Briefly, 0.2 μg of total RNA extracted from the whole-brain lysates was labeled with Cy3 (CyDye, Agilent Technologies, Santa Clara, CA, United States) using the Low Input Quick-Amp labeling kit (Agilent Technologies, Santa Clara, CA, United States) during the in vitro transcription process.

Article Title: Increased Expression of Long Noncoding RNA LOC100506314 in T cells from Patients with Nonsegmental Vitiligo and Its Contribution to Vitiligo Pathogenesis
Article Snippet: Then anti-human CD4-coated magnetic beads and anti-human CD8-coated magnetic beads were used to separate the T cells, CD4+ T cells, or CD8+ T cells by IMag cell separation system (BD Bioscience, Franklin Lakes, NJ, USA). .. The gene expression profiles of T cells obtained from three female vitiligo patients aged 44, 46, and 48 years and three female controls aged 45, 47, and 48 years were investigated using the microarray analysis (Welgene Biotech, Taipei, Taiwan) as previously described [ ]. .. We extracted total RNA by the TRIzol reagent (Invitrogen, Carlsbad, CA, USA).

Article Title: Downregulated Salt-inducible Kinase 3 Expression Promotes Chemoresistance in Serous Ovarian Cancer via the ATP‐binding Cassette Protein ABCG2
Article Snippet: .. TRIzol-isolated RNA samples were shipped on dry ice to Welgene Biotech (Taiwan), where the gene expression microarray experiments were performed as a contract service. .. The labeling of 100 ng of total RNA was performed using the Low Input Quick Amp Labeling Kit (Agilent Technologies).

Expressing:

Article Title: Second harmonic generation microscopy reveals the spatial orientation of glutamine-potentiated liver regeneration after hepatectomy
Article Snippet: .. Gene expression microarray and gene set enrichment analysis Liver samples were prepared for mRNA expression microarray from day 1 to day 6 after PHx by Welgene Biotech Co. Ltd. .. Briefly, microarray analyses were performed using the SurePrint G3 GeneChip Mouse Gene Express V2 Array Kit (Agilent) according to the manufacturer’s protocols.

Gene Expression:

Article Title: Second harmonic generation microscopy reveals the spatial orientation of glutamine-potentiated liver regeneration after hepatectomy
Article Snippet: .. Gene expression microarray and gene set enrichment analysis Liver samples were prepared for mRNA expression microarray from day 1 to day 6 after PHx by Welgene Biotech Co. Ltd. .. Briefly, microarray analyses were performed using the SurePrint G3 GeneChip Mouse Gene Express V2 Array Kit (Agilent) according to the manufacturer’s protocols.

other:

Article Title: The AtERF19 gene regulates meristem activity and flower organ size in plants.
Article Snippet: Ethylene-responsive factors (ERFs) have diverse functions in the regulation of various plant developmental processes.. Here, we demonstrate the dual role of an Arabidopsis ERF gene, AtERF19, in regulating reproductive meristem activity and flower organ size through the regulation of genes involved in CLAVATA– WUSCHEL (CLV–WUS) and auxin signaling, respectively.. We found that AtERF19 stimulated the formation of flower primordia and controlled the number of flowers produced by activating WUS and was negatively regulated by CLV3.

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