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Arraystar inc human gene expression microarray
Human Gene Expression Microarray, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+expression+microarray+experiments/human+gene+expression+microarray/pm39881131-132-35-45
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human gene expression microarray - by Bioz Stars, 2026-09
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Labeling:

Article Title: KIF26B, a novel oncogene, promotes proliferation and metastasis by activating the VEGF pathway in gastric cancer.
Article Snippet: Tumor metastasis is the main reason of cancer-related death for gastric cancer (GC) patients and gene expression microarray data indicate that kinesin family member 26B (KIF26B) is one of the most upregulated genes in metastatic GC samples.. Specifically, KIF26B expression was upregulated in a stepwise manner from non-tumorous gastric mucosa, primary GC tissues without metastasis, via primary GC tissues with metastasis, to secondary lymph node metastatic (LNM) foci.. Increased expression of KIF26B was correlated with tumor size, positive LNM or distant metastases and poor prognosis.

Gene Expression:

Article Title: KIF26B, a novel oncogene, promotes proliferation and metastasis by activating the VEGF pathway in gastric cancer.
Article Snippet: Tumor metastasis is the main reason of cancer-related death for gastric cancer (GC) patients and gene expression microarray data indicate that kinesin family member 26B (KIF26B) is one of the most upregulated genes in metastatic GC samples.. Specifically, KIF26B expression was upregulated in a stepwise manner from non-tumorous gastric mucosa, primary GC tissues without metastasis, via primary GC tissues with metastasis, to secondary lymph node metastatic (LNM) foci.. Increased expression of KIF26B was correlated with tumor size, positive LNM or distant metastases and poor prognosis.

Article Title: LncRNA PCAT1 and its genetic variant rs1902432 are associated with prostate cancer risk
Article Snippet: Total RNA was extracted from DU145 PCa cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. .. The mRNA of three pairs of DU145 cells transfected with PCAT1 overexpression vector or corresponding NC vector was profiled with Arraystar Human Gene Expression Array. ..

Article Title: SP-1-activated LINC01016 overexpression promotes gastric cancer invasion and metastasis through inhibiting EIF4A3-mediated MMP9 mRNA decay
Article Snippet: .. First, differential gene expression analysis of lncRNAs was performed between five cases of gastric cancers with LNM and another five cases without LNM using the Human Gene Expression Microarray (Human 8×60 K LncRNA Microarray v2.0; Arraystar, Rockville, USA). ..

Article Title: SP-1-activated LINC01016 overexpression promotes gastric cancer invasion and metastasis through inhibiting EIF4A3-mediated MMP9 mRNA decay.
Article Snippet: .. RESULTS LINC01016 is upregulated in GC with lymph node metastasis First, differential gene expression analysis of lncRNAs was performed between five cases of gastric cancers with LNM and another five cases without LNM using the Human Gene Expression Microarray (Human 8×60 K LncRNA Microarray v2.0; Arraystar, Rockville, USA). ..

Article Title: LncRNA PCAT1 and its genetic variant rs1902432 are associated with prostate cancer risk
Article Snippet: .. Arraystar Human Gene Expression Array was conducted to assess the genome-wide profiling of gene expression from PCa cell DU145 transfected with PCAT1 overexpression vector or NC vector. ..

Article Title: Long noncoding RNA lnc-LEMGC combines with DNA-PKcs to suppress gastric cancer metastasis.
Article Snippet: Long non-coding RNAs (lncRNAs) play important roles in cancer development and progression; however, their contributions to gastric cancer metastasis remain largely unknown.. By lncRNA microarray screening, our study showed that 453 lncRNAs are dysregulated in gastric cancer tissues with or without lymph node metastasis, of which lnc-LEMGC ranks as one of the most significantly downregulated lncRNAs.. Lnc-LEMGC inhibited cell migration and invasion both in vitro and in vivo, by combining with protein DNA-PKcs.

Article Title: PIN1P1 is activated by CREB1 and promotes gastric cancer progression via interacting with YBX1 and upregulating PIN1.
Article Snippet: .. Differential lncRNA screening was performed using the Human Gene Expression Microarray (Human 8 × 60 K LncRNA Microarray v2.0; Arraystar). ..

Microarray:

Article Title: KIF26B, a novel oncogene, promotes proliferation and metastasis by activating the VEGF pathway in gastric cancer.
Article Snippet: Tumor metastasis is the main reason of cancer-related death for gastric cancer (GC) patients and gene expression microarray data indicate that kinesin family member 26B (KIF26B) is one of the most upregulated genes in metastatic GC samples.. Specifically, KIF26B expression was upregulated in a stepwise manner from non-tumorous gastric mucosa, primary GC tissues without metastasis, via primary GC tissues with metastasis, to secondary lymph node metastatic (LNM) foci.. Increased expression of KIF26B was correlated with tumor size, positive LNM or distant metastases and poor prognosis.

Article Title: SP-1-activated LINC01016 overexpression promotes gastric cancer invasion and metastasis through inhibiting EIF4A3-mediated MMP9 mRNA decay
Article Snippet: .. First, differential gene expression analysis of lncRNAs was performed between five cases of gastric cancers with LNM and another five cases without LNM using the Human Gene Expression Microarray (Human 8×60 K LncRNA Microarray v2.0; Arraystar, Rockville, USA). ..

Article Title: SP-1-activated LINC01016 overexpression promotes gastric cancer invasion and metastasis through inhibiting EIF4A3-mediated MMP9 mRNA decay.
Article Snippet: .. RESULTS LINC01016 is upregulated in GC with lymph node metastasis First, differential gene expression analysis of lncRNAs was performed between five cases of gastric cancers with LNM and another five cases without LNM using the Human Gene Expression Microarray (Human 8×60 K LncRNA Microarray v2.0; Arraystar, Rockville, USA). ..

Article Title: Long noncoding RNA lnc-LEMGC combines with DNA-PKcs to suppress gastric cancer metastasis.
Article Snippet: Long non-coding RNAs (lncRNAs) play important roles in cancer development and progression; however, their contributions to gastric cancer metastasis remain largely unknown.. By lncRNA microarray screening, our study showed that 453 lncRNAs are dysregulated in gastric cancer tissues with or without lymph node metastasis, of which lnc-LEMGC ranks as one of the most significantly downregulated lncRNAs.. Lnc-LEMGC inhibited cell migration and invasion both in vitro and in vivo, by combining with protein DNA-PKcs.

Article Title: PIN1P1 is activated by CREB1 and promotes gastric cancer progression via interacting with YBX1 and upregulating PIN1.
Article Snippet: .. Differential lncRNA screening was performed using the Human Gene Expression Microarray (Human 8 × 60 K LncRNA Microarray v2.0; Arraystar). ..

Transfection:

Article Title: LncRNA PCAT1 and its genetic variant rs1902432 are associated with prostate cancer risk
Article Snippet: Total RNA was extracted from DU145 PCa cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. .. The mRNA of three pairs of DU145 cells transfected with PCAT1 overexpression vector or corresponding NC vector was profiled with Arraystar Human Gene Expression Array. ..

Article Title: LncRNA PCAT1 and its genetic variant rs1902432 are associated with prostate cancer risk
Article Snippet: .. Arraystar Human Gene Expression Array was conducted to assess the genome-wide profiling of gene expression from PCa cell DU145 transfected with PCAT1 overexpression vector or NC vector. ..

Over Expression:

Article Title: LncRNA PCAT1 and its genetic variant rs1902432 are associated with prostate cancer risk
Article Snippet: Total RNA was extracted from DU145 PCa cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. .. The mRNA of three pairs of DU145 cells transfected with PCAT1 overexpression vector or corresponding NC vector was profiled with Arraystar Human Gene Expression Array. ..

Article Title: LncRNA PCAT1 and its genetic variant rs1902432 are associated with prostate cancer risk
Article Snippet: .. Arraystar Human Gene Expression Array was conducted to assess the genome-wide profiling of gene expression from PCa cell DU145 transfected with PCAT1 overexpression vector or NC vector. ..

Plasmid Preparation:

Article Title: LncRNA PCAT1 and its genetic variant rs1902432 are associated with prostate cancer risk
Article Snippet: Total RNA was extracted from DU145 PCa cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. .. The mRNA of three pairs of DU145 cells transfected with PCAT1 overexpression vector or corresponding NC vector was profiled with Arraystar Human Gene Expression Array. ..

Article Title: LncRNA PCAT1 and its genetic variant rs1902432 are associated with prostate cancer risk
Article Snippet: .. Arraystar Human Gene Expression Array was conducted to assess the genome-wide profiling of gene expression from PCa cell DU145 transfected with PCAT1 overexpression vector or NC vector. ..

Genome Wide:

Article Title: LncRNA PCAT1 and its genetic variant rs1902432 are associated with prostate cancer risk
Article Snippet: .. Arraystar Human Gene Expression Array was conducted to assess the genome-wide profiling of gene expression from PCa cell DU145 transfected with PCAT1 overexpression vector or NC vector. ..



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ZEB1-AS1 sponges miR-141-3p in CRC cells. ( A ) Nuclear and cytoplasmic fractionation was analyzed for ZEB1-AS1 expression in SW480 and LOVO. ( B ) The microRNA array analysis in normal and tumor tissues. ( C ) The potential binding sites between ZEB1-AS1 and miR-141-3p. ( D ) The expressions of miR-141-3p in CRC tissues were detected by RT-qPCR. ( E ) Luciferase reporter assay showed ZEB1-AS1-wt activity was impaired by miR-141-3p. ( F ) The expression of miR-141-3p in SW480 and LOVO was upregulated after ZEB1-AS1 expression was downregulated identified by RT-qPCR. ( G ) The expression of miR-141-3p was negatively correlated with ZEB1-AS1 expression in CRC tissues. * P < 0.05.

Journal: International Journal of Medical Sciences

Article Title: Long noncoding RNA ZEB1-AS1 acts as a Sponge of miR-141-3p to Inhibit Cell Proliferation in Colorectal Cancer

doi: 10.7150/ijms.46698

Figure Lengend Snippet: ZEB1-AS1 sponges miR-141-3p in CRC cells. ( A ) Nuclear and cytoplasmic fractionation was analyzed for ZEB1-AS1 expression in SW480 and LOVO. ( B ) The microRNA array analysis in normal and tumor tissues. ( C ) The potential binding sites between ZEB1-AS1 and miR-141-3p. ( D ) The expressions of miR-141-3p in CRC tissues were detected by RT-qPCR. ( E ) Luciferase reporter assay showed ZEB1-AS1-wt activity was impaired by miR-141-3p. ( F ) The expression of miR-141-3p in SW480 and LOVO was upregulated after ZEB1-AS1 expression was downregulated identified by RT-qPCR. ( G ) The expression of miR-141-3p was negatively correlated with ZEB1-AS1 expression in CRC tissues. * P < 0.05.

Article Snippet: Shanghai Biotechnology Co., Ltd conducted the microRNA microarray gene expression experiments and data analysis.

Techniques: Fractionation, Expressing, Binding Assay, Quantitative RT-PCR, Luciferase, Reporter Assay, Activity Assay

ABCG2 upregulation is associated with SIK3 attenuation. (A) List of ABC family proteins from microarray data for which the expression changed more than 1.5-fold compared with control cells. (B) The mRNA expression levels of ABC proteins were verified by real-time PCR in OVCAR4 and SKOV3 cells. The white bar (shLuc) represents control cells, and the gray bar (shSIK3#01) and black bar (shSIK3#61) represent indicated cells with SIK3 knockdown. Data are represented as the mean ± SEM from three independent experiments and analyzed by one-way ANOVA. *: p <0.05 and **: p <0.01 (compared to the shLuc control). Note that ABCG1 and ABCG2 were both significantly upregulated in cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (C) The protein expression levels of ABCG1 and ABCG2 were further verified by Western blotting. Note that only ABCG2 was upregulated in OVCAR4 and SKOV3 cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (D) The functional MDR activity of the ABCG2 protein was determined using an MDR assay kit (Abcam). A total of 2 x 10 5 suspended cells were pretreated with the inhibitor novobiocin (50 nM) or DMSO. Then, diluted Efflux Gold Detection Reagent was added at 37°C for 30 minutes. The cellular orange fluorescence signal of the Efflux Gold Detection Reagent was measured immediately by flow cytometry in the living (PI-negative) cell population. The numbers in the upper left corners are MAF values.

Journal: Journal of Cancer

Article Title: Downregulated Salt-inducible Kinase 3 Expression Promotes Chemoresistance in Serous Ovarian Cancer via the ATP‐binding Cassette Protein ABCG2

doi: 10.7150/jca.34886

Figure Lengend Snippet: ABCG2 upregulation is associated with SIK3 attenuation. (A) List of ABC family proteins from microarray data for which the expression changed more than 1.5-fold compared with control cells. (B) The mRNA expression levels of ABC proteins were verified by real-time PCR in OVCAR4 and SKOV3 cells. The white bar (shLuc) represents control cells, and the gray bar (shSIK3#01) and black bar (shSIK3#61) represent indicated cells with SIK3 knockdown. Data are represented as the mean ± SEM from three independent experiments and analyzed by one-way ANOVA. *: p <0.05 and **: p <0.01 (compared to the shLuc control). Note that ABCG1 and ABCG2 were both significantly upregulated in cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (C) The protein expression levels of ABCG1 and ABCG2 were further verified by Western blotting. Note that only ABCG2 was upregulated in OVCAR4 and SKOV3 cells with SIK3 knocked down by shSIK3#01 and shSIK3#61. (D) The functional MDR activity of the ABCG2 protein was determined using an MDR assay kit (Abcam). A total of 2 x 10 5 suspended cells were pretreated with the inhibitor novobiocin (50 nM) or DMSO. Then, diluted Efflux Gold Detection Reagent was added at 37°C for 30 minutes. The cellular orange fluorescence signal of the Efflux Gold Detection Reagent was measured immediately by flow cytometry in the living (PI-negative) cell population. The numbers in the upper left corners are MAF values.

Article Snippet: TRIzol-isolated RNA samples were shipped on dry ice to Welgene Biotech (Taiwan), where the gene expression microarray experiments were performed as a contract service.

Techniques: Microarray, Expressing, Control, Real-time Polymerase Chain Reaction, Knockdown, Western Blot, Functional Assay, Activity Assay, Fluorescence, Flow Cytometry