e-gel gelquant express analysis software (version 1.14.6.0 (dongle) (Thermo Fisher)
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E Gel Gelquant Express Analysis Software (Version 1.14.6.0 (Dongle), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Polymerase Chain Reaction:Article Title: Opposite effects of dopamine on the mechanical activity of circular and longitudinal muscle of human colon. Article Snippet: The idea of a dopaminergic enteric transmission, independent of the sympathoadrenal system, has been proposed different years ago due to the observations that neurons, expressing both the dopamine (DA)-synthesizing enzyme and the dopamine transporter, but not the norepinephrine-synthesizing enzyme, were present within the enteric nervous system (ENS) in both animal models and humans.1,2 DA seems to be implicated in several functions in GI tract,3 activating specific receptors belonging to the G protein–coupled receptor family categorized in two main families: D1-like receptor (D1 and D5 receptors) and D2-like receptor(D2, D3, and D4 receptors), present from the stomach through the distal colon in different animal species.2 Received: 17 August 2019 | Revised: 8 January 2020 | Accepted: 14 January 2020 DOI: 10.1111/nmo.13811 Article Title: Pistachio Consumption Alleviates Inflammation and Improves Gut Microbiota Composition in Mice Fed a High-Fat Diet Article Snippet: The expression levels of the gene targets, normalized to the endogenous reference (β-actin), were analyzed using the Article Title: Reduced Expression of VEGF-A in Human Retinal Pigment Epithelial Cells and Human Muller Cells Following CRISPR-Cas9 Ribonucleoprotein-Mediated Gene Disruption Article Snippet: The genomic cleavage efficiency was quantified using E-Gel GelQuant Express Analysis Software ( Article Title: Long-Term Ingestion of Sicilian Black Bee Chestnut Honey and/or D-Limonene Counteracts Brain Damage Induced by High Fat-Diet in Obese Mice Article Snippet: The quantification of gene expression was obtained by using Article Title: Indicaxanthin from Opuntia ficus-indica Fruit Ameliorates Glucose Dysmetabolism and Counteracts Insulin Resistance in High-Fat-Diet-Fed Mice. Article Snippet: The signal intensity of the products was analysed using Article Title: Positive Impacts of Aphanizomenon Flos Aquae Extract on Obesity-Related Dysmetabolism in Mice with Diet-Induced Obesity Article Snippet: Intensities of the PCR bands were analysed densitometrically using Software:Article Title: Opposite effects of dopamine on the mechanical activity of circular and longitudinal muscle of human colon. Article Snippet: The idea of a dopaminergic enteric transmission, independent of the sympathoadrenal system, has been proposed different years ago due to the observations that neurons, expressing both the dopamine (DA)-synthesizing enzyme and the dopamine transporter, but not the norepinephrine-synthesizing enzyme, were present within the enteric nervous system (ENS) in both animal models and humans.1,2 DA seems to be implicated in several functions in GI tract,3 activating specific receptors belonging to the G protein–coupled receptor family categorized in two main families: D1-like receptor (D1 and D5 receptors) and D2-like receptor(D2, D3, and D4 receptors), present from the stomach through the distal colon in different animal species.2 Received: 17 August 2019 | Revised: 8 January 2020 | Accepted: 14 January 2020 DOI: 10.1111/nmo.13811 Article Title: Pistachio Consumption Alleviates Inflammation and Improves Gut Microbiota Composition in Mice Fed a High-Fat Diet Article Snippet: The expression levels of the gene targets, normalized to the endogenous reference (β-actin), were analyzed using the Article Title: Reduced Expression of VEGF-A in Human Retinal Pigment Epithelial Cells and Human Muller Cells Following CRISPR-Cas9 Ribonucleoprotein-Mediated Gene Disruption Article Snippet: The genomic cleavage efficiency was quantified using E-Gel GelQuant Express Analysis Software ( Article Title: Long-Term Ingestion of Sicilian Black Bee Chestnut Honey and/or D-Limonene Counteracts Brain Damage Induced by High Fat-Diet in Obese Mice Article Snippet: The quantification of gene expression was obtained by using Article Title: Indicaxanthin from Opuntia ficus-indica Fruit Ameliorates Glucose Dysmetabolism and Counteracts Insulin Resistance in High-Fat-Diet-Fed Mice. Article Snippet: The signal intensity of the products was analysed using Article Title: Positive Impacts of Aphanizomenon Flos Aquae Extract on Obesity-Related Dysmetabolism in Mice with Diet-Induced Obesity Article Snippet: Intensities of the PCR bands were analysed densitometrically using Gene Expression:Article Title: Opposite effects of dopamine on the mechanical activity of circular and longitudinal muscle of human colon. Article Snippet: The idea of a dopaminergic enteric transmission, independent of the sympathoadrenal system, has been proposed different years ago due to the observations that neurons, expressing both the dopamine (DA)-synthesizing enzyme and the dopamine transporter, but not the norepinephrine-synthesizing enzyme, were present within the enteric nervous system (ENS) in both animal models and humans.1,2 DA seems to be implicated in several functions in GI tract,3 activating specific receptors belonging to the G protein–coupled receptor family categorized in two main families: D1-like receptor (D1 and D5 receptors) and D2-like receptor(D2, D3, and D4 receptors), present from the stomach through the distal colon in different animal species.2 Received: 17 August 2019 | Revised: 8 January 2020 | Accepted: 14 January 2020 DOI: 10.1111/nmo.13811 Article Title: Pistachio Consumption Alleviates Inflammation and Improves Gut Microbiota Composition in Mice Fed a High-Fat Diet Article Snippet: The expression levels of the gene targets, normalized to the endogenous reference (β-actin), were analyzed using the Article Title: Reduced Expression of VEGF-A in Human Retinal Pigment Epithelial Cells and Human Muller Cells Following CRISPR-Cas9 Ribonucleoprotein-Mediated Gene Disruption Article Snippet: The genomic cleavage efficiency was quantified using E-Gel GelQuant Express Analysis Software ( Article Title: Long-Term Ingestion of Sicilian Black Bee Chestnut Honey and/or D-Limonene Counteracts Brain Damage Induced by High Fat-Diet in Obese Mice Article Snippet: The quantification of gene expression was obtained by using Article Title: Indicaxanthin from Opuntia ficus-indica Fruit Ameliorates Glucose Dysmetabolism and Counteracts Insulin Resistance in High-Fat-Diet-Fed Mice. Article Snippet: The signal intensity of the products was analysed using Article Title: Positive Impacts of Aphanizomenon Flos Aquae Extract on Obesity-Related Dysmetabolism in Mice with Diet-Induced Obesity Article Snippet: Intensities of the PCR bands were analysed densitometrically using Expressing:Article Title: Opposite effects of dopamine on the mechanical activity of circular and longitudinal muscle of human colon. Article Snippet: The idea of a dopaminergic enteric transmission, independent of the sympathoadrenal system, has been proposed different years ago due to the observations that neurons, expressing both the dopamine (DA)-synthesizing enzyme and the dopamine transporter, but not the norepinephrine-synthesizing enzyme, were present within the enteric nervous system (ENS) in both animal models and humans.1,2 DA seems to be implicated in several functions in GI tract,3 activating specific receptors belonging to the G protein–coupled receptor family categorized in two main families: D1-like receptor (D1 and D5 receptors) and D2-like receptor(D2, D3, and D4 receptors), present from the stomach through the distal colon in different animal species.2 Received: 17 August 2019 | Revised: 8 January 2020 | Accepted: 14 January 2020 DOI: 10.1111/nmo.13811 Article Title: Pistachio Consumption Alleviates Inflammation and Improves Gut Microbiota Composition in Mice Fed a High-Fat Diet Article Snippet: The expression levels of the gene targets, normalized to the endogenous reference (β-actin), were analyzed using the Article Title: Reduced Expression of VEGF-A in Human Retinal Pigment Epithelial Cells and Human Muller Cells Following CRISPR-Cas9 Ribonucleoprotein-Mediated Gene Disruption Article Snippet: The genomic cleavage efficiency was quantified using E-Gel GelQuant Express Analysis Software ( Article Title: Long-Term Ingestion of Sicilian Black Bee Chestnut Honey and/or D-Limonene Counteracts Brain Damage Induced by High Fat-Diet in Obese Mice Article Snippet: The quantification of gene expression was obtained by using Article Title: Indicaxanthin from Opuntia ficus-indica Fruit Ameliorates Glucose Dysmetabolism and Counteracts Insulin Resistance in High-Fat-Diet-Fed Mice. Article Snippet: The signal intensity of the products was analysed using Article Title: Positive Impacts of Aphanizomenon Flos Aquae Extract on Obesity-Related Dysmetabolism in Mice with Diet-Induced Obesity Article Snippet: Intensities of the PCR bands were analysed densitometrically using Electrophoresis:Article Title: Opposite effects of dopamine on the mechanical activity of circular and longitudinal muscle of human colon. Article Snippet: The idea of a dopaminergic enteric transmission, independent of the sympathoadrenal system, has been proposed different years ago due to the observations that neurons, expressing both the dopamine (DA)-synthesizing enzyme and the dopamine transporter, but not the norepinephrine-synthesizing enzyme, were present within the enteric nervous system (ENS) in both animal models and humans.1,2 DA seems to be implicated in several functions in GI tract,3 activating specific receptors belonging to the G protein–coupled receptor family categorized in two main families: D1-like receptor (D1 and D5 receptors) and D2-like receptor(D2, D3, and D4 receptors), present from the stomach through the distal colon in different animal species.2 Received: 17 August 2019 | Revised: 8 January 2020 | Accepted: 14 January 2020 DOI: 10.1111/nmo.13811 Article Title: Pistachio Consumption Alleviates Inflammation and Improves Gut Microbiota Composition in Mice Fed a High-Fat Diet Article Snippet: The expression levels of the gene targets, normalized to the endogenous reference (β-actin), were analyzed using the Article Title: Reduced Expression of VEGF-A in Human Retinal Pigment Epithelial Cells and Human Muller Cells Following CRISPR-Cas9 Ribonucleoprotein-Mediated Gene Disruption Article Snippet: The genomic cleavage efficiency was quantified using E-Gel GelQuant Express Analysis Software ( Article Title: Long-Term Ingestion of Sicilian Black Bee Chestnut Honey and/or D-Limonene Counteracts Brain Damage Induced by High Fat-Diet in Obese Mice Article Snippet: The quantification of gene expression was obtained by using Article Title: Indicaxanthin from Opuntia ficus-indica Fruit Ameliorates Glucose Dysmetabolism and Counteracts Insulin Resistance in High-Fat-Diet-Fed Mice. Article Snippet: The signal intensity of the products was analysed using Article Title: Positive Impacts of Aphanizomenon Flos Aquae Extract on Obesity-Related Dysmetabolism in Mice with Diet-Induced Obesity Article Snippet: Intensities of the PCR bands were analysed densitometrically using Agarose Gel Electrophoresis:Article Title: Opposite effects of dopamine on the mechanical activity of circular and longitudinal muscle of human colon. Article Snippet: The idea of a dopaminergic enteric transmission, independent of the sympathoadrenal system, has been proposed different years ago due to the observations that neurons, expressing both the dopamine (DA)-synthesizing enzyme and the dopamine transporter, but not the norepinephrine-synthesizing enzyme, were present within the enteric nervous system (ENS) in both animal models and humans.1,2 DA seems to be implicated in several functions in GI tract,3 activating specific receptors belonging to the G protein–coupled receptor family categorized in two main families: D1-like receptor (D1 and D5 receptors) and D2-like receptor(D2, D3, and D4 receptors), present from the stomach through the distal colon in different animal species.2 Received: 17 August 2019 | Revised: 8 January 2020 | Accepted: 14 January 2020 DOI: 10.1111/nmo.13811 Article Title: Pistachio Consumption Alleviates Inflammation and Improves Gut Microbiota Composition in Mice Fed a High-Fat Diet Article Snippet: The expression levels of the gene targets, normalized to the endogenous reference (β-actin), were analyzed using the Article Title: Reduced Expression of VEGF-A in Human Retinal Pigment Epithelial Cells and Human Muller Cells Following CRISPR-Cas9 Ribonucleoprotein-Mediated Gene Disruption Article Snippet: The genomic cleavage efficiency was quantified using E-Gel GelQuant Express Analysis Software ( Article Title: Long-Term Ingestion of Sicilian Black Bee Chestnut Honey and/or D-Limonene Counteracts Brain Damage Induced by High Fat-Diet in Obese Mice Article Snippet: The quantification of gene expression was obtained by using Article Title: Indicaxanthin from Opuntia ficus-indica Fruit Ameliorates Glucose Dysmetabolism and Counteracts Insulin Resistance in High-Fat-Diet-Fed Mice. Article Snippet: The signal intensity of the products was analysed using Article Title: Positive Impacts of Aphanizomenon Flos Aquae Extract on Obesity-Related Dysmetabolism in Mice with Diet-Induced Obesity Article Snippet: Intensities of the PCR bands were analysed densitometrically using Staining:Article Title: Opposite effects of dopamine on the mechanical activity of circular and longitudinal muscle of human colon. Article Snippet: The idea of a dopaminergic enteric transmission, independent of the sympathoadrenal system, has been proposed different years ago due to the observations that neurons, expressing both the dopamine (DA)-synthesizing enzyme and the dopamine transporter, but not the norepinephrine-synthesizing enzyme, were present within the enteric nervous system (ENS) in both animal models and humans.1,2 DA seems to be implicated in several functions in GI tract,3 activating specific receptors belonging to the G protein–coupled receptor family categorized in two main families: D1-like receptor (D1 and D5 receptors) and D2-like receptor(D2, D3, and D4 receptors), present from the stomach through the distal colon in different animal species.2 Received: 17 August 2019 | Revised: 8 January 2020 | Accepted: 14 January 2020 DOI: 10.1111/nmo.13811 Article Title: Pistachio Consumption Alleviates Inflammation and Improves Gut Microbiota Composition in Mice Fed a High-Fat Diet Article Snippet: The expression levels of the gene targets, normalized to the endogenous reference (β-actin), were analyzed using the Article Title: Reduced Expression of VEGF-A in Human Retinal Pigment Epithelial Cells and Human Muller Cells Following CRISPR-Cas9 Ribonucleoprotein-Mediated Gene Disruption Article Snippet: The genomic cleavage efficiency was quantified using E-Gel GelQuant Express Analysis Software ( Article Title: Long-Term Ingestion of Sicilian Black Bee Chestnut Honey and/or D-Limonene Counteracts Brain Damage Induced by High Fat-Diet in Obese Mice Article Snippet: The quantification of gene expression was obtained by using Article Title: Indicaxanthin from Opuntia ficus-indica Fruit Ameliorates Glucose Dysmetabolism and Counteracts Insulin Resistance in High-Fat-Diet-Fed Mice. Article Snippet: The signal intensity of the products was analysed using Article Title: Positive Impacts of Aphanizomenon Flos Aquae Extract on Obesity-Related Dysmetabolism in Mice with Diet-Induced Obesity Article Snippet: Intensities of the PCR bands were analysed densitometrically using |

![Yield of the amplicons using templates with different quality for all master mixes kits tested. In yellow the quantification in nanograms (ng) in the agarose gel made by <t>GelQuant</t> Express—Image Analysis software. Lanes: 1 to 3—Invitrogen™ PCR Supermix Brasil (Thermo Fisher Scientific); 4 to 6—Platinum® Multiplex PCR Master Mix (Thermo Fisher Scientific); 7 to 9—2 × PCR SuperMix (Bio-Helix); 10 to 12—QIAGEN® Multiplex PCR Kit (Qiagen); 13 to 15—GoTaq® Hot Start Green Master Mix (Promega); 16—molecular weight. Templates features: A1—Purified DNA from a serotype 22F strain; A2—DNA extracted from CSF sample with high quality and identified as the presence of serotype 8 strain; A3—DNA extracted from CSF sample with low quality and identified as the presence of serotype 22F strain (same CSF sample from which the strain presented in lanes A1 was isolated). The arrow indicates the 160 bp cpsA gene; The dotted arrow indicates unspecific bands. The table below the figure represents: [ ] ng/µL—concentration in ng of the amplified band correspondent to the genotyping, excluding the band referring to the cpsA gene; total ng—the total amount of produced DNA in ng contained in the total reaction volume correspondent only to the genotyping band](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4411/pmc09854411/pmc09854411__10096_2023_4547_Fig2_HTML.jpg)