e gel gelquant express analysis software (Thermo Fisher)
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E Gel Gelquant Express Analysis Software, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Software:Article Title: Indicaxanthin from Opuntia ficus-indica Fruit Ameliorates Glucose Dysmetabolism and Counteracts Insulin Resistance in High-Fat-Diet-Fed Mice Article Snippet: After 40 cycles, the PCR products were separated on a 1.8% agarose gel and visualised by ultraviolet (UV) light using E-Gel GelCapture (Thermo Fisher Scientific, Monza, Italy). .. The signal intensity of the products was analysed using Article Title: Pistachio Consumption Prevents and Improves Lipid Dysmetabolism by Reducing the Lipid Metabolizing Gene Expression in Diet-Induced Obese Mice Article Snippet: .. The amplification cycles included denaturation at 95 °C for 10 s, annealing at 60 °C for 15 s, and elongation at 72 °C for 15 s. After 35 cycles, the PCR products were separated by electrophoresis on a 1.8% agarose gel for 45 min at 85 V. The gels were stained with 1 mg/mL ethidium bromide and visualized with ultraviolet (UV) light using E-Gel GelCapture (Thermo Fisher Scientific, Monza, Italy), and the expression levels of the gene targets, normalized to the endogenous reference (β-actin), were analyzed using Expressing:Article Title: Indicaxanthin from Opuntia ficus-indica Fruit Ameliorates Glucose Dysmetabolism and Counteracts Insulin Resistance in High-Fat-Diet-Fed Mice Article Snippet: After 40 cycles, the PCR products were separated on a 1.8% agarose gel and visualised by ultraviolet (UV) light using E-Gel GelCapture (Thermo Fisher Scientific, Monza, Italy). .. The signal intensity of the products was analysed using Article Title: Pistachio Consumption Prevents and Improves Lipid Dysmetabolism by Reducing the Lipid Metabolizing Gene Expression in Diet-Induced Obese Mice Article Snippet: .. The amplification cycles included denaturation at 95 °C for 10 s, annealing at 60 °C for 15 s, and elongation at 72 °C for 15 s. After 35 cycles, the PCR products were separated by electrophoresis on a 1.8% agarose gel for 45 min at 85 V. The gels were stained with 1 mg/mL ethidium bromide and visualized with ultraviolet (UV) light using E-Gel GelCapture (Thermo Fisher Scientific, Monza, Italy), and the expression levels of the gene targets, normalized to the endogenous reference (β-actin), were analyzed using Amplification:Article Title: Pistachio Consumption Prevents and Improves Lipid Dysmetabolism by Reducing the Lipid Metabolizing Gene Expression in Diet-Induced Obese Mice Article Snippet: .. The amplification cycles included denaturation at 95 °C for 10 s, annealing at 60 °C for 15 s, and elongation at 72 °C for 15 s. After 35 cycles, the PCR products were separated by electrophoresis on a 1.8% agarose gel for 45 min at 85 V. The gels were stained with 1 mg/mL ethidium bromide and visualized with ultraviolet (UV) light using E-Gel GelCapture (Thermo Fisher Scientific, Monza, Italy), and the expression levels of the gene targets, normalized to the endogenous reference (β-actin), were analyzed using Polymerase Chain Reaction:Article Title: Pistachio Consumption Prevents and Improves Lipid Dysmetabolism by Reducing the Lipid Metabolizing Gene Expression in Diet-Induced Obese Mice Article Snippet: .. The amplification cycles included denaturation at 95 °C for 10 s, annealing at 60 °C for 15 s, and elongation at 72 °C for 15 s. After 35 cycles, the PCR products were separated by electrophoresis on a 1.8% agarose gel for 45 min at 85 V. The gels were stained with 1 mg/mL ethidium bromide and visualized with ultraviolet (UV) light using E-Gel GelCapture (Thermo Fisher Scientific, Monza, Italy), and the expression levels of the gene targets, normalized to the endogenous reference (β-actin), were analyzed using Electrophoresis:Article Title: Pistachio Consumption Prevents and Improves Lipid Dysmetabolism by Reducing the Lipid Metabolizing Gene Expression in Diet-Induced Obese Mice Article Snippet: .. The amplification cycles included denaturation at 95 °C for 10 s, annealing at 60 °C for 15 s, and elongation at 72 °C for 15 s. After 35 cycles, the PCR products were separated by electrophoresis on a 1.8% agarose gel for 45 min at 85 V. The gels were stained with 1 mg/mL ethidium bromide and visualized with ultraviolet (UV) light using E-Gel GelCapture (Thermo Fisher Scientific, Monza, Italy), and the expression levels of the gene targets, normalized to the endogenous reference (β-actin), were analyzed using Agarose Gel Electrophoresis:Article Title: Pistachio Consumption Prevents and Improves Lipid Dysmetabolism by Reducing the Lipid Metabolizing Gene Expression in Diet-Induced Obese Mice Article Snippet: .. The amplification cycles included denaturation at 95 °C for 10 s, annealing at 60 °C for 15 s, and elongation at 72 °C for 15 s. After 35 cycles, the PCR products were separated by electrophoresis on a 1.8% agarose gel for 45 min at 85 V. The gels were stained with 1 mg/mL ethidium bromide and visualized with ultraviolet (UV) light using E-Gel GelCapture (Thermo Fisher Scientific, Monza, Italy), and the expression levels of the gene targets, normalized to the endogenous reference (β-actin), were analyzed using Staining:Article Title: Pistachio Consumption Prevents and Improves Lipid Dysmetabolism by Reducing the Lipid Metabolizing Gene Expression in Diet-Induced Obese Mice Article Snippet: .. The amplification cycles included denaturation at 95 °C for 10 s, annealing at 60 °C for 15 s, and elongation at 72 °C for 15 s. After 35 cycles, the PCR products were separated by electrophoresis on a 1.8% agarose gel for 45 min at 85 V. The gels were stained with 1 mg/mL ethidium bromide and visualized with ultraviolet (UV) light using E-Gel GelCapture (Thermo Fisher Scientific, Monza, Italy), and the expression levels of the gene targets, normalized to the endogenous reference (β-actin), were analyzed using |

![Yield of the amplicons using templates with different quality for all master mixes kits tested. In yellow the quantification in nanograms (ng) in the agarose gel made by <t>GelQuant</t> Express—Image Analysis software. Lanes: 1 to 3—Invitrogen™ PCR Supermix Brasil (Thermo Fisher Scientific); 4 to 6—Platinum® Multiplex PCR Master Mix (Thermo Fisher Scientific); 7 to 9—2 × PCR SuperMix (Bio-Helix); 10 to 12—QIAGEN® Multiplex PCR Kit (Qiagen); 13 to 15—GoTaq® Hot Start Green Master Mix (Promega); 16—molecular weight. Templates features: A1—Purified DNA from a serotype 22F strain; A2—DNA extracted from CSF sample with high quality and identified as the presence of serotype 8 strain; A3—DNA extracted from CSF sample with low quality and identified as the presence of serotype 22F strain (same CSF sample from which the strain presented in lanes A1 was isolated). The arrow indicates the 160 bp cpsA gene; The dotted arrow indicates unspecific bands. The table below the figure represents: [ ] ng/µL—concentration in ng of the amplified band correspondent to the genotyping, excluding the band referring to the cpsA gene; total ng—the total amount of produced DNA in ng contained in the total reaction volume correspondent only to the genotyping band](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4411/pmc09854411/pmc09854411__10096_2023_4547_Fig2_HTML.jpg)