nebnextr rna fragmentation module (New England Biolabs)

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New England Biolabs nebnextr rna fragmentation module
Nebnextr Rna Fragmentation Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 997 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fragmentation+module/NEBNext+Magnesium+RNA+Fragmentation+Module/ppr0926076-82-10-14
Average 99 stars, based on 997 article reviews

nebnextr rna fragmentation module - by Bioz Stars, 2026-10

99/100 stars

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Purification:

Article Title: LIMCH1 inhibits antitumor immunity by upregulating PDL1 in triple-negative breast cancer
Article Snippet: .. Poly(A) RNA was purified from 1 μg total RNA using Dynabeads Oligo(dT)25 (Thermo Fisher, USA) in two rounds and fragmented with Magnesium RNA Fragmentation Module (NEB, USA). .. The fragments were reverse-transcribed into cDNA using SuperScriptTM II (Invitrogen, USA), followed by synthesis of U-labeled second-strand DNA.

Article Title: Integrated multi-tissue transcriptomics reveals cross-tissue regulatory networks and hub genes regulating feed efficiency in aging chicken
Article Snippet: After total RNA was extracted, mRNA was purified from total RNA (5ug) using Dynabeads Oligo (dT) (Thermo Fisher, CA, USA) with two rounds of purification. .. Following purification, the mRNA was fragmented into short fragments using divalent cations under elevated temperature (Magnesium RNA Fragmentation Module (NEB, cat.e6150, USA) under 94°C 5-7 min). .. Then the cleaved RNA fragments were reverse-transcribed to create the cDNA by SuperScriptTM II Reverse Transcriptase (Invitrogen, cat. 1896649, USA), which were next used to synthesise U-labeled second-stranded DNAs with E. coli DNA polymerase I (NEB, cat.m0209, USA), RNase H (NEB, cat.m0297, USA) and dUTP Solution (Thermo Fisher, cat.R0133, USA).

other:

Article Title: HDAC2-mediated recruitment of METTL3 to chromatin regulates human embryonic stem cell differentiation.
Article Snippet: The chromatin was collected and fragmented using Diagenode Biorupter Pico for 10 cycles with 20s-on/30s-off.

cDNA Synthesis:

Article Title: Targeted metabolomic and transcriptomic reveal the regulatory network of ultrasound on polyphenol biosynthesis in tender coconut flesh during storage
Article Snippet: Then, Polyadenylated mRNA was isolated and purified using Dynabeads Oligo (dT) (Thermo Fisher Scientific, Waltham, USA). .. Subsequently, the mRNA fragments were performed with Magnesium RNA Fragmentation Module (NEBNext, Ipswich, USA) under high temperature conditions, and then followed by first-strand cDNA synthesis using SuperScriptTM II Reverse Transcriptase (Invitrogen, Waltham, USA). .. E.coli DNA polymerase I (NEB, Ipswich, USA) and RNase H (NEB, Ipswich, USA) were used to synthesize Second-stranded DNA.

Reverse Transcription:

Article Title: Epigenetic reprogramming via EZH2 inhibition rescues fibroadipose pathogenesis in secondary lymphedema through activating PPARγ signaling
Article Snippet: Two rounds of purification of Poly (A) RNA were carried out from 1 μg total RNA using Dynabeads Oligo(dT) 25 (Thermo Fisher, 61005, CA, USA). .. Poly(A) RNA was then fragmented into small pieces using Magnesium RNA Fragmentation Module (NEB, E6150, USA) under 94 °C for 5–7 min. Cleaved RNA fragments were reverse-transcribed to cDNA by using SuperScriptTM II Reverse Transcriptase (Invitrogen, 1896649), which were next used to synthesize U-labeled second-stranded DNAs with E. coli DNA polymerase I (NEB, M0209, USA), RNase H (NEB, M0297, USA) and dUTP Solution (Thermo Fisher, R0133, USA). ..

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