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klenow fragment  (New England Biolabs)


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    Structured Review

    New England Biolabs klenow fragment
    Klenow Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 495 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fragmentation+module/NEBNext+dATailing+Module/pmc13112749-375-26-28
    Average 97 stars, based on 495 article reviews
    klenow fragment - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Ligation:

    Article Title: Genome-wide maps of CPD deamination in yeast reveal the impact of DNA sequence context and nucleosome architecture on cytosine deamination rates
    Article Snippet: Ligated DNA was then sonicated (Diagenode Bioruptor 300) to achieve DNA fragments ∼200–400 bp in length, and captured with streptavidin beads (Invitrogen), and washed with 1× saline sodium citrate (SSC) buffer to remove nonspecific binding, and nonbiotinylated strand containing the ligated 3′ adaptor was eluted with 0.15 m NaOH. .. After synthesis of the complementary strand with EconoTaq (Lucigen) and preparation of DNA ends with NEBNext End Repair and NEBNext dA-Tailing modules, a second adaptor ligation was performed followed by eight to 10 cycles of PCR amplification. .. Libraries were submitted for either Ion Torrent (WSU LBB1 Core Facility) or Illumina (Azenta) sequencing.

    Polymerase Chain Reaction:

    Article Title: Genome-wide maps of CPD deamination in yeast reveal the impact of DNA sequence context and nucleosome architecture on cytosine deamination rates
    Article Snippet: Ligated DNA was then sonicated (Diagenode Bioruptor 300) to achieve DNA fragments ∼200–400 bp in length, and captured with streptavidin beads (Invitrogen), and washed with 1× saline sodium citrate (SSC) buffer to remove nonspecific binding, and nonbiotinylated strand containing the ligated 3′ adaptor was eluted with 0.15 m NaOH. .. After synthesis of the complementary strand with EconoTaq (Lucigen) and preparation of DNA ends with NEBNext End Repair and NEBNext dA-Tailing modules, a second adaptor ligation was performed followed by eight to 10 cycles of PCR amplification. .. Libraries were submitted for either Ion Torrent (WSU LBB1 Core Facility) or Illumina (Azenta) sequencing.

    Amplification:

    Article Title: Genome-wide maps of CPD deamination in yeast reveal the impact of DNA sequence context and nucleosome architecture on cytosine deamination rates
    Article Snippet: Ligated DNA was then sonicated (Diagenode Bioruptor 300) to achieve DNA fragments ∼200–400 bp in length, and captured with streptavidin beads (Invitrogen), and washed with 1× saline sodium citrate (SSC) buffer to remove nonspecific binding, and nonbiotinylated strand containing the ligated 3′ adaptor was eluted with 0.15 m NaOH. .. After synthesis of the complementary strand with EconoTaq (Lucigen) and preparation of DNA ends with NEBNext End Repair and NEBNext dA-Tailing modules, a second adaptor ligation was performed followed by eight to 10 cycles of PCR amplification. .. Libraries were submitted for either Ion Torrent (WSU LBB1 Core Facility) or Illumina (Azenta) sequencing.

    other:

    Article Title: Genome-wide regulon of NtrC reveals genetic regulation under nitrogen limitation in Methylomonas sp. DH-1
    Article Snippet: NEBNext® dA-Tailing Module , New England Biolabs , Cat#E6053S.



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