Review




Structured Review

Merck KGaA fluoroshieldtm with dapi
Immunofluorescence analysis performed on HEK293 cells ( a ) and human fibroblasts ( b ). The N-terminal antibody detected isoforms 1, 3.1 and 3.2 in the cytoplasm and nucleus. Interestingly, an association with centrosomes (white arrow) and kinetochores was detected. Staining with a C-terminal antibody, detecting the full-length isoform 1 and additional hypothetic short C-terminal isoforms, showed a mainly nuclear pattern without a co-localization with the mitotic spindle, centrosomes or kinetochores. α-Tubulin was used for cytoskeletal staining and nuclei were stained using <t>DAPI.</t> Images were obtained using a confocal laser microscope with × 600 magnification, and an additional software magnification as indicated in the respectively images
Fluoroshieldtm With Dapi, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluoroshield+with+dapi/fluoroshield+with+dapi/pmc07519918-74-8-11
Average 90 stars, based on 1 article reviews
fluoroshieldtm with dapi - by Bioz Stars, 2026-11
90/100 stars

Images

1) Product Images from "De novo mutations in FBRSL1 cause a novel recognizable malformation and intellectual disability syndrome"

Article Title: De novo mutations in FBRSL1 cause a novel recognizable malformation and intellectual disability syndrome

Journal: Human Genetics

doi: 10.1007/s00439-020-02175-x

Immunofluorescence analysis performed on HEK293 cells ( a ) and human fibroblasts ( b ). The N-terminal antibody detected isoforms 1, 3.1 and 3.2 in the cytoplasm and nucleus. Interestingly, an association with centrosomes (white arrow) and kinetochores was detected. Staining with a C-terminal antibody, detecting the full-length isoform 1 and additional hypothetic short C-terminal isoforms, showed a mainly nuclear pattern without a co-localization with the mitotic spindle, centrosomes or kinetochores. α-Tubulin was used for cytoskeletal staining and nuclei were stained using DAPI. Images were obtained using a confocal laser microscope with × 600 magnification, and an additional software magnification as indicated in the respectively images
Figure Legend Snippet: Immunofluorescence analysis performed on HEK293 cells ( a ) and human fibroblasts ( b ). The N-terminal antibody detected isoforms 1, 3.1 and 3.2 in the cytoplasm and nucleus. Interestingly, an association with centrosomes (white arrow) and kinetochores was detected. Staining with a C-terminal antibody, detecting the full-length isoform 1 and additional hypothetic short C-terminal isoforms, showed a mainly nuclear pattern without a co-localization with the mitotic spindle, centrosomes or kinetochores. α-Tubulin was used for cytoskeletal staining and nuclei were stained using DAPI. Images were obtained using a confocal laser microscope with × 600 magnification, and an additional software magnification as indicated in the respectively images

Techniques Used: Immunofluorescence, Staining, Microscopy, Software

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Staining:

Article Title: Selective Blockade of Two Aquaporin Channels, AQP3 and AQP9, Impairs Human Leukocyte Migration.
Article Snippet: .. Slides were mounted and nuclei stained with Fluoroshield-containing DAPI (Merck Group). .. Slides were observed with a Leica DMRXA fluorescent microscope and fluorescence images captured with a Leica DFC450 C camera and processed by the Leica Application Suite Advanced Fluorescence software (LAS AF 3.1.0 build 8587).

Article Title: Perinatal exposure to methadone or buprenorphine impairs hippocampal-dependent cognition and brain development in juvenile rats.
Article Snippet: The opioid crisis continues to escalate, disproportionately affecting women of reproductive age.. Traditionally the first line of treatment for pregnant women with opioid use disorder is the mu-opioid receptor agonist methadone.. However, in recent years, the use of buprenorphine as a replacement therapy has increased as it has fewer sideeffects and longer duration of action.

Article Title: Integration of multiple flexible electrodes for real-time detection of barrier formation with spatial resolution in a gut-on-chip system
Article Snippet: .. The cells were labeled with occludin antibody diluted in 4% BSA for 3 h at room temperature, and nuclei were stained overnight with Fluoroshield TM with DAPI (F6057, Merck Milipore, Hoeilaart, Belgium). .. Both occludin and cell nuclei were visualized using an inverted fluorescence microscope (IX83, Olympus, Antwerpen, Belgium) and CellSens Dimension software for image analysis to identify cell distribution and barrier confluency along the HuMiX channel.

Incubation:

Article Title: Perinatal exposure to methadone or buprenorphine impairs hippocampal-dependent cognition and brain development in juvenile rats.
Article Snippet: The opioid crisis continues to escalate, disproportionately affecting women of reproductive age.. Traditionally the first line of treatment for pregnant women with opioid use disorder is the mu-opioid receptor agonist methadone.. However, in recent years, the use of buprenorphine as a replacement therapy has increased as it has fewer sideeffects and longer duration of action.

Labeling:

Article Title: Integration of multiple flexible electrodes for real-time detection of barrier formation with spatial resolution in a gut-on-chip system
Article Snippet: .. The cells were labeled with occludin antibody diluted in 4% BSA for 3 h at room temperature, and nuclei were stained overnight with Fluoroshield TM with DAPI (F6057, Merck Milipore, Hoeilaart, Belgium). .. Both occludin and cell nuclei were visualized using an inverted fluorescence microscope (IX83, Olympus, Antwerpen, Belgium) and CellSens Dimension software for image analysis to identify cell distribution and barrier confluency along the HuMiX channel.

other:

Article Title: Tg1.4HBV-s-rec mice, a crossbred hepatitis B virus-transgenic model, develop mild hepatitis.
Article Snippet: Sections were washed with PBS and mounted using FluorshieldTM with DAPI (Merck Millipore, Burlington, USA).

Article Title: Adenosine A 2B receptors differently modulate oligodendrogliogenesis and myelination depending on their cellular localization.
Article Snippet: Cells were washed twice with PBS and then incubated in PBS solution containing 0.25% Triton X-100 (PBST; Merck, Germany, #X100).



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Progressive sialadenitis in the IL-14αTG mouse model of Sjögren's disease. (A) Submandibular and sublingual glands from 6- and a 12-month-old female IL-14αTG and C57BL/6 mice were subjected to hematoxylin and eosin staining to assess glandular inflammation; scale bar = 1 mm. (B) Immune cell focus surrounding blood vessels and excretory ducts in a 12-month-old IL-14αTG mouse SMG; scale bar = 100 µm. (C) Twelve-month-old and (D) 18-month-old female IL-14αTG mouse SMG and SLG cryosections were subjected to immunofluorescence staining using antibodies against aquaporin 5 (AQP5) acinar cell marker, CD45 pan-immune cell marker, B220 B cell marker, CD3 T cell marker, CD169 macrophage marker, CD11c dendritic cell marker or GL7 germinal center marker with <t>DAPI</t> nuclear counterstain; scale bars = 1 mm and 100 µm (inset).
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Image Search Results


Progressive sialadenitis in the IL-14αTG mouse model of Sjögren's disease. (A) Submandibular and sublingual glands from 6- and a 12-month-old female IL-14αTG and C57BL/6 mice were subjected to hematoxylin and eosin staining to assess glandular inflammation; scale bar = 1 mm. (B) Immune cell focus surrounding blood vessels and excretory ducts in a 12-month-old IL-14αTG mouse SMG; scale bar = 100 µm. (C) Twelve-month-old and (D) 18-month-old female IL-14αTG mouse SMG and SLG cryosections were subjected to immunofluorescence staining using antibodies against aquaporin 5 (AQP5) acinar cell marker, CD45 pan-immune cell marker, B220 B cell marker, CD3 T cell marker, CD169 macrophage marker, CD11c dendritic cell marker or GL7 germinal center marker with DAPI nuclear counterstain; scale bars = 1 mm and 100 µm (inset).

Journal: Frontiers in Dental Medicine

Article Title: Salivary gland transcriptomic analysis and immunophenotyping in the IL-14α transgenic mouse model of Sjögren's disease

doi: 10.3389/fdmed.2025.1612522

Figure Lengend Snippet: Progressive sialadenitis in the IL-14αTG mouse model of Sjögren's disease. (A) Submandibular and sublingual glands from 6- and a 12-month-old female IL-14αTG and C57BL/6 mice were subjected to hematoxylin and eosin staining to assess glandular inflammation; scale bar = 1 mm. (B) Immune cell focus surrounding blood vessels and excretory ducts in a 12-month-old IL-14αTG mouse SMG; scale bar = 100 µm. (C) Twelve-month-old and (D) 18-month-old female IL-14αTG mouse SMG and SLG cryosections were subjected to immunofluorescence staining using antibodies against aquaporin 5 (AQP5) acinar cell marker, CD45 pan-immune cell marker, B220 B cell marker, CD3 T cell marker, CD169 macrophage marker, CD11c dendritic cell marker or GL7 germinal center marker with DAPI nuclear counterstain; scale bars = 1 mm and 100 µm (inset).

Article Snippet: Slides were then washed in PBS and coverslips mounted using Fluoroshield with DAPI (MilliporeSigma).

Techniques: Staining, Immunofluorescence, Marker