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fluoroshield mounting medium with dapi  (Beyotime)


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    Structured Review

    Beyotime fluoroshield mounting medium with dapi
    Fluoroshield Mounting Medium With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 31805 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluoroshield+with+dapi/DAPI/pmc13124985-211-29-34
    Average 99 stars, based on 31805 article reviews
    fluoroshield mounting medium with dapi - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Immunomodulatory effects of biodegradable Mg–Cu–Zn alloy in esophageal cancer
    Article Snippet: .. After treatment, all cells were washed twice with PBS, and fixed with 4% paraformaldehyde for 15 min. After permeabilization with 0.5% Triton X-100 for 10 min, cells were stained with 4′,6-diamidino-2-phenylindole (DAPI, Beyotime, China) for 10 min at room temperature in the dark. .. Images from ten random fields were captured using a fluorescence microscope (CKX53, Olympus, Japan) and analyzed using ImageJ software.

    Article Title: A nature-derived, strong adhesive hydrogel microsphere for wet tissue repair: Multifunctional and clinical potential
    Article Snippet: In short, the cells were washed twice with PBS, fixed in 4 % paraformaldehyde (PFA, Solarbio, China) for 15 min, and then washed with PBS again. .. The cells were permeabilized with 0.2 %Triton X-100 solution for 15 min, followed by incubation with FITC-phalloidin dye (Yeasen, China) in the dark for 30 min, and then stained with 4’, 6-diamidino-2-phenylindole (DAPI, Beyotime, China) for 8 min. ..

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
    Article Snippet: Proliferation was assessed using an EdU fluorescence kit (Beyotime, C0078S) according to the manufacturer's instructions: cells were incubated with 10 μM EdU for 2 h, fixed with 4% paraformaldehyde (Beyotime, P0099), and sequentially stained with Apollo® 567 (Ribobio, C10310 ) and Hoechst 33342 (Beyotime, C1022); fluorescence images were acquired on a microscope (Olympus BX53, Japan), and EdU-positive percentages were calculated from five randomly selected fields. .. Apoptosis was evaluated by TUNEL staining (Beyotime, C1088) after identical pretreatment, with fixation and permeabilization followed by incubation with the TUNEL reaction mixture at 37 °C for 1 h in the dark and DAPI (Beyotime, P0131) counterstaining; images were collected on the same microscope and quantified in ImageJ. .. For quantitative apoptosis analysis by flow cytometry, chondrocytes were seeded in 6-well plates (1 × 10 6 cells per well), subjected to the same LPS induction and hydrogel treatment, harvested, washed twice with cold PBS, resuspended in 1 × binding buffer, and stained with Annexin V-APC/7-AAD (Beyotime, C1063L) for 15 min at room temperature in the dark; apoptotic fractions were determined on a BD LSRFortessa X-20 (USA).

    Article Title: Microenvironment-educated MSC-EVs loaded injectable smart hydrogel for targeting senescent nucleus pulposus cells and inhibiting ferroptosis against intervertebral disc degeneration
    Article Snippet: The pelleted EVs were pre-labeled with 10 μM PHK26 dye (Beyotime, C2017S) and then co-cultured with NPCs for 12 h, respectively. .. After fixation with 4% paraformaldehyde for 15 min, the Actin-tracker was used for cytoskeleton staining (Beyotime, C2201S), and the nucleus was stained with DAPI (Beyotime, C1002). .. The internalization of EVs was observed using confocal microscope (Olympus, FV3000).

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury
    Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), anti-Fluorescence Quencher (Contains DAPI) (P0131, Beyotime Biotechnology, China), Polymer-HRP Anti-Rabbit Secondary Antibody Kit (AFIHC003, AiFang biological, China), DAB Chromogenic Reagent Kit (ZLI-9019, ZSGB-BIO, China), RaPure Total RNA Kit (MD021, Magen, China), RNA Reversing Reagent Kit (RR047A, TaKaRa, Japan), ChamQTM Universal SYBR® qPCR Master Mix (Q711, Vazyme, China), Polymerase Chain Reaction Anti-Mouse IgG-HRP Kit (SV0001, Boster Biological Technology, China), Mouse IL-6 MPCLIA Kit (GLM0004, Boster Biological Technology, China) Isolation and purification of sEV by differential ultracentrifugation. ..

    Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens
    Article Snippet: Following blocking with 1% BSA, the sections were incubated with the primary antibody MYH1A (1:100, F59, DSHB, USA) and the AF647-labeled goat anti-mouse secondary antibody (1:500, A0473, Beyotime), with the primary antibody MYH7B (1:100, S58, DSHB) and the FITC-labeled goat anti-mouse secondary antibody (1:500, A0568, Beyotime). .. Cell nuclei were stained with DAPI (C1002, Beyotime). .. Finally, the sections were observed under a fluorescence microscope (Leica, Wetzlar, Germany) and the fluorescence area was calculated using ImageJ software.

    Incubation:

    Article Title: A nature-derived, strong adhesive hydrogel microsphere for wet tissue repair: Multifunctional and clinical potential
    Article Snippet: In short, the cells were washed twice with PBS, fixed in 4 % paraformaldehyde (PFA, Solarbio, China) for 15 min, and then washed with PBS again. .. The cells were permeabilized with 0.2 %Triton X-100 solution for 15 min, followed by incubation with FITC-phalloidin dye (Yeasen, China) in the dark for 30 min, and then stained with 4’, 6-diamidino-2-phenylindole (DAPI, Beyotime, China) for 8 min. ..

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
    Article Snippet: Proliferation was assessed using an EdU fluorescence kit (Beyotime, C0078S) according to the manufacturer's instructions: cells were incubated with 10 μM EdU for 2 h, fixed with 4% paraformaldehyde (Beyotime, P0099), and sequentially stained with Apollo® 567 (Ribobio, C10310 ) and Hoechst 33342 (Beyotime, C1022); fluorescence images were acquired on a microscope (Olympus BX53, Japan), and EdU-positive percentages were calculated from five randomly selected fields. .. Apoptosis was evaluated by TUNEL staining (Beyotime, C1088) after identical pretreatment, with fixation and permeabilization followed by incubation with the TUNEL reaction mixture at 37 °C for 1 h in the dark and DAPI (Beyotime, P0131) counterstaining; images were collected on the same microscope and quantified in ImageJ. .. For quantitative apoptosis analysis by flow cytometry, chondrocytes were seeded in 6-well plates (1 × 10 6 cells per well), subjected to the same LPS induction and hydrogel treatment, harvested, washed twice with cold PBS, resuspended in 1 × binding buffer, and stained with Annexin V-APC/7-AAD (Beyotime, C1063L) for 15 min at room temperature in the dark; apoptotic fractions were determined on a BD LSRFortessa X-20 (USA).

    Article Title: Mechanically sensitized hydrogel microspheres trigger membrane receptor switch for cartilage repair
    Article Snippet: .. The OBNC microspheres were added to the working solution according to the manufacturer's instructions, incubated for 30 min in the dark, and an anti-fluorescence quencher containing DAPI dye was added (No. P0131, Beyotime Biotechnology Co., LTD., China). .. F-actin was observed and images were acquired using a confocal laser microscope (STELLARIS5, Leica, GRE).

    TUNEL Assay:

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
    Article Snippet: Proliferation was assessed using an EdU fluorescence kit (Beyotime, C0078S) according to the manufacturer's instructions: cells were incubated with 10 μM EdU for 2 h, fixed with 4% paraformaldehyde (Beyotime, P0099), and sequentially stained with Apollo® 567 (Ribobio, C10310 ) and Hoechst 33342 (Beyotime, C1022); fluorescence images were acquired on a microscope (Olympus BX53, Japan), and EdU-positive percentages were calculated from five randomly selected fields. .. Apoptosis was evaluated by TUNEL staining (Beyotime, C1088) after identical pretreatment, with fixation and permeabilization followed by incubation with the TUNEL reaction mixture at 37 °C for 1 h in the dark and DAPI (Beyotime, P0131) counterstaining; images were collected on the same microscope and quantified in ImageJ. .. For quantitative apoptosis analysis by flow cytometry, chondrocytes were seeded in 6-well plates (1 × 10 6 cells per well), subjected to the same LPS induction and hydrogel treatment, harvested, washed twice with cold PBS, resuspended in 1 × binding buffer, and stained with Annexin V-APC/7-AAD (Beyotime, C1063L) for 15 min at room temperature in the dark; apoptotic fractions were determined on a BD LSRFortessa X-20 (USA).

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury
    Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), anti-Fluorescence Quencher (Contains DAPI) (P0131, Beyotime Biotechnology, China), Polymer-HRP Anti-Rabbit Secondary Antibody Kit (AFIHC003, AiFang biological, China), DAB Chromogenic Reagent Kit (ZLI-9019, ZSGB-BIO, China), RaPure Total RNA Kit (MD021, Magen, China), RNA Reversing Reagent Kit (RR047A, TaKaRa, Japan), ChamQTM Universal SYBR® qPCR Master Mix (Q711, Vazyme, China), Polymerase Chain Reaction Anti-Mouse IgG-HRP Kit (SV0001, Boster Biological Technology, China), Mouse IL-6 MPCLIA Kit (GLM0004, Boster Biological Technology, China) Isolation and purification of sEV by differential ultracentrifugation. ..

    Microscopy:

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
    Article Snippet: Proliferation was assessed using an EdU fluorescence kit (Beyotime, C0078S) according to the manufacturer's instructions: cells were incubated with 10 μM EdU for 2 h, fixed with 4% paraformaldehyde (Beyotime, P0099), and sequentially stained with Apollo® 567 (Ribobio, C10310 ) and Hoechst 33342 (Beyotime, C1022); fluorescence images were acquired on a microscope (Olympus BX53, Japan), and EdU-positive percentages were calculated from five randomly selected fields. .. Apoptosis was evaluated by TUNEL staining (Beyotime, C1088) after identical pretreatment, with fixation and permeabilization followed by incubation with the TUNEL reaction mixture at 37 °C for 1 h in the dark and DAPI (Beyotime, P0131) counterstaining; images were collected on the same microscope and quantified in ImageJ. .. For quantitative apoptosis analysis by flow cytometry, chondrocytes were seeded in 6-well plates (1 × 10 6 cells per well), subjected to the same LPS induction and hydrogel treatment, harvested, washed twice with cold PBS, resuspended in 1 × binding buffer, and stained with Annexin V-APC/7-AAD (Beyotime, C1063L) for 15 min at room temperature in the dark; apoptotic fractions were determined on a BD LSRFortessa X-20 (USA).

    Laser-Scanning Microscopy:

    Article Title: Spatiotemporally engineered microneedle for microenvironment remodeling propels mucosal regeneration after tracheal mucosal injury
    Article Snippet: Cells were then incubated overnight at 4 °C with rabbit polyclonal antibodies against CD86 or CD206 (1:200, Proteintech), followed by 1 h incubation with Alexa Fluor 488-conjugated or CoraLite Plus 594-conjugated goat anti-rabbit IgG secondary antibodies (1:200, Proteintech). .. Nuclei were counterstained with DAPI (1:100, Beyotime) for 5 min, and images were acquired using a confocal laser scanning microscope. .. Flow cytometry (Beckman Coulter, USA) was further employed to analyze the phenotypic changes of RAW264.7 cells.

    Apoptosis Assay:

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury
    Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), anti-Fluorescence Quencher (Contains DAPI) (P0131, Beyotime Biotechnology, China), Polymer-HRP Anti-Rabbit Secondary Antibody Kit (AFIHC003, AiFang biological, China), DAB Chromogenic Reagent Kit (ZLI-9019, ZSGB-BIO, China), RaPure Total RNA Kit (MD021, Magen, China), RNA Reversing Reagent Kit (RR047A, TaKaRa, Japan), ChamQTM Universal SYBR® qPCR Master Mix (Q711, Vazyme, China), Polymerase Chain Reaction Anti-Mouse IgG-HRP Kit (SV0001, Boster Biological Technology, China), Mouse IL-6 MPCLIA Kit (GLM0004, Boster Biological Technology, China) Isolation and purification of sEV by differential ultracentrifugation. ..

    Polymer:

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury
    Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), anti-Fluorescence Quencher (Contains DAPI) (P0131, Beyotime Biotechnology, China), Polymer-HRP Anti-Rabbit Secondary Antibody Kit (AFIHC003, AiFang biological, China), DAB Chromogenic Reagent Kit (ZLI-9019, ZSGB-BIO, China), RaPure Total RNA Kit (MD021, Magen, China), RNA Reversing Reagent Kit (RR047A, TaKaRa, Japan), ChamQTM Universal SYBR® qPCR Master Mix (Q711, Vazyme, China), Polymerase Chain Reaction Anti-Mouse IgG-HRP Kit (SV0001, Boster Biological Technology, China), Mouse IL-6 MPCLIA Kit (GLM0004, Boster Biological Technology, China) Isolation and purification of sEV by differential ultracentrifugation. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury
    Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), anti-Fluorescence Quencher (Contains DAPI) (P0131, Beyotime Biotechnology, China), Polymer-HRP Anti-Rabbit Secondary Antibody Kit (AFIHC003, AiFang biological, China), DAB Chromogenic Reagent Kit (ZLI-9019, ZSGB-BIO, China), RaPure Total RNA Kit (MD021, Magen, China), RNA Reversing Reagent Kit (RR047A, TaKaRa, Japan), ChamQTM Universal SYBR® qPCR Master Mix (Q711, Vazyme, China), Polymerase Chain Reaction Anti-Mouse IgG-HRP Kit (SV0001, Boster Biological Technology, China), Mouse IL-6 MPCLIA Kit (GLM0004, Boster Biological Technology, China) Isolation and purification of sEV by differential ultracentrifugation. ..

    Polymerase Chain Reaction:

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury
    Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), anti-Fluorescence Quencher (Contains DAPI) (P0131, Beyotime Biotechnology, China), Polymer-HRP Anti-Rabbit Secondary Antibody Kit (AFIHC003, AiFang biological, China), DAB Chromogenic Reagent Kit (ZLI-9019, ZSGB-BIO, China), RaPure Total RNA Kit (MD021, Magen, China), RNA Reversing Reagent Kit (RR047A, TaKaRa, Japan), ChamQTM Universal SYBR® qPCR Master Mix (Q711, Vazyme, China), Polymerase Chain Reaction Anti-Mouse IgG-HRP Kit (SV0001, Boster Biological Technology, China), Mouse IL-6 MPCLIA Kit (GLM0004, Boster Biological Technology, China) Isolation and purification of sEV by differential ultracentrifugation. ..

    Isolation:

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury
    Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), anti-Fluorescence Quencher (Contains DAPI) (P0131, Beyotime Biotechnology, China), Polymer-HRP Anti-Rabbit Secondary Antibody Kit (AFIHC003, AiFang biological, China), DAB Chromogenic Reagent Kit (ZLI-9019, ZSGB-BIO, China), RaPure Total RNA Kit (MD021, Magen, China), RNA Reversing Reagent Kit (RR047A, TaKaRa, Japan), ChamQTM Universal SYBR® qPCR Master Mix (Q711, Vazyme, China), Polymerase Chain Reaction Anti-Mouse IgG-HRP Kit (SV0001, Boster Biological Technology, China), Mouse IL-6 MPCLIA Kit (GLM0004, Boster Biological Technology, China) Isolation and purification of sEV by differential ultracentrifugation. ..

    Purification:

    Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury
    Article Snippet: .. The following were used: Nissl staining (G1036, Servicebio, China), One Step TUNEL Apoptosis Assay Kit (MA0224, Meilunbio, China), anti-Fluorescence Quencher (Contains DAPI) (P0131, Beyotime Biotechnology, China), Polymer-HRP Anti-Rabbit Secondary Antibody Kit (AFIHC003, AiFang biological, China), DAB Chromogenic Reagent Kit (ZLI-9019, ZSGB-BIO, China), RaPure Total RNA Kit (MD021, Magen, China), RNA Reversing Reagent Kit (RR047A, TaKaRa, Japan), ChamQTM Universal SYBR® qPCR Master Mix (Q711, Vazyme, China), Polymerase Chain Reaction Anti-Mouse IgG-HRP Kit (SV0001, Boster Biological Technology, China), Mouse IL-6 MPCLIA Kit (GLM0004, Boster Biological Technology, China) Isolation and purification of sEV by differential ultracentrifugation. ..



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    Progressive sialadenitis in the IL-14αTG mouse model of Sjögren's disease. (A) Submandibular and sublingual glands from 6- and a 12-month-old female IL-14αTG and C57BL/6 mice were subjected to hematoxylin and eosin staining to assess glandular inflammation; scale bar = 1 mm. (B) Immune cell focus surrounding blood vessels and excretory ducts in a 12-month-old IL-14αTG mouse SMG; scale bar = 100 µm. (C) Twelve-month-old and (D) 18-month-old female IL-14αTG mouse SMG and SLG cryosections were subjected to immunofluorescence staining using antibodies against aquaporin 5 (AQP5) acinar cell marker, CD45 pan-immune cell marker, B220 B cell marker, CD3 T cell marker, CD169 macrophage marker, CD11c dendritic cell marker or GL7 germinal center marker with <t>DAPI</t> nuclear counterstain; scale bars = 1 mm and 100 µm (inset).
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    Image Search Results


    Progressive sialadenitis in the IL-14αTG mouse model of Sjögren's disease. (A) Submandibular and sublingual glands from 6- and a 12-month-old female IL-14αTG and C57BL/6 mice were subjected to hematoxylin and eosin staining to assess glandular inflammation; scale bar = 1 mm. (B) Immune cell focus surrounding blood vessels and excretory ducts in a 12-month-old IL-14αTG mouse SMG; scale bar = 100 µm. (C) Twelve-month-old and (D) 18-month-old female IL-14αTG mouse SMG and SLG cryosections were subjected to immunofluorescence staining using antibodies against aquaporin 5 (AQP5) acinar cell marker, CD45 pan-immune cell marker, B220 B cell marker, CD3 T cell marker, CD169 macrophage marker, CD11c dendritic cell marker or GL7 germinal center marker with DAPI nuclear counterstain; scale bars = 1 mm and 100 µm (inset).

    Journal: Frontiers in Dental Medicine

    Article Title: Salivary gland transcriptomic analysis and immunophenotyping in the IL-14α transgenic mouse model of Sjögren's disease

    doi: 10.3389/fdmed.2025.1612522

    Figure Lengend Snippet: Progressive sialadenitis in the IL-14αTG mouse model of Sjögren's disease. (A) Submandibular and sublingual glands from 6- and a 12-month-old female IL-14αTG and C57BL/6 mice were subjected to hematoxylin and eosin staining to assess glandular inflammation; scale bar = 1 mm. (B) Immune cell focus surrounding blood vessels and excretory ducts in a 12-month-old IL-14αTG mouse SMG; scale bar = 100 µm. (C) Twelve-month-old and (D) 18-month-old female IL-14αTG mouse SMG and SLG cryosections were subjected to immunofluorescence staining using antibodies against aquaporin 5 (AQP5) acinar cell marker, CD45 pan-immune cell marker, B220 B cell marker, CD3 T cell marker, CD169 macrophage marker, CD11c dendritic cell marker or GL7 germinal center marker with DAPI nuclear counterstain; scale bars = 1 mm and 100 µm (inset).

    Article Snippet: Slides were then washed in PBS and coverslips mounted using Fluoroshield with DAPI (MilliporeSigma).

    Techniques: Staining, Immunofluorescence, Marker