fluoroshield with 4',6-diamidino-2-phenylindole dihydrochloride (dapi) (Merck KGaA)

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Merck KGaA fluoroshield with 4',6-diamidino-2-phenylindole dihydrochloride (dapi)
Fluoroshield With 4',6 Diamidino 2 Phenylindole Dihydrochloride (Dapi), supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluoroshield+dapi/fluoroshield+with+4++6+diamidino+2+phenylindole+dihydrochloride++dapi+/pmc07036901-144-13-18
Average 90 stars, based on 1 article reviews

fluoroshield with 4',6-diamidino-2-phenylindole dihydrochloride (dapi) - by Bioz Stars, 2026-10

90/100 stars

Images

Incubation:

Article Title: Virulence Characterization and Molecular Subtyping of Typical and Atypical Escherichia coli O157:H7 and O157:H(-) Isolated from Fecal Samples and Beef Carcasses in Mexico
Article Snippet: .. The objective of the study was to characterize virulence genes and subtype Escherichia coli O157:H7 and O157:H(2) isolates obtained from a vertically integrated feedlot slaughter plant in Mexico.. A total of 1,695 samples were collected from feedlots, holding pens, colon contents, hides, and carcasses.. E. coli O157:H7 detection and confirmation was carried out using conventional microbiology techniques, immunomagnetic separation, latex agglutination, and the BAX system. ..

Article Title: Apoptotic Effects on HL60 Human Leukaemia Cells Induced by Lavandin Essential Oil Treatment
Article Snippet: .. After three washings in PBS, nucleic acid staining was obtained by incubation with Fluoroshield with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) (Merck KGaA, Darmstadt, Germany), and then samples were placed. .. The images were captured by a Zeiss LSM 710 Confocal Microscope (Carl Zeiss, Oberkochen, Germany.

other:

Article Title: Apoptotic Effects on HL60 Human Leukaemia Cells Induced by Lavandin Essential Oil Treatment
Article Snippet: After rinsing three times in PBS, the coverslips were dried and incubated with an Alexa Fluor 488 Goat Anti-Mouse IgG Antibody (Molecular Probes, Eugene, OR, USA) diluted to 1:100 in a blocking buffer.

Article Title: miR-199a decreases Neuritin expression involved in the development of Alzheimer's disease in APP/PS1 mice
Article Snippet: HeLa and 293T cells from the Cell Bank of the Chinese Academy of Sciences were cultured in Dulbecco's modified Eagle's medium (DMEM) with 4,500 mg/l glucose (Sigma-Aldrich; Merck KGaA) and 10% fetal bovine serum (Biological Industries).

Staining:

Article Title: Recombinant Type XVII Collagen Inhibits EGFR/MAPK/AP-1 and Activates TGF-β/Smad Signaling to Enhance Collagen Secretion and Reduce Photoaging
Article Snippet: .. Next, the cells were fixed on glass coverslips and stained at 4 ◦C overnight with anti-COL17 antibody (ab231939; Abcam), antiCOL4 antibody (ab6586; Abcam), recombinant anti-LAMA5 antibody (ab318962; Abcam), and anti-integrin β1 antibody (ab24693; Abcam) conjugated with Alexa Fluor 488 (ab150077; Abcam) and ProLong Gold Antifade Reagent with 4′,6-diamidino-2-phenylindole (cat. no. 28718-90-3; Merck Millipore, Burlington, MA, USA) to visualize the nuclei. .. The cell fixation and staining procedures were performed using an Immunofluorescence Application Solutions Kit (cat. no. #12727; CST) according to the manufacturer’s instructions.

Recombinant:

Isolation:

Article Title: Effect of Naturally Occurring Biogenic Materials on Human Dental Pulp Stem Cells (hDPSC): an In Vitro Study.
Article Snippet: Objectives This study aimed to evaluate and compare the cell viability and cell differentiation potential together with the antiinflammatory potential of coral (Now Foods Calcium Carbonate Powder, HerbsPro, India) and BiodentineTM (Septodont, StMaur-des-Fosses, Cedex, France) on human dental pulp stem cells (hDPSC).. Materials and Methods Human dental pulp stem cells (hDPSC) were segregated and cultured from the dental pulp of healthy teeth undergoing therapeutic extraction.. For assessing cell proliferation potential of coral and Biodentine, the agents were taken at a concentration (800, 400, 200, 100, 50 μg/mL) and for cell differentiation potential a concentration of 400 μg/mL respectively.

Saline:

Article Title: A bivalent spike-targeting nanobody with anti-sarbecovirus activity.
Article Snippet: Two consecutive rounds of panning were performed, for the first round 0.5 μg/mL SARS-CoV-2-RBD protein was coated onto a NUNC Maxisorp strip (Thermo Fisher Scientific) at 4 °C overnight. .. Coated wells were washed 3 × using Phosphate-Buffered Saline (PBS) supplemented with 0.1% Tween-20 (PBS-T) and blocked using PBS supplemented with 4% w/v skimmed milk powder (PBSM-4%; Merck Millipore) for 1h at room temperature (RT). ..

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