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ImmunoBioScience Corp fluoroshield with dapi
Fluoroshield With Dapi, supplied by ImmunoBioScience Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluoroshield+dapi/fluoroshield+with+dapi/pm33458442-71-6-9
Average 90 stars, based on 1 article reviews
fluoroshield with dapi - by Bioz Stars, 2026-09
90/100 stars

Images

Related Articles

Fluorescence:

Article Title: Methionine Restriction Attenuates Scar Formation in Fibroblasts Derived from Patients with Post-Burn Hypertrophic Scar
Article Snippet: After three washes with PBST, the cells were incubated with the corresponding secondary antibodies (Alexa Fluor® 488– conjugated; Thermo Fisher Scientific, Waltham, MA, USA) for 1 h at room temperature in the dark, followed by four additional washes with PBST. .. Finally, the cells were mounted using FluoroShield containing DAPI (ImmunoBioScience Corp., Davis, CA, USA) for 1 min. Cover glasses were subsequently applied and cell images were captured under a fluorescence microscope at ×10 and ×40 magnification (IX 81; Olympus). ..

Article Title: Mesenchymal Stem Cells and Extracellular Vesicles Derived from Canine Adipose Tissue Ameliorates Inflammation, Skin Barrier Function and Pruritus by Reducing JAK/STAT Signaling in Atopic Dermatitis.
Article Snippet: After staining, tissues were dehydrated and sealed in Permount (SP15-100; Thermo Fisher Scientific), and observed via optical microscopy (DM750, Leica, Wetzlar, Germany). .. For fluorescence images, the sections were incubated with primary antibody with anti-TRPA1 (GTX54765; GeneTex, Irvine, CA, USA) antibody at 4 ◦C for 16 h, followed by incubation with secondary antibodies against FITC-conjugated goat-anti-rabbit IgG (1:1000, sc-2012, Santa Cruz Biotechnology) at 21 ◦C for 1 h. Immunostained tissues were mounted with a medium containing FluoroshieldTM with DAPI (ImmunoBioScience, Mukilteo, WA, USA). .. Fluorescence images were acquired by using a confocal microscope (LSM700; Zeiss, Jena, Germany).

Article Title: Mesenchymal Stem Cells and Extracellular Vesicles Derived from Canine Adipose Tissue Ameliorates Inflammation, Skin Barrier Function and Pruritus by Reducing JAK/STAT Signaling in Atopic Dermatitis
Article Snippet: After staining, tissues were dehydrated and sealed in Permount (SP15-100; Thermo Fisher Scientific), and observed via optical microscopy (DM750, Leica, Wetzlar, Germany). .. For fluorescence images, the sections were incubated with primary antibody with anti-TRPA1 (GTX54765; GeneTex, Irvine, CA, USA) antibody at 4 °C for 16 h, followed by incubation with secondary antibodies against FITC-conjugated goat-anti-rabbit IgG (1:1000, sc-2012, Santa Cruz Biotechnology) at 21 °C for 1 h. Immunostained tissues were mounted with a medium containing FluoroshieldTM with DAPI (ImmunoBioScience, Mukilteo, WA, USA). .. Fluorescence images were acquired by using a confocal microscope (LSM700; Zeiss, Jena, Germany).

Microscopy:

Article Title: Methionine Restriction Attenuates Scar Formation in Fibroblasts Derived from Patients with Post-Burn Hypertrophic Scar
Article Snippet: After three washes with PBST, the cells were incubated with the corresponding secondary antibodies (Alexa Fluor® 488– conjugated; Thermo Fisher Scientific, Waltham, MA, USA) for 1 h at room temperature in the dark, followed by four additional washes with PBST. .. Finally, the cells were mounted using FluoroShield containing DAPI (ImmunoBioScience Corp., Davis, CA, USA) for 1 min. Cover glasses were subsequently applied and cell images were captured under a fluorescence microscope at ×10 and ×40 magnification (IX 81; Olympus). ..

Incubation:

Article Title: Mesenchymal Stem Cells and Extracellular Vesicles Derived from Canine Adipose Tissue Ameliorates Inflammation, Skin Barrier Function and Pruritus by Reducing JAK/STAT Signaling in Atopic Dermatitis.
Article Snippet: After staining, tissues were dehydrated and sealed in Permount (SP15-100; Thermo Fisher Scientific), and observed via optical microscopy (DM750, Leica, Wetzlar, Germany). .. For fluorescence images, the sections were incubated with primary antibody with anti-TRPA1 (GTX54765; GeneTex, Irvine, CA, USA) antibody at 4 ◦C for 16 h, followed by incubation with secondary antibodies against FITC-conjugated goat-anti-rabbit IgG (1:1000, sc-2012, Santa Cruz Biotechnology) at 21 ◦C for 1 h. Immunostained tissues were mounted with a medium containing FluoroshieldTM with DAPI (ImmunoBioScience, Mukilteo, WA, USA). .. Fluorescence images were acquired by using a confocal microscope (LSM700; Zeiss, Jena, Germany).

Article Title: Mesenchymal Stem Cells and Extracellular Vesicles Derived from Canine Adipose Tissue Ameliorates Inflammation, Skin Barrier Function and Pruritus by Reducing JAK/STAT Signaling in Atopic Dermatitis
Article Snippet: After staining, tissues were dehydrated and sealed in Permount (SP15-100; Thermo Fisher Scientific), and observed via optical microscopy (DM750, Leica, Wetzlar, Germany). .. For fluorescence images, the sections were incubated with primary antibody with anti-TRPA1 (GTX54765; GeneTex, Irvine, CA, USA) antibody at 4 °C for 16 h, followed by incubation with secondary antibodies against FITC-conjugated goat-anti-rabbit IgG (1:1000, sc-2012, Santa Cruz Biotechnology) at 21 °C for 1 h. Immunostained tissues were mounted with a medium containing FluoroshieldTM with DAPI (ImmunoBioScience, Mukilteo, WA, USA). .. Fluorescence images were acquired by using a confocal microscope (LSM700; Zeiss, Jena, Germany).

Article Title: Inhibitory effect of PPARγ on NLRP3 inflammasome activation
Article Snippet: .. After washing with cold 1X PBS, samples were incubated with the secondary antibodies for 1 h at RT, and then mounted in Fluoroshield with DAPI of mounting media (ImmunoBioScience, Mukilteo, Washington, USA). .. The images were visualized by confocal microscopy (C1-Si, Nikon, Tokyo, Japan) with a 60× oil objective lens.



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Progressive sialadenitis in the IL-14αTG mouse model of Sjögren's disease. (A) Submandibular and sublingual glands from 6- and a 12-month-old female IL-14αTG and C57BL/6 mice were subjected to hematoxylin and eosin staining to assess glandular inflammation; scale bar = 1 mm. (B) Immune cell focus surrounding blood vessels and excretory ducts in a 12-month-old IL-14αTG mouse SMG; scale bar = 100 µm. (C) Twelve-month-old and (D) 18-month-old female IL-14αTG mouse SMG and SLG cryosections were subjected to immunofluorescence staining using antibodies against aquaporin 5 (AQP5) acinar cell marker, CD45 pan-immune cell marker, B220 B cell marker, CD3 T cell marker, CD169 macrophage marker, CD11c dendritic cell marker or GL7 germinal center marker with <t>DAPI</t> nuclear counterstain; scale bars = 1 mm and 100 µm (inset).
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Image Search Results


Progressive sialadenitis in the IL-14αTG mouse model of Sjögren's disease. (A) Submandibular and sublingual glands from 6- and a 12-month-old female IL-14αTG and C57BL/6 mice were subjected to hematoxylin and eosin staining to assess glandular inflammation; scale bar = 1 mm. (B) Immune cell focus surrounding blood vessels and excretory ducts in a 12-month-old IL-14αTG mouse SMG; scale bar = 100 µm. (C) Twelve-month-old and (D) 18-month-old female IL-14αTG mouse SMG and SLG cryosections were subjected to immunofluorescence staining using antibodies against aquaporin 5 (AQP5) acinar cell marker, CD45 pan-immune cell marker, B220 B cell marker, CD3 T cell marker, CD169 macrophage marker, CD11c dendritic cell marker or GL7 germinal center marker with DAPI nuclear counterstain; scale bars = 1 mm and 100 µm (inset).

Journal: Frontiers in Dental Medicine

Article Title: Salivary gland transcriptomic analysis and immunophenotyping in the IL-14α transgenic mouse model of Sjögren's disease

doi: 10.3389/fdmed.2025.1612522

Figure Lengend Snippet: Progressive sialadenitis in the IL-14αTG mouse model of Sjögren's disease. (A) Submandibular and sublingual glands from 6- and a 12-month-old female IL-14αTG and C57BL/6 mice were subjected to hematoxylin and eosin staining to assess glandular inflammation; scale bar = 1 mm. (B) Immune cell focus surrounding blood vessels and excretory ducts in a 12-month-old IL-14αTG mouse SMG; scale bar = 100 µm. (C) Twelve-month-old and (D) 18-month-old female IL-14αTG mouse SMG and SLG cryosections were subjected to immunofluorescence staining using antibodies against aquaporin 5 (AQP5) acinar cell marker, CD45 pan-immune cell marker, B220 B cell marker, CD3 T cell marker, CD169 macrophage marker, CD11c dendritic cell marker or GL7 germinal center marker with DAPI nuclear counterstain; scale bars = 1 mm and 100 µm (inset).

Article Snippet: Slides were then washed in PBS and coverslips mounted using Fluoroshield with DAPI (MilliporeSigma).

Techniques: Staining, Immunofluorescence, Marker