dapi (Beyotime)
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Beyotime
dapi
Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 33153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescence+microscopy+with+dapi/DAPI/pmc12784635-100-4-5
Average 99 stars, based on 33153 article reviews
Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 33153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescence+microscopy+with+dapi/DAPI/pmc12784635-100-4-5
Average 99 stars, based on 33153 article reviews
dapi - by Bioz Stars,
2026-11
99/100 stars
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Staining:Article Title: SATB1 lowers the T cell receptor signaling threshold for thymocyte positive selection via super-enhancer reprogramming. Article Snippet: Followed by 3 × PBS wash, followed by incubation with a secondary antibody in conjunctionwithAlexa 647fluorescence (FD0144) 1:1000 in 1 × PBS for onehour in the dark. .. Afterwashing three times, the nuclei were stained with 10 μg/ml Article Title: CD147 drives keloid formation by promoting fibroblast proliferation via the CENPF signaling pathway. Article Snippet: After being inoculated onto 24-well culture plates and subjected to the indicated treatments, the CoraLite488 TUNEL assay apoptosis detection kit (Proteintech, Wuhan, China) was used to detect apoptotic cells according to the manufacturer’s instructions. .. Nuclei were stained with 4′,6- Article Title: The AGEs/RAGE Axis Contributes to Myocardial Cell Death in Type 1 Diabetic Cardiomyopathy via the ROS/NF‐ κ B/NLRP3 Inflammasome Pathway Article Snippet: β ‐Actin (Cat#R1207‐1) was purchased from Huabio (Harbin, China). .. Trypsin solution (Cat#C0201), CM‐H2DCFDA staining (Cat#S0035S), Cy3‐conjugated secondary antibody (Cat# A0521), FITC‐conjugated secondary antibody (Cat# A0568), 4 ′ , Blocking Assay:Article Title: NSUN3‐Associated m5C Modification of tRF5‐GlyTCC Suppresses Colorectal Cancer Progression via RPL23‐Linked Nucleolar Stress and p53‐Associated Autophagy and Enhances 5‐FU Sensitivity Article Snippet: .. Cells were fixed with 4% paraformaldehyde for 15 min and permeabilized with 0.1% Triton X‐100 for 10 min. After blocking with 5% BSA for 1 h, cells were incubated with an anti‐NPM1 antibody (Abcam, UK) overnight at 4°C, followed by incubation with an Alexa Fluor‐conjugated secondary antibody for 1 h. Nuclei were counterstained with Incubation:Article Title: NSUN3‐Associated m5C Modification of tRF5‐GlyTCC Suppresses Colorectal Cancer Progression via RPL23‐Linked Nucleolar Stress and p53‐Associated Autophagy and Enhances 5‐FU Sensitivity Article Snippet: .. Cells were fixed with 4% paraformaldehyde for 15 min and permeabilized with 0.1% Triton X‐100 for 10 min. After blocking with 5% BSA for 1 h, cells were incubated with an anti‐NPM1 antibody (Abcam, UK) overnight at 4°C, followed by incubation with an Alexa Fluor‐conjugated secondary antibody for 1 h. Nuclei were counterstained with Article Title: Lineage-specific and gestational age–dependent DNA methylation programming orchestrates human placental development Article Snippet: Then, they were incubated with 5mC (61255, Active Motif), EGFR (ab30, Abcam), HLA-G (ab52454, Abcam), TFAP2C (sc12762, Santa Cruz Biotechnology), CDH2 (ab18203, Abcam), EpCAM (MA5-12436, Thermo Fisher Scientific), TP63 (ab124762, Abcam), SDC1 (67155-1-Ig, Proteintech), CGB (ab9582, Abcam), or α-SMA (MA1-06110, Thermo Fisher Scientific) overnight at 4°C. .. After multiple washes to remove residual primary antibody, cells or sections were then incubated with AF488/AF594-conjugated secondary antibodies (Thermo Fisher Scientific) for 1 hour at room temperature and mounted in prolonged anti-fade reagent with TUNEL Assay:Article Title: CD147 drives keloid formation by promoting fibroblast proliferation via the CENPF signaling pathway. 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Article Snippet: After being inoculated onto 24-well culture plates and subjected to the indicated treatments, the CoraLite488 TUNEL assay apoptosis detection kit (Proteintech, Wuhan, China) was used to detect apoptotic cells according to the manufacturer’s instructions. .. Nuclei were stained with 4′,6- Microscopy:Article Title: CD147 drives keloid formation by promoting fibroblast proliferation via the CENPF signaling pathway. Article Snippet: After being inoculated onto 24-well culture plates and subjected to the indicated treatments, the CoraLite488 TUNEL assay apoptosis detection kit (Proteintech, Wuhan, China) was used to detect apoptotic cells according to the manufacturer’s instructions. .. Nuclei were stained with 4′,6- BIA-KA:Article Title: The AGEs/RAGE Axis Contributes to Myocardial Cell Death in Type 1 Diabetic Cardiomyopathy via the ROS/NF‐ κ B/NLRP3 Inflammasome Pathway Article Snippet: β ‐Actin (Cat#R1207‐1) was purchased from Huabio (Harbin, China). .. Trypsin solution (Cat#C0201), CM‐H2DCFDA staining (Cat#S0035S), Cy3‐conjugated secondary antibody (Cat# A0521), FITC‐conjugated secondary antibody (Cat# A0568), 4 ′ , Protein Concentration:Article Title: The AGEs/RAGE Axis Contributes to Myocardial Cell Death in Type 1 Diabetic Cardiomyopathy via the ROS/NF‐ κ B/NLRP3 Inflammasome Pathway Article Snippet: β ‐Actin (Cat#R1207‐1) was purchased from Huabio (Harbin, China). .. Trypsin solution (Cat#C0201), CM‐H2DCFDA staining (Cat#S0035S), Cy3‐conjugated secondary antibody (Cat# A0521), FITC‐conjugated secondary antibody (Cat# A0568), 4 ′ , LDH Cytotoxicity Assay:Article Title: The AGEs/RAGE Axis Contributes to Myocardial Cell Death in Type 1 Diabetic Cardiomyopathy via the ROS/NF‐ κ B/NLRP3 Inflammasome Pathway Article Snippet: β ‐Actin (Cat#R1207‐1) was purchased from Huabio (Harbin, China). .. Trypsin solution (Cat#C0201), CM‐H2DCFDA staining (Cat#S0035S), Cy3‐conjugated secondary antibody (Cat# A0521), FITC‐conjugated secondary antibody (Cat# A0568), 4 ′ , |
![Combining fluorescence microscopy with multiplex IMC data of colorectal tissue advances quality of single cell segmentation. a Cartoon describing MATISSE, a novel pipeline adding microscopic imaging to multiplex IMC analysis and downstream segmentation. In short: tissue sections on slides were stained using isotope-conjugated primary antibodies, DNA intercalator, and <t>DAPI.</t> The tissue was first scanned using <t>a</t> <t>fluorescent</t> microscope and then processed with IMC. Data produced by both techniques is aligned using the nuclear staining. Nuclear and membranous pixel probability maps are produced based on the fluorescent images and IMC data respectively. These probability maps are used to generate a segmentation map, where all detected cells are included. b Representative images of DNA intercalator on a colorectal tissue section analyzed by Ir193 labeling and IMC (left) or DAPI labeling and fluorescent microscopy (IF, right). c IMC-only (IMC) and MATISSE cell segmentation (MATISSE) were performed, and shown are the different predicted outlines on a representative image of Ir193 labeling. Arrows indicate areas with cell fragmentation. d Display of a large region of interest (ROI) showing an overlay of the predicted cell outlines (pink) upon IMC or MATISSE segmentation on a representative IMC image of DNA-Ir193 labeling of colorectal tissue. Highlighted in yellow is the approximate position of the basement membrane surrounding the epithelial monolayer. Scale bar 25 μm. e Cell density was calculated as the number of cells within a radius of 10 μM from the center of each single cell [ , ]. This number is displayed with a color code for each cell in the representative image](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4487/pmc08114487/pmc08114487__12915_2021_1043_Fig1_HTML.jpg)