dapi (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
dapi
Dapi, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescence+microscopy+with+dapi/DAPI/pmc08450127-78-5-6
Average 98 stars, based on 2155 article reviews
Dapi, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescence+microscopy+with+dapi/DAPI/pmc08450127-78-5-6
Average 98 stars, based on 2155 article reviews
dapi - by Bioz Stars,
2026-10
98/100 stars
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Incubation:Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms. Article Snippet: Sections were then incubated with primary antibodies against UCP1 (Abcam, ab10983), PPARγ (Santa Cruz Biotechnology, sc-7273), and PDGFRα (R&D Systems, AF-307-NA) at 4 °C overnight. .. After washing, sections were incubated with appropriate fluorophore-conjugated secondary antibodies for 1 h at room temperature and counterstained with Article Title: Investigating the Effect and Mechanism of Protocatechuic Aldehyde on Vascular Dementia Based on Multi-Omics Approach. Article Snippet: .. The 300 μL blocking solution was added and blocked at 25 ◦C for 1 h. The slides were placed in primary antibodies, including monocarboxylate Transporter 1 [MCT1], MCT2 (PA5-72957, PA5-76603, Thermo Fisher Scientific, Pittsburgh, PA, USA), MCT4, GFAP, NeuN (22787-1-AP, 60190-1-Ig, 26975-1-AP, Proteintech, Chicago, IL, USA), Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms. Article Snippet: .. Cells were then incubated with primary antibodies against PPARγ diluted in blocking buffer at 4 °C overnight, followed by incubation with appropriate fluorophoreconjugated secondary antibodies at room temperature for 1 h. Blocking Assay:Article Title: Investigating the Effect and Mechanism of Protocatechuic Aldehyde on Vascular Dementia Based on Multi-Omics Approach. Article Snippet: .. The 300 μL blocking solution was added and blocked at 25 ◦C for 1 h. The slides were placed in primary antibodies, including monocarboxylate Transporter 1 [MCT1], MCT2 (PA5-72957, PA5-76603, Thermo Fisher Scientific, Pittsburgh, PA, USA), MCT4, GFAP, NeuN (22787-1-AP, 60190-1-Ig, 26975-1-AP, Proteintech, Chicago, IL, USA), Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms. Article Snippet: .. Cells were then incubated with primary antibodies against PPARγ diluted in blocking buffer at 4 °C overnight, followed by incubation with appropriate fluorophoreconjugated secondary antibodies at room temperature for 1 h. Staining:Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms. Article Snippet: .. Cells were washed once with PBS, fixed in 4% paraformaldehyde for 15 min and stained with 0.5 μM Nile red solution (Sigma, N3013) and 1ng/mL Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms. Article Snippet: .. Nile red staining Cells were washed once with PBS, fixed in 4% paraformaldehyde for 15 min and stained with 0.5 μM Nile red solution (Sigma, N3013) and 1ng/mL |
![Combining fluorescence microscopy with multiplex IMC data of colorectal tissue advances quality of single cell segmentation. a Cartoon describing MATISSE, a novel pipeline adding microscopic imaging to multiplex IMC analysis and downstream segmentation. In short: tissue sections on slides were stained using isotope-conjugated primary antibodies, DNA intercalator, and <t>DAPI.</t> The tissue was first scanned using <t>a</t> <t>fluorescent</t> microscope and then processed with IMC. Data produced by both techniques is aligned using the nuclear staining. Nuclear and membranous pixel probability maps are produced based on the fluorescent images and IMC data respectively. These probability maps are used to generate a segmentation map, where all detected cells are included. b Representative images of DNA intercalator on a colorectal tissue section analyzed by Ir193 labeling and IMC (left) or DAPI labeling and fluorescent microscopy (IF, right). c IMC-only (IMC) and MATISSE cell segmentation (MATISSE) were performed, and shown are the different predicted outlines on a representative image of Ir193 labeling. Arrows indicate areas with cell fragmentation. d Display of a large region of interest (ROI) showing an overlay of the predicted cell outlines (pink) upon IMC or MATISSE segmentation on a representative IMC image of DNA-Ir193 labeling of colorectal tissue. Highlighted in yellow is the approximate position of the basement membrane surrounding the epithelial monolayer. Scale bar 25 μm. e Cell density was calculated as the number of cells within a radius of 10 μM from the center of each single cell [ , ]. This number is displayed with a color code for each cell in the representative image](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4487/pmc08114487/pmc08114487__12915_2021_1043_Fig1_HTML.jpg)