upright fluorescence microscope (Nikon)
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Nikon
upright fluorescence microscope
Upright Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescence+microscope/C2%2B/pmc12966746-159-5-8
Average 99 stars, based on 1 article reviews
Upright Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fluorescence+microscope/C2%2B/pmc12966746-159-5-8
Average 99 stars, based on 1 article reviews
upright fluorescence microscope - by Bioz Stars,
2026-10
99/100 stars
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Microscopy:Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7 Article Snippet: .. The biofilms were observed with a Nikon Article Title: Sustained release PLGA microspheres loaded with a bone-affinity Bmp2 enhance fracture healing and mitigate heterotopic ossification Article Snippet: The samples were then incubated with a Cy3-conjugated secondary antibody (Servicebio, China) for 50 min. .. Images were captured using an Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ), cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h, washed twice with PBS, and incubated with JC-1 working solution (Beyotime, C2006) at 37 °C for 30 min in the dark. .. Following three PBS washes, red (aggregates) and green (monomers) fluorescence were acquired on a Article Title: Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptake Article Snippet: .. Confocal imaging was performed using a laser scanning confocal microscope (NIKON Eclipse Ti) equipped with the Article Title: Norepinephrine neurons in the locus coeruleus and the nucleus of the solitary tract drive different stress-related behavioral outputs in mice Article Snippet: .. They were then frozen and sectioned at 30 μm on a freezing microtome (HM550, Microm), mounted on microscope slides with Fluoroshield with DAPI mounting medium (F6057, Sigma-Aldrich), coverslipped, and imaged on a Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: Rat chondrocytes (P1) were seeded in 24-well plates (5 × 10 4 cells per well) and cultured to ∼70–80% confluence, stimulated with LPS (10 μg mL −1 , 12 h) to induce an inflammatory phenotype, and then treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were washed three times with PBS, fixed in 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X-100 (Aladdin, T434386) for 10 min, and blocked with 5% BSA (Solarbio, SW3015) at room temperature for 1 h. Primary antibodies were applied overnight at 4 °C: COL2A1 (Proteintech, 28459-1-AP, 1:200), SOX9 (HUABIO, HA723548, 1:1000), MMP9 (HUABIO, ET1704-69, 1:200), and ADAMTS5 (HUABIO, HA722011, 1:100). .. After three PBS washes, fluorescent secondary antibodies (Servicebio, Alexa Fluor 488, GB25303; Alexa Fluor 594, GB28303) were incubated for 1 h in the dark, nuclei were counterstained with DAPI (1 μg mL −1 , 5 min, Servicebio, G1012), and images were acquired on a Nikon Article Title: circOMA1 delivered by exosomes regulates DRD2-mediated prolactinoma resistance Article Snippet: The circOMA1 hybridisation probe was designed and synthesised by Shanghai GenePharma, Co., Ltd. RNA-FISH was performed on circOM cells and tissues using an in-situ hybridisation kit (Exon Biotech Inc.), according to the manufacturer's protocol. .. Images were obtained on a Nikon Fluorescence:Article Title: Sustained release PLGA microspheres loaded with a bone-affinity Bmp2 enhance fracture healing and mitigate heterotopic ossification Article Snippet: The samples were then incubated with a Cy3-conjugated secondary antibody (Servicebio, China) for 50 min. .. Images were captured using an Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ), cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h, washed twice with PBS, and incubated with JC-1 working solution (Beyotime, C2006) at 37 °C for 30 min in the dark. .. Following three PBS washes, red (aggregates) and green (monomers) fluorescence were acquired on a Confocal:Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ), cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h, washed twice with PBS, and incubated with JC-1 working solution (Beyotime, C2006) at 37 °C for 30 min in the dark. .. Following three PBS washes, red (aggregates) and green (monomers) fluorescence were acquired on a Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: Rat chondrocytes (P1) were seeded in 24-well plates (5 × 10 4 cells per well) and cultured to ∼70–80% confluence, stimulated with LPS (10 μg mL −1 , 12 h) to induce an inflammatory phenotype, and then treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were washed three times with PBS, fixed in 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X-100 (Aladdin, T434386) for 10 min, and blocked with 5% BSA (Solarbio, SW3015) at room temperature for 1 h. Primary antibodies were applied overnight at 4 °C: COL2A1 (Proteintech, 28459-1-AP, 1:200), SOX9 (HUABIO, HA723548, 1:1000), MMP9 (HUABIO, ET1704-69, 1:200), and ADAMTS5 (HUABIO, HA722011, 1:100). .. After three PBS washes, fluorescent secondary antibodies (Servicebio, Alexa Fluor 488, GB25303; Alexa Fluor 594, GB28303) were incubated for 1 h in the dark, nuclei were counterstained with DAPI (1 μg mL −1 , 5 min, Servicebio, G1012), and images were acquired on a Nikon Membrane:Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ), cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h, washed twice with PBS, and incubated with JC-1 working solution (Beyotime, C2006) at 37 °C for 30 min in the dark. .. Following three PBS washes, red (aggregates) and green (monomers) fluorescence were acquired on a Incubation:Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7 Article Snippet: To visualize biofilm formation and exopolysaccharide production, cultures were stained with Calcofluor to a final concentration of 85 μM, and 1 mM of propidium iodide (PI) (Sigma Aldrich, United States). .. After five days of static incubation at 30 °C, three-dimensional biofilm structures were observed using an Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: Rat chondrocytes (P1) were seeded in 24-well plates (5 × 10 4 cells per well) and cultured to ∼70–80% confluence, stimulated with LPS (10 μg mL −1 , 12 h) to induce an inflammatory phenotype, and then treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were washed three times with PBS, fixed in 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X-100 (Aladdin, T434386) for 10 min, and blocked with 5% BSA (Solarbio, SW3015) at room temperature for 1 h. Primary antibodies were applied overnight at 4 °C: COL2A1 (Proteintech, 28459-1-AP, 1:200), SOX9 (HUABIO, HA723548, 1:1000), MMP9 (HUABIO, ET1704-69, 1:200), and ADAMTS5 (HUABIO, HA722011, 1:100). .. After three PBS washes, fluorescent secondary antibodies (Servicebio, Alexa Fluor 488, GB25303; Alexa Fluor 594, GB28303) were incubated for 1 h in the dark, nuclei were counterstained with DAPI (1 μg mL −1 , 5 min, Servicebio, G1012), and images were acquired on a Nikon Laser-Scanning Microscopy:Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7 Article Snippet: To visualize biofilm formation and exopolysaccharide production, cultures were stained with Calcofluor to a final concentration of 85 μM, and 1 mM of propidium iodide (PI) (Sigma Aldrich, United States). .. After five days of static incubation at 30 °C, three-dimensional biofilm structures were observed using an Imaging:Article Title: Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptake Article Snippet: .. Confocal imaging was performed using a laser scanning confocal microscope (NIKON Eclipse Ti) equipped with the |