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flowcomp™ release buffer  (Thermo Fisher)


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    Thermo Fisher flowcomp™ release buffer
    Flowcomp™ Release Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flowcomp+release+buffer/pmc11544820-82-12-15
    Average 90 stars, based on 1 article reviews
    flowcomp™ release buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Cannabidiol (CBD) Protects Lung Endothelial Cells from Irradiation-Induced Oxidative Stress and Inflammation In Vitro and In Vivo
    Article Snippet: CD31 + cells were then detached from the beads by incubating with FlowCompTM Release buffer (Invitrogen) and separated from the beads via a magnet, after which they were cultured, as described above.

    Article Title: Chronic inflammatory effects of in vivo irradiation of the murine heart on endothelial cells mimic mechanisms involved in atherosclerosis
    Article Snippet: CD31-positive cells were isolated using a magnetic bead separation method (InvitrogenTM DynaMagTM magnet), after which CD31-positive cells immobilized to the DSB-X–streptavidin bead complex were released using biotin–streptavidin competition by incubating beads in 1 ml FlowCompTM release buffer (Invitrogen) for 2 min at 21 °C and pipetting 10 times.

    Cell Isolation:

    Article Title: On-chip magnetophoretic isolation of CD4 + T cells from blood
    Article Snippet: .. After cell isolation, magnetic beads were detached from the cells using a FlowComp Release Buffer (Invitrogen Corporation) by incubation and rotation for 10 min at room temperature. .. The isolated target collections were then analyzed on a FACSAria (BD Biosciences) flow cytometer.

    Magnetic Beads:

    Article Title: On-chip magnetophoretic isolation of CD4 + T cells from blood
    Article Snippet: .. After cell isolation, magnetic beads were detached from the cells using a FlowComp Release Buffer (Invitrogen Corporation) by incubation and rotation for 10 min at room temperature. .. The isolated target collections were then analyzed on a FACSAria (BD Biosciences) flow cytometer.

    Incubation:

    Article Title: On-chip magnetophoretic isolation of CD4 + T cells from blood
    Article Snippet: .. After cell isolation, magnetic beads were detached from the cells using a FlowComp Release Buffer (Invitrogen Corporation) by incubation and rotation for 10 min at room temperature. .. The isolated target collections were then analyzed on a FACSAria (BD Biosciences) flow cytometer.

    Article Title: Adhesion Molecule Expression and Function of Primary Endothelial Cells in Benign and Malignant Tissues Correlates with Proliferation
    Article Snippet: CD31-positive cells immobilized in the DSB-X-streptavidin-bead-complex, remained on the wall of the tube due to the magnetic field (magnet, Invitrogen). .. After at least 5 washing steps with isolation buffer (1 ml), cells attached to beads were resuspended in 1 ml FlowComp release buffer (Invitrogen) and incubated for 2 minutes at 21°C. ..

    Article Title: In vivo Evaluation of Human Embryonic Stem Cells Isolated by 57-C11 Monoclonal Antibody
    Article Snippet: .. Cells were then removed from the magnet by incubation of 1 ml of FlowComp Release Buffer (Life Technologies) for 10 min at room temperature (RT) with rolling and tilting. ..

    Isolation:

    Article Title: Adhesion Molecule Expression and Function of Primary Endothelial Cells in Benign and Malignant Tissues Correlates with Proliferation
    Article Snippet: CD31-positive cells immobilized in the DSB-X-streptavidin-bead-complex, remained on the wall of the tube due to the magnetic field (magnet, Invitrogen). .. After at least 5 washing steps with isolation buffer (1 ml), cells attached to beads were resuspended in 1 ml FlowComp release buffer (Invitrogen) and incubated for 2 minutes at 21°C. ..



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    flowcomp release buffer flowcomp flexi kit 11061d  (Thermo Fisher)
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    Characterization of bead–antibody binding. (A) Magnetic bead fluorescence intensity curves generated by varying densities of a fluorescently labeled anti-EpCAM antibody on beads. A 50% maximal binding capacity for each bead type was then identified ( Table S2 ). Dots represent the average of three technical replicates consisting of 100 beads each (300 beads total); error bars represent the standard deviation of the analyzed population. (B) Impact of the bead’s antibody density on target capture. The 50% maximal antibody binding density for M-280s and FlowComps is denoted by their respective vertical dashed line. Points represent the average of three technical replicates; error bars represent the standard deviation; * denotes p < 0.05, which in this case applies to both M-280 and <t>FlowComp.</t>
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    1 ml of flowcomp release buffer  (Thermo Fisher)
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    Thermo Fisher 1 ml of flowcomp release buffer
    Characterization of bead–antibody binding. (A) Magnetic bead fluorescence intensity curves generated by varying densities of a fluorescently labeled anti-EpCAM antibody on beads. A 50% maximal binding capacity for each bead type was then identified ( Table S2 ). Dots represent the average of three technical replicates consisting of 100 beads each (300 beads total); error bars represent the standard deviation of the analyzed population. (B) Impact of the bead’s antibody density on target capture. The 50% maximal antibody binding density for M-280s and FlowComps is denoted by their respective vertical dashed line. Points represent the average of three technical replicates; error bars represent the standard deviation; * denotes p < 0.05, which in this case applies to both M-280 and <t>FlowComp.</t>
    1 Ml Of Flowcomp Release Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Characterization of bead–antibody binding. (A) Magnetic bead fluorescence intensity curves generated by varying densities of a fluorescently labeled anti-EpCAM antibody on beads. A 50% maximal binding capacity for each bead type was then identified ( Table S2 ). Dots represent the average of three technical replicates consisting of 100 beads each (300 beads total); error bars represent the standard deviation of the analyzed population. (B) Impact of the bead’s antibody density on target capture. The 50% maximal antibody binding density for M-280s and FlowComps is denoted by their respective vertical dashed line. Points represent the average of three technical replicates; error bars represent the standard deviation; * denotes p < 0.05, which in this case applies to both M-280 and FlowComp.

    Journal: ACS Omega

    Article Title: Integration of Magnetic Bead-Based Cell Selection into Complex Isolations

    doi: 10.1021/acsomega.7b01427

    Figure Lengend Snippet: Characterization of bead–antibody binding. (A) Magnetic bead fluorescence intensity curves generated by varying densities of a fluorescently labeled anti-EpCAM antibody on beads. A 50% maximal binding capacity for each bead type was then identified ( Table S2 ). Dots represent the average of three technical replicates consisting of 100 beads each (300 beads total); error bars represent the standard deviation of the analyzed population. (B) Impact of the bead’s antibody density on target capture. The 50% maximal antibody binding density for M-280s and FlowComps is denoted by their respective vertical dashed line. Points represent the average of three technical replicates; error bars represent the standard deviation; * denotes p < 0.05, which in this case applies to both M-280 and FlowComp.

    Article Snippet: For experiments involving release, a similar experimental design was followed, except that once washed, the beads and bound cells were dropped into a release well containing either FlowComp Release Buffer (FlowComp Flexi Kit 11061D, Thermo Fisher) or CELLection release buffer (CELLection Biotin Binder Kit 11533D, Thermo Fisher) prepared according to the manufacturer’s instructions (release volume of 110 μL).

    Techniques: Binding Assay, Fluorescence, Generated, Labeling, Standard Deviation

    Characterization of cell capture. (A) Capture of EpCAM-expressing cell lines (Du145 = low, 22Rv1 = medium, LNCaP = high) by each bead type. Beads are abbreviated as follows: SM = Sera-Mag, FC = FlowComp, CELL = CELLection. (B) Nonspecific capture of PBMCs by each bead type across varying PBMC inputs. (C) Direct vs indirect capture of Du145s from a PBMC background. (D) Resultant purity of the captured target cells from direct and indirect capture of Du145s. In all plots, the bars represent the technical replicate average ( n = 3) with error bars representing the standard deviation.

    Journal: ACS Omega

    Article Title: Integration of Magnetic Bead-Based Cell Selection into Complex Isolations

    doi: 10.1021/acsomega.7b01427

    Figure Lengend Snippet: Characterization of cell capture. (A) Capture of EpCAM-expressing cell lines (Du145 = low, 22Rv1 = medium, LNCaP = high) by each bead type. Beads are abbreviated as follows: SM = Sera-Mag, FC = FlowComp, CELL = CELLection. (B) Nonspecific capture of PBMCs by each bead type across varying PBMC inputs. (C) Direct vs indirect capture of Du145s from a PBMC background. (D) Resultant purity of the captured target cells from direct and indirect capture of Du145s. In all plots, the bars represent the technical replicate average ( n = 3) with error bars representing the standard deviation.

    Article Snippet: For experiments involving release, a similar experimental design was followed, except that once washed, the beads and bound cells were dropped into a release well containing either FlowComp Release Buffer (FlowComp Flexi Kit 11061D, Thermo Fisher) or CELLection release buffer (CELLection Biotin Binder Kit 11533D, Thermo Fisher) prepared according to the manufacturer’s instructions (release volume of 110 μL).

    Techniques: Expressing, Standard Deviation

    Characterization of the release from FlowComp and CELLection beads. (A,B) Release of a fluorescently labeled anti-EpCAM antibody from (A) FlowComp and (B) CELLection beads. Beads were labeled with low, medium, and high levels of antibody and released for the specified time intervals. (A,B) Dots represent the average of three technical replicates with each technical replicate representing the average of 100 beads (total of 300 beads); error bars represent the standard deviation of the technical replicates. (C) Release of 22Rv1s from FlowComp and CELLection beads across time. (D) Capture efficiency of both FlowComp and CELLection beads when used to capture Du145, 22Rv1, and LNCaP cells. (E) Release efficiency of the three cell lines following bead-based capture. (F) Effective capture following the release of the cells. Gray bars represent the population of cells lost during the release process because of the inefficient release. In each plot, bars represent an average of n = 3; error bars represent the standard deviation.

    Journal: ACS Omega

    Article Title: Integration of Magnetic Bead-Based Cell Selection into Complex Isolations

    doi: 10.1021/acsomega.7b01427

    Figure Lengend Snippet: Characterization of the release from FlowComp and CELLection beads. (A,B) Release of a fluorescently labeled anti-EpCAM antibody from (A) FlowComp and (B) CELLection beads. Beads were labeled with low, medium, and high levels of antibody and released for the specified time intervals. (A,B) Dots represent the average of three technical replicates with each technical replicate representing the average of 100 beads (total of 300 beads); error bars represent the standard deviation of the technical replicates. (C) Release of 22Rv1s from FlowComp and CELLection beads across time. (D) Capture efficiency of both FlowComp and CELLection beads when used to capture Du145, 22Rv1, and LNCaP cells. (E) Release efficiency of the three cell lines following bead-based capture. (F) Effective capture following the release of the cells. Gray bars represent the population of cells lost during the release process because of the inefficient release. In each plot, bars represent an average of n = 3; error bars represent the standard deviation.

    Article Snippet: For experiments involving release, a similar experimental design was followed, except that once washed, the beads and bound cells were dropped into a release well containing either FlowComp Release Buffer (FlowComp Flexi Kit 11061D, Thermo Fisher) or CELLection release buffer (CELLection Biotin Binder Kit 11533D, Thermo Fisher) prepared according to the manufacturer’s instructions (release volume of 110 μL).

    Techniques: Labeling, Standard Deviation

    Cell viability following capture and release. (A) The viability of cells (LNCaP, 22Rv1) bound to nonreleaseable beads compared to untouched cells (underwent no magnetic bead isolation) following a 3-day culture (abbreviations: SM = Sera-Mag). (B) The viability of cells bound to (bound) and released from (released) releasable beads (CELLection, FlowComp) following a 3-day culture. Bars represent the average of three technical replicates; error bars represent the standard deviation.

    Journal: ACS Omega

    Article Title: Integration of Magnetic Bead-Based Cell Selection into Complex Isolations

    doi: 10.1021/acsomega.7b01427

    Figure Lengend Snippet: Cell viability following capture and release. (A) The viability of cells (LNCaP, 22Rv1) bound to nonreleaseable beads compared to untouched cells (underwent no magnetic bead isolation) following a 3-day culture (abbreviations: SM = Sera-Mag). (B) The viability of cells bound to (bound) and released from (released) releasable beads (CELLection, FlowComp) following a 3-day culture. Bars represent the average of three technical replicates; error bars represent the standard deviation.

    Article Snippet: For experiments involving release, a similar experimental design was followed, except that once washed, the beads and bound cells were dropped into a release well containing either FlowComp Release Buffer (FlowComp Flexi Kit 11061D, Thermo Fisher) or CELLection release buffer (CELLection Biotin Binder Kit 11533D, Thermo Fisher) prepared according to the manufacturer’s instructions (release volume of 110 μL).

    Techniques: Isolation, Standard Deviation

    Characterization of nucleic acid extraction with cell isolation magnetic beads present. (A,B) Relative fold change in the mRNA transcript (HPRT) detected from LNCaPs. Isolations containing cell isolation beads were compared to no-bead controls for two methods of RNA extraction: (A) spin columns and (B) bead-based extraction. (C,D) Similarly, relative fold change in GAPDH from DNA extracted via (C) spins columns or (D) bead-based extraction. Bars represent the average of three technical replicates; error bars represent the standard deviation; * denotes p < 0.05 and *** denotes p < 0.001; --- indicates the cell only, no-bead control (abbreviations: CELL = CELLection, SM = Sera-Mag, FC = FlowComp).

    Journal: ACS Omega

    Article Title: Integration of Magnetic Bead-Based Cell Selection into Complex Isolations

    doi: 10.1021/acsomega.7b01427

    Figure Lengend Snippet: Characterization of nucleic acid extraction with cell isolation magnetic beads present. (A,B) Relative fold change in the mRNA transcript (HPRT) detected from LNCaPs. Isolations containing cell isolation beads were compared to no-bead controls for two methods of RNA extraction: (A) spin columns and (B) bead-based extraction. (C,D) Similarly, relative fold change in GAPDH from DNA extracted via (C) spins columns or (D) bead-based extraction. Bars represent the average of three technical replicates; error bars represent the standard deviation; * denotes p < 0.05 and *** denotes p < 0.001; --- indicates the cell only, no-bead control (abbreviations: CELL = CELLection, SM = Sera-Mag, FC = FlowComp).

    Article Snippet: For experiments involving release, a similar experimental design was followed, except that once washed, the beads and bound cells were dropped into a release well containing either FlowComp Release Buffer (FlowComp Flexi Kit 11061D, Thermo Fisher) or CELLection release buffer (CELLection Biotin Binder Kit 11533D, Thermo Fisher) prepared according to the manufacturer’s instructions (release volume of 110 μL).

    Techniques: Extraction, Cell Isolation, Magnetic Beads, RNA Extraction, Standard Deviation, Control