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Becker & Hickl spc-150n tcspc/flim module
Spc 150n Tcspc/Flim Module, supplied by Becker & Hickl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flim+module/tcspc+card/pmc11440180-40-21-26
Average 90 stars, based on 1 article reviews
spc-150n tcspc/flim module - by Bioz Stars, 2026-11
90/100 stars

Images

Related Articles

other:

Article Title: Pathological mutation in SMN impairs modulation of GAR1 phase separation linking condensate dysfunction to Spinal Muscular Atrophy
Article Snippet: This combined an 80 MHz, near-infrared, femtosecond excitation source (Insight X3, Spectra Physics, Crewe, UK), second harmonic generation unit (Harmonixx SHG, APE, Berlin, Germany), DCS-120 laser scanning unit (Becker & Hickl, Berlin, Germany), inverted microscope (Axio Observer 7, Zeiss, Cambridge, UK) with high (1.4) numerical aperture objective (Plan-Apochromat 63x/1.4 Oil M27, Zeiss, Cambridge, UK), two ultrafast hybrid detectors (HPM-100-07, Becker & Hickl, Berlin, Germany) and time-correlated single photon counting (TCSPC) electronics (SPC-180NX, Becker & Hickl, Berlin, Germany).

Article Title: Redox-dependent binding and conformational equilibria govern the fluorescence decay of NAD(P)H in living cells
Article Snippet: This combined an 80MHz, near-infrared, femtosecond excitation source (Insight X3, Spectra Physics, Crewe, UK), DCS-120 laser scanning unit (Becker & Hickl, Berlin, Germany), inverted microscope (Axio Observer 7, Zeiss, Cambridge, UK) with high (1.4) numerical aperture objective (Plan-Apochromat 63x/1.4 Oil M27, Zeiss, Cambridge, UK), two ultrafast hybrid detectors (HPM-100-07, Becker & Hickl, Berlin, Germany) and time-correlated single photon counting (TCSPC) electronics (SPC-180NX, Becker & Hickl, Berlin, Germany).

Article Title: Simultaneous assessment of NAD(P)H and flavins with multispectral fluorescence lifetime imaging microscopy at a single excitation wavelength of 750 nm
Article Snippet: – An LSM 880 NLO multiphoton laser scanning microscope (Carl Zeiss, Germany) was equipped with a PML-SPEC-GaAsP multi-wavelength detector and an SPC-150N TCSPC/FLIM module both from Becker & Hickl GmbH, Germany.

Article Title: Evidence for a compact σ 70 conformation in vitro and in vivo
Article Snippet: Single fluorescence photons are then detected using hybrid PMTs (Model R10467U-40, Hamamatsu, Japan), and single photon detection event time tags are collected using a time-correlated single-photon counting card (SPC 150N, Becker & Hickl, GmbH).

Article Title: PEG-mCherry interactions beyond classical macromolecular crowding
Article Snippet: Fluorescence was collected using one hybrid PMT, routed to a TCSPC card (SPC 150N, Becker & Hickl, GmbH) with a TAC window of 12.5 ns.

Article Title: Bimodal functionality of highly conductive nanostructured silver film towards improved performance of photosystem I-based graphene photocathode.
Article Snippet: Emitted light was filtered by the combination of two optical filters (HQ6555LP and HQ675/20, Chroma) and detected by a fast avalanche photodiode (id 100–50, idQuantique) combined with a SPC-150 measuring module (Becker&Hickl).

Article Title: Fluorescence Lifetime Imaging of NAD(P)H in Patients’ Lymphocytes: Evaluation of Efficacy of Immunotherapy
Article Snippet: Briefly, the imaging was performed using a laser scanning microscope Zeiss LSM 880 (Carl Zeiss, Jena, Germany) equipped with a fs laser (Spectra-Physics, Milpitas, CA, USA) and a single photon counting card SPC-150N, (Becker&Hickl GmbH, Berlin, Germany) with a hybrid detector HPM-100-40 (Becker&Hickl GmbH, Berlin, Germany).

Fluorescence:

Article Title: Functionalized Nanodiamonds for Targeted Neuronal Electromagnetic Signal Detection
Article Snippet: .. Furthermore, fluorescence lifetime experiments were performed on a custom-built fluorescence confocal setup where the acquisition was done via a single-photon counting avalanche photodiode connected to a Becker & Hickl time-correlated single photon counting card (TCSPC-150N). .. The laser utilized was a 561 nm laser (BDS-561-SMY Becker & Hickl), with a repetition rate set to 20 MHz and 14 μW of laser power measured at the back aperture of the microscope objective.



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Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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Becker & Hickl tcspc flim module
Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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Becker & Hickl spc-150n tcspc/flim module
Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
Spc 150n Tcspc/Flim Module, supplied by Becker & Hickl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becker & Hickl flim module spc 150 tcspc
Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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Image Search Results


Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative FLIM images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

Journal: Biomedicines

Article Title: Unraveling Microviscosity Changes Induced in Cancer Cells by Photodynamic Therapy with Targeted Genetically Encoded Photosensitizer

doi: 10.3390/biomedicines12112550

Figure Lengend Snippet: Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative FLIM images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

Article Snippet: For viscous imaging, a LSM 880 laser scanning microscope (Carl Zeiss, Gottingen, Germany) equipped with a FLIM SPC 150 TCSPC module (Becker & Hickl GmbH, Berlin, Germany) and a Mai Tai HP femtosecond laser (80 MHz, 140 fs, Spectra Physics, Milpitas, CA, USA) were used.

Techniques: Membrane, Viscosity, Control

Plasma membrane microviscosity in HeLa tumor spheroids after PDT with KillerRed localized in the nuclei (H2B) or within the plasma membrane (PM). ( A ) Schematic representation of the spheroid area (shown by the yellow square) imaged by FLIM. The spheroid had adhered to the glass bottom, and the images were acquired from a depth of ~30 μm. Higher-magnification image of the molecular rotor distribution in spheroid cell membranes indicated by the red squares. The scale bar is 80 μm. ( B ) FLIM images and live/dead (LD) assay of control and treated cells in spheroids. Bar = 80 μm. ( C ) Morphology of control and treated spheroids. The scale bar is 80 μm. ( D ) Quantification of membrane microviscosity of spheroid cells after PDT. Means ± SD, n = 4 spheroids, 60 cells in each. ( E ) Quantitative analysis of dead cells in control and treated cell populations, %. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

Journal: Biomedicines

Article Title: Unraveling Microviscosity Changes Induced in Cancer Cells by Photodynamic Therapy with Targeted Genetically Encoded Photosensitizer

doi: 10.3390/biomedicines12112550

Figure Lengend Snippet: Plasma membrane microviscosity in HeLa tumor spheroids after PDT with KillerRed localized in the nuclei (H2B) or within the plasma membrane (PM). ( A ) Schematic representation of the spheroid area (shown by the yellow square) imaged by FLIM. The spheroid had adhered to the glass bottom, and the images were acquired from a depth of ~30 μm. Higher-magnification image of the molecular rotor distribution in spheroid cell membranes indicated by the red squares. The scale bar is 80 μm. ( B ) FLIM images and live/dead (LD) assay of control and treated cells in spheroids. Bar = 80 μm. ( C ) Morphology of control and treated spheroids. The scale bar is 80 μm. ( D ) Quantification of membrane microviscosity of spheroid cells after PDT. Means ± SD, n = 4 spheroids, 60 cells in each. ( E ) Quantitative analysis of dead cells in control and treated cell populations, %. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

Article Snippet: For viscous imaging, a LSM 880 laser scanning microscope (Carl Zeiss, Gottingen, Germany) equipped with a FLIM SPC 150 TCSPC module (Becker & Hickl GmbH, Berlin, Germany) and a Mai Tai HP femtosecond laser (80 MHz, 140 fs, Spectra Physics, Milpitas, CA, USA) were used.

Techniques: Membrane, Control