flim module (Becker & Hickl)
90
Structured Review
Becker & Hickl
flim module
Flim Module, supplied by Becker & Hickl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flim+module/flim+module/pmc06780561-83-39-45
Average 90 stars, based on 1 article reviews
Flim Module, supplied by Becker & Hickl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flim+module/flim+module/pmc06780561-83-39-45
Average 90 stars, based on 1 article reviews
flim module - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Multimodal Nonlinear Microscopy for Therapy Monitoring of Cold Atmospheric Plasma Treatment"
Article Title: Multimodal Nonlinear Microscopy for Therapy Monitoring of Cold Atmospheric Plasma Treatment
Journal: Micromachines
doi: 10.3390/mi10090564
Figure Legend Snippet: Photographs of kINPen-device ® ( a ) and endoscopic plasma device ( b ) during operation; ( c ) Sketch of treatment of a thin tissue section using the kINPen-device ® as shown in ( a ). The plasma covers the whole area of the tissue sections under investigation, approximately 3 × 3 mm² in size. ( d ) The plasma jet of the endoscopic plasma device shown in panel ( b ) is moved across the area of the specimen for treatment of the full section. ( e ) Scheme of the setup used for multimodal nonlinear microscopy combining coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG) and two-photon fluorescence lifetime imaging (2P-FLIM). The picosecond (ps) pulse trains of the Ti: sapphire laser/optical parametric oscillator (OPO) system (1) is coupled into the laser scanning microscope (2). The laser light is focused onto the sample by a microscope objective (5) for image acquisition by scanning the specimen (6). Two-photon excited autofluorescence (TPEF) signals are collected by the objective (5) and reflected to the 2P-FLIM detector (4) by a 600 nm short pass dichroic mirror (3). The TP-FLIM signal is filtered from residual laser light by a 650 nm short pass filter and a 458/64 nm bandpass filter (both Semrock, Rochester, MN, USA). The CARS and SHG signals from the sample are collected in a forward direction by a condenser (7), split by a 514 nm long pass dichroic mirror and detected by photomultiplier tube (PMT) modules (9 SHG, 10 CARS) after spectral filtering as described in .
Techniques Used: Microscopy, Fluorescence, Imaging, Laser-Scanning Microscopy
Figure Legend Snippet: Results from two-photon time correlated single photon counting (TCSPC) fluorescence measurements ( a ) The relative change of the total fluorescence (two-photon excitation at 672.5 nm and 832.2 nm, corresponding to single photon excitation at 336 and 416 nm, fluorescence emission at 426–490 nm) after CAP treatment is plotted for all samples normalized to the total fluorescence of the control (=1.0), i.e., all measurements and experimental conditions (treatment time, plasma source, gas composition, specimen), the two plasma devices kINPen MED ® (using Ar and an Ar–oxygen gas mixture for operation and treatment times from 10–70 s) and the endoscopic plasma device (using Ne and a Ne–oxygen gas mixture for operation and treatment times of 10 and 60 s), treatment times of 10 s (in combination with different CAP devices and gas mixtures), longer treatment times (20 s, 60 s and 70 s) and different gas mixtures. Here, Ar and Ar–oxygen gas mixtures were used with the kINPen-device only, Ne and Ne–oxygen gas mixtures were used with the endoscopic plasma device only. ( b ) Change of the median fluorescence lifetime for the parameters of ( a ) for all samples under investigation and all 12 experimental conditions using a monoexponential decay function and plotting the median lifetime of the whole FLIM image. The experimental conditions are in detail: 1,2: Skin, 10 s kINPen, Ar gas; 3: Mucosa lesion, 10 s kINPen, Ar gas; 4: Mucosa, 10 s kINPen, Ar gas; 5,6: Skin, 10 s kINPen, Ar–oxygen gas mixture; 7: Skin, 20 s kINPen, Ar–oxygen gas mixture; 8: Skin, 70 s kINPen, Ar gas; 9: Skin, 10 s endoscopic plasma source, Ne gas; 10,11: Skin, 1 min endoscopic plasma source, Ne gas; 12: Skin, 1 min endoscopic plasma source, Ne–oxygen gas. In eight cases a lifetime increase was observed, while in four cases the fluorescence lifetime was reduced.
Techniques Used: Fluorescence
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