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flim module  (PicoQuant inc)

 
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    Structured Review

    PicoQuant inc flim module
    ( a ) 3D scatter plot of three quantitative tissue indicators: the redox <t>ratio,</t> <t>fluorescence</t> lifetime, and SHG signal averaged for each tissue subgroup with a Gaussian ellipsoid fit, ( b ) box plot of the average lifetimes, ( c ) bar graph of the redox ratios for each tissue type with the errors corresponding to the standard deviations across all measurements, and ( d ) overlaid TPEF and SHG intensity images.
    Flim Module, supplied by PicoQuant inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flim+module/flim+module/pmc06173695-220-4-7
    Average 90 stars, based on 1 article reviews
    flim module - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Real-time Brain Tumor imaging with endogenous fluorophores: a diagnosis proof-of-concept study on fresh human samples"

    Article Title: Real-time Brain Tumor imaging with endogenous fluorophores: a diagnosis proof-of-concept study on fresh human samples

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-33134-2

    ( a ) 3D scatter plot of three quantitative tissue indicators: the redox ratio, fluorescence lifetime, and SHG signal averaged for each tissue subgroup with a Gaussian ellipsoid fit, ( b ) box plot of the average lifetimes, ( c ) bar graph of the redox ratios for each tissue type with the errors corresponding to the standard deviations across all measurements, and ( d ) overlaid TPEF and SHG intensity images.
    Figure Legend Snippet: ( a ) 3D scatter plot of three quantitative tissue indicators: the redox ratio, fluorescence lifetime, and SHG signal averaged for each tissue subgroup with a Gaussian ellipsoid fit, ( b ) box plot of the average lifetimes, ( c ) bar graph of the redox ratios for each tissue type with the errors corresponding to the standard deviations across all measurements, and ( d ) overlaid TPEF and SHG intensity images.

    Techniques Used: Fluorescence



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    Image Search Results


    Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative FLIM images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

    Journal: Biomedicines

    Article Title: Unraveling Microviscosity Changes Induced in Cancer Cells by Photodynamic Therapy with Targeted Genetically Encoded Photosensitizer

    doi: 10.3390/biomedicines12112550

    Figure Lengend Snippet: Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative FLIM images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

    Article Snippet: For viscous imaging, a LSM 880 laser scanning microscope (Carl Zeiss, Gottingen, Germany) equipped with a FLIM SPC 150 TCSPC module (Becker & Hickl GmbH, Berlin, Germany) and a Mai Tai HP femtosecond laser (80 MHz, 140 fs, Spectra Physics, Milpitas, CA, USA) were used.

    Techniques: Membrane, Viscosity, Control

    Plasma membrane microviscosity in HeLa tumor spheroids after PDT with KillerRed localized in the nuclei (H2B) or within the plasma membrane (PM). ( A ) Schematic representation of the spheroid area (shown by the yellow square) imaged by FLIM. The spheroid had adhered to the glass bottom, and the images were acquired from a depth of ~30 μm. Higher-magnification image of the molecular rotor distribution in spheroid cell membranes indicated by the red squares. The scale bar is 80 μm. ( B ) FLIM images and live/dead (LD) assay of control and treated cells in spheroids. Bar = 80 μm. ( C ) Morphology of control and treated spheroids. The scale bar is 80 μm. ( D ) Quantification of membrane microviscosity of spheroid cells after PDT. Means ± SD, n = 4 spheroids, 60 cells in each. ( E ) Quantitative analysis of dead cells in control and treated cell populations, %. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

    Journal: Biomedicines

    Article Title: Unraveling Microviscosity Changes Induced in Cancer Cells by Photodynamic Therapy with Targeted Genetically Encoded Photosensitizer

    doi: 10.3390/biomedicines12112550

    Figure Lengend Snippet: Plasma membrane microviscosity in HeLa tumor spheroids after PDT with KillerRed localized in the nuclei (H2B) or within the plasma membrane (PM). ( A ) Schematic representation of the spheroid area (shown by the yellow square) imaged by FLIM. The spheroid had adhered to the glass bottom, and the images were acquired from a depth of ~30 μm. Higher-magnification image of the molecular rotor distribution in spheroid cell membranes indicated by the red squares. The scale bar is 80 μm. ( B ) FLIM images and live/dead (LD) assay of control and treated cells in spheroids. Bar = 80 μm. ( C ) Morphology of control and treated spheroids. The scale bar is 80 μm. ( D ) Quantification of membrane microviscosity of spheroid cells after PDT. Means ± SD, n = 4 spheroids, 60 cells in each. ( E ) Quantitative analysis of dead cells in control and treated cell populations, %. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

    Article Snippet: For viscous imaging, a LSM 880 laser scanning microscope (Carl Zeiss, Gottingen, Germany) equipped with a FLIM SPC 150 TCSPC module (Becker & Hickl GmbH, Berlin, Germany) and a Mai Tai HP femtosecond laser (80 MHz, 140 fs, Spectra Physics, Milpitas, CA, USA) were used.

    Techniques: Membrane, Control