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spc-830 time-correlated single-photon counting flim module  (Becker & Hickl)

 
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    Structured Review

    Becker & Hickl spc-830 time-correlated single-photon counting flim module
    Spc 830 Time Correlated Single Photon Counting Flim Module, supplied by Becker & Hickl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flim+module/tcspc+module/pm39404364-71-30-36
    Average 90 stars, based on 1 article reviews
    spc-830 time-correlated single-photon counting flim module - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Endonuclease G promotes hepatic mitochondrial respiration by selectively increasing mitochondrial tRNA Thr production.
    Article Snippet: The filtered signals were focused on a PMC100 cooled detector and the electrical pulse output from the detector was directed into an SPC- 150 photon counting card (both from Becker & Hickl GmbH, Berlin, Germany).

    Article Title: Lipophilic molecular rotor to assess the viscosity of oil core in nano-emulsion droplets.
    Article Snippet: The single-photon events were detected with a micro-channel plate photomultiplier R3809U Hamamatsu, coupled with a pulse preamplifier HFAC (Becker-Hickl GmbH) and recorded on a time-correlated single photon counting board SPC-130 (Becker-Hickl GmbH).

    Article Title: Cellular damage triggers mechano-chemical control of cell wall dynamics and patterned cell divisions in plant healing.
    Article Snippet: FLIM images were recorded using a Leica TCS SP8 inverted scanning confocal microscope coupled with a Becker-Hickl SPC830 time-correlated single photon counting (TCSPC) module.

    Article Title: Deep learning model utilizing fluorescence lifetime imaging microscopy and confidence learning for predicting endometrial cancer risk
    Article Snippet: This study introduces a novel diagnostic approach for the prediction of endometrial cancer risk, which involves minimally invasive exfoliation cytology specimens from 63 participants, staining with the viscosity-sensitive fluorescent probe DCVJ, and detection by fluorescence lifetime imaging microscopy (FLIM).. Initially, DCVJstained tissue sections from cancerous and normal regions were utilized to confirm the elevated viscosity in cancerous lesions.. Subsequently, DCVJ-stained aspirated cell samples were detected by FLIM to capture viscosity characteristics and cellular morphology.

    Article Title: Endonuclease G promotes hepatic mitochondrial respiration by selectively increasing mitochondrial tRNA Thr production
    Article Snippet: The filtered signals were focused on a PMC100 cooled detector and the electrical pulse output from the detector was directed into an SPC-150 photon counting card (both from Becker & Hickl GmbH, Berlin, Germany).

    Article Title: Multi-timescale map of radiative and nonradiative decay for exciton in Ag/TiO2 nanoparticles
    Article Snippet: In this study, we present a multi-timescale map of radiative and nonradiative decay processes for excitons in Ag/ TiO2 nanoparticles, with a particular focus on the fluorescence lifetime component analysis of exciton states.. Utilizing both steady-state and time-resolved spectroscopy, we meticulously dissect the influence of silver doping on the optical properties and exciton dynamics of TiO2 nanoparticles.. Raman spectroscopy reveals phonon confinement, lattice strain, and non-stoichiometric defects resulting from Ag incorporation, which disrupts the oxygen ion arrangement around Ti4+ and induces oxygen vacancies.

    Article Title: Optical metabolic imaging identifies metabolic shifts and mitochondria heterogeneity in POLG mutator macrophages
    Article Snippet: NAD(P)H and FAD autofluorescence intensity and lifetime images of BMDMs were captured using a customized multiphoton imaging system (Mariana, 3i) coupled with a time-correlated single-photon counting (TCSPC) electronics module (SPC -150N, Becker & Hickl).

    Microscopy:

    Article Title: Endonuclease G promotes hepatic mitochondrial respiration by selectively increasing mitochondrial tRNA Thr production.
    Article Snippet: The filtered signals were focused on a PMC100 cooled detector and the electrical pulse output from the detector was directed into an SPC- 150 photon counting card (both from Becker & Hickl GmbH, Berlin, Germany).

    Article Title: Lipophilic molecular rotor to assess the viscosity of oil core in nano-emulsion droplets.
    Article Snippet: The single-photon events were detected with a micro-channel plate photomultiplier R3809U Hamamatsu, coupled with a pulse preamplifier HFAC (Becker-Hickl GmbH) and recorded on a time-correlated single photon counting board SPC-130 (Becker-Hickl GmbH).

    Article Title: Cellular damage triggers mechano-chemical control of cell wall dynamics and patterned cell divisions in plant healing.
    Article Snippet: FLIM images were recorded using a Leica TCS SP8 inverted scanning confocal microscope coupled with a Becker-Hickl SPC830 time-correlated single photon counting (TCSPC) module.

    Article Title: Deep learning model utilizing fluorescence lifetime imaging microscopy and confidence learning for predicting endometrial cancer risk
    Article Snippet: This study introduces a novel diagnostic approach for the prediction of endometrial cancer risk, which involves minimally invasive exfoliation cytology specimens from 63 participants, staining with the viscosity-sensitive fluorescent probe DCVJ, and detection by fluorescence lifetime imaging microscopy (FLIM).. Initially, DCVJstained tissue sections from cancerous and normal regions were utilized to confirm the elevated viscosity in cancerous lesions.. Subsequently, DCVJ-stained aspirated cell samples were detected by FLIM to capture viscosity characteristics and cellular morphology.

    Article Title: Endonuclease G promotes hepatic mitochondrial respiration by selectively increasing mitochondrial tRNA Thr production
    Article Snippet: The filtered signals were focused on a PMC100 cooled detector and the electrical pulse output from the detector was directed into an SPC-150 photon counting card (both from Becker & Hickl GmbH, Berlin, Germany).

    Article Title: Multi-timescale map of radiative and nonradiative decay for exciton in Ag/TiO2 nanoparticles
    Article Snippet: In this study, we present a multi-timescale map of radiative and nonradiative decay processes for excitons in Ag/ TiO2 nanoparticles, with a particular focus on the fluorescence lifetime component analysis of exciton states.. Utilizing both steady-state and time-resolved spectroscopy, we meticulously dissect the influence of silver doping on the optical properties and exciton dynamics of TiO2 nanoparticles.. Raman spectroscopy reveals phonon confinement, lattice strain, and non-stoichiometric defects resulting from Ag incorporation, which disrupts the oxygen ion arrangement around Ti4+ and induces oxygen vacancies.

    Article Title: Optical metabolic imaging identifies metabolic shifts and mitochondria heterogeneity in POLG mutator macrophages
    Article Snippet: NAD(P)H and FAD autofluorescence intensity and lifetime images of BMDMs were captured using a customized multiphoton imaging system (Mariana, 3i) coupled with a time-correlated single-photon counting (TCSPC) electronics module (SPC -150N, Becker & Hickl).



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    Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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    Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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    Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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    Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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    Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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    Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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    Image Search Results


    Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative FLIM images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

    Journal: Biomedicines

    Article Title: Unraveling Microviscosity Changes Induced in Cancer Cells by Photodynamic Therapy with Targeted Genetically Encoded Photosensitizer

    doi: 10.3390/biomedicines12112550

    Figure Lengend Snippet: Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative FLIM images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

    Article Snippet: For viscous imaging, a LSM 880 laser scanning microscope (Carl Zeiss, Gottingen, Germany) equipped with a FLIM SPC 150 TCSPC module (Becker & Hickl GmbH, Berlin, Germany) and a Mai Tai HP femtosecond laser (80 MHz, 140 fs, Spectra Physics, Milpitas, CA, USA) were used.

    Techniques: Membrane, Viscosity, Control

    Plasma membrane microviscosity in HeLa tumor spheroids after PDT with KillerRed localized in the nuclei (H2B) or within the plasma membrane (PM). ( A ) Schematic representation of the spheroid area (shown by the yellow square) imaged by FLIM. The spheroid had adhered to the glass bottom, and the images were acquired from a depth of ~30 μm. Higher-magnification image of the molecular rotor distribution in spheroid cell membranes indicated by the red squares. The scale bar is 80 μm. ( B ) FLIM images and live/dead (LD) assay of control and treated cells in spheroids. Bar = 80 μm. ( C ) Morphology of control and treated spheroids. The scale bar is 80 μm. ( D ) Quantification of membrane microviscosity of spheroid cells after PDT. Means ± SD, n = 4 spheroids, 60 cells in each. ( E ) Quantitative analysis of dead cells in control and treated cell populations, %. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

    Journal: Biomedicines

    Article Title: Unraveling Microviscosity Changes Induced in Cancer Cells by Photodynamic Therapy with Targeted Genetically Encoded Photosensitizer

    doi: 10.3390/biomedicines12112550

    Figure Lengend Snippet: Plasma membrane microviscosity in HeLa tumor spheroids after PDT with KillerRed localized in the nuclei (H2B) or within the plasma membrane (PM). ( A ) Schematic representation of the spheroid area (shown by the yellow square) imaged by FLIM. The spheroid had adhered to the glass bottom, and the images were acquired from a depth of ~30 μm. Higher-magnification image of the molecular rotor distribution in spheroid cell membranes indicated by the red squares. The scale bar is 80 μm. ( B ) FLIM images and live/dead (LD) assay of control and treated cells in spheroids. Bar = 80 μm. ( C ) Morphology of control and treated spheroids. The scale bar is 80 μm. ( D ) Quantification of membrane microviscosity of spheroid cells after PDT. Means ± SD, n = 4 spheroids, 60 cells in each. ( E ) Quantitative analysis of dead cells in control and treated cell populations, %. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

    Article Snippet: For viscous imaging, a LSM 880 laser scanning microscope (Carl Zeiss, Gottingen, Germany) equipped with a FLIM SPC 150 TCSPC module (Becker & Hickl GmbH, Berlin, Germany) and a Mai Tai HP femtosecond laser (80 MHz, 140 fs, Spectra Physics, Milpitas, CA, USA) were used.

    Techniques: Membrane, Control