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5× first strand buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 5× first strand buffer
    RT mix-II
    5× First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/first+strand+buffer/FSB+buffer/pmc12765176-63-0-11
    Average 96 stars, based on 1 article reviews
    5× first strand buffer - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Protocol to isolate broadly neutralizing monoclonal antibodies against SARS-CoV-2 from human B cells"

    Article Title: Protocol to isolate broadly neutralizing monoclonal antibodies against SARS-CoV-2 from human B cells

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2025.104275


    Figure Legend Snippet: RT mix-II

    Techniques Used:


    Figure Legend Snippet:

    Techniques Used: Virus, Recombinant, Sterility, Saline, Magnetic Beads, Expressing, Reverse Transcription, Cloning, Luciferase, Plasmid Preparation, Purification, DNA Purification, Software, Cell Culture, Binding Assay, Enzyme-linked Immunosorbent Assay, Adhesive

    Related Articles

    Reverse Transcription:

    Article Title: CD13 is a bona-fide marker of bovine pre-adipocytes with potential in cultivated fat applications.
    Article Snippet: RNA (300 ng) was incubated with 0.5 μl random primers (250ng/μl, Promega, 1181), 1 μl dNTPs (10mM, Invitrogen, 18427- 013) and nuclease free-water in a thermocycler (Biometra) at 65 °C for 5 min, followed by 5 min at 4 °C. .. Then, 1 μl DTT (0.1 M, Invitrogen, 18080-93), 1 μl RNasin Plus RNase inhibitor (40 U/μL, Promega, N2611), 1 μl SuperScript III reverse transcriptase (Invitrogen, 18080-93), and 4 μl first strand buffer (5X, Invitrogen, 18080-93) were added and samples incubated at 25 °C for 5 min, followed by 50 °C for 60 min, 70 °C for 15 min and a cooling step at 4 °C for 5 min. No-templateand a no-RT-controls were included. cDNA was kept frozen at –20 °C. .. Duplicate cDNA samples (2 μl, 1/40 dilution) were combined with 5 μl SensiFAST SYBR green (Kit, Meridian Bioscience, BIO94020), forward and reverse primer (10 μM, 0.4 μl each, Supplementary Table 5), and 2.2 μl nuclease-free water, and subjected to 95 °C x 2 min, followed by 40 cycles of 95 °C x 5 sec, 60 °C x 11 sec and 72 °C x 5 sec, and a final incubation at 95 °C x 1 min, 60 °C x 30 sec and 95 °C x 30 sec in a AriaMx Real-time PCR System (Agilent).

    Article Title: Multifaceted B cell response to transient HIV viremia in elite controllers.
    Article Snippet: .. Each well then received 5.4 μL of reverse transcription mix containing Superscript II reverse transcriptase (50 units) (Thermo Fisher), RNAse inhibitor (10 units) (Tarka Bio), 5X first strand buffer (Thermo Fisher), DTT (5mM) (Thermo Fisher), betaine (1M) (Sigma), MgCl2 (12mM) (Sigma Aldrich), Nuclease-free water (Thermo Fisher) and template switching-oligo (5′- AAGCAGTGGTATCAACGCAGAGTACATr GrG + G-3′) (1μM). ..

    Article Title: cxcl18b -defined transitional state-specific nitric oxide drives injury-induced Müller glia cell-cycle re-entry in the zebrafish retina
    Article Snippet: .. Interesting cells were collected into a 1.5 ml tube containing lysis buffer (20 mg/ml PK in TE buffer) and total RNA was isolated using this lysis buffer. cDNA synthesis was conducted by adding RT mix containing: 200 U Superscript II reverse transcriptase (Invitrogen, 18064‐014), 1 × First‐strand buffer (Invitrogen, 18064‐014), 5 mM DTT (Invitrogen, 18064‐014), 20 U Recombinant RNase inhibitor (Clontech, 2313A), 6 mM MgCl 2 (Sigma, M8266), 1 μM TSO ( ); 8% PEG8000 (Sigma, P1458). ..

    Article Title: Altered placental expression of neurotransmitter and stress-related molecules in first-episode psychosis during pregnancy: Implications for cytoarchitecture and function.
    Article Snippet: .. A reverse transcription reaction mix was prepared, containing 2.8 μL of First Strand Buffer 5X (Thermo Fisher Scientific), 1 μL of reverse transcriptase enzyme, 2 μL of 10 mM dNTP mix, 2 μL of 0.1 M DTT, 1.7 μL of nuclease-free water, and 0.5 μL of RNase inhibitor. ..

    Article Title: Impaired function of Vγ9Vδ2 T cells in frail elderly.
    Article Snippet: .. The RT reaction mixture contained 0.5 μL SuperScript II reverse transcriptase (200 U/μL, Invitrogen), 0.25 μL RNase inhibitor (40 U/μL, Clontech), 2 μL 5× SuperScript II First-Strand Buffer (Invitrogen), 0.5 μL DTT (0.1 M, Invitrogen), 2 μL Betaine (5 M, Sigma), 0.06 μL MgCl2 (1 M, Sigma), and 0.1 μL TSO (100 μM final concentration). ..

    Article Title: Protocol to isolate broadly neutralizing monoclonal antibodies against SARS-CoV-2 from human B cells
    Article Snippet: .. 5× First-Strand Buffer (part of the SuperScript III Reverse Transcriptase) , Thermo Fisher Scientific , Cat#18080044. .. SuperScript III Reverse Transcriptase , Thermo Fisher Scientific , Cat#18080044.

    Article Title: Dynamics of natural and pharmacologic control of an SIV variant with an envelope trafficking defect
    Article Snippet: .. To this initial mixture, we added a 25 μl mixture of working concentrations of 1X first-strand buffer, 1 mM DTT, 1 mM RNaseOUT, and 4 U/μl SuperScript III reverse transcriptase (Thermo Fisher Scientific). ..

    Incubation:

    Article Title: CD13 is a bona-fide marker of bovine pre-adipocytes with potential in cultivated fat applications.
    Article Snippet: RNA (300 ng) was incubated with 0.5 μl random primers (250ng/μl, Promega, 1181), 1 μl dNTPs (10mM, Invitrogen, 18427- 013) and nuclease free-water in a thermocycler (Biometra) at 65 °C for 5 min, followed by 5 min at 4 °C. .. Then, 1 μl DTT (0.1 M, Invitrogen, 18080-93), 1 μl RNasin Plus RNase inhibitor (40 U/μL, Promega, N2611), 1 μl SuperScript III reverse transcriptase (Invitrogen, 18080-93), and 4 μl first strand buffer (5X, Invitrogen, 18080-93) were added and samples incubated at 25 °C for 5 min, followed by 50 °C for 60 min, 70 °C for 15 min and a cooling step at 4 °C for 5 min. No-templateand a no-RT-controls were included. cDNA was kept frozen at –20 °C. .. Duplicate cDNA samples (2 μl, 1/40 dilution) were combined with 5 μl SensiFAST SYBR green (Kit, Meridian Bioscience, BIO94020), forward and reverse primer (10 μM, 0.4 μl each, Supplementary Table 5), and 2.2 μl nuclease-free water, and subjected to 95 °C x 2 min, followed by 40 cycles of 95 °C x 5 sec, 60 °C x 11 sec and 72 °C x 5 sec, and a final incubation at 95 °C x 1 min, 60 °C x 30 sec and 95 °C x 30 sec in a AriaMx Real-time PCR System (Agilent).

    Article Title: Compartment-specific transcriptome of motor neurons reveals impaired extracellular matrix signaling and activated cell cycle kinases in FUS-ALS.
    Article Snippet: .. Cells were washed and resuspended in 500 μl FACS staining/buffer solution consisting of 500 μl/sample FxCycleTM PI/RNase staining solution (Thermo Fisher Scientific) according to the manufacturer's instructions and incubated in the dark at 37 ◦C for 30 min. .. Finally, flow cytometry measurements were taken at excitation wavelength of 488 nm using a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA).

    Lysis:

    Article Title: cxcl18b -defined transitional state-specific nitric oxide drives injury-induced Müller glia cell-cycle re-entry in the zebrafish retina
    Article Snippet: .. Interesting cells were collected into a 1.5 ml tube containing lysis buffer (20 mg/ml PK in TE buffer) and total RNA was isolated using this lysis buffer. cDNA synthesis was conducted by adding RT mix containing: 200 U Superscript II reverse transcriptase (Invitrogen, 18064‐014), 1 × First‐strand buffer (Invitrogen, 18064‐014), 5 mM DTT (Invitrogen, 18064‐014), 20 U Recombinant RNase inhibitor (Clontech, 2313A), 6 mM MgCl 2 (Sigma, M8266), 1 μM TSO ( ); 8% PEG8000 (Sigma, P1458). ..

    Isolation:

    Article Title: cxcl18b -defined transitional state-specific nitric oxide drives injury-induced Müller glia cell-cycle re-entry in the zebrafish retina
    Article Snippet: .. Interesting cells were collected into a 1.5 ml tube containing lysis buffer (20 mg/ml PK in TE buffer) and total RNA was isolated using this lysis buffer. cDNA synthesis was conducted by adding RT mix containing: 200 U Superscript II reverse transcriptase (Invitrogen, 18064‐014), 1 × First‐strand buffer (Invitrogen, 18064‐014), 5 mM DTT (Invitrogen, 18064‐014), 20 U Recombinant RNase inhibitor (Clontech, 2313A), 6 mM MgCl 2 (Sigma, M8266), 1 μM TSO ( ); 8% PEG8000 (Sigma, P1458). ..

    cDNA Synthesis:

    Article Title: cxcl18b -defined transitional state-specific nitric oxide drives injury-induced Müller glia cell-cycle re-entry in the zebrafish retina
    Article Snippet: .. Interesting cells were collected into a 1.5 ml tube containing lysis buffer (20 mg/ml PK in TE buffer) and total RNA was isolated using this lysis buffer. cDNA synthesis was conducted by adding RT mix containing: 200 U Superscript II reverse transcriptase (Invitrogen, 18064‐014), 1 × First‐strand buffer (Invitrogen, 18064‐014), 5 mM DTT (Invitrogen, 18064‐014), 20 U Recombinant RNase inhibitor (Clontech, 2313A), 6 mM MgCl 2 (Sigma, M8266), 1 μM TSO ( ); 8% PEG8000 (Sigma, P1458). ..

    Recombinant:

    Article Title: cxcl18b -defined transitional state-specific nitric oxide drives injury-induced Müller glia cell-cycle re-entry in the zebrafish retina
    Article Snippet: .. Interesting cells were collected into a 1.5 ml tube containing lysis buffer (20 mg/ml PK in TE buffer) and total RNA was isolated using this lysis buffer. cDNA synthesis was conducted by adding RT mix containing: 200 U Superscript II reverse transcriptase (Invitrogen, 18064‐014), 1 × First‐strand buffer (Invitrogen, 18064‐014), 5 mM DTT (Invitrogen, 18064‐014), 20 U Recombinant RNase inhibitor (Clontech, 2313A), 6 mM MgCl 2 (Sigma, M8266), 1 μM TSO ( ); 8% PEG8000 (Sigma, P1458). ..

    FACS:

    Article Title: Compartment-specific transcriptome of motor neurons reveals impaired extracellular matrix signaling and activated cell cycle kinases in FUS-ALS.
    Article Snippet: .. Cells were washed and resuspended in 500 μl FACS staining/buffer solution consisting of 500 μl/sample FxCycleTM PI/RNase staining solution (Thermo Fisher Scientific) according to the manufacturer's instructions and incubated in the dark at 37 ◦C for 30 min. .. Finally, flow cytometry measurements were taken at excitation wavelength of 488 nm using a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA).

    Staining:

    Article Title: Compartment-specific transcriptome of motor neurons reveals impaired extracellular matrix signaling and activated cell cycle kinases in FUS-ALS.
    Article Snippet: .. Cells were washed and resuspended in 500 μl FACS staining/buffer solution consisting of 500 μl/sample FxCycleTM PI/RNase staining solution (Thermo Fisher Scientific) according to the manufacturer's instructions and incubated in the dark at 37 ◦C for 30 min. .. Finally, flow cytometry measurements were taken at excitation wavelength of 488 nm using a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA).

    Concentration Assay:

    Article Title: Impaired function of Vγ9Vδ2 T cells in frail elderly.
    Article Snippet: .. The RT reaction mixture contained 0.5 μL SuperScript II reverse transcriptase (200 U/μL, Invitrogen), 0.25 μL RNase inhibitor (40 U/μL, Clontech), 2 μL 5× SuperScript II First-Strand Buffer (Invitrogen), 0.5 μL DTT (0.1 M, Invitrogen), 2 μL Betaine (5 M, Sigma), 0.06 μL MgCl2 (1 M, Sigma), and 0.1 μL TSO (100 μM final concentration). ..



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    Image Search Results


    Journal: STAR Protocols

    Article Title: Protocol to isolate broadly neutralizing monoclonal antibodies against SARS-CoV-2 from human B cells

    doi: 10.1016/j.xpro.2025.104275

    Figure Lengend Snippet: RT mix-II

    Article Snippet: 5× First-Strand Buffer (part of the SuperScript III Reverse Transcriptase) , Thermo Fisher Scientific , Cat#18080044.

    Techniques:

    a Schematic illustration of the SCAN-seq method. Briefly, control and Hnrnpm cKO GV oocytes were lysed to release mRNAs. First-strand cDNAs were synthesized using a 24-nt barcode RT primer. Following amplification, the cDNAs derived from multiple cells were combined, pooled together, and purified. Subsequently, the full-length cDNAs were used for the construction of nanopore libraries and sequencing. The mouse, oocyte, and nucleic acid schematics are modified adaptations of original resources from SciDraw and Servier Medical Art, respectively, and are employed under a CC BY 4.0 license. b – j PCR and Sanger sequencing validation of genes ( Nlrp14, Meikin, Vrk1 , and Zar1l ) with novel isoforms identified in GV oocytes. Ref. Reference. Representative results shown in ( b – e ) were obtained from at least three independent experiments with similar results. Source data ( b – e ) are provided as a Source data file.

    Journal: Nature Communications

    Article Title: hnRNPM cooperates with BCAS2 to modulate alternative splicing during oocyte development

    doi: 10.1038/s41467-026-69176-8

    Figure Lengend Snippet: a Schematic illustration of the SCAN-seq method. Briefly, control and Hnrnpm cKO GV oocytes were lysed to release mRNAs. First-strand cDNAs were synthesized using a 24-nt barcode RT primer. Following amplification, the cDNAs derived from multiple cells were combined, pooled together, and purified. Subsequently, the full-length cDNAs were used for the construction of nanopore libraries and sequencing. The mouse, oocyte, and nucleic acid schematics are modified adaptations of original resources from SciDraw and Servier Medical Art, respectively, and are employed under a CC BY 4.0 license. b – j PCR and Sanger sequencing validation of genes ( Nlrp14, Meikin, Vrk1 , and Zar1l ) with novel isoforms identified in GV oocytes. Ref. Reference. Representative results shown in ( b – e ) were obtained from at least three independent experiments with similar results. Source data ( b – e ) are provided as a Source data file.

    Article Snippet: Then, each sample was mixed with 2.83 μL of reverse transcription mixture, comprising SuperScript IV reverse transcriptase (Invitrogen, 18090050), RNase inhibitor (Takara, Cat. 2313A), SuperScript IV first-strand buffer, 1 M betaine (Sigma-Aldrich, B0300-1VL), 100 mM DTT (Invitrogen, 18090050), 50 mM MgCl2 (Sangon, A610328-0500), and template-switching oligo primers.

    Techniques: Control, Synthesized, Amplification, Derivative Assay, Purification, Sequencing, Modification, Biomarker Discovery