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truseq custom amplicon kit  (Illumina Inc)


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    Structured Review

    Illumina Inc truseq custom amplicon kit
    Truseq Custom Amplicon Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 95/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fc/TruSeq+Custom+Amplicon+Index+Kit/pmc07016977-76-9-14
    Average 95 stars, based on 141 article reviews
    truseq custom amplicon kit - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Selective amplification of overlapping amplicons
    Article Snippet: .. A representative PCR mixture of 25 μL included the following components: 2.5 μL 10× reaction buffer, 0.5 μL dNTPs, 0.25 μL enzyme (Roche, Cat #12140314001), 2 μL of the diluted product from Example 3, 2 μL of Illumina Index Forward primer, 2 ILL Illumina Index Reverse primer (25 μM primer stock from TruSeq Custom Amplicon Index Kit, Cat #FC-130-1003), and 15.75 μL nuclease-free water. .. PCR amplification was performed as follows: 1 cycle 95° C. 4 minutes Initial DNA denaturation 95° C. 30 seconds Denaturation 20 cycles 66° C. 30 seconds Annealing 72° C. 60 seconds Extension 1 cycle 72° C. 5 minutes Final extension 1 cycle 8° C. Hold The product was purified by adding 18 μL of Agencourt AMPure XP beads (Beckman Coulter, Cat #A63881), separating the beads from the supernatant, and discarding the supernatant.

    Article Title: Molecular surveillance of Plasmodium falciparum drug-resistance markers in Vietnam using multiplex amplicon sequencing (2000–2016)
    Article Snippet: Reagents: • Phi29 polymerase 125 μl - NEB, M0269L • dNTPs (10mM pool -2.5 mM each) • sWGA primer pool (250μM – 25μM each). .. See Oyola et al, Malar J, 2016 and MalariaGEN Resources https://www.malariagen.net/resources/ampliconsequencing-toolkit/p-falciparum-amplicontoolkit-protocols • Agencourt® Ampure® XP beads – Beckman Coulter • Qubit dsDNA HS kit (ThermoFisher) • Truseq Custom Amplicon Low Input Library Prep Kit 96 samples – Custom Design v1_1FC-134-2001 • TruSeq Custom Amplicon Index Kit (96 indexes, 384 samples) – Illumina, FC-1301003 • KAPA quantification kit for LC480 500Rx – Roche Diagnostics KK4854 - 07960298001 • MiSeq Reagent Kit v2 (300-cycles) – Illumina, MS-102-2002 • Tris-HCl • SYBR Safe DNA Gel Stain (ThermoFisher) • DNAZap (Invitrogen) • Nuclease-free water • Agarose Consumables: • Adhesive films Microseal type A • Cap strips • 96-well PCR plates Page 14 of 14 • 96-well plates (2 ml volume/well) • Nuclease-free 1.5 ml tubes • Falcon tubes 50 ml • Filter pipette tips • LightCycler 480 optical qPCR plates Equipment • Plate shaker (1800 rpm) • Multichannel pipette 10 μl • Multichannel pipette 100 μl • Multichannel pipette 300 μl or 1000 μl • Monochannel pipettes • Repeater • Qubit fluorometer • DynaMagTM-96 Side Magnet (Thermo Fisher), or equivalent • Verity thermal cycler (ThermoFisher) • LightCycler480 • Gel electrophoresis tray and equipment ..

    Article Title: Novel method for detecting frequent TERT promoter hot spot mutations in bladder cancer samples.
    Article Snippet: .. The TERTp region was amplified and elongated with an adaptor sequence compatible with the TruSeq Custom Amplicon Index Kit (96 indexes, Illumina) using the same PCR protocol as described in the “Target amplification PCR” section of the manual. ..

    Amplification:

    Article Title: Selective amplification of overlapping amplicons
    Article Snippet: .. A representative PCR mixture of 25 μL included the following components: 2.5 μL 10× reaction buffer, 0.5 μL dNTPs, 0.25 μL enzyme (Roche, Cat #12140314001), 2 μL of the diluted product from Example 3, 2 μL of Illumina Index Forward primer, 2 ILL Illumina Index Reverse primer (25 μM primer stock from TruSeq Custom Amplicon Index Kit, Cat #FC-130-1003), and 15.75 μL nuclease-free water. .. PCR amplification was performed as follows: 1 cycle 95° C. 4 minutes Initial DNA denaturation 95° C. 30 seconds Denaturation 20 cycles 66° C. 30 seconds Annealing 72° C. 60 seconds Extension 1 cycle 72° C. 5 minutes Final extension 1 cycle 8° C. Hold The product was purified by adding 18 μL of Agencourt AMPure XP beads (Beckman Coulter, Cat #A63881), separating the beads from the supernatant, and discarding the supernatant.

    Article Title: Molecular Mapping to Discover Reliable Salinity-Resilient QTLs from the Novel Landrace Akundi in Two Bi-Parental Populations Using SNP-Based Genome-Wide Analysis in Rice
    Article Snippet: .. The 1k-RiCA (1K Rice Custom Amplicon) assay was constructed on Illumina’s TruSeq Custom Amplicon (TSCA) 384 Index Kit technology ( https://www.illumina.com ; accessed on 21 October 2022) using Illumina’s registered workflow [ ]. ..

    Article Title: NGS Sequencing Reveals New UCP1 Gene Variants Potentially Associated with MetS and/or T2DM Risk in the Polish Population-A Preliminary Study.
    Article Snippet: .. To build amplicon sequencing libraries for the coding region of the UCP1 gene, we used TruSeq Custom Amplicon Low Input Kit (Illumina, San Diego, CA, USA) with TruSeq Dual Index Sequencing Primers (Illumina, San Diego, CA, USA). .. For 3′UTR and 5′UTR regions of the UCP1 gene, we used AmpliSeq Illumina Custom DNA Panel (Illumina, San Diego, CA, USA) with AmpliSeq CD Indexes (Illumina, San Diego, CA, USA).

    Article Title: Molecular Mapping to Discover Reliable Salinity-Resilient QTLs from the Novel Landrace Akundi in Two Bi-Parental Populations Using SNP-Based Genome-Wide Analysis in Rice.
    Article Snippet: .. The 1k-RiCA (1K Rice Custom Amplicon) assay was constructed on Illumina’s TruSeq Custom Amplicon (TSCA) 384 Index Kit technology (https://www.illumina.com; accessed on 21 October 2022) using Illumina’s registered workflow [53]. ..

    Article Title: Molecular surveillance of Plasmodium falciparum drug-resistance markers in Vietnam using multiplex amplicon sequencing (2000–2016)
    Article Snippet: Reagents: • Phi29 polymerase 125 μl - NEB, M0269L • dNTPs (10mM pool -2.5 mM each) • sWGA primer pool (250μM – 25μM each). .. See Oyola et al, Malar J, 2016 and MalariaGEN Resources https://www.malariagen.net/resources/ampliconsequencing-toolkit/p-falciparum-amplicontoolkit-protocols • Agencourt® Ampure® XP beads – Beckman Coulter • Qubit dsDNA HS kit (ThermoFisher) • Truseq Custom Amplicon Low Input Library Prep Kit 96 samples – Custom Design v1_1FC-134-2001 • TruSeq Custom Amplicon Index Kit (96 indexes, 384 samples) – Illumina, FC-1301003 • KAPA quantification kit for LC480 500Rx – Roche Diagnostics KK4854 - 07960298001 • MiSeq Reagent Kit v2 (300-cycles) – Illumina, MS-102-2002 • Tris-HCl • SYBR Safe DNA Gel Stain (ThermoFisher) • DNAZap (Invitrogen) • Nuclease-free water • Agarose Consumables: • Adhesive films Microseal type A • Cap strips • 96-well PCR plates Page 14 of 14 • 96-well plates (2 ml volume/well) • Nuclease-free 1.5 ml tubes • Falcon tubes 50 ml • Filter pipette tips • LightCycler 480 optical qPCR plates Equipment • Plate shaker (1800 rpm) • Multichannel pipette 10 μl • Multichannel pipette 100 μl • Multichannel pipette 300 μl or 1000 μl • Monochannel pipettes • Repeater • Qubit fluorometer • DynaMagTM-96 Side Magnet (Thermo Fisher), or equivalent • Verity thermal cycler (ThermoFisher) • LightCycler480 • Gel electrophoresis tray and equipment ..

    Article Title: Molecular Mapping To Identify Stable Salinity-Resilient QTLs From the Novel Landrace Akundi in Two Bi-Parental Populations Using SNP-Based 1k-RiCA Genotyping in Rice
    Article Snippet: .. The 1k-RiCA (1K Rice Custom Amplicon) assay was constructed on Illumina’s TruSeq Custom Amplicon (TSCA) 384 Index Kit technology (https://www.illumina.com) using Illumina’s registered workflow [25]. ..

    Article Title: Molecular profiling of rice (Oryza sativa L.) genotypes using trait-based SNP markers linked to yield under drought condition
    Article Snippet: .. DNA extraction and genotyping The 1k-RiCA (Rice Custom Amplicon assay), which was designed using Illumina’s TruSeq Custom Amplicon (TSCA) 384 Index Kit technology (https://www.illumina.com) was used for genotyping of the population. ..

    Article Title: Novel method for detecting frequent TERT promoter hot spot mutations in bladder cancer samples.
    Article Snippet: .. The TERTp region was amplified and elongated with an adaptor sequence compatible with the TruSeq Custom Amplicon Index Kit (96 indexes, Illumina) using the same PCR protocol as described in the “Target amplification PCR” section of the manual. ..

    Construct:

    Article Title: Molecular Mapping to Discover Reliable Salinity-Resilient QTLs from the Novel Landrace Akundi in Two Bi-Parental Populations Using SNP-Based Genome-Wide Analysis in Rice
    Article Snippet: .. The 1k-RiCA (1K Rice Custom Amplicon) assay was constructed on Illumina’s TruSeq Custom Amplicon (TSCA) 384 Index Kit technology ( https://www.illumina.com ; accessed on 21 October 2022) using Illumina’s registered workflow [ ]. ..

    Article Title: Molecular Mapping to Discover Reliable Salinity-Resilient QTLs from the Novel Landrace Akundi in Two Bi-Parental Populations Using SNP-Based Genome-Wide Analysis in Rice.
    Article Snippet: .. The 1k-RiCA (1K Rice Custom Amplicon) assay was constructed on Illumina’s TruSeq Custom Amplicon (TSCA) 384 Index Kit technology (https://www.illumina.com; accessed on 21 October 2022) using Illumina’s registered workflow [53]. ..

    Article Title: Molecular Mapping To Identify Stable Salinity-Resilient QTLs From the Novel Landrace Akundi in Two Bi-Parental Populations Using SNP-Based 1k-RiCA Genotyping in Rice
    Article Snippet: .. The 1k-RiCA (1K Rice Custom Amplicon) assay was constructed on Illumina’s TruSeq Custom Amplicon (TSCA) 384 Index Kit technology (https://www.illumina.com) using Illumina’s registered workflow [25]. ..

    Sequencing:

    Article Title: NGS Sequencing Reveals New UCP1 Gene Variants Potentially Associated with MetS and/or T2DM Risk in the Polish Population-A Preliminary Study.
    Article Snippet: .. To build amplicon sequencing libraries for the coding region of the UCP1 gene, we used TruSeq Custom Amplicon Low Input Kit (Illumina, San Diego, CA, USA) with TruSeq Dual Index Sequencing Primers (Illumina, San Diego, CA, USA). .. For 3′UTR and 5′UTR regions of the UCP1 gene, we used AmpliSeq Illumina Custom DNA Panel (Illumina, San Diego, CA, USA) with AmpliSeq CD Indexes (Illumina, San Diego, CA, USA).

    Article Title: Novel method for detecting frequent TERT promoter hot spot mutations in bladder cancer samples.
    Article Snippet: .. The TERTp region was amplified and elongated with an adaptor sequence compatible with the TruSeq Custom Amplicon Index Kit (96 indexes, Illumina) using the same PCR protocol as described in the “Target amplification PCR” section of the manual. ..

    Staining:

    Article Title: Molecular surveillance of Plasmodium falciparum drug-resistance markers in Vietnam using multiplex amplicon sequencing (2000–2016)
    Article Snippet: Reagents: • Phi29 polymerase 125 μl - NEB, M0269L • dNTPs (10mM pool -2.5 mM each) • sWGA primer pool (250μM – 25μM each). .. See Oyola et al, Malar J, 2016 and MalariaGEN Resources https://www.malariagen.net/resources/ampliconsequencing-toolkit/p-falciparum-amplicontoolkit-protocols • Agencourt® Ampure® XP beads – Beckman Coulter • Qubit dsDNA HS kit (ThermoFisher) • Truseq Custom Amplicon Low Input Library Prep Kit 96 samples – Custom Design v1_1FC-134-2001 • TruSeq Custom Amplicon Index Kit (96 indexes, 384 samples) – Illumina, FC-1301003 • KAPA quantification kit for LC480 500Rx – Roche Diagnostics KK4854 - 07960298001 • MiSeq Reagent Kit v2 (300-cycles) – Illumina, MS-102-2002 • Tris-HCl • SYBR Safe DNA Gel Stain (ThermoFisher) • DNAZap (Invitrogen) • Nuclease-free water • Agarose Consumables: • Adhesive films Microseal type A • Cap strips • 96-well PCR plates Page 14 of 14 • 96-well plates (2 ml volume/well) • Nuclease-free 1.5 ml tubes • Falcon tubes 50 ml • Filter pipette tips • LightCycler 480 optical qPCR plates Equipment • Plate shaker (1800 rpm) • Multichannel pipette 10 μl • Multichannel pipette 100 μl • Multichannel pipette 300 μl or 1000 μl • Monochannel pipettes • Repeater • Qubit fluorometer • DynaMagTM-96 Side Magnet (Thermo Fisher), or equivalent • Verity thermal cycler (ThermoFisher) • LightCycler480 • Gel electrophoresis tray and equipment ..

    Adhesive:

    Article Title: Molecular surveillance of Plasmodium falciparum drug-resistance markers in Vietnam using multiplex amplicon sequencing (2000–2016)
    Article Snippet: Reagents: • Phi29 polymerase 125 μl - NEB, M0269L • dNTPs (10mM pool -2.5 mM each) • sWGA primer pool (250μM – 25μM each). .. See Oyola et al, Malar J, 2016 and MalariaGEN Resources https://www.malariagen.net/resources/ampliconsequencing-toolkit/p-falciparum-amplicontoolkit-protocols • Agencourt® Ampure® XP beads – Beckman Coulter • Qubit dsDNA HS kit (ThermoFisher) • Truseq Custom Amplicon Low Input Library Prep Kit 96 samples – Custom Design v1_1FC-134-2001 • TruSeq Custom Amplicon Index Kit (96 indexes, 384 samples) – Illumina, FC-1301003 • KAPA quantification kit for LC480 500Rx – Roche Diagnostics KK4854 - 07960298001 • MiSeq Reagent Kit v2 (300-cycles) – Illumina, MS-102-2002 • Tris-HCl • SYBR Safe DNA Gel Stain (ThermoFisher) • DNAZap (Invitrogen) • Nuclease-free water • Agarose Consumables: • Adhesive films Microseal type A • Cap strips • 96-well PCR plates Page 14 of 14 • 96-well plates (2 ml volume/well) • Nuclease-free 1.5 ml tubes • Falcon tubes 50 ml • Filter pipette tips • LightCycler 480 optical qPCR plates Equipment • Plate shaker (1800 rpm) • Multichannel pipette 10 μl • Multichannel pipette 100 μl • Multichannel pipette 300 μl or 1000 μl • Monochannel pipettes • Repeater • Qubit fluorometer • DynaMagTM-96 Side Magnet (Thermo Fisher), or equivalent • Verity thermal cycler (ThermoFisher) • LightCycler480 • Gel electrophoresis tray and equipment ..

    Transferring:

    Article Title: Molecular surveillance of Plasmodium falciparum drug-resistance markers in Vietnam using multiplex amplicon sequencing (2000–2016)
    Article Snippet: Reagents: • Phi29 polymerase 125 μl - NEB, M0269L • dNTPs (10mM pool -2.5 mM each) • sWGA primer pool (250μM – 25μM each). .. See Oyola et al, Malar J, 2016 and MalariaGEN Resources https://www.malariagen.net/resources/ampliconsequencing-toolkit/p-falciparum-amplicontoolkit-protocols • Agencourt® Ampure® XP beads – Beckman Coulter • Qubit dsDNA HS kit (ThermoFisher) • Truseq Custom Amplicon Low Input Library Prep Kit 96 samples – Custom Design v1_1FC-134-2001 • TruSeq Custom Amplicon Index Kit (96 indexes, 384 samples) – Illumina, FC-1301003 • KAPA quantification kit for LC480 500Rx – Roche Diagnostics KK4854 - 07960298001 • MiSeq Reagent Kit v2 (300-cycles) – Illumina, MS-102-2002 • Tris-HCl • SYBR Safe DNA Gel Stain (ThermoFisher) • DNAZap (Invitrogen) • Nuclease-free water • Agarose Consumables: • Adhesive films Microseal type A • Cap strips • 96-well PCR plates Page 14 of 14 • 96-well plates (2 ml volume/well) • Nuclease-free 1.5 ml tubes • Falcon tubes 50 ml • Filter pipette tips • LightCycler 480 optical qPCR plates Equipment • Plate shaker (1800 rpm) • Multichannel pipette 10 μl • Multichannel pipette 100 μl • Multichannel pipette 300 μl or 1000 μl • Monochannel pipettes • Repeater • Qubit fluorometer • DynaMagTM-96 Side Magnet (Thermo Fisher), or equivalent • Verity thermal cycler (ThermoFisher) • LightCycler480 • Gel electrophoresis tray and equipment ..

    Real-time Polymerase Chain Reaction:

    Article Title: Molecular surveillance of Plasmodium falciparum drug-resistance markers in Vietnam using multiplex amplicon sequencing (2000–2016)
    Article Snippet: Reagents: • Phi29 polymerase 125 μl - NEB, M0269L • dNTPs (10mM pool -2.5 mM each) • sWGA primer pool (250μM – 25μM each). .. See Oyola et al, Malar J, 2016 and MalariaGEN Resources https://www.malariagen.net/resources/ampliconsequencing-toolkit/p-falciparum-amplicontoolkit-protocols • Agencourt® Ampure® XP beads – Beckman Coulter • Qubit dsDNA HS kit (ThermoFisher) • Truseq Custom Amplicon Low Input Library Prep Kit 96 samples – Custom Design v1_1FC-134-2001 • TruSeq Custom Amplicon Index Kit (96 indexes, 384 samples) – Illumina, FC-1301003 • KAPA quantification kit for LC480 500Rx – Roche Diagnostics KK4854 - 07960298001 • MiSeq Reagent Kit v2 (300-cycles) – Illumina, MS-102-2002 • Tris-HCl • SYBR Safe DNA Gel Stain (ThermoFisher) • DNAZap (Invitrogen) • Nuclease-free water • Agarose Consumables: • Adhesive films Microseal type A • Cap strips • 96-well PCR plates Page 14 of 14 • 96-well plates (2 ml volume/well) • Nuclease-free 1.5 ml tubes • Falcon tubes 50 ml • Filter pipette tips • LightCycler 480 optical qPCR plates Equipment • Plate shaker (1800 rpm) • Multichannel pipette 10 μl • Multichannel pipette 100 μl • Multichannel pipette 300 μl or 1000 μl • Monochannel pipettes • Repeater • Qubit fluorometer • DynaMagTM-96 Side Magnet (Thermo Fisher), or equivalent • Verity thermal cycler (ThermoFisher) • LightCycler480 • Gel electrophoresis tray and equipment ..

    Nucleic Acid Electrophoresis:

    Article Title: Molecular surveillance of Plasmodium falciparum drug-resistance markers in Vietnam using multiplex amplicon sequencing (2000–2016)
    Article Snippet: Reagents: • Phi29 polymerase 125 μl - NEB, M0269L • dNTPs (10mM pool -2.5 mM each) • sWGA primer pool (250μM – 25μM each). .. See Oyola et al, Malar J, 2016 and MalariaGEN Resources https://www.malariagen.net/resources/ampliconsequencing-toolkit/p-falciparum-amplicontoolkit-protocols • Agencourt® Ampure® XP beads – Beckman Coulter • Qubit dsDNA HS kit (ThermoFisher) • Truseq Custom Amplicon Low Input Library Prep Kit 96 samples – Custom Design v1_1FC-134-2001 • TruSeq Custom Amplicon Index Kit (96 indexes, 384 samples) – Illumina, FC-1301003 • KAPA quantification kit for LC480 500Rx – Roche Diagnostics KK4854 - 07960298001 • MiSeq Reagent Kit v2 (300-cycles) – Illumina, MS-102-2002 • Tris-HCl • SYBR Safe DNA Gel Stain (ThermoFisher) • DNAZap (Invitrogen) • Nuclease-free water • Agarose Consumables: • Adhesive films Microseal type A • Cap strips • 96-well PCR plates Page 14 of 14 • 96-well plates (2 ml volume/well) • Nuclease-free 1.5 ml tubes • Falcon tubes 50 ml • Filter pipette tips • LightCycler 480 optical qPCR plates Equipment • Plate shaker (1800 rpm) • Multichannel pipette 10 μl • Multichannel pipette 100 μl • Multichannel pipette 300 μl or 1000 μl • Monochannel pipettes • Repeater • Qubit fluorometer • DynaMagTM-96 Side Magnet (Thermo Fisher), or equivalent • Verity thermal cycler (ThermoFisher) • LightCycler480 • Gel electrophoresis tray and equipment ..

    DNA Extraction:

    Article Title: Molecular profiling of rice (Oryza sativa L.) genotypes using trait-based SNP markers linked to yield under drought condition
    Article Snippet: .. DNA extraction and genotyping The 1k-RiCA (Rice Custom Amplicon assay), which was designed using Illumina’s TruSeq Custom Amplicon (TSCA) 384 Index Kit technology (https://www.illumina.com) was used for genotyping of the population. ..



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