fc receptor blocking reagent (Miltenyi Biotec)
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Miltenyi Biotec
fc receptor blocking reagent
Fc Receptor Blocking Reagent, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fc/FcR+Blocking+Reagent%2C+mouse/pm42414546-268-0-4
Average 97 stars, based on 1263 article reviews
Fc Receptor Blocking Reagent, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fc/FcR+Blocking+Reagent%2C+mouse/pm42414546-268-0-4
Average 97 stars, based on 1263 article reviews
fc receptor blocking reagent - by Bioz Stars,
2026-09
97/100 stars
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Staining:Article Title: Tracking B cell immunity during perturbation of hepatitis B infection induced by treatment withdrawal Article Snippet: Cells were stained with Fixable Live/Dead Dye (Life Technologies, Thermo Fisher Scientific) before incubation with saturating concentrations of surface mAbs diluted in 50% Brilliant Violet Buffer (BD Biosciences) and 50% phosphate-buffered saline (PBS) for 30 min at 4°C. .. In all instances, cells were stained in the presence of Incubation:Article Title: Humans homozygous for rare or common hypomorphic IL23R variants are prone to tuberculosis Article Snippet: .. They were then permeabilized/stained by incubation overnight at −20°C in the permeabilization buffer from the Nuclear Transcription Factor Buffer Set (BioLegend) with an intracellular cytokine panel containing Article Title: Identification of immunopeptides (pHLA) as candidate therapeutic targets in chondrosarcoma Article Snippet: Cell concentration was adjusted to 1 × 10 6 cells/mL in PBS and cells were incubated with viobility dye (VioGreen, Miltenyi Biotec) for 20 min in the dark. .. Cells were then washed twice with FACS buffer (0,1% BSA, 0,5 mM EDTA), incubated with Article Title: Tumor cell-released autophagosome (TRAP) programs inflammatory CAFs to drive the immune-excluded TIME through C3a. Article Snippet: The immune-excluded tumor immune microenvironment (TIME) limits responses to ICIs.. Cancer-associated fibroblasts are the most abundant stromal population and key regulators of immune suppression; however, the upstream cues that program pathogenic CAF states and the mechanisms of the immune-excluded TIME remain poorly defined.. Here, by combining single-cell RNA sequencing and functional validation, we report that tumor cell-released autophagosome (TRAP) programs inflammatory CAFs (iCAFs) and triggers cathepsin L-dependent intracellular cleavage of C3 into C3a via the HSP70–TLR4–MyD88–ERK/p38 pathway. iCAF-derived C3a affects C3a on TAMs, promotes TAM accumulation in the iCAF-rich stroma, limits TIL trafficking into tumor nests, and reinforces an immune-excluded TIME. Article Title: Distinct systemic and gut IgA responses to bacteria of the human upper gastrointestinal tract. Article Snippet: In addition, a barcoded anti-human CD27 antibody (TotalSeq-C0154, BioLegend) was included, and BM and gut cell suspensions were labeled separately with anti-human Hashtag 1 (TotalSeqC0251, BioLegend) and Hashtag 2 (TotalSeq-C0252, BioLegend) antibodies, respectively. .. The cells were incubated with Blocking Assay:Article Title: Humans homozygous for rare or common hypomorphic IL23R variants are prone to tuberculosis Article Snippet: .. They were then permeabilized/stained by incubation overnight at −20°C in the permeabilization buffer from the Nuclear Transcription Factor Buffer Set (BioLegend) with an intracellular cytokine panel containing Article Title: Identification of immunopeptides (pHLA) as candidate therapeutic targets in chondrosarcoma Article Snippet: Cell concentration was adjusted to 1 × 10 6 cells/mL in PBS and cells were incubated with viobility dye (VioGreen, Miltenyi Biotec) for 20 min in the dark. .. Cells were then washed twice with FACS buffer (0,1% BSA, 0,5 mM EDTA), incubated with Article Title: Tumor cell-released autophagosome (TRAP) programs inflammatory CAFs to drive the immune-excluded TIME through C3a. Article Snippet: The immune-excluded tumor immune microenvironment (TIME) limits responses to ICIs.. Cancer-associated fibroblasts are the most abundant stromal population and key regulators of immune suppression; however, the upstream cues that program pathogenic CAF states and the mechanisms of the immune-excluded TIME remain poorly defined.. Here, by combining single-cell RNA sequencing and functional validation, we report that tumor cell-released autophagosome (TRAP) programs inflammatory CAFs (iCAFs) and triggers cathepsin L-dependent intracellular cleavage of C3 into C3a via the HSP70–TLR4–MyD88–ERK/p38 pathway. iCAF-derived C3a affects C3a on TAMs, promotes TAM accumulation in the iCAF-rich stroma, limits TIL trafficking into tumor nests, and reinforces an immune-excluded TIME. Article Title: Distinct systemic and gut IgA responses to bacteria of the human upper gastrointestinal tract. Article Snippet: In addition, a barcoded anti-human CD27 antibody (TotalSeq-C0154, BioLegend) was included, and BM and gut cell suspensions were labeled separately with anti-human Hashtag 1 (TotalSeqC0251, BioLegend) and Hashtag 2 (TotalSeq-C0252, BioLegend) antibodies, respectively. .. The cells were incubated with Article Title: Poly(I:C) and QS-21 combination suppresses breast tumor growth and metastasis by repolarizing tumor associated macrophages to anti-tumor macrophages. Article Snippet: Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer defined by a paucity of targeted therapies, high metastatic propensity, and an immunosuppressive tumor microenvironment rich in tumor-associated macrophages (TAMs).. Here, we developed a liposomal immunoadjuvant system co-encapsulating Poly(I:C) and QS-21 aimed at reprogramming TAMs and bolstering antitumor immunity.. In an orthotopic 4T1 TNBC mouse model, intratumoral injection of the combined formulation markedly suppressed primary tumor growth and pulmonary metastasis, with no observable systemic toxicity. FACS:Article Title: Identification of immunopeptides (pHLA) as candidate therapeutic targets in chondrosarcoma Article Snippet: Cell concentration was adjusted to 1 × 10 6 cells/mL in PBS and cells were incubated with viobility dye (VioGreen, Miltenyi Biotec) for 20 min in the dark. .. Cells were then washed twice with FACS buffer (0,1% BSA, 0,5 mM EDTA), incubated with Single Cell:Article Title: Tumor cell-released autophagosome (TRAP) programs inflammatory CAFs to drive the immune-excluded TIME through C3a. Article Snippet: The immune-excluded tumor immune microenvironment (TIME) limits responses to ICIs.. Cancer-associated fibroblasts are the most abundant stromal population and key regulators of immune suppression; however, the upstream cues that program pathogenic CAF states and the mechanisms of the immune-excluded TIME remain poorly defined.. Here, by combining single-cell RNA sequencing and functional validation, we report that tumor cell-released autophagosome (TRAP) programs inflammatory CAFs (iCAFs) and triggers cathepsin L-dependent intracellular cleavage of C3 into C3a via the HSP70–TLR4–MyD88–ERK/p38 pathway. iCAF-derived C3a affects C3a on TAMs, promotes TAM accumulation in the iCAF-rich stroma, limits TIL trafficking into tumor nests, and reinforces an immune-excluded TIME. Article Title: Poly(I:C) and QS-21 combination suppresses breast tumor growth and metastasis by repolarizing tumor associated macrophages to anti-tumor macrophages. Article Snippet: Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer defined by a paucity of targeted therapies, high metastatic propensity, and an immunosuppressive tumor microenvironment rich in tumor-associated macrophages (TAMs).. Here, we developed a liposomal immunoadjuvant system co-encapsulating Poly(I:C) and QS-21 aimed at reprogramming TAMs and bolstering antitumor immunity.. In an orthotopic 4T1 TNBC mouse model, intratumoral injection of the combined formulation markedly suppressed primary tumor growth and pulmonary metastasis, with no observable systemic toxicity. |